proteomics analysis of human nonalcoholic fatty liver

... for proteomics 35 Scheme of proteomics workflow and intruments 50 Differential protein profiling of TNF-α-stimulated NHLF 69 MS spectrum and Mascot search results of DCIP 73 RT-PCR analysis of ... MEDIATES TNF-α STIMULATION OF NHLF 3.1 Results 66 67 3.1.1 Proteomics analysis of DCIP induction by TNF-α 67 3.1.2 Verification of proteomics data by RT-PCR 75 3.1.3 Regulation of TNF-α-induced DCIP ... Principles of MS 33 1.6.2.2 Types of mass spectrometers 38 1.6.3 Protein database and protein identification 40 1.6.4 Application of proteomics 42 1.6.4.1 Expression proteomics 43 1.6.4.2 Structural proteomics...

Ngày tải lên: 11/09/2015, 16:05

... metaDQDO\VLVRIVWXGLHVZHIRXQGWKDWWKHVSHFL¿FLW\RI ultrasound for diagnosis of fatty liver was 94% when using liver biopsy as the gold standard(36) In conclusion, administration of silymarin can effectively reduce liver aminotransferases without ... Protective and therapeutic effect of Silymarin on the development of latent liver damage Radiats Biol Radioecol 1998;38:4115 Luper S A review of plants used in the treatment of liver diseases: Part Altern ... al Sampling variability of liver biopsy in nonalcoholic fatty liver disease Gastroenterology 2005;128:1898-906 Hajiaghamohammadi AA, Ziaee A, Oveisi S, Masroor H Effects of Metformin, Pioglitazone,...

Ngày tải lên: 10/06/2016, 16:46

... NAFLD Finantial support Conflicts of Interest The authors have declared that there is no conflict of interest References Ar ch ive of Angulo P Nonalcoholic fatty liver disease N Engl J Med 2002;346(16):1221-31 ... technical review on nonalcoholic fatty liver disease Gastroenterology 2002;123(5):1705-25 Marchesini G, Bugianesi E, Forlani G, Cerrelli F, Lenzi M, Manini R, et al Nonalcoholic fatty liver, steatohepatitis, ... hepatoprotective properties against a wide range of liver damage-inducing agents render MT a unique drug Milk thistle in liver steatosis Figure Structures of the components of silymarin SI D glucose levels,...

Ngày tải lên: 10/06/2016, 16:46

... 1: Analysis of total human RNA of RBCs (Bioanalyzer 2100 Agilent, USA): A) L- RNA 6000 ladder (Ambion, USA); 1-3 - total RNA of human RBCs from different donors; B) typical electropherogram of ... different donors; B) typical electropherogram of total RNA of human RBCs Microarray analysis of RNA from human RBCs Recent proteomic studies of RBCs based on 1D/2D-electrophoresis 6, or mass spectrometry ... mechanism of globin m-RNA stability in human RBCs Further experiments are needed to understand the mechanisms and the biological meaning of these findings But gene expression profiling of human erythrocyte...

Ngày tải lên: 03/11/2012, 10:58

... against NAFLD in humans, whereas another epidemiological study supported a correlation of the e4 allele with increased pathogenesis of fatty liver disease [34] As the human apoE2 isoform of apoE is ... are expressed as milligrams of triglycerides per gram of tissue ± SEM Histological analysis of liver samples At the end of the 24-week period, mice were sacrificed, and liver and visceral fat specimens ... metabolic abnormalities [12,13] Nonalcoholic fatty liver disease (NAFLD) is a spectrum of metabolic abnormalities ranging from simple accumulation of triglycerides in the liver (hepatic steatosis)...

Ngày tải lên: 05/03/2014, 23:20

... reproduction of this article is prohibited NONALCOHOLIC FATTY LIVER DISEASE HOSPITALIZATIONS IN CHILDREN 599 FIG Temporal trends in hospital discharges with mention of nonalcoholic fatty liver disease ... between nonalcoholic fatty liver disease prevalence and visceral fat in obese adolescents Dig Liver Dis 2008;40:132–9 25 Imhof A, Kratzer W, Boehm B, et al Prevalence of non-alcoholic fatty liver ... al Nonalcoholic fatty liver disease: an overview of current insights in pathogenesis, diagnosis and treatment World J Gastroenterol 2008; 14:2474 –86 Adams LA, Lindor KD Nonalcoholic fatty liver...

