Genetic and molecular dissection of DELLA related GA signaling pathway in regulating arabidopsis seed germination

... 1.2 DELLA genes encode a group of negative regulators in GA- signaling Pathway 1.3 The important role of RGL2 in regulating seed germination 1.4 Regulation of seed germination by both internal and ... RGA and GAI to repress seed germination in the dark 84 4.2.4 Combination of loss -of- function of GAI, RGA, RGL1 and RGL2 leads to both light- and GA- independent seed germination 90 4.2.5 Combination ... To investigate whether DELLA genes are involved in the flowing of the light signal to GA and to seed germination and to evaluate the roles of each DELLA protein in regulation of seed germination, ...

Multi strategies for control of motility via mora signaling pathway in pseudomonas putida

... ! ! MULTI& STRATEGIES, FOR, CONTROL, OF, MOTILITY, VIA, MORA, SIGNALING, PATHWAY, IN, PSEUDOMONAS, PUTIDA, , , , , , , , , NG WEI LING (B Sc.(Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... messenger signaling Genomic and signaling studies on new models led to the finding that signaling proteins are typically modular in nature with each conserved domain performing a distinct biochemical ... knockout strains of various genes of interest namely: morC, cyaA and opuAC were also created for further studies To understand the role of CyaA and OpuAC in MorA signaling pathway controlling motility...

Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc

... postinfection, we observed a marked difference between the number of CFUs of the wild-type strain and that of the PMM56 mutant strain in lungs and in the spleen (Fig 7A) Indeed, both strains ... cell envelope of M tuberculosis and to virulence [3–6] Nevertheless, their precise molecular mechanisms of action are still unknown, and the speciﬁc roles of the various members of the DIM family, ... DIM A and DIM B, in these functions have never been investigated The recent developments in our understanding of the biosynthetic pathway of DIMs have provided the means to investigate the contribution...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... A novel high-activity D-hydantoinase from Jannaschia sp CCS1 obtain optically pure amino acids, namely chemical and enzymatic syntheses Chemical synthesis gives racemic mixtures of amino acids ... precipitate fraction; sup, supernatant fraction The molecular weight standard (lane M) is indicated on the right A novel high-activity D-hydantoinase from Jannaschia sp CCS1 Fig Puriﬁcation of HYDBp ... high-activity D-hydantoinase from Jannaschia sp CCS1 Y Cai et al Experimental procedures Genome mining and identiﬁcation of putative D-hydantoinase genes Using the amino acid sequence of HYDBp (AAL37185)...

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE As shown in Fig 4, 9-HODE is the main product of the hazelnut ... stages of seed development, and which is the only LOX detected in pea leaves [25] Considering the high level of expression of CaLOX2 in hazelnut seeds, it would be of interest to isolate and characterize ... characterize hazelnut lipoxygenases at the biochemical and molecular level Materials and methods Plant material Hazelnut (Corylus avellana L cv Mortarella) leaves and seeds were harvested at different...

Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc

... hp0547 Cagf ⁄ Cag1 Cage ⁄ Cag2 Cagd ⁄ Cag3 Cagc ⁄ Cag4 Cagb ⁄ Cag5 Caga ⁄ VirB11 CagZ CagY CagX CagW CagV CagU CagT CagS CagQ CagP CagM CagN CagL CagI CagH CagG CagF CagE CagD CagC CagB CagA 116 ... both conserved and unique components of the Cag- T4SS, together with potential implications for the current understanding of its mode of action The cagPAI and the Cag type IV secretion apparatus Gene ... complex proteins A further step in the assembly of the secretion apparatus would be the recruitment of components forming the inner membrane pore of the secretion apparatus The Cag system contains...

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... although, as V volvacea produces at least one other laccase isoform in addition to lac1 [1], this and possibly other laccase isoforms may be contributing to the total laccase activity detected at the ... primers The putative N-glycosylation site is boxed; *; stop codon The putative polyadenylation signals (TATAAA and CATAAA) are in white on a black background Ó FEBS 2003 Laccase gene from Volvariella ... min; then a ﬁnal extension at 72 °C for 10 The primers for lac1 PLAC1F (5¢-AGCTTT CATTCCCAGTGATTG-3¢) and PLAC1R (5¢-AACGAG CTCAAGTACAAATGACT-3¢) were designed according to our cloned cDNA (GenBank...

Báo cáo khoa học: Functional ﬁne-mapping and molecular modeling of a conserved loop epitope of the measles virus hemagglutinin protein pdf

... bonds stabilizes a conformational epitope of the apical membrane antigen-1 of Plasmodium falciparum [27] Although the sequential epitope of VP1 of foot and mouth disease does not contain intramolecular ... induced against the linear isoform of the HNE peptide Although the binding pattern may be somewhat blurred by these antibodies and by the polyclonal nature of the sera, the importance of residues ... information is available and data about the role of the cysteines are conﬂicting Hu & Norrby [24] suggested that C381 and C494 participate in unspeciﬁed intramolecular Ó FEBS 2003 Fine-mapping of an...

