... 0.9 2.9 · 10 6 ± 1. 0 · 10 6 (10 0%) Guanine PRTFDC1 36 .1 ± 14 .3 2.9 ± 0.7 1. 36 ± 0.34 3.9 · 10 4 ± 9.3 · 10 3 (0.09%) HPRT 9.9 ± 0.2 899 ± 11 7 406 ± 53 4.5 · 10 7 ± 1. 0 · 10 7 (10 0%) M. Welin et ... 40 60 80 10 0 12 0 S/V 200 250 [Hx] (µ M ) 0 50 10 0 15 0 200 250 [Hx] (µ M ) [G] (µ M )[G] (µ M ) 0 12 010 0806040200 10 20 30 40 50 60 2.0 12 00 25 20 15 10 5 0 10 00 800 600 400 200 0 1. 5 1. 0 0.5 0.0 Fig. ... this. K m (lM) V max (lmolÆmin )1 Æmg )1 ) k cat (s )1 ) k cat ⁄ K m (s )1 ÆM )1 ) Hypoxanthine PRTFDC1 23.3 ± 6.8 0.340 ± 0.037 0 .16 ± 0.02 7.4 · 10 3 ± 1. 9 · 10 3 (0.26%) HPRT 3.9 ± 1. 5 23.3 ± 2.0 10 .5 ± 0.9 2.9 · 10 6 ±...
Ngày tải lên: 15/02/2014, 01:20
... +86 23 654 616 96 Tel: +86 23 654 616 96 E-mail: yshuang.tmmu@gmail.com (Received 3 December 2009, revised 3 February 2 010 , accepted 11 February 2 010 ) doi :10 .11 11/ j .17 42-4658.2 010 .07 615 .x Tumour ... isoproterenol. Circ Res 50, 10 1 11 6. TRAP1 protects cells from hypoxic injury by MPTP F. Xiang et al. 19 38 FEBS Journal 277 (2 010 ) 19 29 19 38 ª 2 010 The Authors Journal compilation ª 2 010 FEBS directly lead ... units) 0 Normoxia * * * * 13 6 H yp oxia treatment (h) 12 0.4 TRAP1 β-actin 0.3 0.2 0 .1 TRAP1/β-actin 0 Normoxia * * * * 13 6 Hypoxia treatment (h) Hypoxia treatment (h) 12 Normoxia 13 612 75 kD a 43 kD a Fig. 1. Effects...
Ngày tải lên: 16/02/2014, 14:20
Tài liệu Báo cáo khoa học: Olfactory receptor signaling is regulated by the post-synaptic density 95, Drosophila discs large, zona-occludens 1 (PDZ) scaffold multi-PDZ domain protein 1 pptx
... be l MINT-7290 219 :hOR3A1 (uniprotkb:P478 81) binds (MI:0407 )toMUPP1 (uni- protkb: O75970)bypeptide array (MI:00 81) l MINT-729 019 1:hOR1D2 (uniprotkb:P34982) binds (MI:0407)toMUPP1 (uni- protkb: O75970)bypeptide ... MINT-7290063, MINT-729 011 0: OR2AG1 (uni- protkb: Q9H205) binds (MI:0407)toMUPP1 (uniprotkb:O75970)bypeptide array (MI:00 81) l MINT-729 016 2: mOR283 -1 (uniprotkb:Q9D3U9) binds (MI:0407)toMUPP1 (uni- protkb: O75970)bypeptide ... four experiments. Other olfactory receptors, such as mOR167-4, mOR199 -1, M 71, M72 and mOR2 41- 1 A C DE B Fig. 2. The C-terminus of OR2AG1 interacts with MUPP1 in vitro. (A) Pie chart illustrating the abundance...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: Electrostatic contacts in the activator protein-1 coiled coil enhance stability predominantly by decreasing the unfolding rate docx
... Fig. 4. k f1 (M )1 Æs )1 ) m u –m t1 (calÆmol )1 ÆM )1 ) k f2 (s )1 ) m I –m t2 (calÆmol )1 ÆM )1 ) k u1 (s )1 ) m f –m t2 (calÆmol )1 ÆM )1 ) k u2 (s )1 ) m I –m t1 (calÆmol )1 ÆM )1 ) DG kin (kcal Æmol )1 ) cJun–FosW ... ER = )1. 45 ( )1. 5) g 3 )e’ 4 ER = )1. 45 ( )1. 5) ER = )1. 45 ( )1. 5) e 2 )g’ 1 EK = )1. 15 ( )1. 5) RE = )1. 45 ( )1. 5) e 3 )g’ 2 RQ = )0.7 ( )1) RE = )1. 45 ( )1. 5) e 4 )g’ 3 QT = ? (?) RE = )1. 45 ( )1. 5) Total ... k u2 (s )1 ) m I –m t1 (calÆmol )1 ÆM )1 ) DG kin (kcal Æmol )1 ) cJun–FosW 5.8e 6 ± 1. 3e 6 )1. 4 ± 0.2 2.3 ± 0.5 )0.2 ± 0.2 0.046 ± 0. 01 1.0 ± 0 .1 0.92 a 4.2 b ?? cJun(R)–FosW(E) 7.1e 6 ± 1. 6e 6 )1. 9 ± 0.2 4.0 ± 0.7 )1. 0 ± 0 .1 0.00 01 ± 0.00 01 2.5 ± 0.21...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
