identification detection and molecular characterization of novel monopartite begomoviruses

... Thesis for the Degree of Ph.D Identification and molecular characterization of rice promoters conferring microspore-preferred expression School of Applied Biosciences, Major in Agronomy ... wish to thank my wife, son, and family for their support, understanding and encouragement during all this time Nguyen Tien Dung VI Identification and characterization of microspore-preferred promoters ... LIST OF TABLES II LIST OF FIGURES III LIST OF APPENDIX V ACKNOWLEDGEMENT VI CHAPTER GENERAL INTRODUCTION CHAPTER IDENTIFICATION AND CHARACTERIZATION...

Ngày tải lên: 06/07/2017, 09:24

... Access Identification and molecular characterization of tissue-preferred rice genes and their upstream regularly sequences on a genome-wide level Shu-Ye Jiang, Jeevanandam Vanitha, Yanan Bai and ... driving the transcription of the genes under their control URS activities can be predicted by the expression profiling of their driven genes Early studies of large-scale of expression analyses were ... [39] and identified overrepresented GO terms (Additional file 5) in all four types of expressed genes A total of three categories of GO terms have been assigned to these genes including molecular...

Ngày tải lên: 27/05/2020, 00:35

... J W Holland, J Zou & C J Secombes (2013) Cloning and characterization of rainbow trout interleukin-17A/F2 (IL-17A/F2) and IL-17 receptor A: expression during infection and bioactivity of recombinant ... like to thank the members of the Joint Graduate Education Program and the 322 Project – Ministry of Education and Training of Vietnam, especially Prof Dr Le Tran Binh, Prof Dr Maria Theresia Schafmeister, ... 76 4.4.3 Characterization of CD56 variability by VASE element 78 4.4.4 Characterization of CD56 variability by MSD element 80 4.4.5 In vivo expression of CD56 isoform transcripts...

Ngày tải lên: 27/03/2020, 07:23

... accurate and prompt diagnosis of diseases while maintaining safety of the personnel (Sola et al., 2014) and detection of Brucella organisms in various clinical, blood and serum samples of livestock and ... 7.74% and 6.16%, still birth 16.00% and 20.00%, retention of placenta 14.22% and 11.71%, repeat breeding 19.70% and 14.35%, orchitis 34.34% and 18.18%respectively by RBPT and i-ELISA Out of 744 ... and Leloglu, N 1996 Detection of Brucella abortus antibodies in cow milk of the Kars area by ELISA and MRT Turkish J Vet Animal Sci., 20(4): 251255 Gumber, S., Aradhana, A., Dhand, N.K and Sandhu,...

Ngày tải lên: 02/07/2020, 23:29

... concentrations of Ampicillin starting from 0.4g/l to g/l Results and Discussion Molecular characterization Enterococcus faecium strain of the Amplification of the 16S rRNA gene of the test strain and sequencing ... growth and assessment of pathogenicity The weight of each of the animal in the experimental and control group were measured on a Sartorius balance before the commencement of the treatment and after ... Assessment and Molecular Characterization of Enterococcus feacium MBTU-P1F1 (KF745071) from Infant feces Anjali Anne Jacob*, T.R Keerthi and S.S Resmi Laboratory of Microbial Biotechnology, School of...

Ngày tải lên: 23/09/2020, 00:35

... Biochemical and Molecular Characterization of Endophytic Bacteria from Tomato (Lycopersicon esculentum Mill.) B Sai Sushma*, B Vidya Sagar, S Triveni and G Uma Devi Department of Plant Pathology, Professor ... Materials and Methods Isolation of endophytic bacteria Sample collection endophytic bacteria and isolation of Healthy tomato plant samples were collected and separated into root, shoot and leaf ... rinse and plated on TSA to check the efficacy of surface sterilization A total of2 4 isolates obtained in this manner were maintained on TSA slants and stored at 4°C Biochemical characterization of...

