control of prostate cancer proliferation and gene expression using herbal supplements

... induces genetic and epigenetic instability and phenotype changes in prostate cancer cells BMC Biol 2010, 8:6 24 Diaw L, Woodson K, Gillespie JW: Prostate cancer epigenetics: a review on gene regulation ... (GSTP1) gene Epimethylation of the GSTP1 gene is absent in normal prostate tissue and present in 6.4% of proliferative inflammatory atrophy, which is the precursor lesion of prostate cancer GSTP1 ... tumor invasion and metastasis, is decreased or absent in many cancers and is predictive of tumor progression and poor patient outcome In prostate cancer, decreased expression of E-cadherin correlates...

Ngày tải lên: 10/08/2014, 23:20

... other metabolic events in prostate cancer [14] Taken together, a combination of gene amplification and androgen stimulation may cause overexpression of TPD52 in prostate cancer TPD52 undergoes post-translational ... protein signature of prostate cancer biopsies revealed the overexpression of TPD52 in cancer patient material [22] To date, the main physiological role of TPD52 in prostate cancer progression ... cells [34,35] The expression of murine TPD52 in NIH3T3 cells induces the expression of several genes involved in the promotion of metastasis and the genes responsible for prevention of metastasis...

Ngày tải lên: 30/03/2014, 02:20

... copy number changes and gene expression profiles in a cohort of AA and CA PCa patients using BAC-based array comparative genomic hybridization (aCGH), oligo-based aCGH, and gene expression array ... reported as common areas of genomic gain in prostate cancer In one series of 18 prostate cancer cell lines and xenografts, 39% of samples had copy number gain at 5p15.33 and 39% had gains at 16p12.2-p11.2[29] ... finding of copy number gains at 16p11.2 and overexpression of RABEP2 in this previous study of PCa cell lines and in our current study of human PCa tissues is reassuring of the validity of the...

Ngày tải lên: 18/06/2014, 16:20

... localizes in the nucleus and binds to and regulates basal expression via HSEs of target genes [26] Deletion of the HSF gene is lethal to cells, even at physiological temperatures The genes targeted by ... levels of HSF1 and MTA1 (a component of the NuRD complex containing HDAC1 and HDAC2) proteins and to binding of HSF1 to MTA1 [78] The complex is recruited to the promoters of estrogen-responsive genes ... Sakurai and Y Enoki SWI ⁄ SNF complexes are recruited to both the promoter and coding regions, and histones are acetylated and displaced [56–58] However, the presence of SAGA and SWI ⁄ SNF, and a...

Ngày tải lên: 18/02/2014, 04:20

... 36.6 We next determined gene expression in different adipose depots in response to PM2.5 exposure, in terms of the expression of BAT-specific and WAT-specific gene profiles by real-time PCR analysis ... differences in the gene expressions of Hoxc9 or Igfbp3 in the WAT, neither was the gene expression of Dpt in BAT or WAT Xu et al Particle and Fibre Toxicology 2011, 8:20 http://www.particleandfibretoxicology.com/content/8/1/20 ... targeted disruption of the Dio2 gene have cold-induced overexpression of the uncoupling protein gene but fail to increase brown adipose tissue lipogenesis and adaptive thermogenesis Diabetes 2004,...

Ngày tải lên: 06/03/2014, 19:20

... MEF2 Second, overexpression of a-actinin-4 decreases the interaction of MEF2A and HDAC7 Third, knockdown of a-actinin-4 decreases expression of TAF55 Fourth, MEF2C, a-actinin-4 and HDAC7 associate ... inhibition of ARA54-enhanced androgen receptor transactivation and prostate cancer cell growth Mol Endocrinol 21, 343–358 103 Lee YH & Stallcup MR (2006) Interplay of Fli-I and FLAP1 for regulation of ... activities of myocardin and MRTF-A and -B, can completely block the ability of STARS to induce SRF-dependent transcription in NIH 3T3, COS1 and 293T cells However, STARS does not alter the level of expression...

Ngày tải lên: 07/03/2014, 01:20

Ngày tải lên: 01/04/2014, 13:20

... differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, the expression levels of let-7b and let-7i ... miRNA expression Gene expression profiling We assessed global hES gene expression profiles on separate passages of cells from different hES cell lines, EB samples at different time points, and ... gene pairs Correlation coefficients of miRNA-target gene pairs The expression of miR-302c and miR-502b and their predicted target genes was analyzed by correlation analysis The distribution of...

Ngày tải lên: 18/06/2014, 15:20

... Previously, analysis of NS3/4A-mediated inhibition of IFN gene expression has been restricted to IFN-β gene To further analyze whether the expression of other type I IFN Page of 13 (page number ... n ei pr ot co re F pc DN A3 Figure HCV NS2 and NS3/4A inhibit IFN-β gene expression HCV NS2 and NS3/4A inhibit IFN-β gene expression (A) The effect of expressed 11 HCV polypeptides on IFN-β promoter ... similar mechanism to inhibit IFN gene expression Cells were transfected with Flag-Cardif and increasing amounts of HCV NS2, NS3/4A and NS3/4A-S139A expression con- Page of 13 (page number not for citation...

Ngày tải lên: 20/06/2014, 01:20

... development of manifestations of lupus General aspects of Ig variable region (IgV) gene usage Autoantibodies are features of most systemic autoimmune diseases, including pSS The production and persistence ... distribution of B cells and their productive IgV gene rearrangements can be influenced by a variety of selective events during development and subsequent antigenic stimulation because of the nature of ... than other regions of the VH molecule Evidence of clonal expansions The major salivary glands are known to be the site of preferential B cell expansions and in some cases of lymphoproliferation in...