... Osaka 573- 010 1, Japan Fax: + 81 72 866 311 7 Tel. + 81 72 866 311 8 E-mail: takeuchi@pharm.setsunan.ac.jp (Received 31 March 2006, revised 3 June 2006, accepted 14 June 2006) doi :10 .11 11/ j .17 42-4658.2006.05377.x We ... containing 13 7 mm NaCl, 2 mm EGTA, 5 mm EDTA, 0 .1% Nonidet P-40, 0 .1% Triton X -10 0, 10 0 lgÆmL )1 PMSF, 1 lgÆmL )1 pepstatin A, 1 lgÆmL )1 p-toluenesulfonyl-l-arginine methyl ester, 2 lgÆmL )1 leupep- tin, ... 10 3 11 1. 17 Meisenhelder J, Suh PG, Rhee SG & Hunter T (19 89) Phospholipase C-c is a substrate for the PDGF and EGF receptor protein- tyrosine kinases in vivo and in vitro. Cell 57, 11 09 11 22. 18 ...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
... Yokohama 244–0 813 , Japan Fax: + 81 4582 019 01 Tel: + 81 4582 019 05 E-mail: miyazaki@yokohama-cu.ac.jp (Received 25 August 2005, revised 15 November 2005, accepted 8 December 2005) doi :10 .11 11/ j .17 42-4658.2005.05094.x Insulin-like ... it might be a metalloproteinase. It has been reported that 11 0 95 75 EJ -1 ECV-304 HT1080 OVISE MKN-45 HLE HSC-4 DLD -1 HEK293 11 0 95 75 EJ -1 ECV-304 HT1080 OVISE MKN-45 HLE HSC-4 DLD -1 HEK293 A B Fig. ... growth factor binding protein- related protein- 1 (IGFBP-rP1/angiomodulin/mac25) Sanjida Ahmed 1, 2 , Xinlian Jin 1 , Motoki Yagi 1, 2 , Chie Yasuda 1 , Yuichiro Sato 1, 2 , Shouichi Higashi 1 , Chen-Yong...
Ngày tải lên: 19/02/2014, 07:20
Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx
... separation 10 00 25+ 24+ 23+ 22+ 21+ 20+ 19 + 18 + 17 + 16 + 15 + 14 + 13 + 25+ 24+ 23+ 22+ 21+ 20+ 19 + 18 + 17 + 16 + 15 + 14 + 13 + 25+ 24+ 23+ 22+ 21+ 20+ 19 + 18 + 17 + 16 + 15 + 14 + 13 + 25+ 26+ 27+ 24+ 23+ 22+ 21+ 20+ 19 + 18 + 17 + 16 + 15 + 14 + 13 + 25+ 26+ 27+ 24+ 23+ 22+ 21+ 20+ 19 + 18 + 17 + 16 + 12 00 ... LLDNFHLMAP SEED 17 08.7 17 08.6 )0 .1 112 -12 1 +P NFHLMAP SEE 12 53.5 12 53.9 +0.4 11 3 -12 1 +P FHLMAP SEE 11 39.4 11 39.9 +0.5 16 1 -17 2 +P SLAKAQET SGEE 13 30.0 13 30.3 +0.3 Tryptic peptides 16 5 -17 5 +P AQET SGEEISK 12 58.5 ... 11 69.3 )0 .1 93 -10 3 +2P AG SPESPESTE 12 49.4 12 49.2 )0.2 93 -10 8 +P AGSPE SPESTEITEEE 17 70.7 17 71. 4 )0.3 10 0 -11 1 +P STEITEEELLD 13 57.6 13 57.6 0 10 9 -12 1 +P LLDNFHLMAP SEE 15 94.7 15 95.2 )0.5 10 9 -12 2...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
... ATP-hydrolyzing proteins, e.g. Escherichia coli DnaK (20 lm) [35], Hsc70 (1. 4 lm) [36], and F1- ATPase (15 lm) [37]. Furthermore, these proteins have V max values in the range 1. 1–3.5 nmolÆmin )1 Æmg )1 , comparable ... ± 1. 7 lM and V max ¼ 3.4 ± 0.3 nmolÆmin )1 Æmg )1 . Z. Popovic and D. M. Templeton ATP binding to IRP -1 FEBS Journal 274 (2007) 310 8– 311 9 ª 2007 The Authors Journal compilation ª 2007 FEBS 311 3 tional ... binding to IRP -1 FEBS Journal 274 (2007) 310 8– 311 9 ª 2007 The Authors Journal compilation ª 2007 FEBS 311 5 responsible for control of iron uptake. Arch Biochem Biophys 316 , 12 8 13 4. 6 Pantopoulos...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf
