suppression of pro inflammatory cytokines via targeting of stat responsive genes

... magnitude of GABA-mediated currents [10], supporting the role of pro- inflammatory cytokine contribution to the generation of fever-induced seizures [9] Also, IL-1b prolongs the duration of electroencephalographic ... a significant elevation of HMGB1 in the serum of febrile seizure patients Moreover, serum levels of other pro- inflammatory cytokines, including IL-1b, IL-6, and the antiinflammatory cytokine IL-10 ... each child’s parents Cytokine measurement Levels of pro- inflammatory cytokines including HMGB1, IL-1b, IL-6, interferon (IFN)-b, TNF-a, and anti -inflammatory cytokine IL-10 were measured using...

Ngày tải lên: 19/06/2014, 22:20

... is not clear if pro- inflammatory cytokines affect expression of genes encoding other enzymes of the KP While human fibroblasts have previously been employed for studying the role of IDO1 in controlling ... Cite this article as: Asp et al.: Effects of pro- inflammatory cytokines on expression of kynurenine pathway enzymes in human dermal fibroblasts Journal of Inflammation 2011 8:25 Submit your next ... a pronounced and selective increase (almost 30-fold) in levels of transcripts encoding KYNU, suggesting a direct influence of TNF-a on expression of this gene Thus, it appears as if certain cytokines...

Ngày tải lên: 11/08/2014, 03:20

... is not clear if pro- inflammatory cytokines affect expression of genes encoding other enzymes of the KP While human fibroblasts have previously been employed for studying the role of IDO1 in controlling ... Cite this article as: Asp et al.: Effects of pro- inflammatory cytokines on expression of kynurenine pathway enzymes in human dermal fibroblasts Journal of Inflammation 2011 8:25 Submit your next ... a pronounced and selective increase (almost 30-fold) in levels of transcripts encoding KYNU, suggesting a direct influence of TNF-a on expression of this gene Thus, it appears as if certain cytokines...

Ngày tải lên: 11/08/2014, 06:23

... http://www.journal-inflammation.com/content/6/1/1 Effect of2 Figure POMx on the production of pro- inflammatory cytokines in PMACI-stimulated KU812 cells Effect of POMx on the production of pro- inflammatory cytokines in PMACI-stimulated ... recruitment of activated immune and inflammatory cells to the site of inflammatory lesion, thereby amplifying and sustaining the inflammatory condition [8] The pro- inflammatory cytokines produced ... expression of pro- inflammatory cytokines To determine whether the inhibition of IL-6 and IL-8 expression was mediated by inhibition of MAPK, we examined the effect of POMx on the activation of MAPKs...

Ngày tải lên: 11/08/2014, 08:22

... networks Expression pro les of auxin -responsive genes during reproductive development Expression pro ling can provide information about the functional diversification of different members of a gene family ... comprehensive expression pro ling of auxin -responsive genes in a large number of tissues ⁄ organs and developmental stages in this study To achieve gene expression pro ling of auxinresponsive genes identified ... and J P Khurana Transcript pro ling of auxin -responsive genes Fig Real-time PCR analysis of selected genes to validate their expression pro les during various stages of development The mRNA levels...

Ngày tải lên: 07/03/2014, 01:20

... to be an important cofactor with other pro- inflammatory cytokines such as IL-1, TNF-α and IL-17 in mediating cartilage/bone destruction [9,18,19] When these proinflammatory cytokines are overexpressed ... synovial hyperplasia and an infiltration of inflammatory cells Marked synovial hyperplasia with angiogenesis was also seen with bone erosions (arrows) and bone fractures, with evidence of synovial ... number of factors contribute to arthritic cartilage/bone destruction in RA, including the proliferation of synovial cells, the influx and interaction of inflammatory cells, and the maintenance of...

