analysis of line 1 expression in human pluripotent cells

... kcat/Km,gal (LÆmol )1 s )1) kcat/Km,ATP (10 5 · LÆmol )1 s )1) Wild-type H44Y R68C A198V G346S G347S G349S 8.7 2.0 3.9 5.9 0.4 1. 1 1. 8 970 7700 430 660 11 00 13 000 17 00 34 13 0 11 0 26 89 39 8900 270 11 000 8500 ... galactokinase Biochem J 10 1, 70–75 19 Walker, D.G & Khan, H.H (19 68) Some properties of galactokinase in developing rat liver Biochem J 10 8, 16 9 17 5 20 Gulbinsky, J.S & Cleland, W.W (19 68) Kinetic ... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 26 Wang, W & Malcolm, B.A (19 99) Two-stage PCR protocol allowing introduction of...

Ngày tải lên: 08/03/2014, 02:20

... K257R L 313 M G 319 E R335H 69 82 93 16 0 14 0 97 66 35 78 99 36 32 0.046 1. 1 5.0 11 5 .1 5.8 30 15 ± ± ± ± ± ± ± ± ± ± 12 15 24 38 21 40 15 11 13 12 ± ± ± ± ± ± ± ± ± ± kcat ⁄ Km (LÆmol )1 s )1) 1. 4 1. 3 ... 0.23 1. 2 0.29 0.36 1. 3 0.48 5.2 3.9 5.0 6.9 3.6 1. 1 7.8 1. 7 3.9 1. 5 ± ± ± ± ± ± ± ± ± ± 0.72 · 10 5 0.59 · 10 5 0 .11 · 10 2 1. 2 · 10 3 0.40 · 10 4 0.35 · 10 5 1. 5 · 10 4 0.46 · 10 5 0.53 · 10 5 0 .14 · 10 5 ... during purification Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the nine disease-causing mutant proteins were determined (Table 1) In general, little...

Ngày tải lên: 23/03/2014, 11:20

... stim- Page 15 of 16 (page number not for citation purposes) Journal of Inflammation 2008, 5:8 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 ulated human bronchial epithelial cells are ... preconditioning (1andminfor 24 h) singly and with (B) in NHBEHRV-1b infection (TCID50 10 2.5 for h; 0mins) on mICAM -1 protein expression (A) ng/ml inoculation expression HRV -14 or cells over the ... repEffect of Effect of IFN-γ cell preconditioning (1 ng/ml for 24 h) singly and with HRV -14 or HRV-1b infection (TCID50 10 2.5 for 90 mins) on mICAM -1 protein expression (A) and mICAM -1 gene expression...

Ngày tải lên: 11/08/2014, 08:22

... CDM (AI 34749) References 10 11 12 13 14 15 16 17 18 19 Marquet R, Isel C, Ehresmann C, Ehresmann B: tRNAs as primer of reverse transcriptases Biochimie 19 95, 77 :11 3 -12 4 Mak J, Khorchid A, Cao ... Infections were initiated with transfection supernatant containing approximately 200 pg of p24 antigen in a volume of 10 mLs of media, giving a final p24 level of 20 pg/mL on day zero At 14 day intervals, ... replication of these viruses in PBMC Infections were initiated with 200 pg of p24 and were allowed to proceed with re-feeding of PBMC every 14 days for periods of time exceeding 50 days of in vitro...

Ngày tải lên: 13/08/2014, 09:21

... 0 .14 3 0 .17 7 0.483 0.4 Bm 17 9827 81 Citrate synthase 11 2 11 4 C No No No 0 .14 6 0 .14 6 0.576 0.493 1. 2 Bm 17 983059 Arginyl-tRNA synthetase 11 1 11 3 C No No No 0.057 0.057 0.225 0 .19 2 1. 2 Bm 17 98 219 6 ... Microbes Infect 19 99, 1: 1 211 -12 19 Teyssier C, Marchandin H, Simeon De Buochberg M, Ramuz M, Genome Biology 2007, 8:R 110 http://genomebiology.com/2007/8/6/R 110 10 12 13 15 16 17 19 21 22 28 29 30 31 ... isomerase 13 1 224 C No No No 0.089 0 .11 9 0.3 51 0.408 0.9 Bm 17 98 213 8 ATP synthase F1, alpha chain 15 8 11 2 U No No No 2.63 2.63 10 .370 8.880 1. 2 Bm 17 98 214 1 ATP synthase F1, beta chain 18 0 17 3 U No...

