cytoplasmic fmr1 interacting protein 1 and other downstream fmrp targets
Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx
... 13 05 13 98 14 62 14 62 14 62 13 98 11 22 10 13 II 13 05 11 22 13 05 I 13 98 + 14 62 11 22 13 05 10 13 + 13 98 13 05 11 22 13 98 14 62 14 62 10 13 11 22 13 05 13 98 14 62 + 14 62 13 98 11 22 11 22 10 13 13 05 62 13 98 14 62 13 05 ... GST 11 22 -13 04 13 05 -13 98 13 05 -14 62 10 13 99 -14 62 11 22 -14 62 GST-GRIP1 GST GST-GRIP1 Input 10 % B GRIP1 11 22 -14 62 GST-GRIP 113 05 -13 98 GRIP1 GST Input 10 % C 14 62 765 563 11 21 112 2 14 62 Fig GRIP1 forms ... 10 x AD1 11 22 Luciferase Activity B (RLU 10 ) 12 0 [Gal4DBD; pM vector] 563 11 21 AD1 10 13 AD1 Mr 14 62 AD2 10 16 0 1x 10 13 11 21 AD1 C 40 1x [Gal4DBD; pM vector] 35 858x 29000x Mr 10 55 40 10 0 72...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1 doc
... Biol 16 7, 11 1 12 2 Krugmann S, Andrews S, Stephens L & Hawkins PT (2006) ARAP3 is essential for formation of lamellipo- 15 17 18 19 20 21 dia after growth factor stimulation J Cell Sci 11 9, 425–432 ... thio-b-d-galactopyranoside and grown for an additional 16 h at 18 °C His-tagged Rap1, His-tagged Krit1-Nter WT and APAA, GST-HypoKrit1, GST-HypoKrit1DC, Krit1Nter-GST, and GST-ICAP -1 were produced in E coli Bl 21 gold ... binding of 10 lM Rap1GTPcS to lM GST-HypoKrit1 in the absence or presence of 10 lM Krit1-Nter and 10 lM ICAP -1 Rap1 and Krit1-Nter were detected by immunoblotting using anti-His serum ICAP -1 was revealed...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: Huntington’s disease: roles of huntingtin-interacting protein 1 (HIP-1) and its molecular partner HIPPI in the regulation of apoptosis and transcription pptx
... partners of HIP -1 with known functions and results with knockout mice Fig Various domains of HIP -1 The ANTH ⁄ ENTH domain (38 16 0), coiled-coil domain (3 71 610 ), and talin-like domain ( 814 11 12) were ... 28, 425–433 10 Li SH & Li XJ (2004) Huntingtin protein interactions and the pathogenesis of Huntington’s disease Trends Genet 20, 14 6 15 4 11 Li XJ, Friedman M & Li S (2007) Interacting proteins ... [ 21] Depending on the status of phosphorylation of HIP -1 by Dyrk1, HIP -1 interacts with caspase-3 and enhances apoptosis, in a condition where interaction and phosphorylation of HIP -1 by Dyrk1...
Ngày tải lên: 23/03/2014, 07:20
báo cáo hóa học: " Apolipoprotein E expression is elevated by interleukin 1 and other interleukin 1-induced factors" ppt
... interactions of apolipoprotein E with microtubule-associated protein tau: implications for Alzheimer disease Proc Natl Acad Sci U S A 19 94, 91: 111 8 311 186 Bu G: Apolipoprotein E and its receptors in ... Foundation, and the Donald W Reynolds Foundation 13 REFERENCES: 10 11 12 13 14 15 16 Blackman JA, Worley G, Strittmatter WJ: Apolipoprotein E and brain injury: implications for children Dev Med ... Apolipoprotein E expression is elevated by interleukin and other interleukin 1- induced factors Ling Liu1; Orwa Aboud1; Richard A Jones1; Robert E Mrak4; W Sue T Griffin1-3*; Steven W Barger1-3 Department...