Ngày tải lên: 09/08/2014, 06:22

... response The inflammatory DCs are responsible for secretion of cytokines with a proinflammatory function like TNFa, IL-12p70, IL-23, and several other inflammatory mediators like nitric oxide, prostaglandins ... receptors provide negative signals that suppress effector T-cell responses [6] Finally, DCs are the source of secretion of very potent pro- inflammatory cytokines and chemokines The hall-mark cytokines ... stimulating cocktails were designed by series of combinations of pro- inflammatory cytokines and TLR agonists The cytokines TNFa, IFNg, IFNa IL-6 and IL-1b were from Peprotech (London, UK) and diluted in...

Ngày tải lên: 11/08/2014, 06:22

... Joint synovial cytokines and chemokines were thus measured to evaluate whether the mechanism of ERα-mediated immunesuppression is associated with changed levels of cytokines such as IL-1β Of 18 different ... of 12 Table 3: ERα-mediated suppression of arthritis is associated with decreased cytokine and chemokine levels IL-1β (pg/mg protein) IL-6 (pg/mg protein) IL-12p40 (pg/mg protein) KC (pg/mg protein) ... but not ERβ in suppression of inflammatory processes in experimental arthritis Materials and methods Mice and rats All the experiments were approved by the Animal Welfare Committee of Schering-Plough,...

Ngày tải lên: 12/08/2014, 14:21

... concentrations of cytokines in comparison to levels of F11R mRNA measured in the absence of cytokines Since the inhibition of the activity of NFB by parthenolide did not block the increase in the level of ... inhibitor of RNA synthesis, and F11R-siRNA, a specific inhibitor of the synthesis of F11R-mRNA Both of these inhibitors blocked the enhancement of expression of F11R-mRNA and of the synthesis of the ... involvement of the JAK /STAT signaling pathway in the induction of F11R mRNA and the de-novo expression of the F11R protein by IFNg As the analysis of F11R gene structure indicates the presence of two promoters...

Ngày tải lên: 18/06/2014, 19:20

... deposits and produce a variety of inflammatory mediators including proinflammatory cytokines such as IL-1β, IL-6, and tumor necrosis factor-α, as well as chemokines, reactive oxygen species, proteolytic ... Journal of Neuroinflammation 2006, 3:18 Background Deposition of the amyloid β-protein (Aβ) in brain is a prominent pathological feature of Alzheimer's disease (AD) and a number of related ... cultures of HCSM cells were incubated in the presence or absence of 25 μM Dutch mutant Aβ40 with daily administration of anti -inflammatory drug (1 μM final concentration) After six days the viability...

Ngày tải lên: 19/06/2014, 22:20

... been shown that reduction of pro- inflammatory mediators produced by microglia may attenuate the severity of neuronal damage [7] Therefore, inhibiting inflammatory cytokine production by activated ... number of major cellular responses that play critical roles in the pathogenesis of inflammatory responses and has been commonly used to model proinflammatory and neurotoxic activation of microglia ... production of LPS-induced pro- inflammatory cytokines IL-1b and TNF-a using ELISA As shown in Figure 2B and 2C, stimulation of BV-2 cells with LPS led to a significant increase in the levels of...

Ngày tải lên: 19/06/2014, 22:20

... proinflammatory gene promoters but this binding is stronger when induced by LPS+IFNg Lack of C/EBPb results in attenuated expression of proinflammatory genes and, again, this effect is more pronounced ... LPS and LPS+IFNg induce expression of the four pro- inflammatory genes studied but not affect mRNA levels of the anti -inflammatory genes IL-4 and TGFb1 Absence of C/EBPb results in significant decreases ... component of C/ EBPs DNA binding complexes during LPS- and LPS +IFNg-induced glial activation Next, we estimated the binding of C/EBPb to the promoters of four major pro- inflammatory genes: nitric...

Ngày tải lên: 19/06/2014, 22:20

... include marked proliferation of synoviocytes in the synovial lining layer, gross infiltration of lymphocytes, and migration of atypical lymphocytes with nuclear indentation into the synovial fluid ... diagnosed with primary OA of one or both knee joints We also studied the histopathological features of synovia of eight HTLV-I carrier patients, and determined the expression of Tax protein by immunohistochemistry ... layers, and inflammatory reaction was subtle or minimal (Table 2) In contrast, the two synovia of IgM-positive patients consisted, in part, of papillary projected synovium with synovial lining...

