characterization of the ferm domain protein ehm2 in human cancer cells

... acetylcholine receptor (Abcam), membrane protein from the cell lines were assessed by western blotting Expression of α7 protein was seen in of the SCLC cell lines (Figure 4) Expression of α7 protein ... nicotinic acetylcholine receptor, it was necessary to show if expression of the α7 nicotinic acetylcholine receptor protein in seen in these cell lines Using a specific antibody for the α7 nicotinic ... (normal cells) , the adenocarcinoma cell line A549 and the squamous cell line NCI-H226 express protein for α7, whereas the adenocarcinoma cell line NCIH322, the carcinoid cell line NCI-H727, the squamous...

Ngày tải lên: 12/08/2014, 18:21

... as they are product of sequence duplications Keeping in mind that the protease domain is part of a multi -domain protein, additional interactions among the domains may influence the folding of individual ... points of control of pararetroviruses http://www.virologyj.com/content/2/1/33 Figure In vitro releasing of the coat protein from the polyprotein P3 In vitro releasing of the coat protein from the ... cylinder and indicated as α Thick lines connecting the elements are loops and dashed lines indicate a break in the sequence The black triangle indicates the location of the active site after induction...

Ngày tải lên: 19/06/2014, 08:20

... as they are product of sequence duplications Keeping in mind that the protease domain is part of a multi -domain protein, additional interactions among the domains may influence the folding of individual ... points of control of pararetroviruses http://www.virologyj.com/content/2/1/33 Figure In vitro releasing of the coat protein from the polyprotein P3 In vitro releasing of the coat protein from the ... cylinder and indicated as α Thick lines connecting the elements are loops and dashed lines indicate a break in the sequence The black triangle indicates the location of the active site after induction...

Ngày tải lên: 20/06/2014, 04:20

... titration In the ts, the protein concentration was left free, to serve as an indicator of stoichiometry In the case of 1IR, the t resulted in a binding stoichiometry of : 1, i.e dimer binding The reactant ... rearrangement of the relative orientation of the two domains [7,9] The conformational change upon iteron binding may expose a recognition site for proteinprotein interaction, enabling coupling of recently ... quenching However, judging from the binding stoichiometry together with the shape of the binding curves (Fig 3), it is safe to conclude that the quenching does not affect the RepADNA binding equilibria,...

Ngày tải lên: 07/03/2014, 04:20

... to the right Conserved amino acid residues forming the active site of the A-chain are marked with asterisks [27] The residues participating in galactose binding in the 1a and 2c subdomains of the ... 55(70%) of identity (similarity) to the ricin B-chain, respectively The residues forming the sugar-binding sites in the ricin B-chain are generally conserved in the structure of the MLp B-chains: ... replacement of Trp38 fi Ser in the ML2p B-chain and Tyr252 fi Phe in the ML3p B-chain (Fig 2) The highly conserved amino acid residues forming the hydrophobic core of the ricin B-chain domains [28]...

Ngày tải lên: 07/03/2014, 15:20

... within A of Ile1126 in the AdoMet-binding region In the human structure, the longer a3 helix ends at the beginning of the extra five amino acids The 3102helix is oriented below the beginning of helix ... lm for the FMN domain, which is 10-fold lower than the apparent Kd (11.9 lm) for binding of the D963E ⁄ K1071N to the flavin-binding protein The presence of AdoMet in the fluorescence binding assays ... represents the change in fluorescence intensity, F0 is the measure of the fluorescence intensity in the absence of the activation domain, E0 is the concentration of the FMN domain, L denotes the concentration...

Ngày tải lên: 23/03/2014, 09:21

... 15-fold increase in the yield of the soluble protein Pantetheinylation of the ACP in ACPCON didomain was verified using MALDI-TOF of a tryptic fragment of the purified protein (data not shown) The ... was indeed only transferred to the active site phosphopantetheine thiol of the ACP domain The increase in labeling of the ACP-CON didomain may be a result of improved folding of the ACP domain ... that the individual domains of the megasynthase may remain folded despite the lack of the KS dimer Minimal polyketide synthase domains in LovB Characterization of the standalone MAT protein The...

