Proteomics analysis of pro inflammatory cytokine stimulated human lung fibroblasts and bronchial epithelial cells

... global protein profilings of normal human bronchial epithelial cells (NHBE) and normal human lung fibroblasts (NHLF) stimulated with pro- inflammatory cytokines tumor necrosis factor (TNF)-α and/ or ... contribution of two major airway resident cells, bronchial epithelial cells and lung fibroblasts, to the inflammatory events will be reviewed and disc...

Ngày tải lên: 11/09/2015, 16:05

... further investigate the role of Nurr1 in regulating inflammatory processes in synovial cells, and by using transcript profiling to identify Nurr1 regulated genes in synoviocytes, which may be playing ... capable of sharing overlapping roles, in primary synoviocytes, treatment with various cytokines shows predominantly Nurr1 upregulation [20] Therefore in rheumatic synovi...

Ngày tải lên: 11/08/2014, 08:21

... disease are more potent than human H1N1 virus at inducing proinflammatory cytokines and chemokines, including IP-10, interferon beta, IL-6 and RANTES, from human primary alveolar and bronchial epithelial ... interleukin and interferon beta proteins in the primary human bronchial and alveolar epithelial cell supernatants were measured by a specific ELISA...

Ngày tải lên: 12/08/2014, 18:20

... capable of interacting with T-lymphocytes, we set out to determine whether the interaction between normal human lung fibroblasts and T-cells could lead to a functionally significant response by T-lymphocytes, ... T-lymphocytes, influencing their state of immune activation Our results indicate that lung fibroblasts and T-lymphocytes indeed mutually interact Activat...

Ngày tải lên: 12/08/2014, 18:22

... study the effect of tumor necrosis factor-alpha (TNF-α) on humanjoint capsule myofibroblasts Seven The experimental setup setup to the effect of tumor necrosis factor-alpha (TNF-α) on human joint ... http://arthritis-research.com/content/12/1/R4 Page 11 of 16 Figure The tumor tumor necrosis factor-alpha (TNF-α) on collagen contraction The eff...

Ngày tải lên: 12/08/2014, 11:22

... osteoclast formation and activation, and increased expression of RANK/ RANKL in inflammatory cells, in inflamed synovium, in articular cartilage and at the invading front of bone erosions It has ... 2):ii32-ii39 Takayanagi H, Iizuka H, Juji T, Nakagawa T, Yamamoto A, Miyazaki T, Koshihara Y, Oda H, Nakamura K, Tanaka S: Involvement of receptor activator of nuclear factor k...

Ngày tải lên: 09/08/2014, 06:22

... in levels of transcripts encoding enzymes in the kynurenine pathway in human skinderived fibroblasts following treatment with IFN-g (white), TNFa (gray) or the combination of these two cytokines ... enzymes were investigated; Indoleamine 2,3-dioxygenase (IDO1), Tryptophan 2,3dioxygenase (TDO), Kynurenine aminotransferases (KAT) 1-4, Kynurenine 3-monooxygenase (KMO),...

Ngày tải lên: 11/08/2014, 03:20

... in levels of transcripts encoding enzymes in the kynurenine pathway in human skinderived fibroblasts following treatment with IFN-g (white), TNFa (gray) or the combination of these two cytokines ... enzymes were investigated; Indoleamine 2,3-dioxygenase (IDO1), Tryptophan 2,3dioxygenase (TDO), Kynurenine aminotransferases (KAT) 1-4, Kynurenine 3-monooxygenase (KMO),...

Ngày tải lên: 11/08/2014, 06:23

... profile of release of the cytokines most reportedly elevated in COPD patients during exacerbations Figure of Severity COPD influences cytokine release Severity of COPD influences cytokine release ... the cytokine networks involved in an exacerbation have remained unexplored The majority of clinical studies have previously concentrated on examining the acute inf...

Ngày tải lên: 12/08/2014, 15:21

... group of GHMP kinases in which proton transfer does not play a major role in the rate determining step of catalysis That five of the 10 disease-causing mutations resulted in insoluble protein in ... play a major role in the rate-determining step of catalysis Several of the disease-causing mutations are not soluble following induction in E coli Although all the mut...

Ngày tải lên: 08/03/2014, 02:20

... Analysis of signaling networks using protein arrays H Voshol et al a contextual understanding of the molecular mechanisms of disease, but also has the potential to facilitate the validation of ... possible Arguably, signaling pathways are currently the best practical translation of ‘physiology’ because Analysis of signaling networks using protein arrays t...

Ngày tải lên: 16/03/2014, 00:20

... Timson Disease-causing mutations in human GALE Fig Crosslinking of wild-type and disease-causing mutants of UDP-galactose 4-epimerase (GALE) Crosslinking was carried out with N-(3dimethylaminopropyl)-N¢-ethylcarbodiimide ... during purification Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the nine disease-causing m...

Ngày tải lên: 23/03/2014, 11:20

... refer to the sequences that are present in the genome of interest and absent from the host genome as being ‘host-blind’ (human- blind, mosquito-blind, mouseblind, rat-blind, etc.) sequences The greater ... (22-mers) of the viral genome is comprised of n-mers that are not present in any of the other dengue genomes For example, in the genome o...

Ngày tải lên: 23/03/2014, 11:20

... in mϕ Release of HMGB1 contributes to Fas-induced proinflammatory cytokine production by mϕ Both Fas and HMGB1 have previously been shown to induce pro-inflammatory cytokine production by mϕ[1014,19] ... activation of Fas induces not only active but also rapid release of HMGB1 from viable mϕ via a TLR4/IRAK4-dependent mechanism Furthermore, the data from this study s...

Ngày tải lên: 11/08/2014, 03:20

... receptors including the glucocorticoid receptor α (GRα) [10-15] HDACs function by deacetylating of key components of the transcriptional machinery including the core histone proteins resulting in their ... et al., Cigarette Smoke Exposure Alters mSin3a and Mi-2?/? Expression; implications in the control of pro-inflammatory gene transcription and gl...

Ngày tải lên: 11/08/2014, 03:20