genotyping and gene expression
Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt
... in the nucleus and binds to and regulatesbasal expression via HSEs of target genes [26].Deletion of the HSF gene is lethal to cells, even atphysiological temperatures. The genes targeted byHSF ... involvednot only in heat shock protein (HSP) gene expression and stress resistance, but also in the expression ofgenes with roles in cell maintenance and differentia-tion, as well as in developmental ... basal HSP expression [38]. Although HSF1 gains DNA-binding and transactivating abilities upon stress, a little HSF1trimer is present constitutively in cells and binds to and regulates the expression...
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Tài liệu Báo cáo khoa học: Time-dependent regulation analysis dissects shifts between metabolic and gene-expression regulation during nitrogen starvation in baker’s yeast doc
... gene expression of a certain gene (X) normalized to the mean ofthe control gene PDI1 and the steady-state sample of thechemostat culture (Eqns 6 and 7). We have normalized tosteady state and ... substrates, products and effectors (X), and by changes in the affinities(1 ⁄ K) of enzyme e towards its substrates, products and effectors (K). As derived previously [6,7], gene expression and metabolic ... versus gene expression before examiningspecific metabolites.Earlier regulation analysis studies of nitrogen starva-tion in yeast revealed mixed and diverse regulation [9].Both gene expression and...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
... 5).BAT-specific gene expression We next determined gene expression in different adi-pose depots in response to PM2.5exposure, in terms ofthe expression of BAT-specific and WAT-specific gene profiles ... inducesoxidative stress and alterations of mitochondria and gene expression inbrown and white adipose tissues. Particle and Fibre Toxicology 2011 8:20.Submit your next manuscript to BioMed Central and take ... homeoboxfamily of genes, and it i s recognized as WAT-specificmarker in primary adipocyte cultures [29]. DPT servesas a good gene marker for white adipogenesis and canbe seen as a reference gene for...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
... ARTICLENuclear actin and actin-binding proteins in the regulationof transcription and gene expression Bin Zheng1, Mei Han1, Michel Bernier2 and Jin-kun Wen11 Department of Biochemistry and Molecular ... activation and actin polymerization [55].Recently, MRTF-A and -B were shown to serve as alink between STARS and SRF. In NIH 3T3 cellscotransfected with expression plasmids encodingMRTFs and STARS, ... recentdevelopments in the study of transcription and transcriptional regulation bynuclear actin, and the regulation of muscle-specific gene expression, nuclearreceptor and transcription complexes by ABPs....
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
... set, ND4, and SMG-1 genes in human spontaneous lymphomas and normal B- and T-lymphocytes. (a) 32P-labeled PCR-fragments of the set and a-myb oncogenes, and ND4, pub and capn4 genes were ... h1) and SMG-1, the gene of ribosomal protein S8 (in case of lymphoma h2), as well as several genes of unknown function (9 and 8 in the case of lymphoma h1 and h2, respectively). The latter genes ... HIV-associated and monkey SIV-associated lymphomas and seemed to be common for virus-specific lymphomagenesis in human and monkey. Table 4. Selected human HIV-associated lymphomas h1 and h2 genes...
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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx
... critical for HOX gene regulation and embryonic developmentHomeobox genes are a group of evolutionarily con-served genes that encode transcription factors and reg-ulate gene expression during ... FEBSMINIREVIEWMixed lineage leukemia: roles in gene expression, hormonesignaling and mRNA processingKhairul I. Ansari and Subhrangsu S. MandalDepartment of Chemistry and Biochemistry, The University of ... MLL3, MLL4, MLL5, Set1A and Set1B, and each possesses histone H3 lysine 4 (H3K4)-specific methyltrans-ferase activity and has critical roles in gene activation and epigenetics.Although MLLs...
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Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc
... thenucleus and the cytoplasm. Genes Dev 12, 55–66.31 Gui JF, Tronche`re H, Chandler SD & Fu XD (1994)Purification and characterization of a kinase specific forthe serine- and arginine-rich ... interact-ing candidates and were treated witharsenite (1 mM for 30 min). Cells were fixed and stained with mouse anti-HA and goatanti-TIAR sera. Secondary Alexa594-coupled donkey anti-mouse (red) and FITC-coupled ... associated with TIAR and TIA-1 proteincomplexes and is a bona fide SG componentBecause ASF ⁄ SF2 and TIAR shared similar localiza-tion patterns both in normal and stressed cells, weAMinigenekDa+–+–kDaRHII/Gu/DDX21*******UBF1hnRNP...
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Tài liệu Báo cáo khoa học: Hsp105b upregulates hsp70 gene expression through signal transducer and activator of transcription-3 pdf
... hsp70 and hsp90 promoters [21]. As we showed, in this study, thatSTAT3 directly bound to the region between )206 and )187 bp of the hsp70 promoter and enhanced the expression of the hsp70 gene ... for 5 h, and the expression of STAT3, Hsp70 and a-tubulin was analyzed by wes-tern blotting, using the respective antibodies. (B) The density ofbands was quantified by densitometry, and was corrected ... LatchmanDS (1999) Signal transducer and activator of transcrip-tion-1 and heat shock factor-1 interact and activate thetranscription of the Hsp-70 and Hsp-90beta gene promoters. J Biol Chem 274,...
