Genetic and molecular dissection of DELLA related GA signaling pathway in regulating arabidopsis seed germination

... 1.2 DELLA genes encode a group of negative regulators in GA- signaling Pathway 1.3 The important role of RGL2 in regulating seed germination 1.4 Regulation of seed germination by both internal and ... RGA and GAI to repress seed germination in the dark 84 4.2.4 Combination of loss -of- function of GAI, RGA, RGL1 and RGL2 leads to both light- and G...

Ngày tải lên: 13/09/2015, 20:31

... ! ! MULTI& STRATEGIES, FOR, CONTROL, OF, MOTILITY, VIA, MORA, SIGNALING, PATHWAY, IN, PSEUDOMONAS, PUTIDA, , , , , , , , , NG WEI LING (B Sc.(Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... messenger signaling Genomic and signaling studies on new models led to the finding that signaling proteins are typically modular in nature with each con...

Ngày tải lên: 09/09/2015, 17:53

... postinfection, we observed a marked difference between the number of CFUs of the wild-type strain and that of the PMM56 mutant strain in lungs and in the spleen (Fig 7A) Indeed, both strains ... cell envelope of M tuberculosis and to virulence [3–6] Nevertheless, their precise molecular mechanisms of action are still unknown, and the specific roles of...

Ngày tải lên: 23/03/2014, 09:20

... A novel high-activity D-hydantoinase from Jannaschia sp CCS1 obtain optically pure amino acids, namely chemical and enzymatic syntheses Chemical synthesis gives racemic mixtures of amino acids ... precipitate fraction; sup, supernatant fraction The molecular weight standard (lane M) is indicated on the right A novel high-activity D-hydantoinase from Jannaschia sp...

Ngày tải lên: 18/02/2014, 08:20

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE As shown in Fig 4, 9-HODE is the main product of the hazelnut ... stages of seed development, and which is the only LOX detected in pea leaves [25] Considering the high level of expression of CaLOX2 in hazelnut seeds, it would be of...

Ngày tải lên: 21/02/2014, 00:20

... hp0547 Cagf ⁄ Cag1 Cage ⁄ Cag2 Cagd ⁄ Cag3 Cagc ⁄ Cag4 Cagb ⁄ Cag5 Caga ⁄ VirB11 CagZ CagY CagX CagW CagV CagU CagT CagS CagQ CagP CagM CagN CagL CagI CagH CagG CagF CagE CagD CagC CagB CagA 116 ... both conserved and unique components of the Cag- T4SS, together with potential implications for the current understanding of its mode of action The cagPAI and the Cag...

Ngày tải lên: 06/03/2014, 00:21

... although, as V volvacea produces at least one other laccase isoform in addition to lac1 [1], this and possibly other laccase isoforms may be contributing to the total laccase activity detected at the ... primers The putative N-glycosylation site is boxed; *; stop codon The putative polyadenylation signals (TATAAA and CATAAA) are in white on a black background Ó FEBS 200...

Ngày tải lên: 07/03/2014, 15:20

... bonds stabilizes a conformational epitope of the apical membrane antigen-1 of Plasmodium falciparum [27] Although the sequential epitope of VP1 of foot and mouth disease does not contain intramolecular ... induced against the linear isoform of the HNE peptide Although the binding pattern may be somewhat blurred by these antibodies and by the polyclonal nature...

Ngày tải lên: 08/03/2014, 08:20

... blue) and standard (magenta) substrates after ns of MD (C) Overlay of positions of N-propionyl modified (light blue) and standard (magenta) substrates after ns of MD (D) Molecular surface of the ... carbohydrates (left panel), protein (middle panel) and for enzymatic activity (right panel) Lane 1, PoHex; lane 2, PoHex plus Endo H; lane 3, PoHex plus a-mannosidase; lane 4, E...

Ngày tải lên: 14/03/2014, 23:20

... proteomics and variations of Bf venom, we studied individual venom of two specimens of Bf from Kolkata, India (designated as KBf) by a comparative proteomic and genomic approach The venom PLA and 3FTx ... structure of an inactive mutant of phospholipase A2 in the venom of Bungarus fasciatus (banded krait) J Biochem (Tokyo) 112, 707–713 Venom proteins of...

Ngày tải lên: 16/03/2014, 12:20

... case of arthropods [14–17] Molecular cloning of ST94 cDNA The cDNA cloning of tyrosinase ST94 was carried out by degenerate RT-PCR and RACE using the first strand cDNA of poly(A)+ RNA extracted from ... trypsin The molecular masses of the subunits of ST94 and ST94t were similar to those of the proenzymes ( 70–80 kDa) and the activated enzymes by pro...

Ngày tải lên: 17/03/2014, 10:20

... catalyze the same deamidating reaction on related Molecular action of Pasteurella multocida toxin G-protein targets and with overlapping cellular outcomes, the sequence and structure of the activity ... current understanding of the structure–function, mechanism of action and cellular consequences of this newest member of the G -protein-deamidating...

Ngày tải lên: 22/03/2014, 15:21

... was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant cellobiohydrolase (A) SDS/ PAGE [10% (w/v) gel] analysis of ... (1985) Isolation and characterization of the endoglucanases of Talaromyces emersonii Biochem J 225, 365–374 29 McHale, A & Coughlan, M (1988) Purification of beta...

Ngày tải lên: 23/03/2014, 13:20

... alignment of the N-terminal sequence of ChPLA2 with those of TPLA2 [14], OPLA2 [15], DrPLA2 [13], PPLA2 [20], bovine PLA2 [20], and human PLA2 [21] The N-terminal sequences of bird PLA2s exhibit ... TPLA2, OPLA2 DrPLA2, bovine PLA2 and human PLA2 Identical amino acids are in bold, and homologous amino acids are in italic ChPLA2 PPLA2 TPLA2 OPLA2 DrPLA2 Bovine PLA2 Human PLA2 :AL...

Ngày tải lên: 30/03/2014, 01:20