Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... plating of hESC-derived chondrogenic cells Fig 17 Chondrogenic differentiation capability of hESC-derived chondrogenic cells Fig 18 Analysis of pluripotency and lineage- restriction of hESC-derived chondrogenic ... explored for their potential as viable cell sources for cartilage tissue engineering (Chung et al., 2008) Human embryonic stem cells (hES...
Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps
... of human embryonic stem cells Stem cells 2005, 23:1228-1233 Moon SY, Park YB, Kim D-S, Oh SK, Kim D-W: Generation, culture, and differentiation of human embryonic stem cells for therapeutic applications ... Daley GQ: Therapeutic potential of embryonic stem cells Blood Rev 2005, 19:321-331 Menendez P, Wang L, Bhatia M: Genetic manipulation of human embr...
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... changed to DMEM/F12 (Invitrogen, 10 565-042) supplemented with Non-essential amino acids (Invitrogen, 11 114 0050), N2 (Invitrogen, 17 502-048), heparin (Sigma, H 314 9) and FGF-2 (ProSpec, CYT-557 _10 µg) ... harvested using collagenase and seeded onto CoStar non-adherent plates (Corning, 34 71) to form embryoid bodies using 20% Knockout Serum (Invitrogen, A1099202) in Knockout DME...
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... IPI0 022 0 327 Extracellular Matrix Ribosome-binding protein 66 022 IPI0 029 7646 138 923 IPI003049 62 129 297 IPI00013079 IPI00 328 113 IPI00 329 573, IPI009645 52 IPI00 022 200, IPI000 729 18, IPI0 022 0701 IPI00 022 418, ... Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, based on work by Hughes et al 20 09 † Murine protein...
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... matrices contains more Fn (by immunofluoresence comparison, section 3. 3.2), it would be expected that the 52 increased presence of Fn would aid in increasing integrin binding and hence cell binding This ... This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs...
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
... gastrulation in an anterior-posterior pattern Germ cells continue to maintain the expression of Oct -4 until the initiation of sexual differentiation [10] The under-expression of Oct -4 in ES cells ... their differentiation into trophoectoderm lineages [10], indicating the crucial role that Oct -4 plays in maintaining the pluripotent state of ES cells As Oct -4 is a transcription fa...
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
... and were transferred onto laminincoated plates and incubated in neural induction medium Under these conditions, hESCs were able to form extensions between embryoid bodies that were stained positive ... that maintain hESC pluripotency using dispase 3.7.1 Population doublings In section 3.4, 3 .5 and 3.6, all hESCs were passaged by enzymatic passaging using collagenase IV There is an...
n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
... collagen deposition: Fab’ to the gelatin binding domain of fibronectin inhibits both fibronectin and collagen organization in fibroblast extracellular matrix J Cell Biol 92:485-492 (1982) 52 Velling ... amount of a beneficial substance might have adverse effects Indeed, it has been reported that MEFCM contains BMP-antagonists, noggin and gremlin, and hESCs can be maintained in...
báo cáo hóa học:" Transplantation of vascular cells derived from human embryonic stem cells contributes to vascular regeneration after stroke in mice" docx
... transplanted vascular cells derived from human ES cells and the vascular density in the infarct area after the transplantation In the saline- and hMNCs-injected groups, the vascular density of host ... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional cu...
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... family, the miR-200 family have been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining ... instance, miR-373 induces the expression of E-cadherin and CSDC2 by targeting their promoter region and initiate their expression[ 73] Another me...
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... specification of midbrain dopamine neurons Radial glia cells are neuronal progenitors in vivo 10 11 14 1. 3 Long non- coding RNAs in biology 15 1. 3 .1 1.3.2 1. 3.2 .1 1.3.2.2 1. 3.2.3 Long non- coding RNAs in pluripotency ... pluripotency, and repressing differentiation simultaneously 18 1. 3.2 Long non- coding RNAs in neural developmen...
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... chr 13: 36 ,31 5,670 -36 ,31 7,9 83 chr4:1,179,572-1,181,6 03 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 AK124684 AK125262 AK12 539 2 AL 832 189 AY927 532 BC008027 BC 030 122 BC 031 955 ... location (NCBI36/hg18) AF242771 AK05 533 5 AK056826 (lncRNA_ES1) AK0911 13 AL117559 AL 833 138 BC02 630 0 (lncRNA_ES3) chr12: 63, 376, 238 - 63, 376,597 chr19: 43,...
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... was confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression ... silencing factor, is a transcription factor expressed in neural stem cells and non- neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds up...
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... established in the mouse model system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic ... B., Liu, M., and Shi, T (2011) Comparative analysis of human protein -coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq PLoS...