Exploration of role of ncrlp, yeast homologue of NPC 1, in membrane trafficking

... and trafficking In this thesis we will study the NPC1 homologue in yeast, NCR1 Specially, we will examine the role of Ncr1p in membrane trafficking 1.2 Use yeast as a model to study membrane trafficking ... sorting) in ncr1-deleted yeast strain as well as in two vacuole protein sorting mutants vps20- and vta1- delete strains in order to test if the primordial...

Ngày tải lên: 05/10/2015, 22:15

... relevance In the study of White (2005), factors used for ranking sentences include the position of sentence in the source document, the words contained in sentence and the number of query terms contained ... important to sentence relevance How to incorporate this kind of information with information about dependences in 98 query to realize the most efficient sentence r...

Ngày tải lên: 08/03/2014, 02:21

... staining for phenotypic markers in chondrocyte-like cells from human intervertebral discs Immunohistochemical staining for collagen phenotypic markers in chondrocyte-like cells from human intervertebral ... Suda A: Cathepsin G in degenerating and healthy discal tissue Clin Exp Rheumatol 1999, 17:197-204 Gruber HE, Hanley EN Jr: Analysis of aging and degeneration of the hu...

Ngày tải lên: 09/08/2014, 06:22

... the role of HIF-1 in chondrocytic energy generation, matrix synthesis and cell survival During hypoxic and normoxic conditions, functional inactivation of HIF-1α in the presence of interleukin-1β ... the work by Yudoh and colleagues adds further experimental evidence for the suggested role of HIF-1 in protecting chondrocytes from cell death induced by pro-i...

Ngày tải lên: 09/08/2014, 07:20

... disruption of the STAT1 gene in mice revealed a role for STAT1 in the JAK-STAT signaling pathway [40] The JAK-STAT signaling pathway is involved in mediating biologic responses induced by many cytokines ... Post Infection Figure Replication of HSV-1 in macrophages Replication of HSV-1 in macrophages Analyses of virus replication, viral gB mRNA, and...

Ngày tải lên: 12/08/2014, 04:21

... disruption of the STAT1 gene in mice revealed a role for STAT1 in the JAK-STAT signaling pathway [40] The JAK-STAT signaling pathway is involved in mediating biologic responses induced by many cytokines ... Post Infection Figure Replication of HSV-1 in macrophages Replication of HSV-1 in macrophages Analyses of virus replication, viral gB mRNA, and...

Ngày tải lên: 12/08/2014, 04:21

... demonstrate the presence of such opposite changes in the responsiveness of the airway and tissue compartments in the presence of oxygen toxicity Indeed, expression of the hyperoxia-induced changes in the ... in larger mammals [9,45] and in mice [46] Conclusion In summary, the results of the present study yield evidence of the involvement of ET-1...

Ngày tải lên: 12/08/2014, 16:20

... injury by inducing expression of cytokines including tumor necrosis factor and IL-6 and IL-8 [149] These cytokines may in turn stimulate the production of many inflammatory mediators, leading to lung ... synthase in the lung [148] ARDS is also characterized by the presence of inflammatory cells in the lung Since ET-1 may act as an immune modulator, an increase in ET-1 may...

Ngày tải lên: 12/08/2014, 18:20

... 11 3 6 .1. 4 Mutagenesis 11 3 6 .1. 5 6.2 HCT 116 and E1 cell lines 11 2 Transfection 11 4 Cell-based assays 11 5 6.2 .1 6.2.2 Wound healing 11 5 6.2.3 ... Stathmin- 1 1. 2 .1 The Stathmin family 1. 2.2 STMN1 in microtubule regulation 10 1. 2.3 STMN1 in cell cycle regulation 12 1. 2.4 STMN1 in cancer 14 ... Conclusion and future work 10 7 Chapter Materials...

Ngày tải lên: 09/09/2015, 11:25

... 11 6 11 9 4 .1 The role of Annexin- 1 in the regulation of inflammatory stress 11 9 response 4.2 Conclusion 14 4 4.3 Limitations of the study 14 5 4.4 Future work 14 6 Bibliography 14 8 Summary Annexin- 1 ... for investigation of the basis of the inflammatory stress response The aims of this study are thus, 1) To determine the role...

Ngày tải lên: 01/10/2015, 17:27

... function upstream of the effector caspases in apoptosis and are known as initiator caspases A phylogenetically distinct group of 13 caspases (caspase- 1, caspase- 4, caspase- 5, caspase- 11 and caspase- 12 ) ... caspase- 1 to an influenza A/ Puerto Rico/8/34 (H1N1) virus infection of RAW264.7 murine macrophages, in vitro To investigate the role of ca...

Ngày tải lên: 13/10/2015, 16:41

... junction barrier integrity (7) Conversely, activation of atypical PKC isoforms using bryostatin instead increased the expression of claudin-1 in the tight junction complex, and increased tight ... cell motility We have previously shown that increasing the levels of CLDN-1 increases the invasion of melanoma cells (4) To determine if the increase in claudin-1 needs...

Ngày tải lên: 03/11/2012, 11:17

... protein Kinesin light chain Kinesin heavy chain Microtubules Figure Schematic diagram of a kinesin- cargo complex The cargo vesicles are thought to be associated with kinesins through the interactions ... the defects of Drosophila kinesin mutants in axonal transport, suggesting that UNC-76 coordinates with kinesin to regulate cargo trafficking in axons [23] The similarity...

Ngày tải lên: 06/08/2014, 18:21

... repeats (ANK), a transactivation domain (TAD) and a PEST domain (P) TM, transmembrane domain (b,c) Two models for the roles of Ofut1 in Notch trafficking Each model is illustrated in a schematic ... (green) adapted from [19] The S2 cleavage site is indicated by an arrow The intracellular domain (NICD) contains various elements involved in transcriptional activation:...

Ngày tải lên: 06/08/2014, 18:21

... demonstrating an increase in the level of caveolin-1 phosphorylation in irradiated rats located primarily in the microglial and vascular endothelial cells of the spinal cord The findings suggest that the ... activation in the microglia are associated with the phosphorylation of caveolin-1 in the spinal cord of irradiated rats, leading to...

Ngày tải lên: 07/08/2014, 20:23