the drosophila ovary an in vivo stem cell system

... heavily involved in the production and regulation of all cell types in the biological system, including in haemopoiesis The identification of stem cells marked an important discovery in the field ... Enhanced understanding of the regulation of stem cell properties is critical for improving our ability to manipulate normal stem cells in vitro and in vivo Furthermore, understanding normal stem ... field of biology and medicine The haemopoietic system represents an important component in the stem cell time line, beginning with a variety of observations including the acceptance of donor bone...

Ngày tải lên: 22/12/2014, 21:11

... overall incidence of PTLD is - 2% The incidence of the disease in renal and liver transplant is – 5%, in heart and heart-lung transplant is – 15%, and in intestinal transplant is 10 – 15% 125 The incidence ... class I and class II antigen processing and presentation pathways22 The glycine-alanine repeats within the EBNA1 protein are proposed to inhibit degradation by the ubiquitin-proteasome system and ... viremia in the germinal centers in infectious mononucleosis causes the infection of bystander germinal center cells, pushing them into the latency III growth program and these constitute the majority...

Ngày tải lên: 10/09/2015, 09:22

... Aprotinin, 1mM Leupeptin and 1mM Pepstatin A The buffer was stored at 4℃ and the protease inhibitors (Aprotinin, Leupeptin, Pepstatin A, PMSF and SOV) were freshly added each time before using ... PhD study, and the requirement of Graduate Program in BioEngineering, investigations of the synthetic compounds were more on their applications in tissue engineering, which is in stem cells differentiation, ... dye, causing a 39 Chapter2 Development and Evaluation of Human SPHK Inhibitors visible color change Within the linear range of the assay (~5-25μg/mL), the more protein present, the more the Coomassie...

Ngày tải lên: 11/09/2015, 16:06

... staining in each sample was quantified by dissolving the staining with 100% of isopropanol and detecting the absorbance at 500nm 3.1.3.3 Fluorescence Immunostaining Fluorescence immunostaining ... Staining 3.1.3.1 Alizarin Red S Staining Alizarin Red S staining is widely used to evaluate the calcium-rich deposits generated by cells In this study, the staining was used to detect the mineralization ... removed and the cells were washed three times with distilled water Alizarin Red S staining in each sample was quantified by dissolving the staining with diH2O and detecting the absorbance under...

Ngày tải lên: 11/09/2015, 16:06

... placing the 6-well plates on ice and the cells were scraped out and collected by centrifugation Cell pellets were lysed and the protein concentrations in the cell lysates were quantified The ... would be convincing that the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally ... S1P in human BM- and AD- MSCs proliferation proliferation, as well as maintaining their stemness However, human adult stem cells, such as MSCs, are very different with embryonic stem cells in...

Ngày tải lên: 11/09/2015, 16:06

... for the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including in vivo ... further confirm our conclusions Finally, the study could be expanded to study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors ... validate the potential for SPHK inhibitors in adult -stem cell differentiation In the final part of my research project (Chapter 4), the bioactive lipid S1P was studied for its role in promoting human...

Ngày tải lên: 11/09/2015, 16:06

... roles of sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells." Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the treatment ... "Physiological and pathological actions of sphingosine 1-phosphate." Semin Cell Dev Biol 15(5): 513-20 Hla, T and T Maciag (1990) "An abundant transcript induced in differentiating human endothelial cells ... "Signalling mechanisms in sphingosine 1-phosphatepromoted mesangial cell proliferation." Genes Cells 7(12): 1217-30 Kee, T H., P Vit, et al (2005) "Sphingosine kinase signalling in immune cells." Clin...

Ngày tải lên: 11/09/2015, 16:06

... maintenance and differentiation In particular, we are interested in the role of S1P in human adult stem cell proliferation and stemness maintenance, and how SPHK inhibition would affect human ... significance for the need to study the potential applications and significance of using SPHK and S1P in stem cells research, derived from the current understanding on human stem cells and the strategies ... along a certain lineage The main advantage of using co-culture systems is that it allows intimate contacts between different cells, found in an organ, providing an efficient way in creating a more...

Ngày tải lên: 11/09/2015, 16:06

... miR-1 in muscles, miR-124 in the central nervous system and miR-10 in anterior-posterior patterning (Kloosterman and Plasterk, 2006) These observations indicate that microRNAs may be involved in the ... Seng, Huibin, Yin Loon, Minh, Sandy, Phil and all other past and present members of Bing’s lab, thank you for your friendship, and for making the lab such a stimulating and fun place to work in I ... new insights into these mechanisms have been gained via the use of cellfree in vitro systems (Mathonnet et al., 2007; Thermann and Hentze, 2007; Wakiyama et al., 2007; Wang et al., 2006) Wang...

Ngày tải lên: 12/09/2015, 08:16

... between the various components However, these in vitro experiments may not yield the in vivo mechanism of the regulatory system An experimental determination of the in vivo mechanism in a genetic ... parameters such as the binding constants and the extent of autoregulation The regulatory proteins may reside either in the nucleus or in the cytoplasm or in both, thus controlling the network The existence ... binding coefficients Further results from studies in molecular biology and bioinformatics can limit the number of candidate models Finally, the relevance of the different mechanisms at the system...

