Roles of rho small GTPase in zebrafish development

... proteins of RhoA have been discovered, including protein kinases (protein kinase N/protein kinase C-related kinase (PKN/ PRK1), PRK2, citron kinase), non-kinases (Rhophilin, Rhotekin, Kinectin), ... profilin, an actin monomer-binding protein, through their FH domain [Sohn et al 1994] This interaction allows them to bind to the barbed ends of actin filaments, which antagonizes the binding...

Ngày tải lên: 12/09/2015, 08:19

... coronal section and scheme of the coronal section at E14.5 in (F) (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline Surprisingly, the ... binding is reduced, indicating that HSF4 facilitates HSF1 binding via chromatin remodelling Heat shock genes are not HSF4 targets, but HSF4 regulates a set of nonclassical h...

Ngày tải lên: 18/02/2014, 04:20

... probable role of auxin-responsive genes in reproductive development and abiotic stress signaling in rice Transcript profiling of auxin-responsive genes study from our laboratory, the rice coleoptile ... 2009 FEBS M Jain and J P Khurana Transcript profiling of auxin-responsive genes Fig Expression profiles of auxin-responsive genes in various...

Ngày tải lên: 07/03/2014, 01:20

... def (digestive- organ expansion factor) IS A CRUCIAL GENE FOR THE DEVELOPMENT OF ENDODERM- DERIVED ORGANS IN ZEBRAFISH (Danio rerio) RUAN HUA (M.Sc., Wuhan University, P.R.China) A THESIS SUBMITTED ... before reviewing zebrafish intestine, liver and pancreas organogenesis 1.3.1 Endoderm formation in zebrafish 1.3.1.1 Endoderm formation and...

Ngày tải lên: 11/09/2015, 16:06

... paracrine signaling in liver development The coexpression of hgfa and c- met in pectoral fin, hgfb and c- met in proneprhic duct also indicate their paracrine signaling in the development of these ... Characterizing HGF and its receptor c- met s role in zebrafish development (Manuscript in preparation) v LIST OF FIGURES Fig.1.1 Schematic represent...

Ngày tải lên: 12/09/2015, 08:18

... 1.1.3.1 Pin1 function in M phase 1.1.3.2 Pin1 function in G1 and S phase 1.1.3.3 Pin1 function in oncogenesis .9 1.1.4 Pin1 function in apoptosis 10 1.1.5 Pin1 function in ... reduction of zPin1 expression in zPin1 morphant embryos 79 Figure 3.8 Developmental delay in zPin1 morphant embryos 82 Figure 3.9 Neurogenin1 staining of the wild type embryos and c...

Ngày tải lên: 12/09/2015, 09:55

... the hindbrain and its deficiency in morphants is in line with expression of these genes in this part of brain ctrl/brain A gli2b MO/brain B ctrl/trunk D gli2 MO/brain C gli2b MO/trunk E gli2 ... that in Gli2b morphant (data now shown) These results indicated a minor role of both gli2 and gli2b in regulating expression of nkx2.2 in the ventral hindbrain Due to d...

Ngày tải lên: 15/09/2015, 17:10

... containing gli2 genes 101 Results 3 .2 Expression analysis of gli2b in zebrafish 3 .2. 1 Temporal expression of gli2b in zebrafish embryos Temporal expression of gli2b was first compared with that of ... hpf gli2b dorsal smu-/-/48 hpf B gli2b dorsal syu-/-/48 hpf C gli2b dorsal cyclopamine/48 hpf E gli2b WT/48 hpf D gli2b dorsal shh mRNA/48 hpf F Fig 3-10 gli2b expression...

Ngày tải lên: 15/09/2015, 17:10

... affected in mib-/- embryos 11 1 Fig 3 -12 gli2b and Fgf3/8 11 3 Fig 3 -13 Validation of gli2b MO for blocking translation of zebrafish 11 6 gli2b Fig 3 -14 gli2b MO blocks zebrafish gli2b mRNA splicing 11 7 ... Concluding remarks 13 1 13 4 13 6 13 6 13 9 14 3 14 6 14 8 15 7 15 9 16 1 16 3 16 5 16 6 16 7 17 0 Chapter V Conclusion 17 3 Chapter VI References 17 5...

Ngày tải lên: 15/09/2015, 17:10

... Amino acid sequence alignment of Zebrafish, Human, Mouse and Chick ColIXα2 88 Phylogenetic tree of FACIT family comprising of chains of collagen IX, collagen XII, collagen XIV and collagen XIX ... chains of zebrafish type IX collagen and type II collagen alpha in the tail bud during segmentation and post-segmentation period 106 Translation inhibition of col...

Ngày tải lên: 15/09/2015, 22:06

... overexpression of Rab31 in breast cancer cell lines actually enhanced proliferation [112], Rab31, when elevated in the presence of MUC1-C may enhance instead of retard EGFR signalling in these cells Another ... as the lysosome inhibitor chloroquine increased MUC1-C levels in Rab31 silenced cells How then does Rab31 diminish late endososome-lysosome targeting of MU...

Ngày tải lên: 22/09/2015, 15:17

... with the patterns of binding of Nrl to the rhodopsin promoter (Fig 4C,D) The mRNA transcript levels of Ppp2r5c and rhodopsin in mouse retina corresponded to the observed increase in Nrl binding ... Regulation of beta-catenin signaling by the B56 subunit of protein phosphatase 2A Science 283, 2089–2091 16 Li X, Yost HJ, Virshup DM & Seeling JM (2001) Protein...

Ngày tải lên: 06/03/2014, 02:20

... characterization of heme-interacting proteins in the malaria parasite, Plasmodium falciparum J Biol Chem 278, 27354–27361 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of ... FP–agarose, but the binding was not affected by preincubation of the protein with FP These results also indicate that Pf1-Cys-Prx can bind to FP at pH 7.4,...

Ngày tải lên: 07/03/2014, 17:20

... that the adaptive immune system differentially modulates de novo cancer development in an organ-dependent and aetiology-dependent manner The paradoxical influence of the adaptive immune system during ... to be resolved with these powerful approaches is how therapeutically manipulating one arm of the immune system affects the anticancer or cancer- promoting p...

Ngày tải lên: 15/03/2014, 01:20