genotyping and gene expression

... in the nucleus and binds to and regulates basal expression via HSEs of target genes [26]. Deletion of the HSF gene is lethal to cells, even at physiological temperatures. The genes targeted by HSF ... involved not only in heat shock protein (HSP) gene expression and stress resistance, but also in the expression of genes with roles in cell maintenance and differentia- tion, as well as in developmental ... basal HSP expression [38]. Although HSF1 gains DNA-binding and transactivating abilities upon stress, a little HSF1 trimer is present constitutively in cells and binds to and regulates the expression...

Ngày tải lên: 18/02/2014, 04:20

... gene expression of a certain gene (X) normalized to the mean of the control gene PDI1 and the steady-state sample of the chemostat culture (Eqns 6 and 7). We have normalized to steady state and ... substrates, products and effectors (X), and by changes in the affinities (1 ⁄ K) of enzyme e towards its substrates, products and effectors (K). As derived previously [6,7], gene expression and metabolic ... versus gene expression before examining specific metabolites. Earlier regulation analysis studies of nitrogen starva- tion in yeast revealed mixed and diverse regulation [9]. Both gene expression and...

Ngày tải lên: 18/02/2014, 06:20

... 5). BAT-specific gene expression We next determined gene expression in different adi- pose depots in response to PM 2.5 exposure, in terms of the expression of BAT-specific and WAT-specific gene profiles ... induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues. Particle and Fibre Toxicology 2011 8:20. Submit your next manuscript to BioMed Central and take ... homeobox family of genes, and it i s recognized as WAT-specific marker in primary adipocyte cultures [29]. DPT serves as a good gene marker for white adipogenesis and can be seen as a reference gene for...

Ngày tải lên: 06/03/2014, 19:20

... ARTICLE Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression Bin Zheng 1 , Mei Han 1 , Michel Bernier 2 and Jin-kun Wen 1 1 Department of Biochemistry and Molecular ... activation and actin polymerization [55]. Recently, MRTF-A and -B were shown to serve as a link between STARS and SRF. In NIH 3T3 cells cotransfected with expression plasmids encoding MRTFs and STARS, ... recent developments in the study of transcription and transcriptional regulation by nuclear actin, and the regulation of muscle-specific gene expression, nuclear receptor and transcription complexes by ABPs....

Ngày tải lên: 07/03/2014, 01:20

Ngày tải lên: 18/06/2014, 15:20

Ngày tải lên: 18/06/2014, 16:20

... set, ND4, and SMG-1 genes in human spontaneous lymphomas and normal B- and T- lymphocytes. (a) 32 P-labeled PCR-fragments of the set and a-myb oncogenes, and ND4, pub and capn4 genes were ... h1) and SMG-1, the gene of ribosomal protein S8 (in case of lymphoma h2), as well as several genes of unknown function (9 and 8 in the case of lymphoma h1 and h2, respectively). The latter genes ... HIV-associated and monkey SIV-associated lymphomas and seemed to be common for virus-specific lymphomagenesis in human and monkey. Table 4. Selected human HIV-associated lymphomas h1 and h2 genes...

Ngày tải lên: 02/11/2012, 11:08

... critical for HOX gene regulation and embryonic development Homeobox genes are a group of evolutionarily con- served genes that encode transcription factors and reg- ulate gene expression during ... FEBS MINIREVIEW Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing Khairul I. Ansari and Subhrangsu S. Mandal Department of Chemistry and Biochemistry, The University of ... MLL3, MLL4, MLL5, Set1A and Set1B, and each possesses histone H3 lysine 4 (H3K4)-specific methyltrans- ferase activity and has critical roles in gene activation and epigenetics. Although MLLs...

Ngày tải lên: 16/02/2014, 14:20

... the nucleus and the cytoplasm. Genes Dev 12, 55–66. 31 Gui JF, Tronche ` re H, Chandler SD & Fu XD (1994) Purification and characterization of a kinase specific for the serine- and arginine-rich ... interact- ing candidates and were treated with arsenite (1 m M for 30 min). Cells were fixed and stained with mouse anti-HA and goat anti-TIAR sera. Secondary Alexa 594-coupled donkey anti-mouse (red) and FITC-coupled ... associated with TIAR and TIA-1 protein complexes and is a bona fide SG component Because ASF ⁄ SF2 and TIAR shared similar localiza- tion patterns both in normal and stressed cells, we A Minigene kDa +–+– kDa RHII/Gu/DDX21 * * * * * * * UBF1 hnRNP...

Ngày tải lên: 16/02/2014, 15:20

... hsp70 and hsp90 promoters [21]. As we showed, in this study, that STAT3 directly bound to the region between )206 and )187 bp of the hsp70 promoter and enhanced the expression of the hsp70 gene ... for 5 h, and the expression of STAT3, Hsp70 and a-tubulin was analyzed by wes- tern blotting, using the respective antibodies. (B) The density of bands was quantified by densitometry, and was corrected ... Latchman DS (1999) Signal transducer and activator of transcrip- tion-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90beta gene promoters. J Biol Chem 274,...