Báo cáo khoa học: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosaminidase pot

... blue) and standard (magenta) substrates after ns of MD (C) Overlay of positions of N-propionyl modiﬁed (light blue) and standard (magenta) substrates after ns of MD (D) Molecular surface of the ... carbohydrates (left panel), protein (middle panel) and for enzymatic activity (right panel) Lane 1, PoHex; lane 2, PoHex plus Endo H; lane 3, PoHex plus a-mannosidase; lane 4, Endo H; lane 5, a-mannosidase ... buffer system of Laemmli [21] (Fig 1C, lanes and 9, respectively) Another protein band with a 2470 Fig Optimization of PoHex production and puriﬁcation (A), (B) Time course of secretion of PoHex from...

Báo cáo khoa học: Sequences, geographic variations and molecular phylogeny of venom phospholipases and threeﬁnger toxins of eastern India Bungarus fasciatus and kinetic analyses of its Pro31 phospholipases A2 docx

... proteomics and variations of Bf venom, we studied individual venom of two specimens of Bf from Kolkata, India (designated as KBf) by a comparative proteomic and genomic approach The venom PLA and 3FTx ... structure of an inactive mutant of phospholipase A2 in the venom of Bungarus fasciatus (banded krait) J Biochem (Tokyo) 112, 707–713 Venom proteins of Bungarus fasciatus Liu CS, Chang CS, Leu ... high Ca2+ binding afﬁnity Finally, phylogenetic analyses of the elapid PLAs and the krait 3FTx were carried out to Venom proteins of Bungarus fasciatus better understand the intrageneric and intergeneric...

Báo cáo khoa học: Puriﬁcation, characterization and molecular cloning of tyrosinase from the cephalopod mollusk, Illex argentinus docx

... case of arthropods [14–17] Molecular cloning of ST94 cDNA The cDNA cloning of tyrosinase ST94 was carried out by degenerate RT-PCR and RACE using the ﬁrst strand cDNA of poly(A)+ RNA extracted from ... trypsin The molecular masses of the subunits of ST94 and ST94t were similar to those of the proenzymes ( 70–80 kDa) and the activated enzymes by proteolysis ( 60–70 kDa) of a bivalve tyrosinase ... activated tyrosinase, one of the abundant proteins in the ink In this paper, we describe the puriﬁcation, proteolytic activation, some enzymatic properties and molecular cloning of the squid tyrosinase...

Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx

... catalyze the same deamidating reaction on related Molecular action of Pasteurella multocida toxin G-protein targets and with overlapping cellular outcomes, the sequence and structure of the activity ... current understanding of the structure–function, mechanism of action and cellular consequences of this newest member of the G -protein-deamidating dermonecrotic toxin family Epizootic and zoonotic ... Wilson and M Ho Molecular action of Pasteurella multocida toxin One enigmatic aspect of PMT action on its G-protein substrates is the ability of PMT to discriminate among the different Ga isoforms,...

Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the ﬁlamentous fungus, Talaromyces emersonii ppt

... was 0.7 and overall G-factor, a measure of the normality of the structure, was 0.0 Fig SDS/PAGE and activity analysis of recombinant cellobiohydrolase (A) SDS/ PAGE [10% (w/v) gel] analysis of ... (1985) Isolation and characterization of the endoglucanases of Talaromyces emersonii Biochem J 225, 365–374 29 McHale, A & Coughlan, M (1988) Puriﬁcation of beta glucosidases from Talaromyces emersonii ... classiﬁcation of cellulases Eur J Biochem 258, 200–206 68 Takashima, S., Iikura, H., Nakamura, A. , Hidaka, M., Masaki, H & Uozumi, T (1998) Isolation of the gene and characterization of the enzymatic...

Báo cáo khoa học: Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A2 docx

... alignment of the N-terminal sequence of ChPLA2 with those of TPLA2 [14], OPLA2 [15], DrPLA2 [13], PPLA2 [20], bovine PLA2 [20], and human PLA2 [21] The N-terminal sequences of bird PLA2s exhibit ... TPLA2, OPLA2 DrPLA2, bovine PLA2 and human PLA2 Identical amino acids are in bold, and homologous amino acids are in italic ChPLA2 PPLA2 TPLA2 OPLA2 DrPLA2 Bovine PLA2 Human PLA2 :ALWEF :ALWQF ... that of known mammal PLA2s Molecular modeling was also proposed to explain some biochemical differences between ChPLA2 and other pancreatic PLA2 Results and discussion Puriﬁcation of ChPLA2 Thirty...

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