Genetic study of hematopoiesis in zebrafish characterization of zebrafish udu mutant, positional cloning and functional study of udu gene

... GENETIC STUDY OF HEMATOPOIESIS IN ZEBRAFISH — CHARACTERIZATION OF ZEBRAFISH UDU MUTANT, POSITIONAL CLONING AND FUNCTIONAL STUDY OF UDU GENE LIU YANMEI (Master of Medicine, Peking University, ... differentiation of hematopoietic cells in udu- /- 94 4.1.2 Identification of the udu mutant gene 100 4.1.2.1 Positional cloning of udu gene...

Ngày tải lên: 14/09/2015, 17:54

... organism for cloning the genes encoding the different fatty acid desaturases involved in EPA biosynthesis and identified two sequences coding for fatty acid desaturases with D5- and D6-regioselectivities ... contains 12 acidic amino acids (aspartate and glutamate), whereas the corresponding domain sequences in PtD5p and PtD6p have only eight and five acidic residues,...

Ngày tải lên: 31/03/2014, 09:20

... highly pathogenic avian influenza were retrieved from the database and included in the analysis human, classical swine and Eurasian swine lineages (Fig 1) The avian NP lineage contains all avian influenza ... (PB1), avian lineage (PB2, PA), Eurasian swine lineage (NA, M) and classical swine lineage (HA, NP, NS) [14,15] This serves as an example that certain influenza A str...

Ngày tải lên: 12/08/2014, 04:20

... appearance of other d-amino acids This is the first report, for eukaryotes, of cDNA cloning and functional characterization of d-AAT acid aminotransferase in A thaliana tion rate of main roots and hypocotyls ... between d-amino acids, was cloned and characterized This represents the first cDNA cloning and functional characterization of a d-AAT of eukary...

Ngày tải lên: 30/03/2014, 04:20

... characterization of the human VGSC b1B subunit The human b1B subunit is a novel splicing variant of the b1 subunit via alternative intron retention The retained intron encodes a novel extracellular, ... peptide Functional expression of the human b1B subunit with NaV1.2 in Xenopus oocytes To explore the regulatory function of the hum...

Ngày tải lên: 16/03/2014, 23:20

... Results Cloning of the 5¢-upstream region of the Pokemon gene To identify the regulatory sequences that control expression of the Pokemon gene, a 2204-bp section of the 5¢-upstream region of the Pokemon ... mechanisms of the Pokemon gene Computer analysis of putative transcription factor-binding sites For a rough understanding of the re...

Ngày tải lên: 30/03/2014, 04:20

... E.P., Martin-Eauclaire, M.F., Mansuelle, P & Sampieri, F (1991) An anti-insect toxin purified from the scorpion Androctonus australis Hector also acts on the alpha- and beta-sites of the mammalian ... Sampieri, F & Granier, C (1991) An anti-insect toxin purified from the scorpion Androctonus australis Hector also acts on the a- and b-sites of the mammalian s...

Ngày tải lên: 31/03/2014, 09:20

... GENETIC STUDY OF HEMATOPOIESIS DEVELOPMENT BY TWO ZEBRAFISH MUTANTS: UGLY DUCKLING AND TC- 244 DU LINSEN (B.Sc, Zhejiang University, China) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... analysis of zebrafish mutants udu and tc- 244 in this study, two novel genes (udu and tc- 244) are identified as novel factors involved in the re...

Ngày tải lên: 13/09/2015, 20:35

... analyses Integration of this project with the larger NHSII study This study will take advantage of the resources of the ongoing NHSII study, whose core functions including the infrastructure of ... after the interview If the error is detected again in following interviewers, the interviewer is removed from the study Use of highly structured CATI interviews...

Ngày tải lên: 11/08/2014, 17:20

... Zohari et al., Full genome comparison and characterization of avian H10 viruses with different pathogenicity in Mink (Mustela vison) reveals genetic and functional differences in the non-structural ... of the influenza virus [19] The amino acid composition of the receptor binding pocket of the HA protein for the H10 isolates...

Ngày tải lên: 12/08/2014, 04:20

... The glycoprotein serine protease plasminogen activator inhibitor-1 (PAI-1) is a key element in the inhibition of fibrinolysis The primary role of PAI-1 in vivo is fast acting inhibition of tissue- ... shows genetic association between the 4G allele of 4G/5G polymorphism and the occurrence of MODS and septic shock in pneumonia sepsis Comparing the allele fre...

Ngày tải lên: 13/08/2014, 20:22

... clinical evaluation of patients, samples and data collection PS participated in the statistical analysis NGQ, IS and EHR did genotyping CRG conceived the study, analyzed and interpreted data, and wrote ... overrepresented among patients who died at 28 or 90 days, and they also predisposed to MODS and ARDS respectively Likewise, 6A was associated with ARDS, and 1A was...

Ngày tải lên: 14/08/2014, 07:21

... paracrine signaling in liver development The coexpression of hgfa and c- met in pectoral fin, hgfb and c- met in proneprhic duct also indicate their paracrine signaling in the development of these ... Characterizing HGF and its receptor c- met s role in zebrafish development (Manuscript in preparation) v LIST OF FIGURES Fig.1.1 Schematic represent...

Ngày tải lên: 12/09/2015, 08:18

... 1.1.3.1 Pin1 function in M phase 1.1.3.2 Pin1 function in G1 and S phase 1.1.3.3 Pin1 function in oncogenesis .9 1.1.4 Pin1 function in apoptosis 10 1.1.5 Pin1 function in ... reduction of zPin1 expression in zPin1 morphant embryos 79 Figure 3.8 Developmental delay in zPin1 morphant embryos 82 Figure 3.9 Neurogenin1 staining of the wild type embryos and c...

Ngày tải lên: 12/09/2015, 09:55

... mouse, Fugu, and zebrafish 63 -64 3.5 Assembled sequences of irf6 genomic fragments 64 -66 3 .6 The irf6 gene locus on the LG22 in T51 RH panel and in the Ensembl zebrafish version (ZV6) 68 -69 3.7 Whole-mount ... morpholinos 85 3.14 Loss of irf6 function phenotypes 86- 87 3.15 Comparison of expression of molecular markers in irf6 morphants and wildtype 91-92 3...

Ngày tải lên: 14/09/2015, 12:44