transmitochondrial cybrids tools for functional studies of mutant mitochondria

... site mutation of deoxyribonucleoside kinase Table Kinetics of dThd and dCyd phosphorylation for active-site mutants of Dm-dNK AraC, AraT and BVDU were tested only with the Y70W mutant kcat values ... Kirschbaum B & Loffler M (1996) Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified ... site mutation of deoxyribonucleoside kinase L Egeblad-Welin et al Mutants of substrate-binding residues: Y70 and Q81 Y70W showed an increase in Km values for both dThd and dCyd, of approximately...

Ngày tải lên: 19/02/2014, 02:20

... addition of PRPP has been observed to induce tetramerization for HPRTs [21,22] and the use of 10 mm PRPP in crystallization set-up for PRTFDC1 could explain the different oligomeric states for PRTFDC1 ... was run for HPRT but, unfortunately HPRT gave uninterruptable aggregation temperature profiles Comparison with HPRTs Superposition of PRTFDC1 and human HPRT results ˚ in a rmsd of 1.0–1.7 A for approximately ... six-fold higher for PRTFDC1, and Vmax is approximately 70-fold lower compared to those of HPRT Thus, the kcat ⁄ Km value with PRTFDC1 is only 0.26% of that of HPRT The Km (guanine) value for PRTFDC1...

Ngày tải lên: 15/02/2014, 01:20

... to form functional channels in any pairwise combination [13–15] From analysis of single channel conductances obtained upon coinjection of wild-type and mutant subunits, and from quantification of ... range of activities have been identified Although the less selective peptides might be less useful as pharmacological tools, they provide information for structure-activity studies Comparison of ... Crystal structure of participate in the formation of EpI-resistant a7 receptors, or an ACh-binding protein reveals the ligand-binding domain of that the functional properties of the nAChR are...

Ngày tải lên: 19/02/2014, 12:20

... of hydrogen, modification of bonds, potentials of forcefield, and fixation of heavy atom, backbone, and Ca, were performed for molecular mechanics calculations and then the energy minimization of ... structures of the human and porcine pancreatic lipases for the open form and on the horse pancreatic lipase for the closed form [16,18,19,30] For homology modeling, the 11 C-terminal residues of LPL ... Genome Center of IMS, University of Tokyo) for computer system support This work was supported in part by a Research for the Future Program Grant of The Japan Society for the Promotion of Science...

Ngày tải lên: 21/02/2014, 15:20

... typical positive ion spectrum of a single protein consists of a series of peaks, each of which represents a multiply charged ion of the intact Injection of the total mixture of phycobilisomes from Synechocystis ... having a specific number of protons attached to the basic sites of the amino-acid sequence The m/z values for the ions have the general form [M + zH]/z, where z equals the number of protons attached ... efficient separation of phycobilisomes upon injection of entire subcomplexes [14] The nondisruptive nature of this method has made it possible to collect information on the composition of each subcomplex...

Ngày tải lên: 08/03/2014, 16:20

... and 72 °C for min; three cycles at 94 °C for 30 s, 50 °C for 30 s, and 72 °C for min; 24 cycles at 94 °C for 30 s, 55 °C for 30 s, and 72 °C for min, and a final extension at 72 °C for A nested ... Conditions of TD-PCR were as follows: initial denaturation at 94 °C for min; three cycles at 94 °C for 30 s, 54 °C for 30 s, and 72 °C for min; three cycles at 94 °C for 30 s, 52 °C for 30 s, ... used for cloning of 5¢-3¢-RACE of PO-CHH LF and LR primers were used for standard cRNA production for quantitative RT-PCR, whereas SF and SR primers were for quantitative RT-PCR A combination of...

Ngày tải lên: 16/03/2014, 06:20

... presence of trimers in solution All determinations of native molecular masses reported here were performed with excess of Mg2+ in solution to allow the interactions involved in hexamer formation ... molecular mass of the detected protein band, as is also the case for metal cation dependence, as the sPPase activity of many of these bacteria can efficiently use other divalent cations as cofactors, ... genomes of a number of prokaryotes from diverse taxonomic groups with functional family II sPPase genes, thus illustrating functional substitution by nonhomologous sPPases in a context of gene...

Ngày tải lên: 16/03/2014, 11:20

... two factors : the dispersal of information and the obscurity of the coding In ARIANE-78, the lexical data base may reside on much more 50 files, for a given pair of language Figure Screen Display ... translator This data base is composed of dictionaries, "formats" and "procedures" of the analysis, transfer and synthesis phases (the conventional phases of a CAT system) For any given source lexical ... any given source lexical unit in this data base, VISULEX searches for all the associated information THAM consists of a set of functions programmed in the macro language associated with a powerful...

Ngày tải lên: 17/03/2014, 19:21

... approaches offer generic advantages for the analysis of protein complexes both in terms of dynamic range (i.e how little of one protein can be identified in the presence of how much of a mixture of other ... expression of downstream target genes of the Wnt-signaling pathway [9–11] Both interactions are largely independent of each other and knowledge of both yields information on different aspects of beta-catenin ... high number of peptides of the complete protein that would be required for an unambiguous identification Clustering of ESTs and gene prediction can make this information available for searching...

Ngày tải lên: 31/03/2014, 07:20

... mobility of Aβ peptides, including dynamics of their self-assembly Lipidomics tools are now available for detailed studies of molecular lipids in cells and biofluids [48] Molecular profiling, ... encompass only informatics and statistics; for example, it includes the development of tools not only for storing and mining the data, but also modeling of the data in the context of disease pathophysiology ... therapeutic avenues Bioinformatics tools enabling a systems medicine approach to AD Many tools are available for mining of heterogeneous biological data, although the focus of such tools and the challenges...

