... are in agreement with those of a recent work in which GatY has been identified by other means as an invivo substrate of GroEL [27] Interestingly, GatY is also included in a list of proteins that ... Services Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was ... protein family [15] E coli contains five small members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this...
... Phosphoinositide 3-kinase Promyelocytic Leukemia Zinc Finger protein Phosphatase of Regenerating Liver Retinoblastoma Protein Red Fluorescence Protein RNA interference Receptor Tyrosine Kinase ... Shock Protein 90 Indirubin Derivative Interferon gamma-inducible gene 16 Immunohistochemistry Intraperitoneally Internal Tandem Duplication c-Jun N-terminal Kinase Kinase Insert Domain Receptor ... Nanjing, M.sc National University of Singapore) A THESIS SUBMITTED FOR THE DEGREE OF DORCTOR OF PHILOSOPHY DEPARTMENT OF MEDICINE YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE...
... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) Invivo pharmacokinetics and bioavailability Invivo pharmacokinetic study ... i.v at a dose of 200 mg/kg Invivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma ... drug concentration of EPI and PA/EPI after i.v injection in rats at a single equivalent dose of 10 mg/kg Table 5. Pharmacokinetic parameters of EPI and PA/EPI i.v injected in rats at the equivalent...
... spectrum of the porphyrin contained in Zn-PP-KatA, in a pyridine ⁄ water ⁄ NaOH ⁄ Tween-80 mixture, was not similar to that of the reference solution of Zn-PP in the same solvent, indicating that ... protein, in binding porphyrins It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem is incorporated into soluble and membrane-bound proteins inside ... compounds being taken up into the cell and incorporated into vital haem proteins instead of haem [5] We show here that haem analogues are indeed taken up and can be incorporated into haem proteins,...
... sequences of the GAL genes through its N-terminal DNA binding site Gal80p inhibits the transcriptional activity of Gal4p by binding to its 28 amino acid region at the carboxyl terminal [25–27] In vitro ... reflected by the sequence of interactions; (c) parameters quantifying strength of interactions; and (d) spatial localization of protein in a compartment or shuttling of proteins between compartments ... concentration of Gal3p in the wild-type thus reducing the protein expression as in Model III This confirms the evidence obtained through gel filtering and cross-linking that Gal3p is monomeric in solution...
... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling ... difficult to follow the invivo clearance and turnover of an allergen after inhalation In this study we used a novel approach for specific labelling of proteins in order to investigate how an airborne ... deposited in the airways of mice and metabolized The labelling method involves the incorporation of a selenocysteine residue and the gamma-emitter selenium-75 (75Se) within an engineered C-terminal...
... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G invivoIn order to examine the topology of ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... or light harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and...
... protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was significantly increased ... reproduced in lung, which can explain in part the conflicting results found in adults and children given b-carotene or vitamin A [29] Moreover, in lung of rats injected with high-dose of vitamin A over ... cyclin dependent kinase inhibitor that is a critical target of p53 in facilitating G1 arrest, we also studied the expression of p21WAF1/CIP1, and found that it is increased in chronic-vitamin...
... observed, and this was independent of the kind of genetic lesion or inhibitor used, suggesting that this activating effect was indeed caused by the fatty acid/lipid environment of the ATPase It must ... and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, i.e sterol alteration, and indirect effects of EBI compounds ... cerevisiae, grown in the presence of octanoic acid [37] In this case, only Vmax was affected showing an increase that was accompanied by an increase in the presence of oleic acid in its plasma membrane...
... understanding of the production and function of miRNA invivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms involved ... et al [22] obtained quantitative in vitro images of nine transcripts that were predicted to be miRNA9 targets using the ensemble machinelearning algorithm However, invivo imaging of miRNA regulation ... the in vitro and invivo investigations of miRNA production patterns Second, noninvasive monitoring of miRNAs provides useful information of the possible role of miRNAs involving cellular developments...
... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... M Averna et al Invivo degradation of NOS and HSP90 by calpain B A C D Fig Invivo digestion of NOS and HSP90 in NMS rats during HSD treatment Aliquots (20 lg protein) of rat brain soluble material ... higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig 1C) A Calpain activation in rat brain and aorta following HSD treatment To promote invivo calpain activation,...
