characterization of nonstructural protein 5b

... with the amount of GmPDIL-3a mRNA, although the amount of GmPDIL-3b mRNA was not consistent with the amount of GmPDIL-3b protein expression (Fig 5B) Expression of many ER-resident proteins can be ... transport of ER proteins, or regulation of ER calcium channel activity [9–12] In plants, a set of 22 orthologs of known PDI family proteins was discovered by a genome-wide search of Arabidopsis ... isolation of membrane protein complexes in enzymatically active form Anal Biochem 199, 223–231 Walker KW, Lyles MM & Gilbert HF (1996) Catalysis of oxidative protein folding by mutants of protein...

Ngày tải lên: 18/02/2014, 11:20

... present the mesoscopic model of biological protein materials composed of protein crystals prescribed by Go potential for characterization of elastic behavior of protein materials Specifically, ... mechanical characterization of protein materials may allow for gaining insight into the biological functions of mechanical proteins Mechanical characterization of biological molecules such as proteins ... Fig Schematic illustration of biological protein materials composed of protein crystals (a) cartoon of a fiber, made of protein crystals, under mechanical loading (b) protein crystal lattices constituting...

Ngày tải lên: 22/02/2014, 08:20

... property of amyloidogenic oligomers, at least in cell culture Studies of the above-mentioned proteins and of other proteins linked to human amyloidoses, including lysozyme [11] and prion protein ... elucidate the structure and properties of proteins has a long history, and techniques for protein- structure characterization cover a very wide range of techniques As proteins in native and other thermodynamically ... Spectroscopy of amyloid oligomers M Lindgren and P Hammarstrom ¨ assessments of structure The situation is, in some respects, similar to the situation of studying protein folding and dynamic protein protein...

Ngày tải lên: 06/03/2014, 09:22

... in LB medium after h of IPTG induction (Fig 1) Purification and refolding of Rv1399c Given the large quantity of insoluble material obtained [up to 160 mg of protein from L of cell culture (Table ... expression of Rv1399c The Rv1399c gene product encodes a 318 amino acid protein with a molecular mass of  31.7 kDa and a calculated pI of 4.53 and belongs to the Lip family that consists of 21 members ... for a monomeric protein (data not shown) 116 kDa 66 kDa 45 kDa Biochemical characterizations of Rv1399c 35 kDa 25 kDa Fig SDS/PAGE for expression and refolding of Rv1399c in E coli Protein samples...

Ngày tải lên: 07/03/2014, 16:20

... quality factor of cantilever and phase of the cantilever relative to the driver [29] The wear rate ranges between Page of 10 Figure Wear of protein layers in tapping mode AFM AFM image of FBS layer ... employed for characterization of amplitude variations of topography and phase across the surface RMS values were also used as an error bar of determined layer thickness [8] Page of 10 Results ... previous reports [30,31] The slope of linear regression of the data is 2.1 × 10-3 scan-1 It should be noted that this is not wear of individual protein molecules but of the layer adsorbed as a whole...

Ngày tải lên: 21/06/2014, 04:20

... approaches to the derivatization of CdSe/ZnS QDs to achieve conjugation of active agonists of the A2AAR The problems of limited aqueous solubility of the QD [20-23] and access of the flexible tethered ... (20 μg of protein) , 50 μL of agonist radioligand, and 50 μL of increasing concentrations of the test ligands in Tris-HCl buffer (50 mM, pH 7.5) containing 10 mM MgCl2 The concentration of the ... conjugates of quantum dots for characterization of G protein- coupled receptors: strategies for immobilizing A2A adenosine receptor agonists Journal of Nanobiotechnology 2010, 8:11 Page 19 of 19 ...

Ngày tải lên: 11/08/2014, 00:22

... glycoprotein I, a protein localized in the transGolgi network Embo J 1996, 15:6096-6110 Alconada A, Bauer U, Sodeik B, Hoflack B: Intracellular Traffic of Herpes Simplex Virus Glycoprotein gE: Characterization ... 5, the pET-32a(+) after induction; C Effect of different time of the pET32a/DPV-gE protein in Rosseta M: Protein marker; Lane 1-6, the pET32a/DPV-gE protein was expressed respectively for 2, 3, ... detected the product of the gE gene Intracellular localization of the gE product in DPV-infected cells To confirm the intracellular localization of gE protein, indirect immunofluorescence studies...