Ngày tải lên: 14/08/2014, 07:21

... naive T cells [40] Dendritic cells are involved in the polarization of the immune response towards a Th1 (large production of interferon-c) or a Th2 (sustained production of interleukins-4 and ... AH007340) by including the introns 3 216 –3305, 3 413 –35 41, 3633–3722, 3839–3930 and 4 013 – 4205, respectively In the presence of contaminant genomic DNA, the expected size of the amplicons would be 10 62 ... in both human immature dendritic cells and in rat Ó FEBS 2002 The endocannabinoid system in human dendritic cells (Eur J Biochem 269) 3775 Fig FAAH, CB1 and CB2 mRNA expression in dendritic cells...

Ngày tải lên: 22/02/2014, 07:20

... GATA exon Bgl II 10 01 1050 11 00 11 50 12 00 Sty I p1 12 50 13 00 13 50 14 00 14 50 15 00 5'-A [20] Sp1 GATA Ap -1 GATA Inr GATA Sp1 exon Sty I Hinc II 15 01 1550 16 00 BamH I 16 50 17 00 In order to clone ... [20] Sp1 Sp1 Sp1 750 800 850 900 950 10 00 C .1 Sp1 GATA GATA Sp1 exon Sac I 10 01 1050 GATA 11 00 GATA Sp1 ets -1 Sp1 12 00 12 50 13 00 13 50 14 00 CREB GATA Arnt 14 50 Sp1 15 00 12 3 12 0 48 C/EBP 12 3 20 12 ... Sma I 11 50 15 8 85 C.2 C.3 C.4 5'-B [20] Sp1 AhR/Arnt Bgl II 513 6 48 50 10 0 15 0 200 10 00 6000 Normalized Luciferase Activity (mV) 15 01 1550 Oct -1 1600 GATA 16 50 17 00 17 50 18 00 Sp1 18 50 C/EBP 19 00...

Ngày tải lên: 08/03/2014, 16:20

... and expression (Eur J Biochem 2 71) 10 61 (19 .5 ± 3 .1% ), ET -1 in the serum of hypertensive patients is no more than 13 % above normal [40] Thus, an increase in ET -1 by this margin could be one of ... were interested in investigating the effects of ouabain on cytosolic [Ca2+] of HUAECs As shown in Fig 1B, ouabain at the low concentration of nM induced clear Ca2+ oscillations in these cells ... counting The 86Rb+ activity was normalized against the amount of protein [13 ] per well 86 Rb+ uptake was also investigated after preincubating the cells for 15 with lM protein kinase C inhibitor...

Ngày tải lên: 30/03/2014, 13:20

... expressed in individual F -11 cells fits well with this result In F -11 parental cell line N18TG2 we detected the expression of mouse VRL -1 due to its murine origin Therefore we propose that in the F -11 ... P7 61 and a stop codon was introduced (pcDNA3 .1/ rVRL -1) Ó FEBS 2003 Co -expression of mouse and rat VRL -1 in F -11 cells (Eur J Biochem 270) 4267 VRL -1 is expressed in F -11 cells We probed an F -11 ... VRL -1 in F -11 cells (Eur J Biochem 270) 4269 anti-VRL -1 polyclonal antibody gives only weak signals in the cytoplasm of F -11 cells (Fig 7A,B) In contrast rVRL -1 transiently expressed in F -11 cells...

