The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders

... in the body There are four classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the ... stem cells versus embryonic stem cells The general differences between the characteristics of adult and embryonic stem cells are summarised in Table...

Ngày tải lên: 16/09/2015, 15:55

... for the HS4 core enhancer (HS4), a region 5¢- to HS3 (5¢HS3), the core of HS3 (HS3), a region flanking HS2 and HS3 (3 ⁄ flank), the core of HS2 (HS2), a region downstream of HS2 (3 HS2), and the ... progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133 + bone m...

Ngày tải lên: 07/03/2014, 12:20

... and cellular responses; and analysis of T-cell proliferation LG contributed to CIA induction and evaluation and to analysis of T-cell proliferation PM contributed to the design of the study and ... inhibition of T-cell proliferation was demonstrated by the addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respectively - to t...

Ngày tải lên: 12/08/2014, 11:23

... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potenti...

Ngày tải lên: 09/09/2015, 18:56

... transplantation of adult- derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have been isolated and characterized in vitro, from various regions of the adult ... plasticity of the nervous system Adult- derived neural progenitor and stem cells grafted in the brain promote neuroprotection, by an immunomodulatory...

Ngày tải lên: 08/08/2014, 17:20

... Expression of genes in the BMP2+ cells associated with plasticity, and mesodermal and NCSC phenotypes BMP2+ cells are still in a state of plasticity BMP2+ cells significantly upregulate Oct4 and Nanog ... 50 µm 20 µm Analysis neural crest stem cell associated transcripts in BMP2+ cells Figure of Analysis of neural crest stem cell associated transcripts in...

Ngày tải lên: 14/08/2014, 08:20

... Optimisation of the Oct4 and Sox2 ChIP assays 57 3.2.2 Oct4 and Sox2 bind to the distal enhancer of Oct4 in mESCs 58 3.2.3 Oct4 and Sox2 bind to the SRR2 of Sox2 in mESCs 59 3.2.4 Oct4 and Sox2 bind to ... Specificity of Oct4 and Sox2 antibodies used in ChIP 3.2 Oct4 and Sox2 binding to Oct4 CR4 region in mESCs 3.3 Oct4 and Sox2 bi...

Ngày tải lên: 11/09/2015, 16:03

... 35 04 - 36 02 139 - 22 6 20 7 - 31 3 45 0 - 559 11 13 - 130 8 1 637 - 1 8 32 1559 - 1665 39 9 - 5 92 3. 60 3. 62 3. 99 4 . 34 4. 78 3. 68 3. 26 3. 4 3. 4 32 .8 29 .25 30 .7 30 .8 35 .8 27 .2 29.1 28 .3 30.8 Bold designates ... Sox- 2 297 – 37 8 5 73 – 677 1056 - 11 53 71 - 23 0 11 82 - 1 34 5 4. 55 2. 98 3. 68 3. 49 4 .36 19 .4 33 .9 28 .2...

Ngày tải lên: 27/11/2015, 11:25

... 2675 Actin and ABPs in transcription regulation B Zheng et al Table Role of nuclear actin- binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain ... STARS-interacting proteins These novel proteins contain four LIM domains and a C-terminal villin headpiece domain, which mediates actin- binding in several proteins,...

Ngày tải lên: 07/03/2014, 01:20

... E.P., Martin-Eauclaire, M.F., Mansuelle, P & Sampieri, F (1991) An anti-insect toxin purified from the scorpion Androctonus australis Hector also acts on the alpha- and beta-sites of the mammalian ... Sampieri, F & Granier, C (1991) An anti-insect toxin purified from the scorpion Androctonus australis Hector also acts on the a- and b-sites of the mammalian s...

Ngày tải lên: 31/03/2014, 09:20

... family, the miR-200 family have been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining ... instance, miR-373 induces the expression of E-cadherin and CSDC2 by targeting their promoter region and initiate their expression[ 73] Another me...

Ngày tải lên: 18/06/2014, 15:20

... mechanisms involved in the increase or maintenance of bone mass in OA This study of the IT region of the proximal femur in primary hip OA and non-OA postmortem controls investigated changes in gene expression ... of combining gene expression analysis with histomorphometry allows the exploration of any relationships between gene expression and...

Ngày tải lên: 09/08/2014, 08:23

... the strains Gene expression differences of genes located in the NOD loci Idd5 and Nss1 The main focus of the gene expression profiling has been on identifying genes in the two NOD derived congenic ... that the loci affect the gene expression profiles in the SGs of the congenic mice The gene expression reflected the congenic f...

Ngày tải lên: 09/08/2014, 10:21

... targeted by miRNA (y- axis) as the percentage of disorder increases in the proteome The datasets for the transcripts encoding the disordered proteins (burgundy) and ordered proteins (mauve) and the proteome ... highly disordered proteins with the highly ordered proteins, an increase of 23.5% is observed instead of 42.5%, and comparing the disord...

Ngày tải lên: 14/08/2014, 21:20

... Using Biological Networks and Gene- Expression Profiles for the Analysis of Diseases LIM JUNLIANG KEVIN (B.Comp (Hons.), NUS) A DOCTORAL THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... some of these problems by incorporating biological information into their framework in the form of gene sets These gene sets represent biological processes...

Ngày tải lên: 09/09/2015, 10:15