human embryonic stem cells from laboratory and clinical perspectives

... challenges Methods Cells Vero cells derived from African green monkey kidney (a kind gift from Professor E G Westaway, Sir Albert Sakzewski Virus Research Laboratory, Queensland, Australia) were ... kind gift from Professor E G Westaway, Sir Albert Sakzewski Virus Research Laboratory, Queensland, Australia), was propagated in Vero cells throughout this study Flavivirus infection of cells The ... preparation and AFM studies NML conceived of the study, and participated in its design and coordination Both authors read and approved the final manuscript Publish with Bio Med Central and every...

Ngày tải lên: 11/08/2014, 00:22

... the NTD hexamer from HIV-1, the high-resolution X-ray model of the modified CA hexamer from HIV-1, and the pseudoatomic models of the HIV-1 hexamer obtained from cryo-EM [30] and electron cryocrystallography ... The NTD–NTD interface is defined by contacts between residues in helices and from a NTD monomer and residues in helices and from the neighboring monomer These models are also validated by: (a) mutational ... MHR); and (c) NTD residues participate to some extent in intrahexamer and ⁄ or inter-hexamer interactions (Fig 1) 6100 This model remains to be validated and extended to higher resolution, and...

Ngày tải lên: 18/02/2014, 06:20

... Astier-Gin et al plus-strand RNA has been determined [10,11] and the involvement of the three stem loops of the 3¢X has been extensively studied both in vitro, in the replicon system and in vivo [7–9,12] ... minus-strand RNA in vitro and that deletion of the 3¢ 45 nt greatly impaired RNA synthesis [17] Oh et al [18] showed that RNA synthesis from the HCV minus-strand RNA required a minimum of 239 nt from ... RNA and that the secondary structures of the stem loops SL-A1, SL-B1, SL-C1 and SL-D1 were unmodified The 5¢-SL-E1 stem loop was unchanged in (–)IRES 239 whereas in (–)IRES 219, the base of the stem...

Ngày tải lên: 20/02/2014, 01:20

... the cervix, vulva, and vagina and effective against genital warts [13-15] Owing to the promising human clinical trials, this prophylactic quadrivalent HPV (types 6, 11, 16 and 18) L1 recombinant ... and complete protease inhibitor (Roche), and sonicated The sonicated suspension was centrifuged at 100,000 g at 10°C for 24 h Two distinct bands were observed on the CsCl gradient: the top band ... the adjacent cells and tissues triggered by immune recognition of a primary target, also called a bystander effect [23] Thus the levels of L1 would indeed be detected by the immune system but would...

Ngày tải lên: 20/06/2014, 01:20

... distribution patterns and phosphorylation status of PXR and RXRα in vitro and in vivo will further support these hypotheses and are currently underway in our laboratory Materials and methods Figure ... and μl of QuantumRNA™ 18S internal standard (Ambion, Austin, TX) at a primer:competimer ratio of 4:6 for CYP 3A2 and 2C11 and 3:7 for PXR and RXR reactions Gene-specific reaction conditions and ... 3-kinase (PI3K) and protein kinases A and C (PKA and PKC) which may contribute to the observed changes in CYP after administration of any of the vectors included in this study [34-36] Data from in vitro...

Ngày tải lên: 20/06/2014, 01:20

... show increased stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and non -human primates Blood 2002, 100:823-832 Sandrin V, Cosset FL: Intracellular ... Biochemical and functional analysis of Flu-VLPs Biochemical and functional analysis of Flu-VLPs A Incorporation of HA, NA and M2 proteins from H7N1 (A/Chicken/ FPV/Rostock/1934) or H5N1 (A/Thailand/KAN-1/04) ... contributions JS, DK and FLC conceived the study JS and FLC coordinated the research efforts and edited the manuscript BB, PJ, and MM contributed to parts of the experimental work MM, HDK, DK and VV provided...

Ngày tải lên: 20/06/2014, 01:20

... against the standard curve taking into consideration the dilution factor Results IFN-γ production No IFN-γ production was observed in infected and control cattle and buffaloes from to 16 weeks ... hepatica-resistant rats larvae of F hepatica were coated with antibody and host cells, including eosinophils, neutrophils, macrophages and mast cells, before they were destroyed within the peritoneal cavity ... 136 Elizabeth C Molina Fig IFN-γ profile in cattle (a) and buffaloes (b) infected with F gigantica and non-infected cattle and buffaloes were determined by ELISA The assay made use of mouse...