Ngày tải lên: 28/04/2021, 01:50

... cycles of 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s, and one cycle of 95 °C for 30 s, 60 °C of 30 s, and 95 °C for 30 s for melting-curve analysis Each sample was run in triplicate and ... sera (Lanes and 4), chicken anti-rCSUI_005805 polyclonal sera (Lanes and 6) and negative chicken sera (Lanes and 5), respectively d Immunoblot of naïve merozoite lysate (Lanes and 2) and rCSUI_005805 ... protein band of ~ 25 kDa and only a faint band of ~ 42 kDa (Fig 4C, Lanes 1–5) LC-MS/MS analysis of the 25 kDa band corroborated the presence of a part of the CSUI_005805-coded protein as deduced...

Ngày tải lên: 19/11/2022, 11:47

... Aleksandra4, SIMONOVIĆ Dušan3, BABIĆ Milijana4 Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, Republic of Serbia; 2Institute of Meat Hygiene and Technology, ... presence of one or more enterotoxin genes [15] Identification of S aureus strains in the form of the species and/ or subspecies, as well as on the percentage of base sequence matching of the tested ... cycles of 95°C for 30 s, 55°C for 30 s, and 72°C for 468 Unauthenticated Download Date | 3/6/17 12:36 AM Pajić et al.: Prevalence and molecular characterization of enterotoxin-producing strains of...

Ngày tải lên: 04/12/2022, 16:02

... was recorded between the length of leaves and length of leaf petiole (0.98), the width of seeds and weight of seeds (0.96), and the width of leaves and length of leaflets (0.94) The dissimilarity ... Department of Food Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia Department of Biology, Faculty of Sciences and Mathematics, University of Split, Split, ... SRECEC, SINISA; and KREMER, DARIO (2021) "Morphological and molecular characterization of Croatian carob tree(Ceratonia siliqua L.) germplasm," Turkish Journal of Agriculture and Forestry: Vol...

Ngày tải lên: 31/01/2023, 20:30

... number of landrace cultivars in their pedigree The genetic variation was subsequently reduced by genetic drift and selection Characterization of genetic diversity of these cultivars by molecular ... for SSR and InDel markers ranged from to and from to (with average number of 4.29 and 2.76), He average was 20.7% and 12.0%, gene diversity average was 0.47 and 0.39, PIC average was 0.40 and 0.32, ... broadening genetic basis of traditional landraces and cultivars in China Furthermore, the genetic diversity and PIC of improved sesame cultivars was found to be lower than those of landraces, especially...

Ngày tải lên: 27/03/2023, 03:41

... prominently in the evolution of these viruses Page of 10 Results Identification, detection and molecular characterization of novel monopartite begomoviruses Leaves of infected tomato plants were ... case of bipartite begomoviruses or monopartite begomoviruses having betasatellite molecules When the IR sequence of K3/K5 and CTM isolates was compared and aligned to the published IR sequences of ... a molecular study of the sequences of genomes of different ToLCV isolates collected from the Indian subcontinent in an effort towards identifying and determining the molecular variability of...

Ngày tải lên: 12/08/2014, 02:20

... explanation for detection of an additional CRFR1e1 protein (band 10) with molecular mass of 20 kDa (Fig 2) Proteins with lower molecular mass than expected included band (39 kDa) for CRFR1f, band (34 ... of a band with 53 kDa a Ó FEBS 2004 Characterization of novel CRFR1 isoforms (Eur J Biochem 271) 2825 Fig Levels cAMP accumulation in transiently transfected COS cells with different CRFR1 isoforms ... Broad band seen in CRFR1a has an apparent molecular mass of 55–65 kDa and most likely represents glycosylated receptor We note that others have also reported detection of CRFR1 proteins with molecular...

Ngày tải lên: 07/03/2014, 15:20

... reaction products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE As shown in Fig 4, 9-HODE is the main product of the hazelnut ... part of the world production adsorbed by the pastry industry Hazelnut seeds contain reasonable levels of linoleic and linolenic acid (about 10 and 0.2%, respectively, of the total fatty acids) and ... C6 and C9 aldehydes (and the corresponding alcohol) were detected in all the samples and, similarly to the pattern of LOX expression and activity, their levels were higher in stage I seed and...

