Identification and characterization of novel group 5 and group 21 allergens from dust mite and ige binding epitope mapping of blo t 5

... thermal treatment on IgE- binding activities of Blo t 92 and Blo t 21 4.2.2 Effect of thermal treatment on Blo t and Blo t 21 folding 94 4.2.3 Resistance of IgE- binding of Blo t and Blo t 21 to acid, ... 21 75 and Blo t 3.2.10 Quantitative end point cross-inhibition of Blo t 21 and 77 Blo t 3.2.11 Localization of Blo...

Ngày tải lên: 14/09/2015, 12:44

... allergen from Dermatophagoides farinae: a paralogue of Der f 2? 75 4.1 Introduction 75 4 .2 Identification, isolation and characterization of Der f 22 76 4.3 Genomic organization of Der f 22 and Der f ... antibodies raised against Der f 22 and Der f 2, and immunolocalization on D farinae sections 93 Figure 4. 12 Concentration of Der...

Ngày tải lên: 15/09/2015, 17:09

... MIR-D40 (Sanyo, Osaka, Japan) To amplify the DNA fragments containing a complete x-5 gliadin gene, oligonucleotides, 5¢-AAGTGAGCAATAGTAAACACAAATCAAAC-3¢ and 5¢-CGTTACATTATGCTCCATTGACTAACAACGA TG-3¢, ... on autoradiography film Dot blot immunoassay for inhibition test Expression and purification of recombinant protein Sense (5¢-ATTTCATATGCAACAACAATTCCCCCAGC AACAATCA-3¢) and antis...

Ngày tải lên: 20/02/2014, 01:20

... Superimposition of the NMR structure of Blo t 21 with Blo t and Der p 58 Figure 3 .7 The allergenicity of Blo t 21, Der f 21, Der p and Blo t 60 Figure 3.8 The CD spectrum of Blo t 21, Der f 21, Blo t and ... cells 25 Blot21_E74A_up Blot21_E74A_down Blot21_K76A_down Blot21_K76A_down Blot21_D79A_up Blot21_D79A_down Blot21_E84A_up Blot21_...

Ngày tải lên: 09/09/2015, 18:55

... resistant to cleavage with chymotrypsin, trypsin, papain, or pronase Protozoan parasites of the genus Giardia are one of the earliest lineages of eukaryotic cells, and the Giardia protease is the ... expressed at a high level (data not shown) DISCUSSION Computer analysis of b-expansins reveals significant similarity to cathepsins, which are members of the C1 fami...

Ngày tải lên: 08/03/2014, 22:20

... stages of the parasite Toxoplasma gondii Toxoplasma exists as oocysts, the sexual stage of the parasite, in the guts of cats They can then infect humans or animals that come into close contact with ... causing death to the patient receiving the therapy 75 In addition, it is important to choose the appropriate subsets of T cells to transfer given that there are...

Ngày tải lên: 09/09/2015, 11:31

... the protein Sip1 Sip1 is a nuclear splicing factor containing an arginine/serine-rich domain and a RNAbinding motif that may play a role in linking the processes of transcription and pre-mRNA ... participated in the design and revision of the study and performed the statistical analysis RP participated in the design and revision of the study AS partic...

Ngày tải lên: 09/08/2014, 08:22

... staining (g) Intense staining of a hyperplastic RA synovial lining cell layer This staining was typical of most areas of RA synovium where the lining was hyperplastic (h) An area of an RA synovial ... stained positively (arrow) (e) Intense staining of individual cells in the lining layer of a typical OA synovium (f) An area of an OA synovium demonstrates a lining lay...

Ngày tải lên: 09/08/2014, 10:20

... Discussion and Conclusion Chapter Identification and characterization of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) Venom 3.1 Introduction 3.2 Materials and ... Figure 4.1: Synthetic gene sequences of Fasxiator Figure 4.2: Identification of novel anticoagulants from Bungarus fasciatus venom Figure 4.3: Effects o...

Ngày tải lên: 09/09/2015, 11:08

... Bibliography 195 Appendix 224 Publications 238 vi Summary Identification and characterization of novel proteins from a rare Australian elapid snake Drysdalia coronoides Partial transcriptome from ... venomous terrestrial snakes of the Elapidae family have undergone an extensive radiation in Australia [12] Elapid snake genus Drysdalia from southern Austr...

Ngày tải lên: 11/09/2015, 10:02

... overall incidence was found in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique ... IDENTIFICATION AND CHARACTERIZATION OF A NOVEL HEART- REACTIVE AUTOANTIBODY IN SYSTEMIC LUPUS ERYTHEMATOSUS: POSSIBLE SEROLOGICAL MARKER FO...

Ngày tải lên: 14/09/2015, 12:42

... IDENTIFICATION AND CHARACTERIZATION OF IRON HOMEOSTASIS- RELATED GENES AND HCCDOWN -REGULATED MITOCHONDRIAL CARRIER PROTEIN (HDMCP), A NOVEL LIVER- SPECIFIC UNCOUPLING PROTEIN IN HUMAN HEPATOCELLULAR ... 5’-RACE β2m mitochondrial membrane potential 5'-rapid amplification of cDNA ends β2-microglobulin aa AAH AFP ALAS ANOVA ATP amino acid atypica...

Ngày tải lên: 16/09/2015, 15:55

... acidic proteins (Hathaway and Traugh, 1982) Two distinct casein kinases have been found in many different cell types They have been designated casein kinase (CK1) and casein kinase (CK2) according ... Ca2+/calmodulin-dependent protein kinase II cAMP cyclic adenosine monophosphate Cdk cyclin-dependent kinase Cdk5 cyclin-dependent kinase cDNA complementary deoxyribonucleic a...

Ngày tải lên: 16/09/2015, 17:10

... of polymers 3a, 3b and 3c were measured in chloroform solution The absorption and emission spectra of the polymers are shown in Fig .5. 3 and the values as given in Table 5. 2 The UV spectrum of ... = 5. 2Hz, CCH(O)-, H), 4 .5 (d, J = 5. 5 Hz, O-CH2-, H), 2.7 (s, -OH, H) 13 CNMR ( 75. 4 MHz, CDCl3, δ ppm) 154 .2, 137.2, 134.7, 127.7, 126.7, 126.0, 1 25. 7, 1 25. 5, 121.0,...

Ngày tải lên: 16/09/2015, 17:11

... demonstrated as a natural antiviral defense in plants Is RNAi also antiviral in animals? Flock house virus (FHV), which can infect both animals and plants, was demonstrated as both an initiator and a ... biogenesis and possible roles of miRNAs and siRNAs 1.3.8 Viral suppressor and miRNA-controlled developmental pathway interaction After discovering miRNAs in...

Ngày tải lên: 16/09/2015, 17:11