Ngày tải lên: 09/08/2014, 01:21

... et al.: Expression profiles of non-small cell lung cancers on cDNA microarrays: Identification of genes for prediction of lymphnode metastasis and snsitivity to anti -cancer drugs Oncogene 2003, ... induce these genes in group II The results of the study suggest that a combination of histopathology and gene- expression profiling is a useful tool for diagnostic and prognostic studies of early ... histopathogensis of synovitis in early RA Different expression profiles of the several candidate genes may provide useful information for future studies of the diagnosis and prognosis of early RA...

Ngày tải lên: 09/08/2014, 06:23

... http://www.ro-journal.com/content/5/1/108 Page of 12 Figure Anti-neoplastic effects of ErPC3 and ionizing radiation on prostate cancer cells The prostate cancer cell lines PC3, DU145, and LNCaP, were irradiated ... down-regulation of p-Akt may be required for the anti-neoplastic action of small molecule inhibitors of the PI3K/Akt pathway in prostate cancer cells Combined effects of ErPC3 and ionizing radiation in prostate ... to the design of the study VJ performed conception and design of the study and substantially contributed to interpretation of data, drafting and critical revision of the manuscript and final approval...

Ngày tải lên: 09/08/2014, 09:20

... standard deviation >0.7) In comparing the gene expression of the congenic strains with that of the control strains, the standard analysis elicited gene lists of 2,435 differentially expressed genes ... PCR validation of gene expression data Genes were selected from the gene lists of the standard and alternative analyses in order to verify the microarray results using QPCR Only genes with FDR ... data are robust and provide clear differences between the strains Gene expression differences of genes located in the NOD loci Idd5 and Nss1 The main focus of the gene expression profiling has been...

Ngày tải lên: 09/08/2014, 10:21

... of P63 + cancer cells in the metastatic loci and generation of prostate- like glandular structures Our data support the prostate cancer stem cell characteristics observed in previous studies and, ... loss -of- function mutations has been reported in some androgen-refractory prostate cancers [13,41] Therefore, both AR + and AR - cells were capable of forming androgen-independent prostate cancer ... Bonkhoff H: Role of the basal cells in premalignant changes of the human prostate: a stem cell concept for the development of prostate cancer Eur Urol 1996, 30:201-205 Collins AT, Maitland NJ: Prostate...

Ngày tải lên: 10/08/2014, 05:21

... List of primers used for real-time qRT-PCR of shoot and berry gene expression Primers were generated for realtime qRT-PCR of genes for comparison with microarray and EST frequency results Gene ... been generated and analyzed to identify gene expression related to a wide range of processes including berry development in wine grape [30,45] and in table grape [46], tissue-specific gene expression ... number of times the transcript of gene xi is observed in relation to the total number of random observations of all genes, (xi / ∑x), any ESTs from a single clone sequenced from both the 5’ and...

Ngày tải lên: 11/08/2014, 11:20

... identification and characterisation of genes is difficult EST generation supplements the lack of genome data by providing transcript specific information and excluding non-coding regions of the genome ... Quantitative gene expression analysis Expression analyses of nine genes were conducted at 0, 3, 6, 12, 24 and 48 hours post infection (hpi) to validate if the pyrosequencing data reflected their gene expression ... pyrosequencing data and gene expression profiles of defence-related genes Sequence ID GenBank accession number cDNA Library Max qRT-PCR expression Similarities between 454 and qRTPCR Thaumatin...

Ngày tải lên: 11/08/2014, 11:21

... see Ref [25]) The genetic selection and breeding activities of a few commercial conifer species are being expanded to include genetic mapping and marker development Candidate gene approaches are ... clues to the role of PgMYB11 in conifers The expression profiles of a few of the spruce MYB genes are consistent with previous reports describing the putative function of homologous genes For example, ... Phylogenetic relationships and gene family structure of conifer R2R3-MYBs We used the Mega 2.0 method to construct a phylogenetic tree using full length cDNA sequences (Fig 2) The result of our...

Ngày tải lên: 12/08/2014, 05:20

... in gene expression: meaning and relevance to the cell cycle It is of interest to look at the basic data in Table of Liu, Gaido and Wolfinger [1] In Table they present results on the gene expression ... recombined together and analyzed These combined cells should not give a variable pattern of gene expression This controls for perturbation of the culture by the selection method 15) Results using different ... different experiments in terms of variation in the phase angles of peaking of gene expression in different individual cells Thus, given a particular variation in gene expression in different cells,...

Ngày tải lên: 13/08/2014, 23:20

... of production relative to its rate of degradation The quantity of a protein produced in the cell is affected by the expression level of its mRNA transcript The levels of gene expression are controlled ... between transcript expression levels and the amount of disorder are not dictated by function class bias and represent genuine and robust features of the data Absolute gene expression profiles for highly ... the down-regulation of gene expression The absolute gene expression levels and the predicted miRNA regulation are anti-correlated The overall decrease in the gene expression of the transcripts...

Ngày tải lên: 14/08/2014, 21:20

... Effect of hypoxia and over -expression of hChK on invasion potential of PC-3 and LNCaP prostate cancer cells 87 Figure 31 Effect of hypoxia and over -expression of hChK on expression of promigratory ... Effect of over -expression of hChK on cell morphology of prostate cancer cells 84 Figure 29 Effect of over -expression of hChK on anchorage independent growth of LNCaP prostate cancer ... over -expression of choline kinase and hypoxia on invasion potential of prostate cancer cells 86 4.6 Effect of over -expression of choline kinase and hypoxia on expression of pro-invasion...

Ngày tải lên: 24/08/2014, 10:38