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Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
... inducerof uPA gene expression [16].Decma-induced uPA gene expression isdependent on Erk activationWe and others have shown that activation of Erk playsan important role in uPA gene expression ... E-cadherin distribution, cell scattering and uPA expression. (A) T47D cells (a and b) and MCF7 cells(c and d) were treated for 4 h with control or Decma supernatant and immunostained with anti-E-cadherin ... Basel, Switzerland) and details will be pub-lished elsewhere.Plasmids and siRNAsConstruction of expression vectors for HA-tagged full-length mouse p46shc, p52shc, and p66shc and the introduct-ion...
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Tài liệu Báo cáo khoa học: Modulation of oat arginine decarboxylase gene expression and genome organization in transgenic Trypanosoma cruzi epimastigotes docx
... Foreign ADC gene expression in T. cruziFEBS Journal 273 (2006) 628–637 ª 2006 The Authors Journal compilation ª 2006 FEBS 633Modulation of oat arginine decarboxylase gene expression and genome ... during the transient expression of the foreign gene. After-wards, the free plasmid disappeared almost completely for several weeks and, finally, when the expression of the ADC gene became stable, ... natural deletion mutant for ODC and ADC genes, we used these polyamine auxotrophic par-asites as recipients of foreign ODC or ADC genes tostudy their expression and the eventual suppression ofpolyamine...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... action of EPA and DHA (Eur. J. Biochem. 271) 4473Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cellsby n-6 and n-3 polyunsaturated ... promoter activity is dependentupon the R as/Raf-1/MEK/ERK and PI3-K pathways, and EPA and DHA act in opposition to AA by preventing egr-1 gene expression in IL-1b-treated cells. However, transienttransfection ... may contribute to the modu-lation of egr-1 gene expression (Fig. 4B).Effects of n-3 and n-6 PUFAs on the synthesisof caveolin-1 and caveolin-3 and on the locationof p42/44 MAPK caveolaeCaveolins...
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Tài liệu Báo cáo Y học: Inhibition of the MEK/ERK signaling pathway by the novel antimetastatic agent NAMI-A down regulates c-myc gene expression and endothelial cell proliferation ppt
... downregulated c-myc gene expression to the same extent in serum-cultured cells and dose-dependently counteracted, and ultimately abolished, the increase in c-myc gene expression elicited by ... regulatesc-myc gene expression To investigate whether NAMI-A-induced inhibition ofERK1/2 signaling could affect the expression of a cellgrowth-related gene, we assessed c-myc gene expression ingrowing ... control (individual densities from ERK1 and 2 bands were added up to generate one single ERK1/2 value).Ó FEBS 2002 NAMI-A affects c-myc gene expression and MEK/ERK phosphorylation (Eur. J. Biochem....
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Plant physiology - Chapter 14 Gene Expression and Signal Transduction potx
... regions called exons and noncoding regions Gene Expression and Signal Transduction5 Gene EDNAmRNA Gene D Gene C Gene B Gene A Gene E Gene D Gene C Gene B Gene ATryptophan operonTranslationTranscriptionmRNATranslationTranscriptionRepressor ... biosynthetic pathways in which one or Gene zDNA Gene y Gene aOperator oLactose operonCAP–cAMPcomplexRNA polymerase5′3′(A) CAPCyclic AMP(cAMP) Gene zDNA Gene y Gene aOperator oTranscriptionoccursmRNA5′3′(B)Regulatory gene ... describedabove and summarized in Figure 14.5, the expression ofmost eukaryotic genes, like their prokaryotic counterparts,appears to be regulated at the level of transcription. Gene Expression and Signal...
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Báo cáo khoa học: Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways Role of CREB and AP-1 transcription factors doc
... Cyr61/CCN1 gene expression and RhoA and/ or p38 activation. In particular, mechanical stretch and contractile agonists such as thrombin are well-knownregulators of both Cyr61/CCN1 gene expression and ... mRNA and protein levels. Wecompared the expression profile of the Cyr61/CCN1 gene tothat of the CTGF/CCN2 gene and showed that S1Pcoordinately regulates the expression of both Cyr61/CCN1 and ... identify cytoplasmic and nuclear events thatcould activate Cyr61/CCN1 gene expression. We havedemonstrated that S1P stimulates Cyr61/CCN1 gene expression through RhoA GTPase and that additionalsignaling,...
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Báo cáo khoa học: Efficient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc
... this expression system is of interest forendogenous generation of siRNA and activation of theRNAi effect in human cells [45]. Studies on an inhibitionof BACE gene expression by endogenously generatedsiRNA ... [38]. Selective inactivationof genes for BACE1 and BACE2 by siRNA was reportedrecently [39]. Up to now several expression systems havebeen designed for endogenous generation of siRNA [40–44].As ... electrophoresis and stainedwith ethidium bromide. For experiments performed in co-amplification conditions two pairs of primers P1BACE and P2BACE,forBACEmRNA(20lM), and P1GAPDH and P2GAPDH,...
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