Claudin 1 is the direct target of RUNX3 in gastric epithelial cells

... Proteins in Mouse 71 Gastric Epithelial Cells 4 .1. 2 TJ Proteins and TGF-β Pathway 73 4 .1. 3 RUNX3 and claudin- 1 in TGF- β Pathway 74 4 .1. 4 claudin- 1 Expression in Mouse Gastric Epithelial Cells ... analysis of claudin- 1 expression 77 upon induction by RUNX3 in SNU16 cell line 4.5 Immunodetection of claudin- 1 in mouse gastric epithelial 7...

Ngày tải lên: 11/09/2015, 09:00

... oligo cDNA probe and a sense cDNA probe (complementary to the antisense) for mouse Slc1 2a2 mRNA were designed as follows; Slc1 2a2 antisense, 5¢-ATCTTCACAAGAAAAAT CACCTGGTACCAAGGATGT; Slc1 2a2 sense, ... Regulation of Clcn5 promoter by Six1 and Six4 (C) Trans-activation of Slc1 2a2 promoter by Six1 and Six4 (D) Transactivation of myogenin promoter by Six1 and...

Ngày tải lên: 07/03/2014, 17:20

... staining of STC-1 in tumor tissues of nude mice STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in tumor tissues of nude mice PCNA was detected in the ... Signaling Technology, USA) at 1: 1000, and the anti-btubulin rat monoclonal antibody (Beyotime, China) at 1:1000 VEGF Assay VEGF content in tumor culture supernatants...

Ngày tải lên: 10/08/2014, 05:21

... Nanyang Technological University, Singapore (Silver Best Poster Award) vi THE MECHANISTIC STUDIES OF THE ANTICANCER POTENTIAL OF ARTESUNATE IN HUMAN CANCER CELLS Table of Contents DECLARATION ... ART induced autophagy in human cervical cancer HeLa cells evidenced by the increase of autophagic flux In the search of the mechanisms for ART-induced...

Ngày tải lên: 09/09/2015, 08:16

... the importance of PT in the mitochondrial apoptosis, because in the absence of the hallmarks of PT, including the loss of the inner membrane potential and the rupture of the outer membrane, the ... hypoxia), and they found that the type, strength and kinetics of the target gene profiles depend on the levels of p53 in the cell, the type of in...

Ngày tải lên: 14/09/2015, 14:11

... biology of gastric epithelial cells due the loss of RUNX3 and how they contribute to tumorigenicity are not fully understood To investigate this, the Runx3+ /+.p53-/- and Runx3- /-.p53-/- gastric epithelial ... lines 121 Figure 6.1 A model summarising the role of Runx3 in protecting gastric epithelial cells against EMT -induced cellular plasticity and tum...

Ngày tải lên: 09/09/2015, 18:57

... GCTCAGTGGTGGA-3 and 5-CTGAGGGAAGCAAG AATGGA-3, OXI1 (At3g25250): 5-GACGAGATTATC AGATTTTACGC-3 and 5-AACTGGTGAAGCGGAAG AGAC-3, PTI1-4 (At2g47060): 5-CCCCAAAGAAAATG AGTTGCT-3 and 5-GCATCATTTCCTGGAGGAAAG-3 ... Journal 278 (2 011 ) 11 26 11 36 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 11 31 PTI1-4, a common target of OXI1 and MAPKs C Forzani et al AGC2-3) were also...

Ngày tải lên: 14/02/2014, 19:20

... pcDNA3.1-GST -Nur77 plasmid pSilencer-shNur77 was prepared by overlapping strategy with primers 5¢-gacGGATCCgcagtccagccatgctccttt caagagaaggagcatg-3¢ (with BamHI), 5¢-cggAAGCTTtATC GATccaaaaaacagtccagccatgctccttctcttg-3¢ ... 5¢-GACTCGCAGACAATGATGG TC-3¢ and 5¢-GCAAACTCATCATGGGCACC-3¢ The results were normalized with b-actin, for which the primers were 5¢-ATGGTGGGAATGGGTCAGAAG-3¢ and 5¢-CA CG...

Ngày tải lên: 23/03/2014, 07:20

... Business Value of Information Technology in the Internet Economy by Fang Yin, B.A Dissertation Presented to the Faculty of the Graduate School of The University of Texas at Austin in Partial ... Yin, Ph.D The University of Texas at Austin, 2002 Supervisors: Andrew B Whinston & Anitesh Barua This dissertation consists of three essays that addre...

Ngày tải lên: 18/02/2014, 11:20

... model constructed previously [46] (B) Snapshots of c conformation during the forced molecular dynamics calculated with the torque of 56 pNÆnm The secondary structure of c is shown for the time of ... portion of c at the level of cK18–cK21 and cD233–cS236 residues The torque was created by external forces acting on the two groups of four carbon atoms each T...

Ngày tải lên: 19/02/2014, 16:20

... target and partners of bFGF (Eur J Biochem 269) 507 Fig E€ect of bFGF in the presence of sodium chlorate on FSHstimulated estradiol synthesis in immature rat Sertoli cells Sertoli cells were incubated ... chlorate, in inducing structural alteration of HS chains, prevents the bFGF binding Our results indicate that the presence of sodium chlorate abolishes...

Ngày tải lên: 21/02/2014, 03:20

... motifs that are characteristic of RNA-binding proteins, and an alanine-rich carboxy-terminal sequence that could be involved in protein–protein interactions Interestingly, this alanine-rich sequence, ... Gld2 Rbm9 interaction (Fig 2B) However, the RRM-containing central domain of hRbm9 (amino acids 48–269) and amino acids 269–350 of hRbm9 are not able to mediate the binding These...

Ngày tải lên: 07/03/2014, 05:20

... 2012 International Monetary Fund WP/12/243 IMF Working Paper Western Hemisphere Department To Cut or Not to Cut? That is the (Central Bank’s) Question In Search of the Neutral Interest Rate in Latin ... paper) interest rate, the neutral nominal interest rate, stands for the rate of inflation, is the inflation target of the ce...

Ngày tải lên: 15/03/2014, 14:20

... map of the world In this map, the contours of Europe and Asia and Northern Africa are pretty well worked out But, some coastlines and interiors lack detail Off to the west, there is some sense of ... “art theorists… define the criterion of what the [aesthetic] experience is expected to be; scientists… provide a test of whether this criterion is fulfilled.”2 O...

Ngày tải lên: 23/03/2014, 13:20