Ngày tải lên: 31/03/2014, 07:20

... 10 1 (37/64) 0.0 01 0.999 GO:0006 512 Ubiquitin cycle 473 11 9 (86/33) 0.000 1. 000 0.999 GO:0 016 567 Protein ubiquitination 256 60 ( 41/ 19) 0.002 GO:0006 511 Ubiquitin-dependent protein catabolism 10 4 ... gene expression patterns in postmortem human brains Study of expression levels of 14 genes in human brain autopsy and biopsy samples found significant change in one of the genes, indicating that ... (23 /13 ) 0.000 1. 000 GO:0006396 RNA processing 3 41 118 (64/54) 0. 011 0.995 GO:0006397 mRNA processing 217 74 (44/30) 0.002 0.999 GO:0008380 RNA splicing 18 3 67 (39/28) 0.000 1. 000 GO:00062 81 DNA...

Ngày tải lên: 14/08/2014, 16:20

... al LPS increases ICAM -1 expression in Schwann cells of sciatic nerves A B C D Fig LPS induced the expression of ICAM -1 protein in cultured SCs (A) LPS induced ICAM -1 protein expression in a concentration-dependent ... 57, 10 1– 10 9 17 Sobel RA, Mitchell ME & Fondren G (19 90) Intercellular adhesion molecule -1 (ICAM -1) in cellular immune reactions in the human central nervous system Am J Pathol 13 6, 13 09 13 16 18 ... immunostaining using ICAM -1 antibody with NF-200 (specific to neurofilaments), S100 (specific to Schwann cells) and CD 31 (a marker of endothelial cells) In previous studies, ICAM -1 integrin interactions...

Ngày tải lên: 18/02/2014, 18:20

... Cystatin F in U937 cells (Eur J Biochem 269) 5 511 18 19 20 21 22 23 24 25 26 27 28 precursor of the human cysteine proteinase inhibitor cystatin C FEBS Lett 216 , 229–233 Hultberg, B., Lindsten, ... be of relevance for expression in U937 and native haematopoietic cells Fig Regulation of cystatin expression in TPA stimulated U937 cells Cells were incubated for days in the presence of 0 .13 ... to be involved in invariant chain [8] and antigen [11 ] processing, respectively Our present results showing a distinct intracellular localiza- tion of cystatin F are interesting in this context...

Ngày tải lên: 21/02/2014, 01:21

... assisting the binding to raptor: the first 17 amino acids [compare the signal for full-length 4E-BP1 (1 11 7) with that for the 18 11 7’ variant] and sections of the C-terminal half of 4E-BP1 [compare, ... [19 ]) Previous work has shown that mutation of the phenylalanine to alanine in the TOS motif eliminates the binding of raptor to the C-terminal fragment of human 4E-BP1 in the overlay assay [19 ] ... mutating the proline to alanine (to give the RAIA mutant) causes a defect in the basal and insulin-stimulated phosphorylation of 4E-BP1 [25] We also tested the proline to valine mutation in wild-type...

Ngày tải lên: 16/03/2014, 06:20

... fluorescence intensity was 19 5 in the off-state and 18 60 in 786 Doxycycline – Relative background transcription (%) 0. 014 0. 014 0.0 011 0.0052 0.045 0.0 017 0.068 0.0077 0.0 31 0. 012 1. 4 1. 6 0.096 0.33 1. 9 ... for the production of the serotonin transporter Biochim Biophys Acta 16 10, 14 1 15 3 Gossen M & Bujard H (19 92) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters ... transactivator domains, such as VP16, interact with a variety of transcription factors [12 ,13 ] Indeed, analysis of expression levels in stably transfected HeLa cells suggests that in high numbers,...

Ngày tải lên: 23/03/2014, 09:21

... that Ets -1 ⁄ Elk -1- binding motifs are critical for DPP-III gene expression 200 bp x35.94 10 0 81 bp pAAS -1 pAAS -1 Mut1 pAAS -1 Mut2 Fig Functional relevance of Ets -1 ⁄ Elk -1- binding motifs in DPP-III ... promoter 18 70 The N-terminal domain of Ets -1 is involved in the formation of a complex with other proteins, whereas its C-terminal domain is involved in DNA binding [38] By contrast, the DNA-binding ... binding a heterodimer of Ets -1 and Elk -1 to the probe, and the N-terminus of Ets -1 and the C-terminus of Elk -1 were involved in the formation of the heterodimer Preincubation of nuclear proteins...