Ngày tải lên: 14/03/2014, 11:20

... modulation of regulatory networks [5,6] A direct benefit of such an approach would be the early recognition of ‘off target’ and side effects of drug candidates [7], as well as the identification of putative ... The availability of the human genome and the subsequent prediction of the ‘kinome’, the complete set of some 500 human kinases [18], have further accelerated the development of kinase panels ... comparative analysis of signaling pathway(s) events in normal versus diseased tissue, (b) the comparative analysis of protein expression in various systems, (c) elucidation of the dynamic aspects of pathway...

Ngày tải lên: 16/03/2014, 00:20

... Histopathology of pediatric nonalcoholic fatty liver disease Hepatology 2005;42:641-649 32 Angulo P Nonalcoholic fatty liver disease N Engl J Med 2002;346:1221-1231 33 Adams LA, Angulo P Role of liver ... of nonalcoholic steatohepatitis as a cause of advanced liver disease Liver Transpl 2001;7:608-614 80 McCullough AJ The clinical features, diagnosis and natural history of nonalcoholic fatty liver ... hepatic vein Nonalcoholic fatty liver disease in children 73 Adams LA, Sanderson S, Lindor KD, Angulo P The histological course of nonalcoholic fatty liver disease: a longitudinal study of 103 patients...

Ngày tải lên: 22/03/2014, 10:20

... follow-up of more than years for the last patient enrolled The diagnosis of NAFLD required (1) confirmation of diffuse fatty infiltration of the liver in imaging studies regardless of aminotransferase ... correlates of pediatric nonalcoholic fatty liver disease J Pediatr 2003;143:500–5 Schwimmer JB, McGreal N, Deutsch R, et al Influence of gender, race, and ethnicity on suspected fatty liver in ... prognosis of fatty liver: risk of chronic liver disease and death Gut 2004;53:750–5 Ekstedt M, Franzen LE, Mathiensen UI, et al Long-term follow-up of patients with NAFLD and elevated liver enzymes...

Ngày tải lên: 22/03/2014, 10:20

... algorithm based on simple analysis of correlation matrix is employed to reduce the dimensionality of features Further selection of feature set is based on experiments A multivariate analysis- based method ... amplitude of the ECG waveforms Due to its steep slopes, the frequency content of the QRS complex is considerably higher than that of the other ECG waves, and is mostly concentrated in the interval of ... we provide a review of the related works Biel et al [2] are among the earliest effort that demonstrates the possibility of utilizing ECG for human identification purposes A set of temporal and amplitude...

Ngày tải lên: 22/06/2014, 19:20

... distribution of the log10 of the total number of intronic nucleotides in the 5’UTR (b) Distribution of the number of introns in the 5’UTR The log10 of number of transcripts that have a given number of ... Page of 17 Figure Characterization of fundamental properties of 5’UTR introns (a) Histogram of the total 5’UTR intron length A well annotated set of RefSeq transcript IDs are used in this analysis ... distribution of these introns in different functional categories of genes Results Characterization of a set of genes with 5’UTR introns To investigate the functional properties of human 5UIs,...

Ngày tải lên: 09/08/2014, 20:21

... ClusterProfiler was designed to provide statistical analysis of GO and KEGG and visualization tools for comparing functional profiles among gene clusters More details on the use of clusterProfiler ... miRNAs not share a high level of homology even within the members of the same family [6,39] Phylogenetic analysis of all previously known virus miRNA genes showed that most of the known viral miRNAs ... 29 30 31 32 Zhu JY, Strehle M, Frohn A, Kremmer E, Hofig KP, Meister G, Adler H: Identification and Analysis of Expression of Novel MicroRNAs of Murine Gammaherpesvirus 68 J Virol 2010, 84:10266-10275...

Ngày tải lên: 10/08/2014, 09:22

... expression profiles of selected genes of interest that fit one of four different temporal expression categories Gene expression profiles of selected genes of interest that fit one of four different ... Examples of genes from each of these temporal categories are shown in Fig The cellular localization and functions of selected genes from each of these categories is shown in Table An analysis of the ... Results Exposure of human lung fibroblasts to V2O5 resulted in significantly altered expression of over 1400 genes on the Affymetrix Human Genome U133A 2.0 Array The majority of significantly...

