P125, a COPII INTERACTING PROTEIN 1
P125, a COPII INTERACTING PROTEIN 1
... Sar1p (Gimeno et al., 19 95; SaitoNakano and Nakano, 2000) 1. 7 The COPII subunit proteins 1. 7 .1 Sar1- the small GTPase Sar1 (supressor of activated ras1) is a small G -protein of the Ras superfamily ... loading with GTP, Sar1 (Sar1-GTP) is activated and is recruited to the ER membranes (Nakano and Muramatsu, 19 89) Sar1 activation is facilitated by Sec12, an ER-resident guanine n...
Ngày tải lên: 14/09/2015, 08:42
P125, a COPII INTERACTING PROTEIN 2
... 5’-GACAGCGGCCGCTTCAATTCAAATCTTTTTGATTAGACAG-3’ p 12 5A- 650-R 5’-CAAGGCGGCCGCTTCACTGCTTCACAACTCCATTAGCAAC-3’ p 12 5A- 20 0-F 5’-CGGAGAGTCGACCCTGCAGCAGTGCCAAACACCAGGC-3’ p 12 5A- 26 0-F 5’-GTCCGAGTCGACCCTACTTCAAAACCAATATGAGCCTG-3’ p 12 5A- CT100-F ... TCGAGAAAAAGGAACATCTGCTCAGCAATTCTCTTGAAATTGCTGAGCAGATGTTCCGGG -3’ p 12 5A- PSRP-F 5’- GATCCCCGTTGACCATTTGGTGTTTGTTCAAGAGACAAACACCAAATGGTCA...
Ngày tải lên: 14/09/2015, 08:42
P125, a COPII INTERACTING PROTEIN 3
... co-immunoprecipitate Sec3 1A and vice versa However, both the available anti-Sec3 1A and anti-p12 5A antibodies 56 Thyroglobulin 669 kDa Apoferritin 4 43 kDa 150 kDa WB : Sec3 1A 150 kDa 100 kDa WB : p12 5A 75 kDa WB ... Sec 13 siRNA Bar = 10 μM p12 5A B Non-target siRNA Se ec3 1A siRNA Non-target siRNA Sec 13 siRNA A 83 84 Sec3 1A siRNA A p12 5A siRN NA Non-Target siRNA GT-G...
Ngày tải lên: 14/09/2015, 08:42
P125, a COPII INTERACTING PROTEIN 4
... of the COPII coat can link to the inner layer (Bi et al., 2007) As p12 5A can bind to both Sec2 3A (Tani et al., 1999; Nakajima et al., 2002; Shimoi et al., 2005) and Sec3 1A, and have the ability ... p12 5A or KIAA0310p also did not induce a similar effect on the ERES Depletion of KIAA0310p abolished almost all the punctate staining of ERES markers (Watson et al., 2006; Bhattacharyya...
Ngày tải lên: 14/09/2015, 08:42
P125, a COPII INTERACTING PROTEIN 5
... the phagophore, and is required for the formation of autophagosomes (Nagatogawa et al., 2007) Atg8-PE is postulated to be a scaffold protein that supports membrane expansion and also have functions ... regulate p12 5A s protein interactions and stability of interactions in a metal ion dependent manner similar to ALG-2 5. 3 The Sterile Alpha Motif The SAM (Sterile Alpha Motif) domain...
Ngày tải lên: 14/09/2015, 08:42
P125, a COPII INTERACTING PROTEIN 6
... 71 Iinuma, T., Aoki, T., Arasaki, K., Hirose, H., Yamamoto, A. , Samata, R., Hauri, H.P., Arimitsu, N., Tagaya, M., and Tani, K (2009) Role of syntaxin 18 in the organization of endoplasmic reticulum ... subdomains J Cell Sci 122, 168 0- 169 0 72 Iinuma, T., Shiga, A. , Nakamoto, K., O'Brien, M.B., Aridor, M., Arimitsu, N., Tagaya, M., and Tani, K (2007) Mammalian Sec 16/ p250 plays a rol...