Ngày tải lên: 29/03/2014, 09:20

... Gauthier JM (19 98) Direct binding of Smad3 and Smad4 to critical TGF beta-inducible elements in the promoter of human plasminogen activator inhibitortype gene EMBO J 17 , 30 91 3 310 15 Vulin AI & Stanley ... Thymosin beta induces the synthesis of plasminogen activator inhibitor in cultured endothelial cells and increases its extracellular expression Blood 10 3, 13 19 13 24 17 Hou B, Eren M, Painter ... Immunol 12 , 14 1 11 7 37 Ruan QR, Zhang WJ & Hunfnagl P (20 01) Anisodamine counteracts lipopolysaccharide-induced tissue factor and plasminogen activator inhibitor -1 expression in human endothelial cells: ...

Ngày tải lên: 30/03/2014, 11:20

... median number of molecules per CD22+ cell in CB, PB and LP was 11 ,650 (range, 10 ,702 14 ,15 8), 17 ,490 (range, 13 ,772 19 ,997) and 9, 618 (range, 4,339– 10 ,774), respectively On CD19+ B -cells the median ... 1, 128–4,392) and 2, 913 (range, 1, 552– 4 ,13 3), respectively In addition, the T lymphoid cell line CRF-HSB-2 and the myeloid KG -1 cell line expressed 29,245 and 38 ,14 1 CD46 molecules per cell 1. 5 ... P: Human herpesvirus (HHV-6) Antiviral Res 19 96, 31: 1- 21 Seya T, Hara T, Matsumoto M, Akedo H: Quantitative analysis of membrane cofactor protein (MCP) of complement High expression of MCP on human...

Ngày tải lên: 20/06/2014, 01:20

... band in rats [13 ] In rats, AQP1 expression was clearly detected in spleen [13 ], though AQP1 expression in HK dog spleen was unpronounced To investigate AQP1 protein expression in various HK dog ... proteins in the spleen There was no significant difference in AQP1 expression between the HK and LK tissues examined (Fig 2A) In this report, we investigated the expression of AQP1 in canines ... in human erythroleukemia cell lines Genomics 19 98, 47, 3 41- 349 17 Yamamoto T, Sasaki S Aquaporins in the kidney: Emerging new aspects Kidney Int 19 98, 54, 10 41- 1054 ...

Ngày tải lên: 07/08/2014, 20:23

... staining); ( 21- 40% of the sample exhibiting staining); ( 41- 60% of the sample exhibiting staining); ( 61- 80% of the sample exhibiting staining), and ( 81- 100% of the specimen stained) The findings ... exhibiting staining); ( 214 0% of the sample exhibiting staining); ( 41- 60% of the sample exhibiting staining); ( 61- 80% of the sample exhibiting staining), and ( 81- 100% of the specimen stained) An immunohistochemical ... following neurosurgical resection J Neurosurg 19 85, 62 (1) :18 -24 Wernicke et al Radiation Oncology 2 010 , 5:46 http://www.ro-journal.com/content/5 /1/ 46 10 11 12 13 14 15 16 17 18 19 20 21 22 23...

Ngày tải lên: 09/08/2014, 09:20

... with mL of media containing 10 ng/mL of either human recombinant IL -1 (AMS Biotechnology Ltd, Abingdon, UK) or human recombinant Available online http://arthritis-research.com/content /11 /3/R65 ... 12 0 :11 3 -13 0 Wang JY, Baer AE, Kraus VB, Setton LA: Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture Spine 20 01, 26 :17 47 -17 51 discussion 17 52 ... (Figure 1a) There was no significant change in gene expression for MMP-9 in normal or degenerate cells or for MMP -13 in degenerate cells (Figure 1a) Interleukin -1- beta The addition of recombinant...

Ngày tải lên: 09/08/2014, 14:20