Ngày tải lên: 12/08/2014, 04:20

... total of 11 paralogous protein families with more than one hundred member proteins were identified in rice which encoded proteins such as zinc finger proteins, protein kinases, Myb-like proteins, ... sion (r) of of paralogous protein families with exactly two HistogramricePearson's Correlation Coefficients of expresHistogram of Pearson's Correlation Coefficients of expression (r) of rice paralogous ... 171 147 109 87 78 >8 Number of Proteins in Each Family Figure Arabidopsis Size distribution of paralogous protein families in rice and Size distribution of paralogous protein families in rice and...

Ngày tải lên: 12/08/2014, 05:20

... presentations of apoCaM and Ca2+/CaM 20 Fig 1.9 Summary of CaM binding proteins 22 Fig 1.10 Comparison of the in vivo (with PTMs) and in vitro (without PTMs) states 23 Fig 1.11 A list of protein interaction ... Purification profile of Cbl-TKB 49 Fig 2.2 Gel filtration profile of cleaved Cbl-TKB 49 Fig 2.3 Glutaraldehyde cross-linking of Cbl-TKB 50 Fig 2.4 Initial crystals from the screening of Cbl-TKB complexed ... N Number of binding sites Nav Voltage gated sodium channel isoform Nav1.4IQ IQ motif of voltage gated sodium channel isoform 1.4 Nav1.6IQ IQ motif of voltage gated sodium channel isoform 1.6...

Ngày tải lên: 11/09/2015, 09:11

... isolation of potential interacting protein partners for your protein of interest The knowledge requirement for your protein of interest is only very minimal If a protein has a known function, new proteins ... Co-localization of EV71 VP1 with KIAA0697 Page 80 17 4.1.9: Specific detection of wild-type VP1 protein in EV71-infected cells 81 18 4.1.10: Kinetics of co-localization of wild-type VP1 protein with ... of human herpesvirus (HHV-6) and human herpesvirus (HHV-7) 2.3.1.2.2 Schematic diagram of human herpesvirus oriLyt 39 41 2.3.1.3.1 Alignment of the protein sequences of the serine proteases of...

Ngày tải lên: 12/09/2015, 08:19

... inhibition Ribbon diagrams of the second and third kuniz domain of TFPI, Mechanism of action of TFPI, Ribbon diagram of the minimized mean structures of NAPc2, Mechanism of action of rNAPc2 The predicted ... activation of protein C Activated protein C counteracts coagulation by cleaving and inhibiting the cofactors FVa and FVIIIa The free form of protein S in blood serves as cofactor to activated protein ... Preliminary characterization of the complex using gel-filtration chromatography 87 Chapter Three Mechanism of Anticoagulant Activity vi Identification of the site of action of the anticoagulant protein...

Ngày tải lên: 14/09/2015, 13:36

... facilities to carry out the research work i Table of Contents Acknowledgement Table of Contents Summary List of Tables List of Figures List of Equations List of Symbols and Abbreviations Chapter Introduction ... applications of SPR biosensors have been developed very fast for the investigation of specific biomolecular interactions including protein- antibody interaction, protein- DNA interaction, protein- cell ... First of all, I would like to express my sincere gratitude to my supervisors, Associate Professor Loh Kian Ping from National University of Singapore (NUS) and Dr Su Xiaodi from Institute of Materials...

Ngày tải lên: 03/10/2015, 20:32

... presentation ii Table of contents iii Summary viii List of tables x List of figures xi List of abbreviations xiii Introduction 1 Molecular characterization of CD157 1.1 Identification of CD157 1.2 Cellular ... Construction and characterization of the bait protein 51 2.4.2 Library screening 58 2.4.3 Confirmation of positive interactions 60 Chapter Three: Characterization of the CD157 interacting proteins through ... distribution of CD157 1.3 Genomic structure of CD157 Biological function of CD157 2.1 Pathophysiological roles of CD157 2.2 Cellular functions of CD157 2.3 Enzymatic activities of CD157 2.4 Signaling...

