Development of sphingosine kinase (SPHK) inhibitors and the role of sphingolipids in adult stem cell proliferation and differentiation 4

... (m, 22 H), 0. 92 (t, 3H); 13C NMR (300 MHz, C6D6) δ 154.8, 94.6, 85.6, 80 .2, 79.7, 79 .2, 65.0, 64 .2, 63.8, 63 .2, 62. 8, 61.9, 32. 0, 29 .8, 29 .6, 29 .4, 29 .2, 28 .9, 28 .7, 28 .0, 25 .8, 25 .4, 23 .2, 22 .7, ... 64.44% 72. 98% 63.88% Structures of six obtained compounds (analogues of sphingosine) 2. 2 .2 Compounds Inhibitory Function on SPHK Activity in vitro 2. 2...

Ngày tải lên: 11/09/2015, 16:06

... 3. 1 .3 Staining 3. 1 .3. 1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the ... difference compared with the induced cells without inhibitors Interestingly and similarly with the hFOB test, 1μM of DMS and 5μM of CP6 inhibited the differentiatio...

Ngày tải lên: 11/09/2015, 16:06

... penetrating the cells and act on, as yet unknown, intracellular targets capable of triggering GPCR-independent calcium signals 4. 2.3 .4 MAPK (P42 /44 ) Activation and IκB Phosphorylation MAPK (P42 /44 ) ... was added into the cells and incubated for different times The results are summarized in Figure 4. 13A, Figure 4. 13C, and Figure 4. 13D 146 Chapter Role of S1P...

Ngày tải lên: 11/09/2015, 16:06

... suggests the potential for the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including ... osteogenic differentiation in most of the cases, while at higher amounts (1μM DMS and 5 M CP6) showed to inhibit the osteogenic differentiation in mo...

Ngày tải lên: 11/09/2015, 16:06

... messenger in cell proliferation induced by PDGF and FCS mitogens." Nature 365 (64 46) : 557 -60 Olivera, Ana, Nicole Urtz (20 06) “IgE-dependent Activation of Sphingosine Kinases and and Secretion of Sphingosine ... N, N-dimethyl-derythro -sphingosine- induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Ot...

Ngày tải lên: 11/09/2015, 16:06

... clinical usage Meeting this increasing need for more MSCs is one of the main motivations of the present study, in particular, in studying the role of S1P in adult stem cell proliferation Another ... maintenance and differentiation In particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness mainte...

Ngày tải lên: 11/09/2015, 16:06

... as in embryonic carcinoma (EC) cells, which may have conserved roles in mammalian pluripotent stem cells (2) microRNAs expressed specifically in ESCs but not in other cells including EC cells These ... putative minimal promoter The number and precise sequences of the pre -microRNAs, their distance from the promoter and the polyadenylation sites, the regions flank...

Ngày tải lên: 12/09/2015, 08:16

... Retrovirology 2005, 2:29 Overexpression of CD30 in ATL cell lines We next examined CD30 expression in ATL-derived T -cell lines, HTLV-1 transformed cell lines and HTLV-1 negative T -cell lines using FACS ... binding protein pathways Virus Genes 2001, 22:279-287 Akagi T, Ono H, Shimotohno K: Expression of cell- cycle regulatory genes in HTLV-I infected T -cell...

Ngày tải lên: 13/08/2014, 09:21

... differences in severity of disease and times of disease onset depending upon the type of fats used for feeding Mice fed diets high in trans fats combined with high fructose in the drinking water ... RM, Yki-Järvinen H: Genes involved in fatty acid partitioning and binding, lipolysis, monocyte/macrophage recruitment, and inflammation are overexpressed in the h...

Ngày tải lên: 11/08/2014, 03:20

... differences in severity of disease and times of disease onset depending upon the type of fats used for feeding Mice fed diets high in trans fats combined with high fructose in the drinking water ... RM, Yki-Järvinen H: Genes involved in fatty acid partitioning and binding, lipolysis, monocyte/macrophage recruitment, and inflammation are overexpressed in the h...

Ngày tải lên: 11/08/2014, 06:22

... the theme: Formulating strategies for retail banking business of Joint Stock Commercial Bank for Investment and Development of Vietnam during the period 2013 - 2017 with the desire to apply ... for further development of the bank' s retail banking activities Subject and scope of the research: - Subject: Retail Activities at the...

Ngày tải lên: 26/03/2015, 10:58

... overall incidence of PTLD is - 2% The incidence of the disease in renal and liver transplant is – 5%, in heart and heart-lung transplant is – 15%, and in intestinal transplant is 10 – 15% 125 The incidence ... from the EBNA3A, B, C family of proteins and the frequency of specific cells comprise Humanized mouse model of EBV infection Introduction up t...

Ngày tải lên: 10/09/2015, 09:22

... 55 Fu Lei would later meet with the Sun brothers in France and holiday together Jin Mei, p 72 56 ‘Faxing tongxin shiwu pian’ in Fu Lei wenji: wenyijuan, pp 23-77 The series was later collated in ... Lee, ‘Incomplete Modernity: Rethinking the May Fourth Intellectual Project’ and Rudolf G Wagner, The Canonization of May Fourth’ in Milena Dolezelova-Velingerova and...

Ngày tải lên: 06/10/2015, 21:23

... program in Botswana maintains the hegemony and the gap between the poor and the rich, the major and minority groups In order to redress poverty the adult education program needs to be aware of the social ... vital in fighting poverty The adult education program can thus harness the local languages and indigenous knowledge of the minority and...

Ngày tải lên: 05/11/2012, 16:27