cell based screening in antibacterial discovery

... (5% BSA in PBS‐T)  and  washed  thrice in PBS. For  nuclear  staining,  cells  were  incubated  in 400  nM  DAPI  stain  in RT  for  30  minutes  and  washed  thrice  in PBS.  Infected  cells  ... is  necessary  to  optimize  these  cell based screening assays  for  inhibitors  of  PCV2  replication.      Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication  ... SIV    Swine Influenza Virus  SPA    Scintillation Proximity Assay  TCID50    50% Tissue Culture Infective Dose   uHTS    Ultra High‐Throughput Screening Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication ...

Ngày tải lên: 03/10/2015, 11:36

... replication. The assay was developed by monitoring the expression of Rep protein through IFA to assess  PCV2 replication. Screening assays in drug discovery are generally divided  into cell based and cell free  formats.  In this  ... difficulty, and scaling down to the 384‐well plate format was expected to be straightforward. Optimizing  required tweaking three parameters: 1) cell seeding density, 2) MOI, and 3) duration of infection prior to  fixation.   Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication     ... attempt  to  increase  infection  rate  by  enhancing  host‐virus  interaction  was  by  pre‐ incubating  PCV2  and  PK15‐C1  in suspension  prior  to  seeding.  The  rocking  motion  during  incubation ...

Ngày tải lên: 13/10/2015, 16:41

... nephrotoxicity and gastrointestinal toxicity than CDDP [14] In phase II clinical studies, NDP was found to be highly effective against solid tumors, including non-small cell lung cancer, small cell lung cancer, ... toxicity in the treatment of ESCC [16] In this study, the effects of replacing CDDP with NDP on the pharmacokinetics of 5-FU 306 were investigated in ESCC patients treated with a definitive 5-FU/CDDP -based ... cancer, and treatments include surgical procedures, radiation, chemotherapy, and combinations thereof [10] In patients with localized squamous cell carcinoma, a definitive 5-FU/CDDP -based CRT is one...

Ngày tải lên: 26/10/2012, 09:53

... future inhibitors designed for this NF-jB signaling pathway should be tested not just in vitro and cell based but also simulated in silico Combining this method of analysis (in vitro, cell- based ... nuclear NF-jB in single cell studies In our cell- based experiments, a population of 10 000 A549 cells was observed to undergo similar oscillatory behavior to that discovered in single cells in terms ... lL; prewarmed in a water bath at 37 C) was dispensed into each well and incubated for 15 (prewarming xative is critical to maintaining cell integrity) After 15 min, the plates were inverted to...

Ngày tải lên: 16/03/2014, 11:20

... endocrine disruptors in cell lines [10] We know that xenobiotics have a main endpoint in the liver, which is the major detoxification organ Here, we investigated the mechanism of action of R in ... concentration was determined in each S9 fraction according to the Bicinchoninic Acid Protein Assay (Sigma, France) Cell death measurement The enzymatic MTT test is based on the cleavage of MTT into blue formazan ... not prevent GST inhibition by R that could lead to accumulation of toxic reactive compounds Since the use of cell lines allows longer experiments, and since cell lines may in some instances be less...

Ngày tải lên: 20/06/2014, 00:20

... in infected and uninfected cells in the presence of selected compounds NFκB activation in infected and uninfected cells in the presence of selected compounds Infected or mock infected MA104 cells ... × 105 U/ml in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) Recombinant human interferon-γ (IFN-γ) and recombinant human interleukin2 (IL-2; Peprotech Inc) were diluted ... prostaglandin synthesis is necessary for rotavirus infectivity in CaCo-2 intestinal cells [19] Interestingly, the addition of prostaglandin E2 (PGE2) restored infectivity reduced by the COX inhibitor...

Ngày tải lên: 20/06/2014, 01:20

... according to infit and outfit statistics, strong evidence for unidimensionality and local independence, and no signs of differential item functioning Keeping in mind that the DESC’s validity in clinical ... was originally developed for depression screening in patients with somatic diseases, which is also one the main fields of application for the DESC Furthermore, it provides screening information ... be examined simultaneously Table Item characteristics of the Rasch -based Depression Screening I (DESC-I) and the Rasch -based Depression Screening II (DESC-II) Item δi * S.E Threshold infit

Ngày tải lên: 20/06/2014, 16:20

... Discussion Originally, 5-FU was administered alone as a bolus, but more recently, it is being administered with biomodulating agents and/or through continuous infusion [11,33] Because of the preclinical ... Average of sampling points a) Survival of years or more vs less than years 5-FU is extensive inter- and/or intra-individual variability in pharmacokinetics, when doses are calculated based on body ... I, Tamura T, Inokuma T, Takemoto Y, Nakamura T, Kataoka K, Sakaeda T: Replacement of cisplatin with nedaplatin in a definitive 5-fluorouracil/ cisplatin -based chemoradiotherapy in Japanese patients...

Ngày tải lên: 10/08/2014, 10:21

... below) were maintained in same cell culture media that contains 100 µg/mL hygromycin B and 300 µg/mL G418 MCF-7 breast adenocarcinoma cells were maintained and grown as monolayer cultures in phenol ... post-transfection, the cells were washed, trypsinized and split at ratio of in 10, in 100 and in 1000 into selective media containing 100 μg/mL of hygromycin B The diluted cells were re-seeded ... trypsinized and split at ratio of in 10, in 100 and in 1000 into selective cell culture media containing 100 μg/mL of hygromycin B and 300 μg/mlL of G418 The diluted cells were re-seeded onto a new...

