methods for muscle gene transfer in large animal models

... up to minimise the increase in the average coefficient of inbreeding of the resulting progeny The average coefficients of inbreeding within line and generation were computed from the inverse ... weight in a series comprising five or six individuals from each line Animals slaughtered in a given were from the same fattening batch The animals fasted for 16 h before they were transported for ... nested within generation, generation, line and line x generation combination Annual selection differentials were calculated in each line, from differences between the average performance of animals...

Ngày tải lên: 09/08/2014, 18:21

... average, in the S line, and the genetic trend was larger in the gluteus superficialis than in the biceps femoris, i.e in the muscle showing the lowest average water-holding capacity and the largest ... covariances in sparse linear models, Genet Sel Evol 30 (1998) 3-26 [26] Przybylski W., Vernin P., Monin G., Relationship between glycolytic potential and ultimate pH in bovine, porcine and ovine muscles, ... semispinaLis capitis muscle was unreliable owing to the low heritability value of the latter trait In the S line, the increase in ultimate pH was small in all ’white’ and ’intermediate’ muscles,...

Ngày tải lên: 09/08/2014, 18:21

... selected line In fact, the genetic trend in average daily gain shows a slight increase in the selected line compared with the control line Detailed examination of the genetic trend in this trait in ... per line for each generation When averaging breeding values for a trait, only individuals having a record for that trait were taken into account The genetic trend was estimated by the linear regression ... but negative for backfat depths In response to downward selection on IVGP, average daily gain sharply increased in the selected line in the first two generations Afterwards, the genetic difference...

Ngày tải lên: 09/08/2014, 18:21

... dexamethasone in a 5% CO2 incubator All other cells were maintained at 37°C in DMEM containing mM glutamine, 10% Fetal Calf Serum (FCS), 100 U/mL penicillin and 100 µg/mL streptomycin, similarly in a ... number not for citation purposes) Genetic Vaccines and Therapy 2004, 2:6 incubator 293, 293T and NIH3T3 were maintained in DMEM containing 10% FCS, mM glutamine, 100 U/ml penicillin and 100 µg/ml ... positive for gene transfer Transduction – studies of vector decay Cell-free viral vector-containing supernatant was incubated at 37°C for 30 min, hr, hr, or 24 hr prior to being used as the transducing...

Ngày tải lên: 14/08/2014, 19:22

... entailment information when identifying answers to questions Results from this baseline are presented in Table Creating New Sources of Training Data In order to obtain more training data for our ... System Processing textual entailment, or recognizing whether the information expressed in a text can be inferred from the information expressed in another text, can be performed in four ways We ... to the original question Integrating Textual Entailment in Question Answering In this section, we describe three different methods for integrating a textual entailment (TE) system into the architecture...

Ngày tải lên: 08/03/2014, 02:21

... patterning and informational ordering in the sentence This is exemplified by the constructions of sentences (2) - (5) above While expressing the same propositional content, the order of information ... constructions offers interesting insights into the constructional inventory of a language It also opens up the possibility of comparing seemingly closely related languages in terms of the sets ... can be used in further exploratory work Alternatively, a file with all assessed candidates can be generated to serve as training data for identification methods based on machine learning Conclusions...

Ngày tải lên: 16/03/2014, 20:20

... extensively investigated by other researchers including foreign ones Additional information in such problems is provided in the integral form and admits a physical interpretation as a result of measuring ... merely for linear and semilinear equations In this book the solvability of the preceding problem is revealed for a more general class of quasilinear equations The approximate methods for constructing ... noting that the characteristics of the boundary being an equilibria in thermodynamics lead to supplementary problems for investigating inverse problems with the final overdetermination, since in...

Ngày tải lên: 17/03/2014, 14:30

... Morquio gene transfer (SUMF1) gene has permitted a two-fold to three-fold increase in the corresponding sulfatase enzyme activity SUMF1 encodes the enzyme converting serine to formylglycine at ... studies in animal models of lysosomal storage diseases (LSDs) have shown that after a single vector administration, therapeutic enzyme levels can be maintained with clinical benefits for up to ... disease for gene therapy, owing to the lack of central nervous system involvement [2] To date, no in vivo gene therapy trial has been performed for MPS IVA; one report demonstrated, using a retroviral...

Ngày tải lên: 29/03/2014, 21:20

... pixels, which affects in reducing phantom colors in the resulting image In our approach, we calculate filter weights using information from color frame in RGB, while applying filtering on depth map ... neighborhoods are formed for every pixel in the image domain X Once adaptive neighborhoods are found, one must find some modeling for depth channel before utilizing this structural information Constant ... modeling is simple, it has proven quite effective for mitigating the blocking artifacts arising from quantization of transform coefficients [14] In particular, it allows for establishing a direct link...

Ngày tải lên: 21/06/2014, 17:20

... (eV) (a) 18 19 20 21 Kinetic energy (eV) Binding energy (eV) 22 -1 (b) Figure UPS spectra in the low kinetic and low binding energy regions (a) UPS spectra in the low kinetic energy region from ... energy hν By subtracting the binding energy of the low energy cutoff from the high binding energy edge of the UPS spectra, the work function of the sample is obtained [8] The change in the work function ... wt.%) Thin films of PEDOT:PSS and P3HT + SMU Page of PCBM were formed on the ITO-coated glass by spin-coating The spin speed of the polymer film was 4,000 rpm for PEDOT:PSS and 1,000 rpm for P3HT...