Ngày tải lên: 19/06/2014, 22:20
Báo cáo y học: " The cell biology of HIV-1 and other retroviruses" pptx
... Page of 10 (page number not for citation purposes) Retrovirology 2006, 3:77 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Jolly C, Kashefi K, Hollinshead M, Sattentau QJ: HIV -1 cell-to-cell ... apparatus that associates with Tsg1 01 In addition to the ESCRT -1 components Vps28 and Vps37, Tsg1 01 also binds Hrs, Alix, the GGA proteins, and TOM1L1 Interestingly, TOM1L1 also interacts with Nedd4-like ... Natl Acad Sci USA 2006, 10 3 :11 364 -11 369 Kjems J, Askjaer P: Rev protein and its cellular partners Adv Pharmacol 2000, 48:2 51- 298 Basyuk E, Boulon S, Skou Pedersen F, Bertrand E, Vestergaard Rasmussen...
Ngày tải lên: 13/08/2014, 09:20
Characterisation of agrobacterium vird2 interacting protein DIP and its homologues
... and VirE 1. 2.2 .1 Formation of T-complex 1. 2.2.2 Nuclear localization of T-complex 11 1. 2.2.3 Integration of T-DNA 17 1. 2.3 VirB and VirD4, a type IV secretion system (T4SS) 18 1. 2.4 VirF 25 1. 2.5 ... Interference 3 .1 Introduction 79 79 iii 3 .1. 1 General overview of RNA interference 80 3 .1. 1 .1 Definition and assay of RNA interference 80 3 .1. 1.2 Mechanism of RNA interference 81 3 .1. 1.3 Relation ... for DIP 14 1 Interaction 4 .1 Introduction 14 1 4.2 Materials and methods 14 4 iv 4.2 .1 Construction of VirD2 deletion plasmids and strains 14 4 4.2.2 Yeast two-hybrid analysis 14 4 4.3 Results 14 7 4.4...
Ngày tải lên: 12/09/2015, 09:42
P125, a COPII INTERACTING PROTEIN 1
... by Sar1p (Gimeno et al., 19 95; SaitoNakano and Nakano, 2000) 1. 7 The COPII subunit proteins 1. 7 .1 Sar1- the small GTPase Sar1 (supressor of activated ras1) is a small G -protein of the Ras superfamily ... the B-site, and Sec22 likely binds to Sec24 in a complex manner (Miller et al., 2003) 1. 7.3 Sec13/Sec 31 complex There are two mammalian Sec 31 isoforms, Sec31A ( ~13 5 kDa) and Sec31B ( ~12 9 kDa) (Shugrue ... form (Nakano and Muramatsu, 19 89; Barlowe et al., 19 93) Upon loading with GTP, Sar1 (Sar1-GTP) is activated and is recruited to the ER membranes (Nakano and Muramatsu, 19 89) Sar1 activation is...
Ngày tải lên: 14/09/2015, 08:42
Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx
... Grierson FEBS Journal 278 (2 011 ) 11 26 11 36 ª 2 011 The Authors Journal compilation ª 2 011 FEBS C Forzani et al 10 11 12 13 14 15 16 17 18 CS, Hirt H et al (2004) OXI1 kinase is necessary for oxidative ... (2 011 ) 11 26 11 36 ª 2 011 The Authors Journal compilation ª 2 011 FEBS C Forzani et al PTI1-4, a common target of OXI1 and MAPKs HA-OXI1 HA-OXI1 Col-0 PTI1-4-Myc Col-0 PTI1-4-Myc HA Input OXI1 16 ... two other FEBS Journal 278 (2 011 ) 11 26 11 36 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 11 27 pBD-OXI1 pBD-OXI1 pBD pBD-OXI1 pBD-PTI1-4 pBD pBD-PTI1-4 C Forzani et al pBD GST-tagged proteins...