Ngày tải lên: 09/08/2014, 01:23

... contributes to the pathogenesis of CIA in these mutant mice by promoting DTH and by interfering with migration of CD11b+ cells into joint tissues A major difference in the pathogenesis of CIA between ... The pathogenesis of CIA is generally considered to depend on both humoral and cellular immunity against CII To see whether inhibition of CIA by AMD3100 acts via modulation of either of these, ... that, in the course of CIA pathogenesis, CXCL12 might stimulate or enhance production of certain cytokines and that this might be a pathway by which AMD3100 could exert its protective action To...

Ngày tải lên: 09/08/2014, 07:20

... microbial products and proinflammatory cytokines such as IL-1β and tumour necrosis factor alpha (TNF-α) [5,6] Some subsets of chemokines, such as the CXCR3 ligands, were discovered as proteins ... (Mr) of proteins was calculated with the Bruker deconvolution software In addition, the NH2-terminal sequence of chemokines was determined by Edman degradation on a capillary protein sequencer (Procise ... values of the reaction mixtures before the addition of GlyPro-pnitroanilide were subtracted from the obtained values to represent the real increase of OD400 values as a measurement of proteolytic...

Ngày tải lên: 09/08/2014, 08:22

... radiotherapy is capable of activating NFB, which subsequently stimulates increased production of pro- inflammatory Figure Protein expression of TNF in the jejunum following six weeks of fractionated ... Fellowship Professor Dorothy Keefe is the Cancer Council South Australia Professor of Cancer Medicine Author details School of Medicine, University of Adelaide, Adelaide, South Australia School of Medical ... the elevated levels of pro- inflammatory cytokines following five and six weeks of radiotherapy correlates with histological evidence of intestinal mucositis and peak expression of NFB [13] Together,...

Ngày tải lên: 09/08/2014, 08:22

... is a potent trigger of arthritis J Intern Med 2004, 255:344-350 Jiang W, Pisetsky DS: Mechanisms of Disease: the role of highmobility group protein in the pathogenesis of inflammatory arthritis ... infliximab therapy on the synovial expression of other important pro- inflammatory molecules [38,47] Infliximab treatment in another small cohort of patients with RA saw the number of TNF-synthesising cells ... positive feedback loop and induce production of several pro- inflammatory cytokines such as IL-6, IL-1β and TNF by macrophages and dendritic cells, thereby sustaining prolonged inflammation [16,36]...

Ngày tải lên: 09/08/2014, 10:23

... Results Effects of statins on C6 glioma cell proliferation and viability To examine the cytotoxic effects of mevastatin, fluvastatin, or simvastatin on C6 glioma cells, C6 glioma cell proliferation ... cytotoxic effects of mevastatin, fluvastatin, or simvastatin on U251MG cells, the survival of these cells was assessed in the presence of mevastatin (1-10 μM), fluvastatin (1-10 μM), or simvastatin (2.5-20 ... activation of the MEK/ERK and PI3K/Akt pathways [14,16], suggesting the mechanism of action of statins The treatment of C6 glioma cells with μM mevastatin, μM fluvastatin or 10 μM simvastatin for...

Ngày tải lên: 10/08/2014, 10:21

... of Washington as Assistant Professor of Medicine in the Division of Allergy and Infectious Diseases and rose to the rank of Professor of Medicine and Adjunct Professor of Pathology In March 2006, ... that Fas-mediated release of HMGB1 from mϕ contributes significantly to Fas-induced proinflammatory cytokine production by mϕ Fas induced production of pro- inflammatory cytokines is TLR4-dependent ... translocation of HMGB1 in mϕ Release of HMGB1 contributes to Fas-induced proinflammatory cytokine production by mϕ Both Fas and HMGB1 have previously been shown to induce pro- inflammatory cytokine production...

Ngày tải lên: 11/08/2014, 03:20