Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain AA B B A B M Figure 4.12 CD38 immunoreactivity in the CD38 KO mouse cerebellum, labeled with goat polyclonal CD38 antibody-sc-7049. No immunoreactivity was observed in the granular layer. The insets (A & B) are enlargement of the encircled area of the granular layer. M-Mossy fiber terminals. Scale bar: 2µm. A & B, 1µm. 185 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure 4.13 CD38 immunoreactivity in the mouse cerebellum, labeled with goat polyclonal CD38 antibody-sc-7049. Immunoreactivity was present in the perikarya in association with the granular cell membrane viewed at low magnification. Upper panel: intense immunoreactivity observed in the granule cells, identified by its electron dense nuclei. Immunoreactivity was most intense with mitochondria. Varying degree in CD38 immunostaing of mitochondria observed in different compartment. Lower panel: immunopositive mitochondria located in Basket cell. The enlargements of the encircled area of the basketcell are shown in the following page. Blood vessel was heavily stained with CD38 antibody (arrow). Scale bar: 1, 2µm. 186 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure 4.14 CD38 immunoreactivity in the mouse cerebellum, labeled with goat polyclonal CD38 antibody-sc-7049. A, B: intense CD38 immunostaining labeled the outer membrane of mitochondria (arrow), ER as well as plasmalemma (arrow head). Note the immunostained ER and mitochondria were in close proximity with each other (B). Immunopostive mitochondria in close proximity with plasma membrane (A) Scale bar: A, B, 0.5µm. 187 . area of the granular layer. M-Mossy fiber terminals. Scale bar: 2µm. A & B, 1µm. A B A B M B A Chapter 4 Characterization of CD38 Expressed in Mitochondia from Murine Brain. immunoreactivity in the mouse cerebellum, labeled with goat polyclonal CD38 antibody-sc-7 049 . A, B: intense CD38 immunostaining labeled the outer membrane of mitochondria (arrow), ER as well as plasmalemma. immunopositive mitochondria located in Basket cell. The enlargements of the encircled area of the basketcell are shown in the following page. Blood vessel was heavily stained with CD38 antibody (arrow).