Functional studies of BPGAP1, a novel BCH domain containing

Functional studies of BPGAP1, a novel BCH domain containing RhoGAP protein

... and ARAP3 (Krugmann et al., 2002; Miura et al., 2002) All these three ARAP contain five PH domains, an ArfGAP domain and a RhoGAP domain (Miura et al., 2002) ARAP1 and ARAP3 have equal GAP activity ... Sekimata et al., 1999) PSGAP, a protein that interacts with PYK2 and FAD and contains multiple domains including a pleckstrin homology (PH) domain, a RhoGAP- activating prote...

Ngày tải lên : 17/09/2015, 17:20

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Structural and functional studies of VP9, a novel nonstructural protein from white spot syndrome virus

... Dr Asha, Dr Huang Canhua, Dr Wu Jinlu, Ms Tang Xuhua, Ms Sunita and, Mr Jobi and the rest of the lab mates for the valuable discussion and friendship and the present and former members of Functional ... Birnaviridae, Bunyaviridae, Herpesviridae, Piconaviridae, Parvoviridae, Reoviridae, Rhabdoviridae, Togaviridae, Iridoviridae, Nodaviridae and Nimaviridae Crustacean viral dise...

Ngày tải lên : 14/09/2015, 09:57

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Structural and functional characterization of haditoxin, a novel neurotoxin isolated from the venom of ophiohagus hannah (king cobra

... β-cardiotoxin, an all β-sheet protein isolated from the venom of Ophiophagus hannah (king cobra) Manuscript under preparation xvi (5) Roy A, Sivaraman J, and Kini RM Structural and functional characterization ... forests and mangrove swamps in parts of Southeast Asia, South China and India Chapter One A B C Figure 1.1: Ophiophagus hannah (king cobra)...

Ngày tải lên : 10/09/2015, 08:37

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Functional characterization of isthmin, a novel secreted protein in angiogenesis

... proteins, protein kinases and phosphatases Actin binding proteins that localize at FA sites include talin, tensin, paxillin, vinculin and α-actinin (Yam, et al., 2009) The assembly of FAs can be induced ... and adaptor proteins in focal adhesion complexes There are 25 four main signaling pathways activated by integrins that are relevant to angiogenesis: Ras-mitogen-activated protei...

Ngày tải lên : 14/09/2015, 08:25

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Functional analysis of syp1, a novel substrate of the serine threonine kinase prk1

... 40 The homologous domains with Syp1p through searching against database - 89 x Abbreviations a. a or aa amino acid AAK1 adaptor-associated kinase ADF actin depolymerizing factor ADFH actin ... FUNCTIONAL ANALYSIS OF SYP1, A NOVEL SUBSTRATE OF THE SERINE/ THREONINE KINASE PRK1 QIU WENJIE A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE...

Ngày tải lên : 14/09/2015, 11:59

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A novel sec7 domain containing protein BIG3 and its role in regulated secretory pathway

... 5’-GAG AAG AAG GAT CCC AGC CGG AAG AAG-3’ and 5’-CGC GTC GAC CAC AAT GAT GTC ATA GAC-3’ and digested with BamHI-SalI and ligated to C-terminal of EcoRI-BamHI fragment in pUC19 The full length of BIG3 ... The interaction of TGN subdomain and motor is selective It can be a direct binding of a cargo protein with a motor protein dynein (Tai et al., 1999); or mediated via ada...

Ngày tải lên : 11/09/2015, 09:15

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Functional studies of a type III and a novel secretion system of edwardsiella tarda

... such as Escherichia coli, Salmonella, Shigella, and Yersinia species E tarda is a relatively new genus, and the first report about the genus of Edwardsiella was in Japan by Sakazaki and Murata (1962) ... bromo-4-chloro-3-indolyl-β-D-galactopyranoside xv SUMMARY Edwardsiella tarda is an opportunistic gram-negative bacterial pathogen affecting both animals and humans The abi...

Ngày tải lên : 15/09/2015, 17:11

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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... S103 G145/D137 Y 112 D2 01/ 193 L75/67 G145/D137 E1 41/ 133 K173 /16 5 R207 /19 9 G77/69 D142 /13 4 G197 /18 9 Fig Superposition of PRTFDC1 with HPRT-ImmGP (Protein Data Bank code: 1BZY) (A) Residues in the ... Welin et al Studies of the human PRTFDC1 B A PRTFDC1 25 HPRT 20 10 00 0.2 800 600 400 0 .1 200 –50 50 10 10 50 10 015 0 200 250 S 200 –50 250 50 12 00 10 0 15 0 [H...

Ngày tải lên : 15/02/2014, 01:20

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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... has already established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat ... (Fig 4A and B, 5) The lipase activity of the K413A mutant is similar to that of wild type LPL, whereas the esterase activity is reduced to 60% of t...

Ngày tải lên : 21/02/2014, 15:20

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Báo cáo Y học: Functional studies of the Synechocystis phycobilisomes organization by high performance liquid chromatography on line with a mass spectrometer docx

... and phycocyanins, HPLC separation allowed a quantitative estimation of the relative amount of each protein component from the area underlying each peak This was facilitated by the fact that the ... region rich in amino acids with a higher than average contribution to the UV optical absorption and protonation of amino groups by electrospray ionization, leading to fals...

Ngày tải lên : 08/03/2014, 16:20

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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... mechanism of both RNA and DNA synthesis [17] In a previous study, we reported the ﬁndings of an analysis of the complete sequence of the cryptic plasmid pIT3 isolated from the crenarchaeon S solfataricus ... analyses of the predicted amino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the heli...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... catalytic residues: E52 and R105 It is evident from the kinetic results that mutation of E52 changes only the catalytic rate For the E52D mutant the Km value was approximately the same as for the wild-type ... mutation of deoxyribonucleoside kinase L Egeblad-Welin et al Human deoxyribonucleoside kinases are targets for the chemotherapeutic treatment of...

Ngày tải lên : 19/02/2014, 02:20

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Báo cáo khoa học: Enhanced peptide secretion by gene disruption of CYM1, a novel protease in Saccharomyces cerevisiae doc

... LJY430 LJY431 LJY432 MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa MATa Euroscarf [16] ATCC [15] This study ... the yeast deubiquitinating enzyme Ubp16, a metalloprotease belonging to the large family of deubiquitinating enzymes, mainly consisting of cysteine proteases [43] Another class of p...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo khoa học: Relation between domain evolution, speciﬁcity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot

... involve also the cluster of acarviose transferase (Actsp), archaeal CGTase (Thcsp) and the maltogenic a- amylase (Bacst) due to its longer branch separating it from the Ó FEBS 2003 a- Amylase family members ... branches adjacent to each other and close to the border that separates the two major parts of the tree Note that the B stearothermophilus maltogenic a...

Ngày tải lên : 08/03/2014, 08:20

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Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot

... Hemolymph titers of PO -CHH and ES -CHH in response to changes in dissolved oxygen Hypoxia induced the release of CHHs from the PO and ES of the juvenile crabs (Fig 6) At the initial control normoxic ... of CHH cDNA from the ES [28] Also, we examined the physiological responses of the release and expression of these two CHHs under stressful co...

Ngày tải lên : 16/03/2014, 06:20

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