Ngày tải lên: 07/08/2014, 18:21

... synovial lining cells was assessed in at least five points in an area of more than 1.5 cm × 1.5 cm as follows: -, or cells thick; +/-, or cells thick; ++, 5–9 cells thick; +++, more than 10 cells thick ... control, SF and serum were also obtained from 58 HTLV-I-negative OA patients Comparison of HTLV-I carrier and non-carrier OA patients showed no obvious differences in age, sex, affected side and disease ... Nagasaki University School of Medicine, and a signed consent form was obtained from each subject ELISA and Western blotting of HTLV-I Anti-HTLV-I antibody in sera and SF was screened by enzyme-linked...

Ngày tải lên: 09/08/2014, 01:23

... contributions MIC and YFH contributed equally to this work MIC and YFH interpreted the data and drafted the manuscript MIC, MW, DC and JTC designed and performed the RNAi and JC virus experiments MZ and GJT ... baculovirus [4], E coli [14] and yeast [12] In addition, the VLP is able to package exogenous DNA and deliver it into human kidney [14], glioma [5] and colon adenocarcinoma [15] cells These findings ... risks for infection and are considered as a safe and efficient method for transfection of RNAi The JCV receptor is widely distributed among mammalian cells including human, monkey, and mouse JCV can...

Ngày tải lên: 10/08/2014, 05:21

... were chilled on ice and subjected to centrifugation at 13,000 rpm in a microcentrifuge The pellet and supernatant were designated as insoluble and soluble fractions respectively and the amount of ... the 5'- and 3' is shown at ThePP7 bottom, and the sequences2PP7itduplication ends of The sequence at the junction of the 2PP7 duplication is shown at bottom, and the sequences of the 5'- and 3' ... spaced main bands that likely correspond to circular pentamers and hexamers, and two less intense bands representing linear pentamers and hexamers (i.e "nicked" circles) This is in accordance with...

Ngày tải lên: 11/08/2014, 00:22

... of the curves showing the disappearance of the VLP band from gels, and of coat protein from the soluble fraction suggests that disassembly and denaturation/precipitation are roughly concomitant ... protein single-chain dimer from the bacteriophage T7 promoter, T7 transcription terminator, and the polylinker of pET3d and the kanamycin resistance determinant of and origin of replication of ... mutations in codons 14 and 15 [2] Insertion at Kpn I duplicates Gly14 and Thr15 We call this the 15/14 insertion-mode since amino acids 15 and 14 respectively flank the N- and C-termini of the...

Ngày tải lên: 11/08/2014, 00:23

... incidence and prevalence of human papillomavirus type 6, 11 and 16 infections in Finland J Gen Virol 2003, 84:2105-2109 zur Hausen H: Viruses in human cancers Science 1991, 254:1167-1173 Human papillomavirus ... specific and sensitive for the detection of rhesus macaque IgM, IgA, IgE, IgG4 and total IgG Low cross reactivity was seen with the anti-IgG1 and IgG2 antibodies to purified human IgG2 and IgG1 ... IgM, IgA, IgG1 and IgG4 antibody titers HPV L1-VLP specific IgM, IgA, IgG1 and IgG4 antibody titers Sera from rhesus macaques immunized at week 0, and 24 with µg each of HPV 6, 11, 16 and 18 L1-VLPs...

Ngày tải lên: 11/08/2014, 10:23

... eight patients with HIV and with either KS or KS and MCD, Boivin et al measured HHV-8 DNA levels of up to as high as 47,210/10E5 cells in PBMCs and 256/10 ul (25,600 cells/ ml) in plasma in the ... C, Ramazzotti E, Ensoli F, Andreoni M, Pezzotti P, Rezza G, Yarchoan R, Gallo RC, Ensoli B: Reactivation and persistence of human herpesvirus-8 infection in B cells and monocytes by Th-1 cytokines ... titles between August 2006 and March 2007 was noted Discussion The clinical presentation of fever, weight loss, generalized lymphadenopathy and enlargement of the liver and spleen in our patient...