Ngày tải lên: 14/09/2015, 08:42
Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx
... I 13 98 + 14 62 11 22 13 05 10 13 + 13 98 13 05 11 22 13 98 14 62 14 62 10 13 11 22 13 05 13 98 14 62 + 14 62 13 98 11 22 11 22 10 13 13 05 62 13 98 14 62 13 05 13 98 11 22 13 05 62 13 98 13 05 III 11 22 10 13 IV AD1 ? ? Repression ... FEBS 217 9 Autoregulation of GRIP1 functions via C-terminal region P.-Y Liu et al 11 22 13 05 10 13 13 98 14 62 11...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Huntington’s disease: roles of huntingtin-interacting protein 1 (HIP-1) and its molecular partner HIPPI in the regulation of apoptosis and transcription pptx
... Htt-interacting protein HIP -1 and its molecular partner HIPPI in the regulation of apoptosis and transcription, the two processes that are altered in HD [7,8] HIP -1 – its interacting partners and ... Conclusions HIP -1 and its interacting partner HIPPI together induce apoptosis by the intrinsic and extrinsic pathways Homer 1c, an interactor...
Ngày tải lên: 23/03/2014, 07:20
Isolation and characterization of aurora a kinase interacting protein (AKIP), a novel negative regulator for aurora a kinase
... Hypothesis of Possible Anti-Tumour Role of AKIP-mediated UbIndependent Degradation of Aurora- A, 234 ix Abbrevations aa A Box AD ADH AIK1 AKIP ALLM ALLN AML Amp APC/C ATP AURKA AZ AZI amino acid Aurora ... yeast and successfully isolated a novel negative regulator of Aurora- A kinase, named as AKIP (Aurora- A Kinase Interacting Protein) AKIP is an ubiquitous...
Ngày tải lên: 14/09/2015, 22:15
Identification and characterization of protein kinase CK2 as a novel interacting protein of neuronal CDK5 kinase and its functional role in microtubule dynamics
... acidic proteins (Hathaway and Traugh, 1982) Two distinct casein kinases have been found in many different cell types They have been designated casein kinase (CK1) and casein kinase (CK2) according ... Ca2+/calmodulin-dependent protein kinase II cAMP cyclic adenosine monophosphate Cdk cyclin-dependent kinase Cdk5 cyclin-dependent kinase cDNA complementary deoxyribonucleic a...
Ngày tải lên: 16/09/2015, 17:10
Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... Allowing two substitutions Allowing two substitutions Allowing one substitution AAAGACATG AAAGACAGG AAAGAGATT AAAGAGATG AAAGACATA AAAGACATA AAAGAGAAC AAAGACATA AAAGAAAAG AAACAGATG AAAGAAAAG AAAGAAAAG ... EMSA Result Average Kd (nM) AAAGACATG AGAGACATG AAGGACATG AAATACATG AAAGCCATG AAAGAGATG AAAGACCTG AAAGACACG AAAGACATA + ND ND – – + ND ND ND + – – – – + – + – 0.25 1.5 p=0.01 60_HI 60M_HI Fig...
Ngày tải lên: 07/03/2014, 10:20
Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
... ⁄ Akt signaling was not necessary for EGFinduced protection of TMK -1 cells against apoptosis Role of AP1 in EGF-mediated cell protection A B C MAP kinase signaling provides an alternative pathway ... induced by DNA-damaging agents Oncogene 17 , 13 1 14 1 15 Geier A, Beery R, Haimsohn M, Hemi R, Malik Z & Karasik A (19 94) Epidermal growth factor, phorbol e...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... we have purified, cloned and characterized Cyn d 24 as a novel pathogenesis-related protein from BGP Additionally, the identification of Cyn d 24 has identified the involvement of a novel class of ... AGAD AADA.NA.VG D. D 113 Zea AYA.S A- QRQG LI GG FW AGAD.SASDA.GS.VS QY.DHDT.S 112 Nicotiana AYA.N S-Q.AA NL HGQ AE -GDFMTAAKA.EM.V QY.DHD 118 Cyn d 24 DQGKM...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx
... requires protein translocation to the nucleus Although functional analyses of individual domains have not been addressed, the global context of the domain analyses allows us to draw a more general ... 0.75 are shown Black geometrical shapes are additional domains, as indicated ANOGA, Anopheles gambiae; ASHGOS, Ashbya gossypii; BRARE, Brachydanio rerio; CANDGLA, Candi...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: The proapoptotic member of the Bcl-2 family Bcl-2 / E1B-19K-interacting protein 3 is a mediator of caspase-independent neuronal death in excitotoxicity pot
... protein (BNIP3) is a member of a unique subfamily of death- inducing mitochondrial proteins [14,15] BNIP3-induced cell death has been characterized by early plasma membrane and mitochondrial damage ... glutamate-induced excitotoxicity BNIP3 is a BH3-only proapoptotic member of the Bcl-2 family However, unlike in other members of the Bcl-2 family, t...
Ngày tải lên: 22/03/2014, 17:20