Bio nanoparticles and bio microfibers for improved gene transfer into glioma cells
... BIO- NANOPARTICLES AND BIO- MICROFIBERS FOR IMPROVED GENE TRANSFER INTO GLIOMA CELLS YANG JINGYE (B Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS GRADUATE SCHOOL FOR INTEGRATIVE ... procedures to form bio- nanoparticles and bio- microfibers The findings in this study should enrich the development of gene therapies for CNS diseases, p...
Ngày tải lên: 12/09/2015, 09:10
... overview of the progress made by using different types of promoters and vectors for gene delivery to the CNS 1.1 Current progress in gene therapy Gene therapy, which usually refers to somatic gene therapy, ... by the selection of promoters The gene delivery vector that carries and transfers the gene into the nuclei of target cell is another...
Ngày tải lên: 16/09/2015, 08:30
... functions in different ways 5. 2 Future prospective Agrobacterium was demonstrated to be able to deliver its DNA into mammalian cells Nonetheless, the precise mechanism of gene transfer is still unclear ... occurrence of horizontal gene transfer among prokaryotes is well established, the gene transfer between bacteria and eukaryotic cells is less well elucidated The...
Ngày tải lên: 17/09/2015, 17:20
Molecular basis of agrobacterium mediated gene transfer into mammalian cells 4
... _ _ + + + + pH chvG 6 6) 7) G G 14 P3 A A C pL 40 07 pT 6( 6( 63 60 G G A A A 0-A 34 40 63 A A Fig 4. 4 The effects of ChvG on the expression of acid-inducible genes aopB and katA as determined ... bacterial cells were analyzed in the absence (-) [A6 340 , A6 340 -CG9, A6 340 (pJYH15) and A6 340 -AG6] or presence (+) [A6007-CG9, A6007(pJYH15), A6007-AG6, A6 340 -AG6(pLP36) and A6 34...
Ngày tải lên: 17/09/2015, 17:20
Molecular basis of agrobacterium mediated gene transfer into mammalian cells 3
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Ngày tải lên: 17/09/2015, 17:20
Molecular basis of agrobacterium mediated gene transfer into mammalian cells 2
... Dissolved in dH2O, filter sterilized 24 24 24 X-Gal Dissolved in dimethyl sulfoxide 20 20 20 Proteinase K Dissolved in dH2O 20 50 50 RNase Dissolved in dH2O 10 20 20 51 Table 2. 4 Mammalian and ... study of the staining pattern of rhodamine phalloidin, mammalian cells were seeded on the glass cover-slip in 6-well tissue culture plates 2. 8.3 Agrobacterium- mediated ge...
Ngày tải lên: 17/09/2015, 17:20
Molecular basis of agrobacterium mediated gene transfer into mammalian cells 1
... T-DNA transfer will be described in detail 1. 1 .1 Roles of Ti-plasmid encoded virulence genes 1. 1 .1. 1 VirA/VirG, member of highly conserved class of two-component regulatory system Sensing of signal ... some mammalian cells 1. 2 .1 T-DNA transfer from A tumefaciens into yeast T-DNA transfer from A tumefaciens into S cerevisiae is very similar to T-DNA transfer...
Ngày tải lên: 17/09/2015, 17:20
Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system
... GENETIC ENGINEERING AND SURFACE MODIFICATION OF BACULOVIRUS DERIVED VECTORS FOR IMPROVED GENE DELIVERY TO THE CENTRAL NERVOUS SYSTEM YANG YI (B Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR ... Chapter Three: Genetic Engineering of Baculovirus Vectors for Controlled Gene Delivery ………65 3.1 Introduction………………………………………………………………… 66 3....
Ngày tải lên: 14/09/2015, 11:28
Tài liệu ADC PROFESSIONAL SERVICES ADC Decommissioning and Removal Services for Improved Network Performance and Utilization docx
... cable racking and infrastructure DECOMMISSIONING AND REMOVAL SERVICES ADC decommissioning and removal services can help operators reduce both operation and capital expenditures, and improve their ... equipment and training Network/ environment compliance To help operators reclaim space and optimize their network assets and facilities, ADC offers turnkey ser...
Ngày tải lên: 09/12/2013, 17:15
nanotechnology and nanomaterials promises for improved tissue regeneration
... devices and polymers used as nerve conduits) Nanotechnology and nanomaterials: Promises for improved tissue regeneration used for neural tissue repair have been limited by the extensive formation ... cartilage repair Because the Nanotechnology and nanomaterials: Promises for improved tissue regeneration 71 Figure Self-assembled nanofibers and nanotubes fo...
Ngày tải lên: 20/03/2014, 13:05
Báo cáo hóa học: " Research Article Binocular Image Sequence Analysis: Integration of Stereo Disparity and Optic Flow for Improved Obstacle Detection and Tracking" pptx
... calculate the optic flows for the left and right image sequences 2.1 Optic flows of moving obstacles Many approaches have been designed for obtaining optic flows for moving objects from image sequences ... “Cooperative integration of stereopsis and optical flow computation,” Journal of the Optical Society of America A, vol 12, no 12, pp 2564–2572, 1995 [14] S P Cliffo...
Ngày tải lên: 21/06/2014, 22:20
Báo cáo hóa học: " Pd Nanoparticles and Thin Films for Room Temperature Hydrogen Sensor" potx
... was used to study structure and morphology of the films Hydrogen Sensor Characterization Hydrogen sensing performance of Pd thin film (via sputtering deposition) and Pd nanoparticles (via electrochemical ... function on hydrogen exposure is attributed to electronic sensitization Conclusions Electrochemically grown Pd nanoparticles and sputtered Pd thin films have been...
Ngày tải lên: 22/06/2014, 00:20
Báo cáo y học: " Human herpesvirus 6 major immediate early promoter has strong activity in T cells and is useful for heterologous gene expression" ppsx
... 6MIEpF-381 5’-agt cgg tac cac att cct gtt tca tga tgt gta gc-3’ 6MIEpF-214 5’-agt cgg tac ctc ctg ttt ttg agt aag ata tga c-3’ 6MIEpF- 165 5’-agt cgg tac cag cta att tcc att cca tat ttg tc-3’ 6MIEpF-102 ... OCT-1-binding site (Figure 3) The transcriptional regulators that bind to these sites might enhance the promoter activity of HHV -6 MIEp Interestingly, the promoter construct that c...
Ngày tải lên: 11/08/2014, 21:21
Báo cáo y học: "Drug monitoring in child and adolescent psychiatry for improved efficacy and safety of psychopharmacotherapy" pdf
... Description of the multi-centre drug monitoring data base Because for many of the psychotropic drugs applied in the Child and Adolescent and Psychiatry data on pharmacokinetics, efficacy and side ... drugs in children and adolescents, is described Safety of psychotropic medications in children: A developmental issue Current situation in the psychopharmacolo...
Ngày tải lên: 13/08/2014, 18:21