Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death

... I.5 Na+- H+ Exchanger (NHE) The Na+- H+ exchangers (NHEs) are a family of membrane glycoproteins which transport H+ out of the cell in exchange for Na+ with a stoichiometry of 1: 1 In mammalian cells, ... increased 11 2 susceptibility to cell death IV .12 Regulation of intracellular pH as one of the mechanisms of 11 3 NHE- 1 -mediated cell...

Ngày tải lên: 16/09/2015, 08:31

... is usually the result of their hydrophobicity [28,33] The Na+/H+ exchanger is predicted to have 12 transmembrane segments and one membrane- associated Fig Colocalization of the Na+/H+ exchanger ... of membrane fractions from methyl b-cyclodextrin-treated cells of the Na+/H+ exchanger, biochemical characterization of the properties of the full length...

Ngày tải lên: 08/03/2014, 16:20

... Tet -On HIV-1 system were used [41,42] Activation of HIV-1 gene expression was achieved by either addition of doxycyline (Dox) at a concentration of μg/ ml or transfection with the constitutively ... Expression of a catalytically inactive form of CLK2 Wong et al Retrovirology 2011, 8:47 http://www.retrovirology.com/content/8/1/47 Page of 12 Figure Effect of CLK Overe...

Ngày tải lên: 13/08/2014, 01:20

... Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells Journal of Experimental & Clinical Cancer Research ... suggest that apoptosis is involved in the potentiation of cytotoxicity caused by saikosaponins and cisplatin co-treatment Saikosaponins induce in...

Ngày tải lên: 10/08/2014, 10:20

... results indicate that oridonin induced autophagy < /b> in L929 cells Inhibition of autophagy < /b> up-regulates apoptosis < /b> in oridonin-induced L929 cells To investigate the role of autophagy < /b> in oridonininduced apoptosis < /b> ... with Beclin or LC3 siRNA also increased oridonin-induced cell apoptosis < /b> (Fig 9D) These findings demonstrate that the inhibition o...

Ngày tải lên: 18/02/2014, 13:20

... Nck [72] The binding of these proteins could prevent the interaction of the Ca2+ ⁄ CaM complex to the receptor if the CaMBD were occluded at least in part Nevertheless, the docking of these adaptor ... internalization and the subsequent degradation of the receptor in transfected human embryonic kidney cells [50] Direct regulation of the EGFR by CaM The dire...

Ngày tải lên: 06/03/2014, 09:22

... Regulation of activity and expression of NHE 1 54  1. 4.4 A  1. 4.4 B  1. 5  Regulation of NHE -1 activity 54  Transcriptional regulation of NHE -1 expression .58  AIM OF STUDY 61 ... response repression of NHE -1 expression .11 1  Figure 13 : Thiol oxidizing agent diamide mimicked the effect of H2O2 in the downregulation of NHE -1 gene expression 11 3  Figu...

Ngày tải lên: 14/09/2015, 08:50

... MOLECULAR FUNCTION AND REGULATION OF THE BAX- ASSOCIATING PROTEIN MOAP- 1 Fu Nai Yang INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2007 MOLECULAR FUNCTION AND REGULATION ... pore 35 1. 3.3.3 Channel formation 38 ii 1. 4 THE MULTIDOMAIN PRO-APOPTOTIC PROTEIN BAX AND BAK 41 1.4 .1 Bax plays dominant role over Bak 41...

Ngày tải lên: 14/09/2015, 12:54

... human and a murine NICN1 cDNA clone from the IMAGE collection and determined the complete sequences of these cDNAs The comparative analysis of the human, canine, and murine NICN1 cDNA revealed a ... CACCAGgtcagctgggcctca 423 418 bp (exon 3, 11 4 bp) … CCAAAGgcaagtgactttgca 4 01 bp (exon 4, 72 bp) 495 … CTTGAGgtaagctctctaaca 3 71 bp (exon 5, 10 5 bp) 600 … TTCGACgtgag...

Ngày tải lên: 23/03/2014, 21:20

... 5¢-CACACTACACTGGGAAGCAGAGACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG3¢ The cDNA was subcloned into the EcoRV site of pBluescript SK(+) (Stratagene, La Jolla, CA, USA) and sequenced using an automated ... Na -palmitoylation of Gas is regulated also remains unclear However, we assume that mammalian Gup1 competes with Skn for Shh to prevent palmitoylation rather than catalyzing...

Ngày tải lên: 18/02/2014, 16:20

... in contact with the end surfaces of the wires were obtained by annealing at 250 1C for 20 Using the Pd2Si contacts as source and drain and the silicon substrate as a back-gate, a transistor configuration ... configuration, contact geometry and charge distribution are demonstrated in Fig The preparation started from an SOI wafer with a silicon film thickness of 55 nm and a burie...

Ngày tải lên: 16/03/2014, 15:14

... cholesterol in macrophages that transform into foam cells This effect is restored by the addition of prostaglandins AA, arachidonic acid; ABCA1, ATP-binding cassette transporter A1 ; COX, cyclooxygenase; ... Statistical analysis of experimental data Statistical analysis was performed using SigmaStat version 2.03 (SPSS Inc., Chicago, IL, USA) Data was analyzed using the Kruskal-W...

Ngày tải lên: 09/08/2014, 10:20

... indicate that LIS1 might directly inhibit PP2A and that the inhibition of PP2A is likely to be mediated by WD domain(s) of LIS1 Binding of Tat to LIS1 does not affect the inhibition of PP2A by LIS1 We ... activity, % 100 80 PP2A 60 PP1 40 20 Figure LIS1 inhibition of PP2A is not altered by Tat LIS1 inhibition of PP2A is not altered by Tat Ph...

Ngày tải lên: 13/08/2014, 09:20

... uncoupling of the Na+-NQR activity by dioxygen Characterization of redox cofactors in membranes from V alginolyticus In the presence of NADH and dioxygen, the purified Na+NQR from V alginolyticus ... the degree of uncoupling of the enzyme The term ÔuncouplingÕ describes the observation that the electrons derived from the oxidation of NADH by the Na...

Ngày tải lên: 31/03/2014, 21:21

Ngày tải lên: 14/11/2014, 06:34