The role of microRNAs in embryonic stem cell development and differentiation
The role of microRNAs in embryonic stem cell development and differentiation
... as in embryonic carcinoma (EC) cells, which may have conserved roles in mammalian pluripotent stem cells (2) microRNAs expressed specifically in ESCs but not in other cells including EC cells These ... putative minimal promoter The number and precise sequences of the pre -microRNAs, their distance from the promoter and the polyadenylation sites, the regions flank...
Ngày tải lên: 12/09/2015, 08:16
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 2
... (m, 22 H), 0. 92 (t, 3H); 13C NMR (300 MHz, C6D6) δ 154.8, 94.6, 85.6, 80 .2, 79.7, 79 .2, 65.0, 64 .2, 63.8, 63 .2, 62. 8, 61.9, 32. 0, 29 .8, 29 .6, 29 .4, 29 .2, 28 .9, 28 .7, 28 .0, 25 .8, 25 .4, 23 .2, 22 .7, ... 64.44% 72. 98% 63.88% Structures of six obtained compounds (analogues of sphingosine) 2. 2 .2 Compounds Inhibitory Function on SPHK Activity in vitro 2. 2...
Ngày tải lên: 11/09/2015, 16:06
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 3
... 3. 1 .3 Staining 3. 1 .3. 1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the ... difference compared with the induced cells without inhibitors Interestingly and similarly with the hFOB test, 1μM of DMS and 5μM of CP6 inhibited the differentiatio...
Ngày tải lên: 11/09/2015, 16:06
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4
... penetrating the cells and act on, as yet unknown, intracellular targets capable of triggering GPCR-independent calcium signals 4. 2.3 .4 MAPK (P42 /44 ) Activation and IκB Phosphorylation MAPK (P42 /44 ) ... was added into the cells and incubated for different times The results are summarized in Figure 4. 13A, Figure 4. 13C, and Figure 4. 13D 146 Chapter Role of S1P...
Ngày tải lên: 11/09/2015, 16:06
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 5
... suggests the potential for the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including ... osteogenic differentiation in most of the cases, while at higher amounts (1μM DMS and 5 M CP6) showed to inhibit the osteogenic differentiation in mo...
Ngày tải lên: 11/09/2015, 16:06
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 6
... messenger in cell proliferation induced by PDGF and FCS mitogens." Nature 365 (64 46) : 557 -60 Olivera, Ana, Nicole Urtz (20 06) “IgE-dependent Activation of Sphingosine Kinases and and Secretion of Sphingosine ... N, N-dimethyl-derythro -sphingosine- induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Ot...
Ngày tải lên: 11/09/2015, 16:06
Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation
... clinical usage Meeting this increasing need for more MSCs is one of the main motivations of the present study, in particular, in studying the role of S1P in adult stem cell proliferation Another ... maintenance and differentiation In particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness mainte...
Ngày tải lên: 11/09/2015, 16:06
Investigation of the role of microRNAs in spinocerebellar ataxia type 3
... 19 -33 Ataxia type (SCA1) Spinocerebellar Ataxia type (SCA2) Spinocerebellar Ataxia type (SCA3) Spinocerebellar Ataxia type (SCA6) calcium channel subunit Spinocerebellar ATXN7 Ataxin 4 -35 37 -30 6 ... interspersed by introns that are omitted in the ATXN3 mRNA (b) The initial exons code for the Josephin domain in the ATXN3 protein, the rest of the e...
Ngày tải lên: 26/11/2015, 10:00
báo cáo khoa học:" The role of apoptosis in early embryonic development of the adenohypophysis in rats" ppsx
... cup-like indentation of the pharyngeal roof and, hence, originates from the ectoderm Regarding the development of the remaining cranial area, it may be mentioned that the development of the primary ... vis-à-vis, (V), 20× H&E staining of paraffin section of cranial part of the adenoH&E staining of paraffin section of cranial part of the adenohypophysis...
Ngày tải lên: 12/08/2014, 01:22
Báo cáo y học: " The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures" potx
... source of IFN-γ in the adaptive immune response [2,3] IFN-γ- inducible protein 10 (IP -10) is induced by IFN-γ in many types of cells including monocytes and lung epithelial cells [4,5] IP -10, also ... haematology analyzer to determine total cell number and the purity of the cell preparation This method typically yields a cell suspension containing 80–...
Ngày tải lên: 12/08/2014, 15:21
THE ROLE OF CERAMIDES IN CIGARETTE SMOKE-INDUCED ALVEOLAR CELL DEATH
... part of the great lab team iv Abstract Krzysztof Kamocki THE ROLE OF CERAMIDES IN CIGARETTE SMOKE-INDUCED ALVEOLAR CELL DEATH The complex pathogenesis of emphysema involves disappearance of alveolar ... apoptosis in endothelium of intestine, lung, fat and thymus Interestingly, the apoptosis was inhibited in the endothelium by administration of TNF-b...
Ngày tải lên: 24/08/2014, 09:42
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... specification of midbrain dopamine neurons Radial glia cells are neuronal progenitors in vivo 10 11 14 1. 3 Long non- coding RNAs in biology 15 1. 3 .1 1.3.2 1. 3.2 .1 1.3.2.2 1. 3.2.3 Long non- coding RNAs in pluripotency ... pluripotency, and repressing differentiation simultaneously 18 1. 3.2 Long non- coding RNAs in neural developmen...
Ngày tải lên: 09/09/2015, 17:54
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 2
Ngày tải lên: 09/09/2015, 17:54