Ngày tải lên: 11/08/2014, 17:21

... Health and Human Services/ National Institutes of Health/National Institute of Allergy and Infectious Diseases Challenge and Partnership Grant Numbers AI067188 and AI082119, and RC-0013-07 from ... http://www.virologyj.com/content/7/1/279 Page 10 of 19 Figure Binding profile of human serum IgM and IgG, and NP-specific mAbs on LASV VLP and recombinant nucleoprotein Human sera collected from household contacts of patient G676, ... RNA from Z3’HIS, Z3’HIS+GPC, Z3’HIS+NP, Z3’HIS+GPC+NP, and Z+GPC+NP (lanes 2, 4, 6, 8, and 10, respectively [V]), and μg of total RNA isolated from the corresponding transfected HEK-293T/17 cells...

Ngày tải lên: 12/08/2014, 01:22

... molecular weight marker; lane 1, bacterial lysates from cells without IPTG; lane 2, bacterial lysates from IPTG-induced cells in E coli; lane 3, pellet from bacterial lysates; lane 4, supernatant of ... protein VLPs from E coli TEM and immunoelectron microscopy of the nCap protein show that it can assemble into Cap-like particles with diameter ranging from 15 to 20 nm (Fig 4A and 4B), and an anti-PCV2 ... particles and present viral antigens in a more authentic conformation and biological function Therefore, they are easily recognized by the immune system and able to stimulate both B-cell and T-cell...

Ngày tải lên: 12/08/2014, 04:20

... HIV-1 VLP production by (A) insect cells and (B) mammalian cells Effects of DSB on HIV-1 VLP production by (A) insect cells and (B) mammalian cells (A), Sf9 cells infected with AcMNPV-Pr55Gag ... effect: assembly and release of VLP from Sf9 cells coexpressing Pr55Gag and VifC133S showed the same degree of DSB sensitivity as from Sf9 cells expressing Pr55Gag alone (Fig 6Bii, and Fig 6C) These ... precursor was expressed, or /and by viral proteins present in 5BD.1 cells and absent from Sf9 cells The following experiments were designed to address this issue, and to determine which factor(s)...

Ngày tải lên: 12/08/2014, 04:21

... pre-treatment viral production and clearance were high and that in vivo half-lives were a few hours for free HCV virions and 1–70 days for productively infected cells [10] Infected cell death ... to week 2, 4, 8, and 12 of treatment, week point time tend to be better than other time points for predicting SVR, with a PPV of 91% and a NPV of 89% and a very good sensitivity and specificity ... diversity and the opportunity for viral escape from host immune surveillance and antiviral therapy Ideally, interferon alpha serum levels should provide constant pressure on the virus and should...

Ngày tải lên: 13/08/2014, 13:20

... follows: HepG2 Cells Detection of ribozyme RB15 RNA in cells by one-step RT-PCR A human hepatoma cell line (HepG2 cells, × 105 cells) was seeded onto each well of a six-well plate until the cells reached ... HepG2 cells The expressed ribozyme RB15 in HepG2 cells HepG2 cells (5 × 105 cells/ well) were infected with rAAV-TTR-RB15 (1 × 1010 particles/well) (A) Total RNA was extracted from infected cells ... 80% confluence (~5 × 105 cells) The cells were infected with × 1010 particles of rAAV-TTR-RB15 or rAAV-TTR-RB15 mutant At days and after infection, RNA was extracted from cells using Trizol reagent...

Ngày tải lên: 14/08/2014, 19:22

... indicated primers Lane A: DNA from transfected 293T cells; lane B: no template control; lanes C and D: DNA and RNA from transduced 293T cells; lane E: DNA from mock transfected cells B) Translocation ... 293T cells; lane B: no template control; lanes C and D: RNA from transduced 293T cells with and without DNase treatment prior to reverse transcription; lanes E and F: RNA from mock transduced cells ... murine DCs and T cells Methods Parental plasmid and strategy used for packaging and vector construction Prototype vectors and packaging construct were developed from p∆00 (Fig 1A and 2A), a replication-competent...

Ngày tải lên: 14/08/2014, 19:22