Ngày tải lên: 30/03/2014, 08:20

... [7,11,20] GL is one of four glycogen-targeting proteins expressed in liver [21–25] These proteins have binding sites for protein phosphatase-1 (PP1) and for glycogen, and they differ in their relative ... but not R6, in rat liver in vivo is insulin-dependent It declines during insulin deficiency and is restored by insulin treatment [22,40] Another glycogen-targeting protein expressed in rat liver ... with insulin Diabetes 48, 15–20 39 de la Iglesia N, Mukhtar M, Seoane J, Guinovart JJ & Agius L (2001) The role of the regulatory protein of glucokinase in the glucose sensory mechanism of the...

Ngày tải lên: 30/03/2014, 11:20

... domain eliminated the ability of p67phox to activate the NBTreductase activity of the flavoprotein domain These data indicate that the target of the activation domain of p67phox resides within ... Expression and characterization of the flavoprotein domain of gp91phox Biberstine-Kinkade KJ, Yu L, Dinauer MC Mutagenesis of an arginine- and lysine-rich domain in the gp91(phox) subunit of the phagocyte ... within the flavoprotein domain of gp91phox These data provide a physical explanation for data indicating that the activation domain of p67phox controls the reduction of FAD by NADPH [32] Interestingly,...

Ngày tải lên: 07/08/2014, 14:22

... present in the tissues lacking TIP1;1 (Fig 2) It is therefore possible that these remaining isoforms compensate for the lack of TIP1;1 in the knockout On the other hand, the effect of the absence of ... 2-4 cells at the LRP As the LRP grows further, the number of cells expressing TIP2;1-YFP increases but remains confined to a cluster underlying the base the lateral root (Fig 4, panels F-I) In ... expression of TIP3;1 and TIP1;1 in Arabidopsis and found minimal overlap in the timing of their expression, with TIP3;1 being abundant in embryos of mature seeds and sharply declining during seed germination,...

Ngày tải lên: 12/08/2014, 03:21

... results in destabilization of the GTPase with the β-phosphate of the nucleotide [242, 246, 247] 1.3.1.2 The PH Domain The PH domain is invariably found on the C-terminus end of the DH domain, and these ... illustrate the biological importance of the PH domain and membrane targeting of the exchange factor by removing the PH domain of the exchange factor Lfc The transforming potential of Lfc in mouse ... phosphorylation of the RNA pol II Cterminus domain in a CDK7-dependent manner, thus inhibiting transcription [91, 92] The p21Cip1 family of inhibitors bind and block the activity of all the cyclin-CDK...

Ngày tải lên: 11/09/2015, 16:04

... ubiquitin attached to Protein X and the Cterminus to Protein Y and the reporter RUra3p 47 Figure 2.8 Interaction between Protein X and Protein Y Interaction of the two proteins brings about the ... presence of galactose triggered the protein- protein interaction of Gal3p with Gal80p in the cytoplasm, reducing the concentration of Gal80p in the nucleus This caused a reduction in the binding of ... transcription by initiating the recruitment of limiting factors at the promoter The raising of the local concentrations of these limiting factors at the promoter brings about the recruitment of other transcription...

Ngày tải lên: 12/09/2015, 08:20

... I Introduction - 13 - domain Other domains including the central putative coiled-coil domain, the pleckstrin homology (PH) domain, the GTPase effector domain (GED), and the COOH-terminal proline- ... PRD, proline arginine rich domain (B) The domain structure of dynamin-like proteins 1.3.1.1 Domains and properties of dynamins Dynamins are different from the small GTPases in that they are large, ... Camilli, 1997) These interactions are mediated by the SH3 domains of the accessory factors and the PRD domain of dynamins (Fig 1.7) Overexpression of the SH3 domain of these accessory proteins leads...