Ngày tải lên: 18/02/2014, 06:20

... inducer of uPA gene expression [16]. Decma-induced uPA gene expression is dependent on Erk activation We and others have shown that activation of Erk plays an important role in uPA gene expression ... E-cadherin distribution, cell scattering and uPA expression. (A) T47D cells (a and b) and MCF7 cells (c and d) were treated for 4 h with control or Decma supernatant and immunostained with anti-E-cadherin ... Basel, Switzerland) and details will be pub- lished elsewhere. Plasmids and siRNAs Construction of expression vectors for HA-tagged full- length mouse p46 shc , p52 shc , and p66 shc and the introduct- ion...

Ngày tải lên: 19/02/2014, 05:20

... Foreign ADC gene expression in T. cruzi FEBS Journal 273 (2006) 628–637 ª 2006 The Authors Journal compilation ª 2006 FEBS 633 Modulation of oat arginine decarboxylase gene expression and genome ... during the transient expression of the foreign gene. After- wards, the free plasmid disappeared almost completely for several weeks and, finally, when the expression of the ADC gene became stable, ... natural deletion mutant for ODC and ADC genes, we used these polyamine auxotrophic par- asites as recipients of foreign ODC or ADC genes to study their expression and the eventual suppression of polyamine...

Ngày tải lên: 19/02/2014, 07:20

... action of EPA and DHA (Eur. J. Biochem. 271) 4473 Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated ... promoter activity is dependent upon the R as/Raf-1/MEK/ERK and PI3-K pathways, and EPA and DHA act in opposition to AA by preventing egr-1 gene expression in IL-1b-treated cells. However, transient transfection ... may contribute to the modu- lation of egr-1 gene expression (Fig. 4B). Effects of n-3 and n-6 PUFAs on the synthesis of caveolin-1 and caveolin-3 and on the location of p42/44 MAPK caveolae Caveolins...

Ngày tải lên: 19/02/2014, 16:20

... down regulated c-myc gene expression to the same extent in serum- cultured cells and dose-dependently counteracted, and ultimately abolished, the increase in c-myc gene expression elicited by ... regulates c- myc gene expression To investigate whether NAMI-A-induced inhibition of ERK1/2 signaling could affect the expression of a cell growth-related gene, we assessed c-myc gene expression in growing ... control (individual densities from ERK1 and 2 bands were added up to generate one single ERK1/2 value). Ó FEBS 2002 NAMI-A affects c-myc gene expression and MEK/ERK phosphorylation (Eur. J. Biochem....

Ngày tải lên: 21/02/2014, 01:21

... regions called exons and noncoding regions Gene Expression and Signal Transduction 5 Gene EDNA mRNA Gene D Gene C Gene B Gene A Gene E Gene D Gene C Gene B Gene A Tryptophan operon Translation Transcription mRNA Translation Transcription Repressor ... biosynthetic pathways in which one or Gene zDNA Gene y Gene a Operator o Lactose operon CAP–cAMP complex RNA polymerase 5′ 3′ (A) CAP Cyclic AMP (cAMP) Gene zDNA Gene y Gene a Operator o Transcription occurs mRNA 5′ 3′ (B) Regulatory gene ... described above and summarized in Figure 14.5, the expression of most eukaryotic genes, like their prokaryotic counterparts, appears to be regulated at the level of transcription. Gene Expression and Signal...

Ngày tải lên: 07/03/2014, 21:20

... Cyr61/CCN1 gene expression and RhoA and/ or p38 activation. In particular, mechanical stretch and contractile agonists such as thrombin are well-known regulators of both Cyr61/CCN1 gene expression and ... mRNA and protein levels. We compared the expression profile of the Cyr61/CCN1 gene to that of the CTGF/CCN2 gene and showed that S1P coordinately regulates the expression of both Cyr61/ CCN1 and ... identify cytoplasmic and nuclear events that could activate Cyr61/CCN1 gene expression. We have demonstrated that S1P stimulates Cyr61/CCN1 gene expression through RhoA GTPase and that additional signaling,...

Ngày tải lên: 08/03/2014, 08:20

... this expression system is of interest for endogenous generation of siRNA and activation of the RNAi effect in human cells [45]. Studies on an inhibition of BACE gene expression by endogenously generated siRNA ... [38]. Selective inactivation of genes for BACE1 and BACE2 by siRNA was reported recently [39]. Up to now several expression systems have been designed for endogenous generation of siRNA [40–44]. As ... electrophoresis and stained with ethidium bromide. For experiments performed in co- amplification conditions two pairs of primers P1 BACE and P2 BACE ,forBACEmRNA(20l M ), and P1 GAPDH and P2 GAPDH ,...

Ngày tải lên: 08/03/2014, 08:20

Ngày tải lên: 16/03/2014, 02:20

Ngày tải lên: 16/03/2014, 16:20

Ngày tải lên: 16/03/2014, 18:20