... this protein in metazoan cells. Experimental procedures Strains and media Strains W303-1B (mat a, ade2 1, ura3 1, his3 11 , trp1 1, leu2–3 ,11 2, can1 10 0), FYF4-A1 aco1 (mat a, ura3–52, trp1D63, ... Acad Sci 10 12, 1 13 . 3 Ogur M, Coker L & Ogur S (19 64) Glutamate auxo- trophs in Saccharomyces 1. I. The biochemical lesion in the glt -1 mutants-2. Biochem Biophys Res Commun 14 , 19 3 19 7. 4 ... iron-mediated redox regulation. J Biol Chem 275 , 16 227 16 234. 11 Kosman DJ (2003) Molecular mechanisms of iron uptake in fungi. Mol Microbiol 47, 11 85 11 97. 12 Bekri S, Kispal G, Lange H, Fitzsimons...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx
... EF0259 21, EF025922 and EF025923 (Received 20 November 2006, revised 15 January 2007, accepted 19 January 2007) doi :10 .11 11/ j .17 42-4658.2007.05709.x CCN4 ⁄ Wnt-induced secreted protein 1 (WISP1) is ... chondrocytes FEBS Journal 274 (2007) 16 55 16 65 ª 2007 The Authors Journal compilation ª 2007 FEBS 16 61 CCN4 ⁄ WISP1 and WISP1v proteins were actually translated from CCN4 ⁄ WISP1 and WISP1v mRNAs in HCS-2 ⁄ 8 ... chondrocytes Takeshi Yanagita 1, 2 , Satoshi Kubota 1 , Harumi Kawaki 1 , Kazumi Kawata 1 , Seiji Kondo 1 , Teruko Takano-Yamamoto 3 , Shinji Tanaka 4 and Masaharu Takigawa 1 1 Department of Biochemistry...
Ngày tải lên: 07/03/2014, 10:20
Báo cáo khoa học: Modulation of glucocorticoid receptor-interacting protein 1 (GRIP1) transactivation and co-activation activities through its C-terminal repression and self-association domains pptx
... described 1 1 013 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 10 13 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 14 62 1 1305 13 05 13 98 14 62 14 62 11 22 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 14 62 11 22 11 22 13 05 13 98 41 6 2 41 6 2 11 22 11 22 13 05 13 05 13 98 16 2 4 1 6 24 10 13 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 1 1 0 1 3 1 0 1 3 1 3 9 8 1 3 9 8 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 11 22 11 22 13 05 13 05 13 98 13 98 1 2 64 1 2 64 1 1 013 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 14 62 AD1 AD1 Repression ... described 1 1 013 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 10 13 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 14 62 1 1305 13 05 13 98 14 62 14 62 11 22 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 14 62 11 22 11 22 13 05 13 98 41 6 2 41 6 2 11 22 11 22 13 05 13 05 13 98 16 2 4 1 6 24 10 13 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 1 1 0 1 3 1 0 1 3 1 3 9 8 1 3 9 8 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 11 22 11 22 13 05 13 05 13 98 13 98 1 2 64 1 2 64 1 1 013 10 13 11 22 11 22 13 05 13 05 13 98 13 98 14 62 14 62 AD1 AD1 Repression ... domain AD2 AD2 AD3 AD3 13 05 13 05 13 05 13 05 13 98 14 62 14 62 11 22 11 22 + ? + 16 24 16 2 4 1 1 1 1 I II III III IV V 11 22 11 22 1 1 013 11 22 13 05 13 98 13 98 14 62 14 62 + ? 1 1 013 10 13 11 22 13 05 13 05 13 98 13 98 14 62 + Fig....