... lines, are useful tools to check for invivo roles of PTOX [1–3,8] However, specific and potent inhibitors are also of interest as they would allow inactivation of PTOX at a certain time during ... by Fisher and Rich [20] for quinone binding sites Although speculative at this stage, this consensus sequence was found in various quinone-binding proteins including AOX [20,21] It should be mentioned ... quinone binding site would be expected to be close to iron binding amino acids, which is the case here ([6], Fig 4) This potential quinone-binding site appears to be conserved in PTOX sequences (including...
... tyrosine kinase (Flt-1) and VEGFR-2 or fetal liver kinase (Flk-1) also known as kinase-insert domain-containing receptor (KDR) [2] In animal models of chronic ischemia, manoeuvres that increase ... http://www.translational-medicine.com/content/7/1/41 QK performing ex vivo experiments of vascular reactivity in WKY common carotid rings [12], and then we evaluated invivo the role of this small peptide studying the ... levels by intramuscular injection or vascular infusion of adenoviral vectors encoding for VEGF [5,6], or indirectly, for example by physical training or β2 adrenergic receptor overexpression in ischemic...
... reacting with the murine receptor resulted in a faint stain of the cytoplasm and rarely in staining of the membrane of the tumor cells (Figure 3B) In the more advanced 30 weeks tumors the staining ... analysis Page of 13 revealed elevated htid expression in 81% of the luminal A type tumors (Figure 1A), in 20% of the cases of the luminal B type over expressing ErbB-2 and in 18% of tumors diagnosed ... characterized by positive staining ranging from moderate to intense staining In the context of the potential association of htid expression with the biology of human breast cancers of different subtype...
... of two conditional probabilities: that of being detected in a muscle of a subject with NSAP and that of being detected in a muscle of a subject free of pain If we maintain our understanding of ... and obtain an estimate of the surface representation of the MUP (called an SMUP template) related to each MUPT The SMUP is determined by using the firing times of the main spike of each individual ... assumption of a Gaussian distribution The measure of stability (column marked ψ in Table 4) provides insight into the variability of the means of the Hamilton-Wright et al Journal of NeuroEngineering...
... flexion-extension arc of motion They demonstrated increased strain in the ATFL with increasing plantarflexion, inversion and internal rotation Strain in the CFL was found to increase in dorsiflexion and inversion ... in- vivo The objective of the current study was to measure the change in apparent length of the ATFL and CFL, in- vivo, in uninjured healthy ankles during a dorsiflexion-plantarflexion and supination-pronation ... participating subjects had subjective complaints of ankle pain, previous history of ankle injury requiring treatment, systemic disease, or contraindication for MR imaging Prior to MR scanning, both...
... quantify the fixation of oral implants [15] A limited number of studies prove the feasibility of detecting several forms of femoral implant loosening, in vitro and invivo using techniques based ... detection of the insertion endpoint Figure curing FRF graphs of a typical stem-femur system during cement FRF graphs of a typical stem-femur system during cement curing The evolution of the FRF ... system for intra operative manufacturing and stability testing of hip prostheses In Proceedings of TEHNOMUS XIII, the 13th International conference on New technologies and products in machine manufacturing...
... gas to produce coating with thickness ranging from 40-70 microns (nominal 50 microns) The coating had a minimum total crystallinity of 65% The minimum HA fraction in the crystalline phase was 90% ... finite dissolution rate of crystalline hydroxyapatite provides a continuous source of calcium and inorganic phosphate [18] In our present study, as well as in those reported by others, bone often ... dehydrated in absolute alcohol and de-fatted in 50% mixture of ether and acetone before being placed in 100% alcohol again for 12 hours Methods Implants for intramedullary implantation in rabbit...
... technique that includes cartilage in the estimation of joint contact patterns In summary, we have developed a technique for characterizing invivo glenohumeral joint contact patterns during dynamic ... recommendation on definitions of joint coordinate systems of various joints for the reporting of human joint motion—part II: shoulder, elbow, wrist and hand,” Journal of Biomechanics, vol 38, no ... Englmeier, and F Eckstein, “Effect of abducting and adducting muscle acitivity on glenohumeral translation, scapular kinematics and subacromial space width in vivo, ” Journal of Biomechanics, vol...
... added into microwells and incubated at room temperature for 30 min, followed by three PBS washes Blocking for non-specific binding was performed by adding 100 lL of 3% bovine serum albumin (BSA) ... that GNPs are capable of inducing an antibody response in mice, which indicates that factors other than cytotoxicity may be involved and complicates invivo application of GNPs [16] The current ... albumin (BSA) and incubating for 60 at room temperature, followed by three PBS washes Binding was performed by adding 100 lL of diluted antiserum into microwells and incubating for h at room...