in vivo characterization of ls replacements
Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... are in agreement with those of a recent work in which GatY has been identified by other means as an in vivo substrate of GroEL [27] Interestingly, GatY is also included in a list of proteins that ... Services Department of the Weizmann Institute by a single injection of 150 lg of the purified protein, followed by two booster shots of the same amount of protein at 2-week intervals Serum was collected ... protein family [15] E coli contains five small members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this...
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In vitro and in vivo study of ABT 869 in treatment acute myeloid leukemia (AML) alone or in combination with chemotherapy or HDAC inhibitors insight into molecular mechanism and biologic characterization
... resistant cell lines to FLT3 inhibitors Gene profiling reveals up-regulation of FLT3LG and Survivin, but down-regulation of SOCS genes in MV4-11-R cells Targeting survivin by shRNA induce apoptosis ... Phosphoinositide 3-kinase Promyelocytic Leukemia Zinc Finger protein Phosphatase of Regenerating Liver Retinoblastoma Protein Red Fluorescence Protein RNA interference Receptor Tyrosine Kinase ... progressively The increasing expression of cyclin E in MV4-11 cells at 4h, in MOLM-14 cells at 1h and cyclin D in MOLM-14 cells at 8h after drug exposure could be due to the fact that cells intended to...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma levels over ... University of Technology Sydney on 03/01/11 For personal use only In vivo toxicity To evaluate whether i.v administration (at dose of 200 mg/kg) of PANs was associated with any toxicity in vivo, animals...
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Báo cáo khoa học: In vivo production of catalase containing haem analogues pot
... spectrum of the porphyrin contained in Zn-PP-KatA, in a pyridine ⁄ water ⁄ NaOH ⁄ Tween-80 mixture, was not similar to that of the reference solution of Zn-PP in the same solvent, indicating that ... protein, in binding porphyrins It is not known how haem is transported intracellularly in bacteria, and the mechanisms by which haem is incorporated into soluble and membrane-bound proteins inside ... compounds being taken up into the cell and incorporated into vital haem proteins instead of haem [5] We show here that haem analogues are indeed taken up and can be incorporated into haem proteins,...
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Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt
... sequences of the GAL genes through its N-terminal DNA binding site Gal80p inhibits the transcriptional activity of Gal4p by binding to its 28 amino acid region at the carboxyl terminal [25–27] In vitro ... prevalent in vivo Experimental procedures We consider four candidate models, Models I–IV, shown in Figs 1–3, to validate the mechanism of induction of GAL genes by galactose In each of the models, ... [19] The key mechanism that controls the protein expression in the wild-type is the shuttling of Gal80p Increasing the amount of Gal80p in the cytoplasm by increasing the distribution coefficient...
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Báo cáo khoa học: A mouse model for in vivo tracking of the major dust mite allergen Der p 2 after inhalation docx
... addition, an increased number of eosinophils were observed in lung interstitium of sensitized mice, confirming the eosinophilic response following Der p challenge (Fig 4) The tissue levels of radioactivity ... result of the in ammation in the lungs of sensitized animals Up to now there are few data available on the fate of an allergen after inhalation In this study, we tracked inhaled Der p in vivo using ... selenocysteine residue in a C-terminal Sel-tag [18] Der p carrying the Sel-tag had an intact core sequence and maintained allergen-specific IgE-binding epitopes and the use of a Sel-tag enabled labelling...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... shown in Fig 4C Interestingly, both tags are recognized more efficiently in the context of the PSI-G loop region than at the C-terminus Topology of PSI-G in vivo In order to examine the topology of ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding ... or light harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and...
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Báo cáo khoa học: In vivo studies of altered expression patterns of p53 and proliferative control genes in chronic vitamin A deficiency and hypervitaminosis pot
... compared to controls, while in acute hypervitaminosis the binding was significantly higher These changes in the RAR–DNA binding is not due to different levels of the retinoic acid receptor induced by ... cyclin dependent kinase inhibitor that is a critical target of p53 in facilitating G1 arrest, we also studied the expression of p21WAF1/CIP1, and found that it is increased in chronic-vitamin ... protein extracts were obtained as described in experimental procedures A single band of about 53 kDa was detected in liver and lung indicating that the amount of p53 protein was significantly increased...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
... observed, and this was independent of the kind of genetic lesion or inhibitor used, suggesting that this activating effect was indeed caused by the fatty acid/lipid environment of the ATPase It must ... activity in lmol Pi min)1Æmg)1Æprotein j, No additions; d, + 50 lM vanadate; m, + 30 lM erythrosin B sterols, have on membrane properties As in past reports, the joint use of mutants and inhibitors ... and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, i.e sterol alteration, and indirect effects of EBI compounds...
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Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc
... monitoring of the bioluminescence signals in the same mouse was detected using the Gaussia luciferase reporter system Production of in vivo mature miRNA23 was detected in three cell lines using ... cell lines can be collected and implanted (e.g into the thighs of a mouse) The expression level of the primiRNA transcript can be obtained from living animals by imaging the in vivo bioluminescence ... understanding of the production and function of miRNA in vivo as well as for monitoring the therapeutic potential of miRNAs in cancer Bioimaging of microRNA biogenesis The molecular mechanisms involved...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
... stabilization of proteolytically resistant complexes of NOS with HSP90 are crucial in determining the in vivo resistance of NOS and HSP90 to calpain degradation Results Levels of HSP90 and NOS isozymes in ... higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig 1C) A Calpain activation in rat brain and aorta following HSD treatment To promote in vivo calpain activation, ... [27] This finding can also explain the large extent of digestion of eNOS in aorta, in which, in association with a higher degree of calpain activation, a lower level of HSP90 is also present Identification...