Ngày tải lên: 14/02/2014, 19:20
Báo cáo khoa học: Interactions of HIPPI, a molecular partner of Huntingtin interacting protein HIP1, with the specific motif present at the putative promoter sequence of the caspase-1, caspase-8 and caspase-10 genes pdf
... 1. 20E-008 1. 28E-008 1. 00E-008 25 50 75 10 0 0 .10 0 0.095 0.090 0.085 1. 26E-008 AAAGACACG Kd=4 nM 0 .10 5 Kd =1. 2 nM 1/ ΔF340 1/ ΔF340 0 .11 0 Kd=0.29 nM 1. 32E-008 AAAGACATG 1. 40E-008 20 25 30 35 40 45 50 1/ [DNA]µM ... 32.5 ± 1. 8 (13 .5-fold) 23 .1 ± 1. 3 (2.5-fold) 90.2 ± 11 .1 (2.2-fold) 10 .8 ± (7.2-fold) P-values GFP–N-terminus of HIPPI (fold) P-values 0.00 01 12.5 ± 3.9 0.3 0.00 01 0.0002 0.0005 17 .6 ± 1. 9 (7.3-fold) ... Casp1ups 60 bp I C1ups 717 bp (HIPPI-pDED) C1ups 717 bp (HIPPI-Nterm) C1ups 60bp (HIPPI-pDED) 0.00 0. 21 II C1ups 60bp (6XHN-HIPPI_pDED) Kd=0.34nM 0.20 1/ ΔF Fluorescence305 B shifted band 0 .19 0 .18 ...
Ngày tải lên: 07/03/2014, 10:20
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... contain- B A S103 G145/D137 Y 112 D2 01/ 193 L75/67 G145/D137 E1 41/ 133 K173 /16 5 R207 /19 9 G77/69 D142 /13 4 G197 /18 9 Fig Superposition of PRTFDC1 with HPRT-ImmGP (Protein Data Bank code: 1BZY) (A) Residues ... Ramachandran plot (%)c Favored regions Additionally allowed regions ID29 1. 04 P3 21 a = b = 13 9.2 c = 52 .1 Two subunits 1. 7 (1. 70 1. 79) 10 0 (10 0) 10 .0 (48.2) 19 .4 (4.2) 10 .8 (11 .0) 63939 18 .2 21. 5 ... human PRTFDC1 B A PRTFDC1 25 HPRT 20 10 00 0.2 800 600 400 0 .1 200 –50 50 10 10 50 10 015 0 200 250 S 200 –50 250 50 12 00 10 0 15 0 [Hx] (µM) 2.0 PRTFDC1 50 10 0 15 0 200 250 S HPRT D C 0.0 12 15 S/V V...
Ngày tải lên: 15/02/2014, 01:20
Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx
... Glu C peptides 83 -10 8 +2P 93 -10 0 +P 93 -10 3 +P 93 -10 3 +2P 93 -10 8 +P 10 0 -11 1 +P 10 9 -12 1 +P 10 9 -12 2 +P 11 2 -12 1 +P 11 3 -12 1 +P 16 1 -17 2 +P Tryptic peptides 16 5 -17 5 +P 16 5 -18 3 +P Sequence Calculated mass ... 16 + 25+ 24+ 22+ 13 + 14 + C 25 410 Da 21+ 20+ 19 + 18 + 23+ 25+ 15 + 17 + 16 + 15 + 13 + 14 + 24+ B 21+ 20+ 19 + 22+ 16 + 18 + 23+ 25+ 17 + 15 + 24+ 14 + 13 + A 10 00 12 00 253 31 Da 14 00 16 00 18 00 252 51 Da 2000 m/z ... 2787 .1 853.4 11 69.4 12 49.4 17 70.7 13 57.6 15 94.7 17 08.7 12 53.5 11 39.4 13 30.0 2787.8 853.2 11 69.3 12 49.2 17 71. 4 13 57.6 15 95.2 17 08.6 12 53.9 11 39.9 13 30.3 +0.7 )0.2 )0 .1 )0.2 )0.3 )0.5 )0 .1 +0.4...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
... activation of a cytosolic protein FEBS Journal 274 (2007) 310 8– 311 9 ª 2007 The Authors Journal compilation ª 2007 FEBS 311 7 ATP binding to IRP -1 10 11 12 13 14 15 16 17 18 19 Z Popovic and D M Templeton ... Collection (Rockville, MD) and grown in a-MEM supplemented with 10 % fetal bovine serum, penicillin (10 0 UÆmL )1) and streptomycin (10 0 lgÆmL )1) Cells FEBS Journal 274 (2007) 310 8– 311 9 ª 2007 The Authors ... Km and Kd values are in reasonable agreement, and it may be that factors other FEBS Journal 274 (2007) 310 8– 311 9 ª 2007 The Authors Journal compilation ª 2007 FEBS 311 3 ATP binding to IRP -1 A...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf