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx
... 2005, accepted 10 May 2005) doi :10 .11 11/ j .17 42-4658.2005.04759.x Death inducer obliterator protein 1 [DIDO1; also termed DIO -1 and death-associated transcription factor 1 (DATF -1) ] is encoded ... Journal 272 (2005) 3505–3 511 ª 2005 FEBS 3509 12 Shearn A (19 89) The ash -1, ash-2 and trithorax genes of Drosophila melanogaster are functionally related. Genetics 12 1, 517 –525. 13 Mazo AM, Huang DH, ... other functions [1] . Death inducer obliterator 1 [DIDO1; also termed DIO -1, death-associated transcription factor 1 (DATF- 1) ] is a protein described in humans and mice. DIDO1 was initially identified...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Plasmodium falciparum merozoite surface protein 1 Glycosylation and localization to low-density, detergent-resistant membranes in the parasitized erythrocyte pdf
... (387– 413 and 11 00 11 87) within the sequences of blocks 5 16 of the two dimorphic forms. The comparison could not be extended to the a-O-GlcNAc sites because the MSP -1 proteins of Thai-K1 and ... cytoplasmic glycoproteins. J. Biol. Chem. 262, 14 847 14 850. 15 . Hart, G.W. (19 97) Dynamic O-linked glycosylation of nuclear and cytoskeletal proteins. Ann.Rev.Biochem.66, 315 –335. 16 . Lyon, J.A., ... all MSP -1 epitopes [10 ]. Anti-CD59 mAb Correspondence to D. C. Hoessli, Department of Pathology, Centre me ´ dical universitaire, 1, rue Michel-Servet, 12 11 Geneva 4, Switzerland. Fax: + 41 22 7025746,...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Modified merozoite surface protein-1 peptides with short alpha helical regions are associated with inducing protection against malaria docx
... M6 (2 414 8, 24326), Q6 (23754) pale blue; L7 dark blue; Q8 pink; T9 (2 414 8, 24326), V9 (23754) orange; V10 grey (2 414 8, 23754 y 24324); M 11 (2 414 8, 24326), K 11 (23754) yellow; M12 (2 414 8), K12 (23754, 24326) ... A ˚ violations Maximum angular violations Immuno- genicity Protection 55 01 MLNISQHQCVKKQCPQNS –– 2 414 8 MLNISMLQTVMMMTPQK 16 2 0 .19 0.30 2 + + 23754 MHNISQLQVVKKMVPQK 14 1 0.45 0.40 3 + – 24326 MLNISMLQTVMKMTPQK 15 1 0. 51 0.30 2 – – Fig. 3. Families ... against malaria Mary H. Torres 1 *, Luz M. Salazar 1 *, Magnolia Vanegas 1 , Fanny Guzman 1 , Raul Rodriguez 1 , Yolanda Silva 1 , Jaiver Rosas 1 and Manuel E. Patarroyo 1, 2 1 Fundacion Instituto de...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: CXXC finger protein 1 restricts the Setd1A histone H3K4 methyltransferase complex to euchromatin doc
... 2009, accepted 4 November 2009) doi :10 .11 11/ j .17 42-4658.2009.07475.x CXXC finger protein 1 (Cfp1), encoded by the CXXC1 gene, is a compo- nent of the euchromatic Setd1A histone H3K4 methyltransferase ... 417 25– 417 31. 16 Lee JH, Tate CM, You JY & Skalnik DG (2007) Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex. J Biol Chem 282, 13 419 13 428. 17 ... Setd1 compo- nent, the subunit composition of the mammalian Setd1A and Setd1B HMTase complexes are identical [16 ], each containing CXXC finger protein 1 (Cfp1), Rbbp5, Wdr5, Ash2, and Wdr82 [15 ,16 ]....
Ngày tải lên: 15/03/2014, 09:20
Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Mixed lineage kinase LZK and antioxidant protein-1 activate NF-jB synergistically docx
Ngày tải lên: 17/03/2014, 09:20
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