ROLES OF HUNTINGTIN ASSOCIATED PROTEIN 1 IN INSULIN SECRETING CELLS

... Allowing two substitutions Allowing two substitutions Allowing one substitution AAAGACATG AAAGACAGG AAAGAGATT AAAGAGATG AAAGACATA AAAGACATA AAAGAGAAC AAAGACATA AAAGAAAAG AAACAGATG AAAGAAAAG AAAGAAAAG ... EMSA Result Average Kd (nM) AAAGACATG AGAGACATG AAGGACATG AAATACATG AAAGCCATG AAAGAGATG AAAGACCTG AAAGACACG AAAGACATA + ND ND – – + ND ND ND + – – – – + – + – 0.25 1.5 p=0.01 60_HI 60M_HI Fig...

Ngày tải lên: 07/03/2014, 10:20

... requires protein translocation to the nucleus Although functional analyses of individual domains have not been addressed, the global context of the domain analyses allows us to draw a more general ... 0.75 are shown Black geometrical shapes are additional domains, as indicated ANOGA, Anopheles gambiae; ASHGOS, Ashbya gossypii; BRARE, Brachydanio rerio; CANDGLA, Candi...

Ngày tải lên: 07/03/2014, 21:20

... Ha and side chain protons The spin systems of Lys10 and Arg11 are indicated by rectangles as both contain a second HN in the side chain The N-terminal Gly1 appears as a weak and very broad peak ... FEBS 2004 Recombinant mutant of surfactant protein C (Eur J Biochem 271) 2077 Table Amino-acid sequences of several SP -C polypeptides, including human, porcine and re...

Ngày tải lên: 16/03/2014, 16:20

... Htt-interacting protein HIP -1 and its molecular partner HIPPI in the regulation of apoptosis and transcription, the two processes that are altered in HD [7,8] HIP -1 – its interacting partners and ... Conclusions HIP -1 and its interacting partner HIPPI together induce apoptosis by the intrinsic and extrinsic pathways Homer 1c, an interactor...

Ngày tải lên: 23/03/2014, 07:20

... et al TSC2 promotes the degradation of DAPK Introduction Death-associated protein kinase-1 (DAPK) is the prototypic member of a family of death-related kinases that includes DAPK-1-related protein ... 12, which then binds to and inactivates mTORC1, leading to an upregulation of autophagy [25] Thus mTORC1 acts as a central regulator balancing anabolic and cataboli...

Ngày tải lên: 28/03/2014, 23:20

... Janus kinase ⁄ signal transducer and activator of transcription signaling pathway mediate the regulation of systemic and tissue localized renin -angiotensin system Mol Endocrinol 18 , 10 33 10 41 11 Xu ... studies indicate that transcription activation by STATs requires activated AP -1 [11 13 ] AP -1 is a complex composed of the Fos and Jun proteins [14 16 ] In...

Ngày tải lên: 30/03/2014, 02:20

... Participation of MCP-induced protein in lipopolysaccharide preconditioninginduced ischemic stroke tolerance by regulating the expression of proinflammatory cytokines Jian Liang 1* , Jing ... 2003, 9: 11 80 -11 86 41 Wellen KE, Hotamisligil GS: Inflammation, stress, and diabetes J Clin Invest 2005, 11 5: 11 11- 111 9 25 Figure Legends Figure MCPIP1 mRNA and...

Ngày tải lên: 19/06/2014, 22:20

... 3 .1 Mia1p in assembly and maintenance of persisent iMTOCs at nuclear envelope 44 3 .1. 1 Lack of microtubule binding protein Mia1p results in fewer interphase Microtubules 44 3 .1. 1 .1 Mia1p is a microtubule ... cytoskeleton 18 18 1. 2.4 .1. 1 Architecture and dynamics of interphase microtubule cytoskeleton 18 1. 2.4 .1. 2 Functions of interphase microt...

Ngày tải lên: 12/09/2015, 08:19

... center and plus-ends facing toward the cell tips Model of maintaining ordered microtubules (from Sawin and Tran, 20 06) is shown in the following cartoon 16 Model of interphase microtubules bundling ... critical role in maintaining cell morphology by binding to the plus ends of growing interphase microtubules in a Tip1p and Tea2p-dependent manner (Chang and Peter, 20 03)...

Ngày tải lên: 12/09/2015, 08:19

... 40μl TE buffer containing RNAse and incubated at 37 oC for 20min DNA was stored at -20oC 2 .3 Construction of knock out mutants and epitope tagging of genes 2 .3. 1 Construction of knock out mutants ... taxol-stabilized bovine brain 3X microtubules (50μg/ml) in the same buffer, followed by washing and immunoblotting withTAT1 anti-tubulin antibodies 2.2.5 Extraction of S .pom...

Ngày tải lên: 12/09/2015, 08:19

... kinetochore in metaphase (~1% of cells) and to the spindle midzone during anaphase (~5% of cells) in control cells Ark1p-GFP defined the spindle midzone in 43 % of Mia1p- overexpressing cells, and ... panel) and cells overexpressing Mia1p (right panel) coexpressing the NE marker protein, Uch2p-GFP, and the SPB protein, Sad1p-DSRed 84 3.2.3 Spindles in Mia1p- ov...

Ngày tải lên: 12/09/2015, 08:19

... interphase microtubule arrays 4 .5. 3 Lack of Mia1p and Alp14p result in failure of iMTOCs establishment Another striking aspect of Mia1p function has been illuminated by lack of large interphase MTOCs in ... that S pombe cells overexpressing the TACC- related protein, Mia1p, were capable of organizing spindle-pole like structures While formation of intranucl...

Ngày tải lên: 12/09/2015, 08:19

... spindle assembly checkpoint in fission yeast Nat Cell Biol 5, 764 - 766 ; author reply 766 68 Sato, M., and Toda, T (2007) Alp7 /TACC is a crucial target in Ran-GTPasedependent spindle formation in ... Isolation and characterization of AINT: a novel ARNT interacting protein expressed during murine embryonic development Mech Dev 97, 13- 26 66 Samejima, I., Lourenco, P.C., Snait...

Ngày tải lên: 12/09/2015, 08:19

... INVESTIGATION OF THE ROLES OF TWO RAC1 EFFECTORS PHOSPHATIDYLINOSITOL- 5 KINASE Iα AND P 21- ACTIVATED KINASE IN INSULINSECRETING CELLS ZHANG JIPING (B.Sc., SICHUAN University) A THESIS SUBMITTED ... PIP5K-Iα in insulin secretion in INS -1 cells 11 1 4 .1. 3 Implication of PIP5K-Iα in PIP2 production in INS -1 cells 1 15 4 .1. 4 Role of...

Ngày tải lên: 14/09/2015, 14:08

... INTRODUCTION 1. 1 β-cell and insulin secretion 1. 1 .1 Diabetes mellitus 1. 1.2 Insulin 1. 1.3 Insulin secretion 1. 1.4 insulin- secreting cell model 1. 1.5 β-cell growth and cell cycle 1. 2 HAP1 10 1. 2 .1 HAP1 background ... of HAP1 in insulin- secreting cells after knockdown of its expression in INS -1 cell line The findings of HAP1 role in INS -1 cel...

Ngày tải lên: 12/10/2015, 17:35