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Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
... conserved Cys in the N-terminal domain of PTOX [3] whilst a conserved Cys in AOX is involved in the stimulation of AOX activity by a-keto acids [13,14,22] When examining the effect of pyruvate on PTOX ... by Fisher and Rich [20] for quinone binding sites Although speculative at this stage, this consensus sequence was found in various quinone-binding proteins including AOX [20,21] It should be mentioned ... quinone binding site would be expected to be close to iron binding amino acids, which is the case here ([6], Fig 4) This potential quinone-binding site appears to be conserved in PTOX sequences (including...
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báo cáo hóa học:" In vivo properties of the proangiogenic peptide QK" pdf
... tyrosine kinase (Flt-1) and VEGFR-2 or fetal liver kinase (Flk-1) also known as kinase-insert domain-containing receptor (KDR) [2] In animal models of chronic ischemia, manoeuvres that increase ... levels by intramuscular injection or vascular infusion of adenoviral vectors encoding for VEGF [5,6], or indirectly, for example by physical training or β2 adrenergic receptor overexpression in ... http://www.translational-medicine.com/content/7/1/41 QK performing ex vivo experiments of vascular reactivity in WKY common carotid rings [12], and then we evaluated in vivo the role of this small peptide studying the...
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Báo cáo hóa học: " In vivo evidence of htid suppressive activity on ErbB-2 in breast cancers over expressing the receptor" potx
... reacting with the murine receptor resulted in a faint stain of the cytoplasm and rarely in staining of the membrane of the tumor cells (Figure 3B) In the more advanced 30 weeks tumors the staining ... analysis Page of 13 revealed elevated htid expression in 81% of the luminal A type tumors (Figure 1A), in 20% of the cases of the luminal B type over expressing ErbB-2 and in 18% of tumors diagnosed ... characterized by positive staining ranging from moderate to intense staining In the context of the potential association of htid expression with the biology of human breast cancers of different subtype...
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báo cáo hóa học: "Bayesian aggregation versus majority vote in the characterization of non-specific arm pain based on quantitative needle electromyography" pot
... of two conditional probabilities: that of being detected in a muscle of a subject with NSAP and that of being detected in a muscle of a subject free of pain If we maintain our understanding of ... and obtain an estimate of the surface representation of the MUP (called an SMUP template) related to each MUPT The SMUP is determined by using the firing times of the main spike of each individual ... examination of the upper extremities, including myotome testing, dermatome (light touch, pin prick) testing, and assessment of the deep tendon reflexes at the C5 to C8 levels Cervical spine range...
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báo cáo hóa học:" Function of anterior talofibular and calcaneofibular ligaments during in-vivo motion of the ankle joint complex" docx
... flexion-extension arc of motion They demonstrated increased strain in the ATFL with increasing plantarflexion, inversion and internal rotation Strain in the CFL was found to increase in dorsiflexion and inversion ... in- vivo The objective of the current study was to measure the change in apparent length of the ATFL and CFL, in- vivo, in uninjured healthy ankles during a dorsiflexion-plantarflexion and supination-pronation ... participating subjects had subjective complaints of ankle pain, previous history of ankle injury requiring treatment, systemic disease, or contraindication for MR imaging Prior to MR scanning, both...
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báo cáo hóa học:" In vivo evaluation of a vibration analysis technique for the per-operative monitoring of the fixation of hip prostheses" pdf
... detection of the insertion endpoint Figure curing FRF graphs of a typical stem-femur system during cement FRF graphs of a typical stem-femur system during cement curing The evolution of the FRF ... system for intra operative manufacturing and stability testing of hip prostheses In Proceedings of TEHNOMUS XIII, the 13th International conference on New technologies and products in machine manufacturing ... der: The influence of contact conditions on resonance frequencies of a hip stemfemur system: a finite element analysis In Proceedings of the International seminar Prediction and evaluation of total...
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báo cáo hóa học:" An in vivo evaluation of bone response to three implant surfaces using a rabbit intramedullary rod model" doc
... gas to produce coating with thickness ranging from 40-70 microns (nominal 50 microns) The coating had a minimum total crystallinity of 65% The minimum HA fraction in the crystalline phase was 90% ... finite dissolution rate of crystalline hydroxyapatite provides a continuous source of calcium and inorganic phosphate [18] In our present study, as well as in those reported by others, bone often ... and tissue engineering of bone and cartilage: inductive signals, stem cells, and biomimetic biomaterials Tissue Eng 2000, 6:351-359 20 Michaels CM, Keller JC, Stanford CM, Solursh M: In vitro cell...
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Báo cáo hóa học: " Research Article In Vivo Measurement of Glenohumeral Joint Contact Patterns" pot
... technique that includes cartilage in the estimation of joint contact patterns In summary, we have developed a technique for characterizing in vivo glenohumeral joint contact patterns during dynamic ... represented the amount of internal/external rotation [18] 2.5 Measuring Glenohumeral Joint Contact Patterns Glenohumeral joint contact patterns were determined by combining the joint motion measured ... four seconds The rate of shoulder motion was controlled using a metronome Subjects performed three trials with a minimum of three minutes between trials to minimize fatigue In addition, biplane...
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Báo cáo hóa học: " Assessment of the In Vivo Toxicity of Gold Nanoparticles" pdf
... added into microwells and incubated at room temperature for 30 min, followed by three PBS washes Blocking for non-specific binding was performed by adding 100 lL of 3% bovine serum albumin (BSA) ... albumin (BSA) and incubating for 60 at room temperature, followed by three PBS washes Binding was performed by adding 100 lL of diluted antiserum into microwells and incubating for h at room ... examination CARS microscopy was performed with a time constant of ms, a scanning area of 300 300 lm, a step size of lm, 300 300 pixels, a scanning velocity of lm/ms, and a sampling rate of 80...
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