... this protein in metazoan cells Experimental procedures Strains and media Strains W303-1B (mat a, ade2 1, ura3 1, his3 11 , trp1 1, leu2–3 ,11 2, can1 10 0), FYF4-A1 aco1 (mat a, ura3–52, trp1D63, ... fractions Lane 1, recombinant human IRP1; lanes 2– 11 , fractions 1 10 of the sucrose gradient (C) Fraction 10 was diluted in Tris buffer (lane 1) or Tris buffer containing 1% Triton X -10 0 (lane 2) ... Iron–sulfur protein ¨ biogenesis in eukaryotes Trends Biochem Sci 30, 13 3– 14 1 Lauble H & Stout CD (19 95) Steric and conformational features of the aconitase mechanism Proteins 22, 1 11 Dupuy J,...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx
... results CCN4 ⁄ WISP1 and WISP1v proteins were actually translated from CCN4 ⁄ WISP1 and WISP1v mRNAs in HCS-2 ⁄ cells A rabbit ortholog of the reported CCN4/WISP1splicing variant (WISP1v) that is associated ... fulllength CCN4 ⁄ WISP1 mRNA and WISP1v were Finally, the biological function of WISP1v and WISP1vx was evaluated using overexpression experiments FLAG epitope-tagged WISP1v and WISP1vx were overexpressed ... insulin-like growth factor -1 on breast epithelial cells Breast Cancer Res 7, 10 80 10 89 11 Luo X, Ding L & Chegini N (2006) CCNs, fibulin-1C and S100A4 expression in leiomyoma and myometrium: inverse...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: Plasmodium falciparum merozoite surface protein 1 Glycosylation and localization to low-density, detergent-resistant membranes in the parasitized erythrocyte pdf
... Substrate 19 5 kDa MSP -1 86 kDa N-ter 56 kDa C-ter BSA, lg Galactosyl transferase b-galactosidase c.p.m 20 20 20 20 20 20 20 20 20 20 0 10 mU 0 10 mU 0 10 mU 0 283 14 69 13 65 19 8 264 13 54 16 07 264 210 12 63 ... positions 12 71 (Ser), 12 78 (Thr) and 12 83 (Ser) (filled circles) Position 12 71 was positive in the Thai and Wellcome sequences while position 12 83 was positive for the Ghana, Png and Uganda sequences ... (387– 413 and 11 00 11 87) within the sequences of blocks 5 16 of the two dimorphic forms The comparison could not be extended to the a-O-GlcNAc sites because the MSP -1 proteins of Thai-K1 and Wellcome...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: GHP, a new c-type green heme protein from Halochromatium salexigens and other proteobacteria potx
... (2855.2) 16 68.7 10 02.5 10 25.5 12 31. 5 16 12.7 19 43.9 24 01. 1 2289 .1 2746.3 (502.2) 13 21. 7 9 01. 4 8 31. 3 19 89.8 19 41. 9 411 0 .1 39 81. 0 3852.0 2805.8 2676.7 2547.7 213 5.0 29– 41 3–28 2–28 1 28 66–72 42–65 11 –25 ... Cys-SO2H Arg Met b10 b 11 1208.7 12 77.7 10 56 Mass (Da) Cys-SO3H + Glu Gln Asn Leu Glu 12 51. 7 10 77.6 b15 17 31. 2 b16 18 44.3 10 0 b9 b8 9 21. 7 822.6 b13 b13 14 89 .1 b14 b 11 1208.9 % - H2SO2 18 00 - H2SO3 ... Da) from b12 and/ or b13 % B * 12 77.7 Mass (Da) Val Met C b14 16 16.8 b15 17 30.8 * 10 0 b10 10 77.6 b9 b8 9 21. 5 822.5 b10 10 77.9 12 52.0 * D 16 17.2 14 07 .1 * 12 78.0 14 72.7 15 32 800 Mass (Da) 18 00 Fig...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube pptx