Ngày tải lên: 16/09/2015, 15:54

... chlorate, in inducing structural alteration of HS chains, prevents the bFGF binding Our results indicate that the presence of sodium chlorate abolishes the bFGF biological effects in developing Sertoli ... abolish sulfation on proteins and carbohydrate residues in intact cells without inhibiting cell growth or protein synthesis [35±37], and proteoglycan sulfation in cultured Sertoli cells from 20-day-old ... implication of cAMP increase, the second messenger of FSH, in the bFGF regulation was evaluated by addition to the culture medium of either mM dbcAMP or 10 lgámL)1 cholera toxin Their addition increased...

Ngày tải lên: 21/02/2014, 03:20

... found in various proteins This domain is a part of the nidogen glycoproteins, which are expressed by mesenchymal and epithelial cells Nidogens have a high affinity for lamininbinding protein and ... Exons Introns No Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human Mouse Human ... organization of MUC4 we determined by comparison of the published cDNA and the evolving human draft sequence [20] is very close to that of the mouse Muc4 (Table 1) The size of intron of the human MUC4...

Ngày tải lên: 08/03/2014, 23:20

... role for integrin alpha beta in the homing of monocytes to tumors Specifically, the group noted that blocking this integrin in a mouse model of implanted lung cancer suppressed the number of macrophages ... than 0.05 Injection of free DiD resulted in no increase in fluorescent intensity within the tumor at any time point post-infusion Figure labeling (a) Optical imaging of DiD-labeled cells immediately ... in the acknowledgements section and was involved in the study design HED-L was involved in the conception of the study and was the primary investigator on Award Number R21CA129725 listed in the...

Ngày tải lên: 18/06/2014, 15:20

... eIF4E binding protein beclin-1-regulated autophagy AMP activated protein kinase artesunate bafilomycin A1 Bax-interacting factor big mitogen-activated protein kinase bovine serum albumin cycloheximide ... regulatory-associated protein of mTOR rapamycin-insensitive companion of mTOR reactive oxygen species Beclin-1 interacting and cystein-rich containing S6 kinase Small interfering RNA soluble N-ethylmaleimide-sensitive ... RED (MTR) STAINING 55 2.9 MAGIC RED CATHEPSIN B AND L ACTIVITY ASSAY 55 2.10 DETERMINATION STAINING 56 2.11 IMMUNOFLUORESCENCE STAINING 56 OF PROTEIN PROTEOLYSIS USING viii DQ...

Ngày tải lên: 09/09/2015, 08:16

... human cancers, inferring biological role of TROM in promoting immuno-escape by cancer cells Moreover, we observed a strong correlation of TROM with the advancement of the disease in various cancers ... strand of the cDNA during second strand synthesis Section Introduction In brief, to tackle these problems, we use the concept of oligo(dT)-T7 priming, by incorporating the T7-promoter sequence to the ... dependent The disease is rare in most part of the world (0.7% of all cancer incidence), but highly prevalent among people in Southern China, Southeast Asia, Northeast India, North Africa and among the...

Ngày tải lên: 12/09/2015, 09:59

... the importance of PT in the mitochondrial apoptosis, because in the absence of the hallmarks of PT, including the loss of the inner membrane potential and the rupture of the outer membrane, the ... JAB Janus kinase binding protein JAKs Janus protein- tyrosine kinases JNK c-Jun N-terminal kinases MAPK mitogen activated protein kinase xiv MAPKK mitogen activated protein kinase kinase MAPKKK ... hypoxia), and they found that the type, strength and kinetics of the target gene profiles depend on the levels of p53 in the cell, the type of inducing agent, and the cell type investigated (Wang...

Ngày tải lên: 14/09/2015, 14:11

... efficacy of mTOR kinase domain inhibitors in preclinical and clinical studies (Menon and Manning 2008) These will be discussed in detail in the context of rapamycin‟s status in preclinical and clinical ... FK506-binding protein 12 (FKBP12) and directly binds to and inhibits TOR proteins through an allosteric site N-terminal to its kinase domain Called the FKBP12-rapamycin binding (FRB) domain, this ... at the G1 phase of the cell cycle in eukaryotic cells, ranging from yeast to human (Menon and Manning 2008) This, in part explains why rapamycin treatment in the clinic leads to cells in the...

Ngày tải lên: 05/10/2015, 13:53