Ngày tải lên: 21/02/2014, 00:20

... and 2,6-DMP were pH 5.6 and 3.4 UÆmg protein)1 and pH 4.6 and 2.8 UÆmg protein)1, respectively In standard assay mixtures, the velocity of ABTS oxidation was maximal at 45 °C The dependence of ... Enzyme characterization The molecular mass of purified lac1 was estimated by SDS/ PAGE to be 58 kDa and the isoelectric point of the enzyme was 3.7 In addition to ABTS, syringaldazine and 2,6-DMP ... °C Enzyme characterization The molecular mass of purified laccase was determined by SDS/PAGE (15% w/v acrylamide gels) using low molecular mass standards (Bio-Rad) The isoelectric point of the enzyme...

Ngày tải lên: 07/03/2014, 15:20

... patients and seven HPA III patients 2050 Characterization and functional analysis of novel mutations Among the mutations identied in our HPA population, two (i.e p.Q301P and c.707-2delA) were novel ... varies in distinct populations and geographic areas [79] and a number of them have been analyzed and characterized in vitro [10,11] Identication of the mutations and subsequent in vitro expression ... retardation and epilepsy, as well as other neurological abnormalities [3] The severity of PAH deciency is variable and partly depends on the nature of the mutations of the PAH gene Recently, a novel...

Ngày tải lên: 16/03/2014, 01:20

... was 1.8°, the standard deviation of the hydrogen bond energies was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant ... in position With the exception of four a-helices and two pairs of short b-strands, the rest of the protein consists almost entirely of loops connecting the b-strands The loops extending from the ... Denaturation and refolding of reCBH and enzyme assay reCBH (1 mg) was denatured by incubation in a solution of M urea/0.1 M Tris/HCl, pH 8.0, in the presence of 100 mM dithiothreitol and mM EDTA,...

Ngày tải lên: 23/03/2014, 13:20

... 50% of its activity in the absence of NaTDC The maximal PLA2 specific activity of 400 UÆmg)1 was measured at pH 9.5 and at 37 °C in the presence of mm NaTDC and mm CaCl2 Pure ChPLA2 lost  80% of ... 4547 A Karray et al A effect of bile salts on ChPLA2 activity, the rate of hydrolysis of PC by ChPLA2 in the presence of various concentrations of bile salts, at pH 9.5 and at 37 °C, was studied ... Lys56; and aD, Ala90–Lys108); (e) two short strands 4550 of an antiparallel b-sheet (b-wing) (b2, Ser74–Ser78; and b3, Glu81–Asn85); and (f) a C-terminal loop containing a short b4-strand (His115–Asn117)...

Ngày tải lên: 30/03/2014, 01:20

... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene ... used to study the efficacy of FTA technology for sampling, retrieval and molecular analysis of these viruses Geminiviruses are composed of monopartite or bipartite genomes of ssDNA, 2.7–2.8 kb in ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C...

Ngày tải lên: 19/06/2014, 08:20

... bring to sampling and molecular study of viral crop diseases, we tested the efficacy of FTA for retrieval of viral pathogens from infected leaf tissues and for the detection of viralderived transgene ... used to study the efficacy of FTA technology for sampling, retrieval and molecular analysis of these viruses Geminiviruses are composed of monopartite or bipartite genomes of ssDNA, 2.7–2.8 kb in ... consisted of 30 cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C...

Ngày tải lên: 20/06/2014, 04:20

... performance of the sows sired by two of these boars only (cases and 6) The boar carrier of the 2/6 translocation sired 51 litters in two herds, with an average litter size of 8.30 The boar carrier of ... intensification of the control programmes, especially in France [10], but also by the improvement of the laboratory techniques used [27] G and/ or R banding is presently used systematically and allows the detection ... detection of rearrangements modifying very slightly the size and/ or the banding profiles of the chromosomes, as, for instance, in the case of the translocations rcp(15;17)(q24;q21) and rcp(7;8)(q24;p23)...

Ngày tải lên: 14/08/2014, 13:21