Ngày tải lên: 22/10/2015, 21:20

... screening, offering multi-core capability, high performance and enhanced accuracy and ease of use The server automatically docked the ligands in the calculated binding CHARACTERIZATION OF G PROTEIN ... the best docked configurations of the ligands The best complexes mostly CHARACTERIZATION OF G PROTEIN COUPLED RECEPTORS 47 Fig The lowest Autodock VINA affinity of docked ligands generated by ... description of the putative binding cavities of the latter In mammalian systems, however, ligands are known for fewer than 90 of over 1400 human and mouse ORs, greatly limiting our understanding of olfactory...

Ngày tải lên: 31/10/2015, 10:39

... This hypothesis is supported by the action of NS1 proteins from similar parvoviruses The nonstructural proteins from the parvoviruses minute virus of mouse (MVM) and H-1 parvovirus also utilize ... neighboring proteins in response to DNA damage 92 (36-38) As a method of investigating the involvement of PARP activation in NS1-induced apoptosis, the NS1 fusion protein was examined for the presence of ... the finding of NS1-DNA attachment concerns the development of autoantibodies after B19 infection B19 infection often results in the production of anti-DNA autoantibodies (3, 4) A viral protein, ...

Ngày tải lên: 25/10/2012, 11:18

... of the 4p orbital with the 3d orbital of the metal cofactor Two important pieces of information can be gleaned from the pre-edge peak First, occurrence of this transition implies distortion of ... experiments Protein- bound iron FereneS was added in a final concentration of 20 mm to the protein sample to give a total volume of 600 lL and a protein concentration in the range 5–10 lm The formation of ... superoxide in the course of the reaction The principle of the assay is that the presence of a SOD inhibits the reduction of cytochrome c This was validated by adding a total of 2.5 U of commercial SOD...

Ngày tải lên: 18/02/2014, 06:20

... region of VpU, as indicated by a small number of observed long-range NOEs [24] NMR structure of micelle-associated HIV-1 VpUcyt Another important aspect is the topology of the cytoplasmic domain of ... the expected number of cross-peaks of VpUcyt were obtained with perdeuterated DPC (DPC-d38) at 30 °C A series of HSQC spectra of mm VpUcyt was recorded with varying amounts of DPC-d38 in the sample ... mark the theoretical average values of the downfield shift of 13Ca (top) and 13CO (bottom) as well as of the upfield shift of 1Ha resonances (middle) characteristic of a 100% helical secondary structure...

Ngày tải lên: 18/02/2014, 06:20

... et al COFRADIC and protein modifications analysis of purine-binding proteins in a total lysate of human Jurkat T-cells [40] primary separation mAU 1400 COFRADIC analysis of protein processing ... COFRADIC procedure was applied to a trypsin digest of 10 lL of mouse serum depleted for its three most abundant proteins (albumin, IgGs and transferrin), and resulted in the characterization of ... of ATP-binding proteins As expected, the majority of FSBA-labeled Jurkat proteins were known binders of small nucleotides, cofactors, or DNA and RNA molecules However, several proteins and sites...

Ngày tải lên: 18/02/2014, 16:20

... EI (enzyme I of the PTS), the rst protein in the cascade of proteins forming the PTS, to HPr, the histidine-phosphocarrier protein HPr is the smallest protein in the protein cascade of the PTS ... stability of a protein In Protein Structure (Creighton, T.E., ed.), 2nd edn, pp 253259 Oxford University Press, Oxford 35 Privalov, P.L (1979) Stability of proteins: small globular proteins Adv Protein ... presence of urea Biochemistry 34, 67956804 53 Privalov, P.L & Makhatadze, G.I (1990) Heat capacity of proteins II Partial molar heat capacity of the unfolded polypeptide chain of proteins: protein...

Ngày tải lên: 19/02/2014, 12:20

... reveals the protein regularly accumulating over an expression period of days, while only traces of other proteins were detected After dialysis of culture supernatant, the recombinant protein was ... analysis of proteins was performed by automated Edman degradation using a Perkin-Elmer Procise 494-HT protein sequencer with reagents and methods of the manufacturer Oligomerization of the undenatured ... the mature protein, assuming the formation of two disulfide bridges, was 12 756.6 Da, in agreement with the measured molar mass (12 758.3 ± 1.7 Da) of the native protein [20] This protein does...

Ngày tải lên: 21/02/2014, 03:20