... pACT2FEZ1 (Y156), pACT2FEZ1 (Y163), pACT2CIB (Y198) or pGAD424NBR1 and (i) pGBT9NBR1 (ii) pGBT9NBR1 216 (iii) pGBT9NBR1333 (iv) pGBT9NBR1C term (v) pGBT9NBR1c1 (vi) pGBT9NBR1c2 (vii) pGBT9NBR1c3, ... pGBT9NBR1333 and pGBT9NBR1Cterm were able to interact with full-length NBR1 (see Fig 1B, i,iii,iv and vi) FEZ1 and CIB interact with each other To con®rm the interactions between NBR1, CIB and FEZ1, ... con®rm that NBR1 and FEZ1 interact in vivo COS-7 cells (lane 1) , COS-7 cells transfected with pHA-NBR1 (lane 2), pFEZ1-EGFP (lane 3), pHA-NBR1 and pFEZ1-EGFP (lane 4) and pHA-NBR1 and pEGFP-N2...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo khoa học: Retention of the duplicated cellular retinoic acid-binding protein 1 genes (crabp1a and crabp1b) in the zebrafish genome by subfunctionalization of tissue-specific expression doc
... (19 98) Nuclear detection of cellular retinoic acid binding proteins I and 3570 R.-Z Liu et al 15 16 17 18 19 20 21 22 23 24 25 26 27 28 II with new antibodies J Histochem Cytochem 46, 11 03 11 11 ... Genetics 15 1, 15 31 15 45 46 Lynch M & Conery JS (2000) The evolutionary fate and consequences of duplicate genes Science 290, 11 51 11 55 47 Ruberte E, Friederich V, Chambon P & Morriss-Kay G (19 93) ... 60 aa 16 aa 12 22 bp 38 aa 2277 bp 60 aa 511 bp 16 aa 7470 bp 3453 bp 554 bp 23 aa 16 aa 38 aa 6 815 bp 19 59 bp 23 aa Exon 3262 bp 7629 bp 23 aa Intron 38 aa 16 aa 4 313 bp 38 aa 2925 bp 16 aa 4328...
Ngày tải lên: 16/03/2014, 22:20
Báo cáo khoa học: Mixed lineage kinase LZK and antioxidant protein-1 activate NF-jB synergistically docx
... LZK could be dependent on the binding partner, such as JIP -1 and AOP -1 10 11 12 13 14 Acknowledgements We wish to thank Drs E Nishida and K Shimotohno for the generous gifts of the plasmids used ... JNK1: a protein kinase stimulated 15 16 17 by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain Cell 76, 10 25 10 37 Han, J., Lee, J.D., Bibbs, L & Ulevitch, R.J (19 94) ... SEK -1 J Biol Chem 2 71, 19 025 19 028 Tibbles, L.A., Ing, Y.L., Kiefer, F., Chan, J., Iscove, N., Woodgett, J.R & Lassam, N.J (19 96) MLK-3 activates the SAPK/JNK and p38/RK pathways via SEK1 and...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Inhibitors of protein phosphatase 1 and 2A decrease the level of tubulin carboxypeptidase activity associated with microtubules pptx
... 3, 11 41 11 54 Sloboda, R.D., Rudolph, S.A., Rosenbaum, J.L & Greengard, P (19 75) Cyclic AMP-dependent endogenous phosphorylation of a microtubule-associated protein Proc Natl Acad Sci USA 12 , 17 7 18 1 ... J Cell Biol 10 5, 2 51 264 Schulze, E., Asai, D.J., Bulinski, J.C & Kirschner, M (19 87) Posttranslational modification and microtubule stability J Cell Biol 10 5, 216 7– 217 7 Kreis, T.E (19 87) Microtubules ... Bulinski, J.C & DeBrabander, M (19 86) Ultrastructural colocalization of tyrosinated and detyrosinated alpha-tubulin in interphase and mitotic cells J Cell Biol 10 3, 18 83 18 93 ´ 10 Bre, M.H., Kreis,...
Ngày tải lên: 23/03/2014, 15:21