Ngày tải lên: 21/06/2014, 17:20

... allowed for errors in trypsin specificity Carbamidomethylation of cysteines was taken as fixed modifications, and methionine oxidation and formylation of lysine, arginine and N-terminal amino acids ... supporting the proteomic results Notably, nine cases of LCNEC including four used for the proteomic experiments Nomura et al Journal of Clinical Bioinformatics 2011, 1:23 http://www.jclinbioinformatics.com/content/1/1/23 ... the data containing small spectral counts including zero Statisticians, however, showed that the results of G-test using a contingency table containing small counts are not so convincing because...

Ngày tải lên: 10/08/2014, 09:22

... reporter gene to delineate the optimal period for subsequent HSV-tk gene transfer in synchronized cells, we focused our investigation for the transfer of the suicide gene HSV-tk in a time window for ... effective in synchronizing DHDK12 cells in S phase than ara-C or aphidicolin Consequently, the efficacy of MTX in synchronizing cells in S phase was then tested in the HT29 cell line In HT29 cell line, ... (Labtek II-Nunc), were fixed for 15 at 4°C with 4% paraformaldehyde and incubated with PBS containing 0.2% serum bovine albumin (SAB) and 0.1% saponin for Cells were incubated with anti-HSV-TK...

Ngày tải lên: 10/08/2014, 10:21

... is also influenced by surface tension, smooth muscle contraction and peripheral airway inhomogeneity Numerous methods for determining RL and Cdyn have been described in anesthetized and instrumented ... (FRC) in conscious mice is normally maintained with active inspiratory muscle tone that is minimal or eliminated in the anesthetized animal [16] Lung volume changes must be assessed by calibrating ... applying them in the mouse Unfortunately, at the present time, there is no gold standard for measuring lung function in mice, since none of the available methods is optimal in all regards Some investiga-...

Ngày tải lên: 12/08/2014, 15:21

... was followed by the reinforcing mats placing team These were transported down the face with the use of a trolley travelling on two rail lines The reinforcing mats were placed in the middle of the ... per cubic metre Reinforcing of 0.4% was adopted in each direction in the center of the slab The slabs were concreted using a 15m wide slip form The sealing of the perimeter joint consists of: ... has been published in international Engineering magazines on Dam construction, instrumentation and embankment behaviour He has taken part in international symposiums and meetings and presented...

Ngày tải lên: 13/08/2014, 22:21

... pre-alveolate in origin As more genome data become available and flanking genes can be examined for each gene in a larger context, positional information will be informative in distinguishing among ... genome-wide search for intracellular and horizontal gene transfers in C parvum was carried out We systematically determined the evolutionary origins of genes in the genome using phylogenetic approaches, ... Glimmer-predicted protein-coding regions of the C parvum genome (5,519 sequences) were used as input for phylogenetic analyses using the PyPhy program [27] In this program, phylogenetic trees for each input sequence...

Ngày tải lên: 14/08/2014, 14:21

... were injected into the crushed sciatic nerve of rats Animals were euthanized after weeks An Xgal staining kit was used to detect cells expressing the transgene Eosin was used for counter-staining ... transduction using an X-Gal staining kit (Invitrogen) In order to identify neurons and glia in the cultures transduced with the EGFP-encoding vectors, ICC was performed with antibody directed against microtubule ... For example, vectors encoding β-Gal have been titrated using X-Gal staining [28] Also significant for titration is the cell line used as receptors for a given pseudotype may vary from cell line...

Ngày tải lên: 14/08/2014, 19:22

... methods for determining structural properties and conditions Information derived from conducting these types of tests is important in evaluating the integrity of a concrete structure Such information ... pile shaft is indicated by change in slope of line representing arrival time of stress pulse as function of depth) radiometric methods and radiographic methods Both involve gaining information about ... additional information in Carino (1992) Fig 2.5.2 is a schematic of a continuous eddy-current covermeter In the absence of a reinforcing bar, the magnitude of the alternating current in the coil...

Ngày tải lên: 24/10/2014, 17:44

... connection and routing within the surrounding area 1.2 Port Container Terminals and Quayside Operations In general terms, the container handling activities in a typical port container terminal can be ... container terminal Quay Crane Quayside Operation Yard Crane HIT Truck Vessel Discharging container flow Loading container flow Figure 1.2: Schema for container ow in a port container terminal ... indented berths for mega-containerships At rst, the authors rewrote the formulation of the problem proposed in Nishimura et al (2001), modifying the original non-linear integer programming formulation...

Ngày tải lên: 10/09/2015, 15:48

... domain is in nite in ζ To deal with the in nite domain we should either transfer the boundary conditions from in nity or introduce the basis functions for a spectral method defined in the in nite ... algebraically decaying boundary layer near the apex of a cone, there has been no direct evidence for the algebraically decaying boundary layers in inflowing flows in cones To summarize, in owing flows in cones ... derived from the original differential equation In Galerkin methods, the original equation is multiplied by the basis functions and integrated over the domain In the Petrov-Galerkin generalization,...

Ngày tải lên: 11/09/2015, 09:05

... proteins 40 3.1 Introduction 41 3.2 General strategy for side-chain assignments of methyl-containing residues 44 3.2.1 Methyl assignments 44 3.2.2 Assignment of side-chain protons in methyl-containing ... strategy for structure determination of large proteins in solution without deuteration 4.1 Introduction 56 57 iv    Development of NMR methods for the structural elucidation of large proteins Table ... presented in chapter for assigning protons of methyl-containing residues of uniformly 13 C- labeled large proteins Secondary, I present a novel strategy in chapter to assign backbone and side chain...

Ngày tải lên: 11/09/2015, 09:57