Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system

... GENETIC ENGINEERING AND SURFACE MODIFICATION OF BACULOVIRUS DERIVED VECTORS FOR IMPROVED GENE DELIVERY TO THE CENTRAL NERVOUS SYSTEM YANG YI (B Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... Chapter Three: Genetic Engineering of Baculovirus Vectors for Controlled Gene Delivery ………65 3.1 Introduction………………………………………………………………… 66 3....

Ngày tải lên: 14/09/2015, 11:28

... overview of the progress made by using different types of promoters and vectors for gene delivery to the CNS 1.1 Current progress in gene therapy Gene therapy, which usually refers to somatic gene therapy, ... by the selection of promoters The gene delivery vector that carries and transfers the gene into the nuclei of target cell is another...

Ngày tải lên: 16/09/2015, 08:30

... Packaging of amplicon viral vectors 3.3.10 Analysis of cell cycle-dependent transgene expression in pC8-36 amplicon viral vectors 3.3.11 Effect of transduction of viral vector on the cell cycle profile ... (Castro et al., 2003a) 1.3.2 Gene therapy of gliomas One of the formidable tasks of intracranial gene delivery is the difficulty in achieving gene delivery...

Ngày tải lên: 16/09/2015, 15:54

... Animalia kingdom, Chordata phylum, Vertebrata subphylum, the Reptilia class and Squamata order (Evans, 2003) Venomous Snakes There are many different and diverse species of animals across all phyla ... Cobras, coral snakes, kraits, mambas, sea snakes, sea kraits and Australian elapids Viperidae (viperids) True vipers and pit vipers, including rattlesnakes Table Snakes that known to cause...

Ngày tải lên: 16/10/2015, 15:35

... cells in defined lesional stages Quantification of CCR1, CCR2 and CCR5 mRNA expressing Quantification of CCR1, CCR2 and CCR5 mRNA expressing cells in defined lesional stages Mean numbers of CCR1+ ... expression of CCR1, CCR2 and CCR5 in the spinal cord (data not shown) Enhanced expression of CCR1, CCR2 and CCR5 mRNA was subsequently observ...

Ngày tải lên: 19/06/2014, 22:20

... counterstained with hematoxylin Magnification A and B, ×66 C and D, ×132 Immunohistochmical study of nitric oxide synthase in the central nervous system of goat Enhanced expression of nNOS and iNOS in ... is the first study of NOS expression in the goat brain with listeriosis The involvement of iNOS in bovine and caprine listeriosis h...

Ngày tải lên: 07/08/2014, 14:23

... Primary angiitis of the central nervous system: serial MRI of brain and spinal cord Neuroradiology 2001, 43:599-607 14 Moore PM: Diagnosis and management of isolated angiitis of the central nervous ... Neuropsychopharmacol Biol Psychiatry 2005, 29:627-631 Carandang C, Grant A: Delirium and Isolated Angiitis of the Central Nervous System: a Case...

Ngày tải lên: 09/08/2014, 02:20

... vulnerable, population of patients Chemotherapy- induced cognitive decline in the absence of radiation The best data on the cognitive effects of chemotherapy alone have come from studies of children with ... high doses of chemotherapy is clearly demonstrated by Noble and colleagues [1], yet these proposed studies not take into consideration the possible effects o...

Ngày tải lên: 06/08/2014, 18:21

... sections of CC displayed scattered foci of demyelinated axons, including partial or complete loss of myelin sheaths and increases in inter-laminar splitting of the myelin sheaths Analysis of transverse ... partially destructed myelin sheaths; single arrows indicate inter-laminar splitting of the myelin sheaths; and double arrows indicate myelin debris (b) Two axons...

Ngày tải lên: 06/08/2014, 18:21

... by the nAuNPs The XPS was used to verify the presence of nAuNPs embedded in the brain tissue The confocal microscope and TEM were used to gain insights into the interaction of nAuNPs and the insect’s ... analysis The hyperspectral imaging system from CitoViva was used to identify the location of the nAuNPs particles in the tested roach This imaging system i...

Ngày tải lên: 11/08/2014, 00:22

... intact human saphenous vein ex vivo, the ultimate clinical target http://www.gvt-journal.com/content/2/1/14 HSVEC were obtained by enzymatic collagenase digestion of human saphenous vein and maintained ... with (A) HSVEC and (B) HSVSMC for different times and gene expression quantified and normalised to protein Representative fluorescent images are shown (C) Intact hu...

Ngày tải lên: 14/08/2014, 19:22

... THE ROLE OF HYDROGEN SULFIDE IN THE CENTRAL NERVOUS SYSTEM: IMPLICATIONS IN TREATMENT OF ALZHEIMER’S DISEASE BHUSHAN VIJAY NAGPURE (M.B.B.S., Maharashtra University of Health Sciences, India) ... lot of efforts have been put into the development of disease- modifying drug therapy Amyloidogenesis is one of the main culprits of AD pathology The effe...

Ngày tải lên: 09/09/2015, 08:17

... PTU 1- phenyl-2-thiourea RACE Rapid amplification of cDNA End Rag1 Recombination activating gene Rag2 Recombination activating gene RAS Rat Sarcoma RGC Retina gaglion cell RING Really interesting ... about the neuronal function of Rag1 17 1. 3 Advantages of using zebrafish 18 1. 3 .1 Zebrafish as a model for developmental and genetic research in vertebrates...

Ngày tải lên: 15/09/2015, 17:09

... Summary The objective of this study is to apply biophysical fluorescence technique, i.e fluorescence correlation spectroscopy (FCS), in situ in the central nervous system of fruit fly (Drosophila melanogaster) ... neuronal membrane fluidity was being studied in situ in the central nervous system of Drosophila melanogaster embryos by FCS Our...

Ngày tải lên: 12/10/2015, 17:36

... surface roughness and evaluated mechanical properties of ZnO thin films and bulk materials (S.-R Jian* et al.) ZnO fiilm/sapphire ZnO film/c-sapphire ZnO films/(0001) 6H-SiC ZnO films ZnO (bulk) 4.1 ... properties of thin films/substrates systems Experimental Details The Growth Conditions of ZnO Thin Films ZnO thin films were grown on (0001) (c-oriented), ð1120Þ...

Ngày tải lên: 22/06/2014, 01:20