Ngày tải lên: 11/08/2014, 12:21

... pattern of developmental changes, by considering systems of differential equations for biological clocks Simulation studies have been performed to investigate the statistical properties of the ... respectively For a particular genotype j of this QTL (j = for aa, for Aa and for AA), the parameters describing circadian rhythms are denoted by Θuj = (nj, mj, τj, rMj, rPj, qMj, qPj, kj) Comparisons of ... (Σ) of phenotypic values for traits y and z can be structured in terms of φy, φz, ψy, ψz and Σε(t) by a bivariate SAD(1) model [15,16] Also, the closed forms for the determinant and inverse of...

Ngày tải lên: 13/08/2014, 16:21

... versus nario heregenes for high-fatfor the simulation GO andlists Results for dataand plots) for additionalII, GOcorrelated under Additionalforsets ofthe VILI example with forwithof three lists scegenes ... associated (the number of simulated genes in common is equal to 0), the plot of T(q) for different cut-offs q is, as expected, a horizontal line of height 1, with evidence of noise for small p values ... set of values of q for which CI95(q) excludes 1} and q2 = max{over the set of values of q for which CI95(q) excludes and Median(R(q) | O, n) ≥ 2} We averaged the results over 50 repeats for each...

Ngày tải lên: 14/08/2014, 20:22

... directly from data SISSIz offers some heuristics for estimating parameters, without proof of the convergence or accuracy of these estimates EvolSimulator does combine evolution of gene families and ... cost of also missing all the real genes in the dataset To get a true picture of the predictor's performance, one must therefore consider how the number of false positives varies as a function of ... collected for the first time in a single place Many of these model-based simulation tools share a fundamental deficiency in their treatment of missing parameters and data Specifically, they often...

Ngày tải lên: 14/08/2014, 21:20

Ngày tải lên: 14/11/2014, 08:21

... produce a large amount of samples for X-ray, NMR and functional studies These experiments will be the key to a deeper understanding of the biology of WSSV, in terms of mechanisms of host-pathogen protein-protein ... monitor the production of amplified products during each cycle of the PCR reaction It is one of the technologies of the genomic age and has become the method of choice for detection of gene expression ... information Figure 3.2 Hydrophobicity plot of VP9 46 Figure 3.3 Purification profile of His-VP9 47 Figure 3.4 MS result of native VP9 48 Figure 3.5 DLS result of VP9 49 Figure 3.6 CD result of...

Ngày tải lên: 14/09/2015, 09:57

... Prof S J Busby, Prof C W Stephan for providing plasmids for my research work Sincere thanks to Prof Ilan Rosenshine for his generous sharing of ideas and experiences I also wish to thank Prof ... FUNCTIONAL STUDIES OF A TYPE III AND A NOVEL SECRETION SYSTEM IN EDWARDSIELLA TARDA ZHENG JUN (B.Sc, M.Sc) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... Proteome analysis of E tarda PPD130/91 and the ΔesrC mutant 64 Fig III.4 Transcription of eseD was reduced by the mutation of esrC .65 Fig III.5 Effect of loss -of- function mutants in regulatory...

Ngày tải lên: 15/09/2015, 17:11

... _ identified, some with multiple isoforms They inculde: Rho(A,B,C isoforms), Rac (1,2,3 isoforms), Cdc42 (Cdc42Hs, G25K isoforms), Rnd1/Rho6, Rnd2/Rho7, Rnd3/RhoE, RhoD, RhoG, ... representation of selected human RhoGAP domain-containing preoteins Domain organization of Cdc42GAP-like proteins Molecular cloning of different isoforms of BPGAP family cDNA and protein sequences of BPGAP1 ... FUNCTIONAL STUDIES OF BPGAP1, A NOVEL BCH DOMAIN-CONTAINING RHOGAP PROTEIN SHANG XUN (M.Sc., B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL...

Ngày tải lên: 17/09/2015, 17:20

... DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY ZHANG LEI B.SC (HONS.), NUS A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE GRADUATE ... Table 3.3: Summary of the data format of the shifts file 39 v List of Figures Figure 1.1: Effects of RF pulses on the net magnetization Figure 1.2: Fourier transformation of the FID Figure 1.3: ... of a protein is of great importance for the detailed understanding of its biological function At the present time, there are two main techniques that are capable of solving the 3D structure of...

Ngày tải lên: 04/10/2015, 15:45

... protein functional behavior [12] The recent development of functional protein microarrays, on which thousands of discrete proteins are printed at high spatial density, offers a novel tool for using ... collections of ASRs cover only a very limited fraction of the proteome and are unlikely to be available for most metabolites or drugs Labeling query molecules for large scale studies can often be ... magnitude of the change in the local index of refraction caused by binding is a function of the mass and conformation of both the query molecule and the immobilized protein, the number of query...

Ngày tải lên: 23/03/2014, 15:21

... reduction of energy requirements for heating is 11.3% for Athens and 6.1% for Thessaloniki On the contrary, for the cooling period of the 1993-2002 decade, an increase of the energy demands of the ... performance of buildings – Calculation of energy Use for space heating and cooling, International Organization for Standardization, 2008 [2] ASHRAE Handbook of Fundamentals, American Society of ... calculations were performed for all the winter and summer months for the two cities, and the energy requirements of the building were calculated for heating and cooling with temperature data of the decade...

Ngày tải lên: 05/09/2013, 16:10