Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 2
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 2
... intracellular Ca2+-release To investigate the participation of mitochondria- specific CD38 in mediating Ca2+ release from ryanodine-sensitive Ca2+ stores, an in vitro assay was set up using intact mitochondria ... activity and specific topology of CD38 and its role in Ca2+-release assay observed from the data, this suggest that mitochondrial CD38 plays a role in...
Ngày tải lên: 11/09/2015, 09:07
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 3
... that CD38 staining is mainly associated on the outer membrane of mitochondria It is interesting to note that instead of a uniform distribution of the CD38 staining around the outer mitochondrial ... Expressed in Mitochondia from Murine Brain 4.2.4 Determination of the enzymatic activities of brain mitochondrial CD38 4.2.4.1 Determination of ADP-ribosyl cyclase activi...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 4
... antibody-sc-7 049 A, B: intense CD38 immunostaining labeled the outer membrane of mitochondria (arrow), ER as well as plasmalemma (arrow head) Note the immunostained ER and mitochondria were in close ... bar: 1, 2µm 186 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure 4. 14 CD38 immunoreactivity in the mouse cerebellum, labeled wi...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 8
... Isolation and characterization of intact mitochondria from neonatal rat brain Brain Res Protocol 8: 176- 183 Nishina H, Inageda K, Takahashi K, Hoshino S, Ikeda K and Katada T (1994) Cell surface antigen ... organelles capable of generating and conveying electrical and calcium signals Cell 89 : 1145–1153, 1997 Ikehata F, Satoh J, Nata K, Tohgo A, Nakazawa T, Kato I, Kobayashi S, Akiy...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 5
... immunostaing is restricted to surface of mitochondria (arrow) Scale bar: 0 .5 m 189 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain Gd Figure 4.17 CD38 immunoreactivity in ... of staining with CD38 involving the surface of the outer mitochondrial membrane facing the cytosolic side In contrast to the immunogold labeling of CD38 on the o...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria
... mobilization agents such as ix cADPR, ADPR and NAADP and thus may have a critical role in intracellular Ca2+ signalling x ABBREVIATIONS ADPR adenosine diphosphate ribose ATRA all-trans-retinoic acid BSA ... NUDT9-H domain (Kuhn et al., 2004; Perraud et al., 2005) These data revealed that ADPR and NAD+ act as intracellular messengers and may play an important role in Ca2+ influx by a...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 6
... the surface of mitochondria 1 96 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure 4.20 Backscatter electron micrographs of mitochondrial fractions, labeled ... varying size of mitochondria with dense cristae with varying shapes of round, tubular to spherical (Figure 4.20B) 195 Chapter Characterization of CD38 Expressed in Mito...
Ngày tải lên: 11/09/2015, 09:09
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 7
... important to examine its precise localization on the organelle Mitochondria are compartmentalized into matrix, inner mitochondria membrane, intermembrane space and outer mitochondrial membrane, as discussed ... tend to argue against any artifactual rearrangement of the mitochondrial membranes during experimental manipulation All of the above considerations strongly suggest that...
Ngày tải lên: 11/09/2015, 09:09
Báo cáo khoa học: Subcellular compartmentalization of FADD as a new level of regulation in death receptor signaling pdf
... involving active caspase-8 Discussion FADD is an essential adaptor protein in the CD95mediated apoptotic signaling cascade that couples activated receptors with the activation of initiator caspase-8 ... triggering induces membrane proximal signals to induce nuclear export of FADD that are independent of CD95 internalization and ‘classic’ apoptotic signaling events, such as D...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo y học: " HIV infection of non-dividing cells: a divisive problem" ppt
... protein and HIV- 1 IN appear to behave in a remarkably similar way [25,140] Adenoviral pVII and HIV- 1 Rev and IN are all small, basic nucleic acids-binding proteins and may share similar nuclear import ... results cannot be accurately measured Alternatively, a difference in the rate rather than absolute amount of HIV- 1 DNA nuclear import may not be detectable in spreading assays du...
Ngày tải lên: 13/08/2014, 09:20
Heregulin enhanced tyrosine phosphorylation in breast cancer cells role of PP2A in HER 2 eu oncogenic signalling
... 44 2. 1 2. 1.1 2. 1 .2 2.1.3 2. 1.4 Materials Ligand and inhibitors Antibodies Cell-lines Fresh frozen clinical specimens 44 44 44 45 45 2. 2 2. 2.1 2. 2 .2 2 .2. 3 2. 2.4 2. 2.5 2. 2.6 2. 2.7 2. 2.8 2. 2.9 2. 2.10 ... REGULATION OF PP2A BY HER- 2/ neu 77 4.1 77 Overview 4 .2 4 .2. 1 4 .2. 2 4 .2. 3 4 .2. 4 Results 80 Inhibition of HER- 2/ neu signalling using AG...
Ngày tải lên: 11/09/2015, 10:06
A study to investigate the involvement of nadph oxidase 5 (NOX5) in resveratrol (RSV) induced reactive oxygen species (ROS) production in u937 cells
... esophageal cells resulting in esophageal adenocarcinoma (EA) Acid reflux damage is a major factor contributing to the transformation of EA NOX5-S was found to be the major NOX5 isoform present in ... is able to activate/ modulate signal transduction pathways such as the MAP kinase signaling pathways, JAK/STAT signaling pathways, PI3K/Akt pathway and cAMP/cGMP signaling p...
Ngày tải lên: 26/09/2015, 10:44
Statnamic testing of piles in clay 2
... 10.6 26 9.4 0 .28 3h -23 /8/04 22 B3 /22 /MLT MLT 28 0 4. 82 72. 61 9.5 27 0.5 0 .27 25 ,26 /8/04 23 B3 /23 /MLT MLT 28 0 4. 82 72. 61 9 .2 270.8 0 .27 27 / 12/ 8/04 Bed B4/1/CRP-0.01 CRP 28 0 0.01 3. 72- 3.6 56.04 13.3 26 6.7 ... 1.4 2. 5 2. 28 2. 28 10h -2/ 12/ 03 Date of test rate load pulse (kPa) mm/s (kN) (ms) 28 0 0.01 CRP 28 0 100 125 4.5 2. 2 2. 5 2. 53 2. 34 4...
Ngày tải lên: 07/11/2012, 10:44
Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot
... o⁄n Insulin receptor IRS1 PKB Glucose transport 1. 1 1. 8 0.6–0.7 0.9 1. 1 0 .1 0 .2 1. 1 1. 8 0.6–0.7 0.3–0.4 0. 02 0.03 1. 1 1. 8 1. 8 2. 0 0.6–0.7 0 .1 0 .2 1. 1 1. 8 1. 8 2. 0 0.6–0.7 0 .1 0 .2 1. 1 1. 8 1. 8 2. 0 ... 0.6–0.7 0 .1 0 .2 1. 1 1. 8 1. 8 2. 0 0.6–0.7 0 .1 0 .2 FEBS Journal 27 2 (20 05) 14 1 15 1 ª 20 04 FEBS 14 3 Insulin resistance in human adi...
Ngày tải lên: 07/03/2014, 16:20