Ngày tải lên: 07/03/2014, 16:20

... remove cell contamination, and the supernatant was then stored at -80°C Tissue Processing for histology and immunohistochemical staining Upon completion of the in vivo monitoring, rats were anesthetized ... participated in its design and coordination and helped to draft the manuscript All authors read and approved the final manuscript Competing interests The authors declare that they have no competing interests ... gelatin sponge, Page of 10 the temporalis and overlying skin were sutured Animals were then placed in the supine position and a midline incision was made in the neck The bilateral common carotid...

Ngày tải lên: 18/06/2014, 16:20

... among the five strains There are nine unique amino acids in the RNase/ RT of BLV ARG 38 relative to all of the other BLV strains, including BLV ARG41 There are no differences between the integrases ... indicated by asterisks Only a portion of the BLV A Tax is shown, and the rest is indicated by the an wavy lines In the Env proteins the N-linked glycosylation sites are shown in bold, while the ... indicated by asterisks Only a portion of the BLV A Tax is shown, and the rest is indicated by the an wavy lines In the Env proteins the N-linked glycosylation sites are shown in bold, while the...

Ngày tải lên: 12/08/2014, 04:21

... among the five strains There are nine unique amino acids in the RNase/ RT of BLV ARG 38 relative to all of the other BLV strains, including BLV ARG41 There are no differences between the integrases ... indicated by asterisks Only a portion of the BLV A Tax is shown, and the rest is indicated by the an wavy lines In the Env proteins the N-linked glycosylation sites are shown in bold, while the ... indicated by asterisks Only a portion of the BLV A Tax is shown, and the rest is indicated by the an wavy lines In the Env proteins the N-linked glycosylation sites are shown in bold, while the...

Ngày tải lên: 12/08/2014, 04:22

... [36,37] The current hypothesis involves domain–domain interactions between the N-terminus and other domains of the ATPase Indeed, in vitro studies of the purified N-terminus and catalytic loop of the ... initially delivered by Atx1 and bound to the Ccc2 N-terminus, is next transferred to another binding site in the protein through domain–domain interactions Interestingly, a recent report on in ... that these two domains interact in the absence of copper and that their interaction is diminished by copper binding to the N-terminus [38] Such a domain–domain interaction has been proposed to hinder...

Ngày tải lên: 23/03/2014, 05:22

... developed intestinal obstruction after ingesting surgical dressing and was euthanized days before schedule The femora were harvested from this rabbit and included in the SEM analysis The details ... harvested, cleaned of soft tissue, and fixed in 70% ethanol The femur bone was trimmed above and below the ends of the implant, cleaned of soft-tissue, and fixed in 70% alcohol The specimen was further ... (with an acid resistant mask) and chemical milling with nitric and hydrofluoric acid The details regarding the chemical texturing process and the osseointegration of chemical-textured implants...

Ngày tải lên: 20/06/2014, 04:20

... stromal cells and likely other cells in the body Since osteoblast (a cell derived from mesenchymal stem cells) is a key component in the HSC niche for the Advances in Hematopoietic Stem Cell Research ... developmental windows since each decision can be uncoupled in vivo is very attractive The finding that both aorta and vein express HSC markers in the Notch-activated state with minimal change in ephrinB2a ... stimuli trigger intrinsic determinants of cell fate, the transcription factors which contribute to the reprogramming of HSCs into cell- Advances in Hematopoietic Stem Cell Research lineage restricted...

Ngày tải lên: 27/06/2014, 13:20

... determined by dividing the density of the released DNA band by the density of the total DNA in the lane (the released DNA band plus the unreleased DNA remaining in the well) Page of 12 Cell Cycle Analysis ... documentation system (Alpha Innotech, San Leandro, CA) and AlphaEaseFC software (Alpha Innotech, San Leandro, CA) Each band in the gel was outlined manually and the density determined The results ... loratadine alone results in Chk1 activation leading to an increase in the percentage of cells in the G2/M phase of the cell cycle Since Page of 12 the G2/M phase of the cell cycle is one of the...

Ngày tải lên: 09/08/2014, 10:20

... leading to self injury Besides HSCs, there also existed another kind of stem cells called MSCs (Mesenchymal Stem Cells), they could differentiated into stroma cells and acted as the “niche” in the ... secreted by the tumor cells, and the transfer and proliferation of tumor cells[36] So, the loose of ECM and secreted cytokines are important for the metastasis of the tumor cells from the primary ... the manuscript AG carried out the immunoassays SY participated in the design of the study and performed the statistical analysis All authors read and approved the final manuscript Competing interests...

Ngày tải lên: 10/08/2014, 10:21

... Submicrometric and nanometric topography determine cell reactions including cell orientation, changes in cell motility, cell adhesion and cell shape Therefore these topographic features play an important ... placed in the tibia The implant sites were sequentially enlarged with two drills according to the standard protocol of the manufacturer Implants measuring mm in length and 3.5 mm in diameter were inserted ... Germany) Anesthesia was induced with an intravenous bolus of 3–5 ml thiopental (Thiopental inresa®, Inresa Arzneimittel, Freiburg, Germany) followed by intubation and maintenance of anaesthesia...

Ngày tải lên: 11/08/2014, 20:20