Ngày tải lên: 10/09/2015, 15:48

... bioactivities, in terms of EC50 values and peak activities were - apigenin>kaempferol=luteolin>quercetin corresponding to increasing number of hydroxyl groups Apigenin, kaempferol and luteolin induced ... Combinatorial effects of binary mixtures of estradiol with genistein and Epimedium prenylflavonoids To study combinatorial effects of flavonoids and estradiol, binary mixtures containing increasing ... genistein and Epimedium prenylflavonoids ERα stable cells were incubated with increasing doses of genistein (A), icariin (B), icariside I (C), icariside II (D), icaritin (E) and desmethylicaritin...

Ngày tải lên: 10/09/2015, 15:54

... the sex 201 hormone-binding globulin Hence, it is not likely that icaritin will bind to the sex hormone-binding globulin protein found in serum The late appearance of icaritin in sera of human volunteers ... icariin to icaritin which occurs mainly in the intestine This view is supported by animal data showing that icariin is stable in gastric juice, and that hydrolysis of icariin to icaritin occurs in ... 2004) The presence of serum reduced ERα binding of quercetin in MCF-7 cells, with relative binding affinities for genistein, kaempferol, and quercetin being 0.1, 0.012, and 0.001, respectively...

Ngày tải lên: 10/09/2015, 15:54

... that in Traditional Chinese Medicine, medicinal herbs are rarely taken via a single dose In the Bencao Gangmu (本草纲目), a Chinese materia medica work written by Li Shizhen (李 时珍) in the Ming dynasty, ... (-)-epigallocatechin-3-gallate significantly increased the biochanin A area under the plasma concentration versus time curve in rats in both intravenous and oral administration of biochanin A According to ... of such cell- based bioassays Even within the same cell line, differences exist in the ability of different compounds to induce estrogen-regulated gene expression as seen in Ishikawa cells where...

Ngày tải lên: 10/09/2015, 15:54

... of active ingredients in traditional Chinese herbal drugs Assay of icariin in Epimedium Chin J Pharm Anal 4: 81-84 Liu, J and Lou, Y.J 2004 Determination of icariin and metabolites in rat serum ... flavanone aglycones hesperetin and naringenin after single oral administration in human subjects Eur J Clin Nutr 61(4):472-7 Klinge, C.M 2000 Estrogen receptor interaction with co-activators and ... determination of serum estrogenic bioactivity in children J Clin Endocrinol Metab 87:791–797 Pasqualini, J.R 2005 Enzymes involved in the formation and transformation of steroid hormones in the...

Ngày tải lên: 10/09/2015, 15:54

... hepatocytes 34 Glucose-induced insulin secretion 36 Zinc-induced insulin secretion 37 Kinetics of glucose-induced insulin secretion 39 Kinetics of glucose-induced insulin mRNA expression 41 Induction of ... differentiated in vitro and in vivo into insulin-expressing cells [64-66] Other tissues that have been investigated for progenitor cells that can transdifferentiate to insulinproducing cells or cells ... differentiation into an insulin-producing cellular phenotype [47, 48] 10 However, as is common with studies using stem cells, the cellular identity of these insulin-expressing cells is uncertain and there...

Ngày tải lên: 03/10/2015, 11:36

... Generic.IntLib to join the buses Modify the parameter text to read [9 0] on both sides of the joiner by using inline text editing mode Place a GND power port (from the Wiring toolbar) on the EA pin ... Implementing an 8-bit Processor -based Design in an FPGA Set a memory model by clicking on C Compiler in the Build Options list, selecting Memory Model and choosing Small: variables in DATA from ... Implementing an 8-bit Processor -based Design in an FPGA Figure Initial placement of components on the schematic sheet, Sheet1.SchDoc Wire the design initially, as shown in Figure 2, by using the...

Ngày tải lên: 17/08/2012, 09:12

... time into four activities: interpreting bytecodes while not recording, recording traces (including time taken to interpret the recorded trace), compiling traces to native code, and executing native ... tracing to Java in order to achieve inlining, indirect jump elimination, and other optimizations Their primary focus was on designing an interpreter that could easily be gradually re-engineered ... effects of inlining Because traces have no internal control-flow joins, they can be optimized in linear time by a simple compiler (10) Thus, our tracing VM efficiently performs the same kind of optimizations...

Ngày tải lên: 26/06/2013, 12:43

... E-commerce: buying and selling via the Internet; Supply chain integration: collaboration throughout the total value chain; and Fully integrated e-business: internal and external integration sharing real-time ... hiring, training, retaining, and removing need to be adopted, including the encouragement of innovation and flexibility Knowledge Facilitator The HR department has a role to play in facilitating ... arranging appropriate induction, providing access to information, investing in employee development, being a responsible employer with regard to work-life balance, and being honest regarding job...

Ngày tải lên: 24/10/2013, 08:20

... avail- Protein Name Cyclin dependant kinase Cell cycle regulating D-cyclin Cyclin E2 Proliferation of germ cells (POG) Cyclin D-dependant kinase inhibitors p27 Kip1 a Cdk inhibitor Cdk2 CyclinD2 Gcd/Pog ... partner, cyclin D, are responsible for the initial phosphorylation of the Rb protein by regulating cyclin E/Cdk2 and lifting the S-phase inhibition by preventing binding of its inhibitor, p27 ... spermatids) These intracellular signaling molecules in their absence cause impaired folliculogenesis resulting in a germ cell deficiency within the gonads Effect on germ cell development Sertoli cell specific...

Ngày tải lên: 25/10/2013, 21:20