Ngày tải lên: 12/08/2014, 15:20

... distribution of Lor or Gar was not contributing to their loss of activity Sequence analysis of Lor and Gar Tax proteins The sequences of Lor and Gar were determined and compared to that of Mo Lor ... reduced the ability of Mo to transactivate the LTR and without affecting NFkB activity (Table 3) Analysis of the subcellular location of mutant proteins in Cos cells using immunofluorescence did ... to be involved in the cytoplasmic location of Tax proteins [13,14,16,22] However analysis of the subcellular location of mutant proteins using immunofluorescence did not reveal any discernable...

Ngày tải lên: 13/08/2014, 09:21

... representative of the various cell types within the heterogeneous population of BCs We describe our method of assessing heterogeneous samples in three levels of analysis The first level consists of making ... reliability of the microarray data generated from our multi-comparison analysis is demonstrated by the consistent identification of a set of genes among multiple comparisons, of which a subset of genes ... reliability of the microarray data generated from our multi-comparison analysis is demonstrated by the consistent identification of a set of genes among multiple comparisons, of which a subset of genes...

Ngày tải lên: 14/08/2014, 08:20

... W, Johnson RM, Hof PR, Kapatos G, Grossman LI, Goodman M: Sister grouping of chimpanzees and humans as revealed by genome-wide phylogenetic analysis of brain gene expression profiles Proc Natl ... Neuroblastoma Chimpanzee (b) Cervical carcinoma Human Chimpanzee Brain Heissig et al R57.3 Liver Human Chimpanzee Human Chimpanzee ACADSB comment Human Volume 6, Issue 7, Article R57 UNG TERF1 ... Selection of promoters for study Genes for promoter analysis were selected on the basis of a large-scale transcriptome comparison between three humans and three chimpanzees in brain and liver using...

Ngày tải lên: 14/08/2014, 14:21

... Figure GoMiner analysis of the molecular function of identified proteins GoMiner analysis of the molecular function of identified proteins High-confidence and high-coverage analysis of the seminal ... tasks of the spermatozoa [31] The potential use of the proteomic data set for biomarker discovery Identification of disease biomarkers is an overarching aim of large proteomics studies of bodily ... the case of human seminal plasma, the aim would be the discovery of new biomarkers for prostate and testis cancers as well as identification of markers of male infertility In the case of prostate...

Ngày tải lên: 14/08/2014, 16:21

... vertebrates The human intermediate lobe is less pronounced than in other vertebrates consisting of only a thin layer of cells Because of the diminutive size of the human intermediate lobe, humans produce ... EMSA analysis of PITX2A, LHX3, and LHX4 binding of TAAT elements in Core R3 64 4.1 A schematic summary of findings 77 4.2 A hypothetical mechanism for regulation of the ... overlapping functions The presence of one functional allele of either results in the formation of a definitive Rathke’s pouch Pituitaries of mice with complete loss of both LHX3 and LHX4 proteins...

Ngày tải lên: 24/08/2014, 11:06

... FUNCTIONAL ANALYSIS OF HUMAN NIMA-RELATED KINASE (Nek8) LIU JUN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE ... Identification of hNek8…………………………………………… 54 3.1.1 Isolation and sequence verification of human Nek8………… 54 3.1.2 Bioinfomatics analysis of hNek8……………………………… 56 3.2 Characterization of hNek8……………………………………… ... TABLE OF CONTENTS ACKNOLEGEMENTS……………………………………… I TABLE OF CONTENTS…………………………………… II SUMMARY………………………………………………….VII LIST OF FIGURES………………………………………… IX LIST OF TABLES………… XI LIST OF ABBREVIATIONS………………………………XII...

Ngày tải lên: 11/09/2015, 10:03

... objectives of this thesis Published research in biodegradation of aromatic compounds and the current status of the state -of- the-art techniques in proteomics and the applications of proteomics ... cells in the biofilm Akinson (1981) had reported the use of biofilms for water treatment Since then, biofilm reactors have become a focus of interest in the field of bioremediation Lots of studies ... Utilization of Aromatic Pollutants 2.2.1 2.2.2 2.2.3 2.2.4 2.3 Proteomics Technologies in Bioremediation 2.3.1 2.3.2 2.4 Proteomics Technologies Proteomics and Bioremediation Proteomics Studies of P...

Ngày tải lên: 12/09/2015, 08:19