Elucidation of transcription factors controlling mouse ES cell pluripotency and differentiation

... role of REST and its cofactors in ES cells ………………110 3.1 Introduction 3.2 Result 3.2.1 The role of REST and its cofactors in ES Cells 3.2.1.1 Dynamic expression profile of REST cofactors in ES Cell ... 3.2.1.2 Depletion of REST cofactors in ES cells 3.2.2 Genome wide mapping of REST and its cofactors’ binding sites in ES Cells 3.2.2.1 Generation of Stable REST co...

Ngày tải lên: 14/09/2015, 14:05

... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein interaction domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context...

Ngày tải lên: 16/02/2014, 09:20

... within )260/)119, we Ó FEBS 2002 Integrin a3 gene promoter (Eur J Biochem 269) 4529 Fig Effects of mutations in the Ets- and GATA-binding sites and the E-box of the mouse a3 integrin gene on promoter ... assay (EMSA) using the Ets consensus site at )133 As the involvement of the Ets consensus binding site at )133 in the promoter activity of th...

Ngày tải lên: 21/02/2014, 03:20

... bHLH regulatory TF network in mouse brain The bHLH regulatory TF network in mouse brain The graph depicts the inferred regulatory network of bHLH TFs (yellow ellipses) and their target genes (pink ... have constructed a transcriptional regulatory network of bHLH TFs in mouse brain using microarray data (gene expression profiles) and the module network metho...

Ngày tải lên: 14/08/2014, 08:20

... pathological roles in RA Other transcription factors Other transcription factors implicated in the pathogenesis of RA are the signal transducer and activator of transcription (STAT) family of proteins, ... particular stimuli Such binding proteins are transcriptional factors In this review, we focus on the role of transcriptional factors on the pathology of rheumatoi...

Ngày tải lên: 18/02/2014, 18:20

... Hultdin et al preliminary data analysis of FocB, a transcription factor regulating fimbrial adhesin expression in uropathogenic Escherichia coli Acta Crystallogr F 66, 33 7–3 41 Altschul SF, Gish ... Interestingly, substitution of Arg61 and Arg81 impair DNA-binding in PapB, indicating that these residues are critical for DNA-binding also in FocB [25] The DNA-...

Ngày tải lên: 15/03/2014, 23:20

... Discussion Transcriptional regulation results from the cooperative binding of transcription factors [12] One of the key determinants of the activation of genes that are under the control of hormone ... compacted chromatin, thereby allowing access for other transcription factors [17] It is therefore tempting to speculate that these features underlie the absence of...

Ngày tải lên: 23/03/2014, 10:20

... article as: Haseneyer et al.: DNA polymorphisms and haplotype patterns of transcription factors involved in barley endosperm development are associated with key agronomic traits BMC Plant Biology ... Carbonero P: An endospermspecific DOF protein from barley, highly conserved in wheat, binds to and activates transcription from the prolamin-box of a...

Ngày tải lên: 12/08/2014, 03:21

... biological systems often display nonlinear dynamic behavior This is probably also the case for the activation of target genes as a result of the combinatorial activity of different transcription factors ... potential quantitative mechanisms of target gene activation, use can be made of gene expression data and knowledge of the available transcriptional gene netwo...

Ngày tải lên: 14/08/2014, 08:20

... transactivation capability or DNA-binding affinity of the other AP-2 family members The crystal structure of the AP-2 proteins has not yet been solved Localization and function AP-2 transcription factors ... AP-2 G gallus AP-2 100 P troglodytes AP-2 * 100 90 87 99 R norvegicus AP-2 * β reports 55 H sapiens AP-2 M musculus AP-2 100 97 H sapiens AP-2 P troglodytes AP-2 * γ...

Ngày tải lên: 14/08/2014, 16:20

... number of targets of transcription factors classified based on conservation range Type of targets Entero-present TFs Widely present TFs* All targets 6.7 ± 8.9 13.9 ± 23.5 66.6 ± 85.2 Repressed targets ... following page) Association between the status of TFs and targets Association between the status of TFs and targets (a) Contingency tables of the presence or...

Ngày tải lên: 14/08/2014, 16:21

... findings underscore the importance of Nanog in maintaining the phenotype of mouse ES cells and in reprogramming other cell types into pluripotent cells These studies show that the level of Nanog ... approach designed to identify novel factors required for self-renewal in mouse ES cells They began with microarray data from mouse ES cells as they progress from an undifferentiated...

Ngày tải lên: 14/08/2014, 17:22

... the split- ubiquitin system The main objective of this analysis is to demonstrate the feasibility of the split- ubiquitin system to detect interactions between human proteins inside human cells and ... linker histone hH1 with the other core histone proteins, hH2A, hH2B, hH3 and hH4 have been analyzed inside human HT1080HPRT- cells with the help of...

Ngày tải lên: 29/09/2015, 13:01

... Groff, and my committee members, Dorothy Flannagan, Elaine Sanders and Pamela Smith without whom this would not have been possible AN EXPERIMENTAL ANALYSIS OF THE FACTORS IMPACTING AUDIT COMMITTEE ... experience of the audit committee financial expert Almost half of the financial experts of the large firms sampled have held the positions of Chief Ex...

Ngày tải lên: 01/06/2014, 13:57