Roles of tuberous sclerosis complex proteins in autophagy and cell death

... cullin3 Cylindromatosis cytochrome c death- associated protein-1 death- associated protein kinase DEP domain containing mTOR-interacting protein direct inhibitor of apoptosis-binding protein with ... Therefore, the main objective of this study is to investigate the roles of TSC proteins in cell death by focusing on the involvement of autophagy and JNK signaling pathway...

Ngày tải lên: 10/09/2015, 09:30

... Effects of inhibitor treatment (wortmannin and U0126) on insulin ⁄ IGF-1-induced phosphorylation of PI3K ⁄ Akt ⁄ mTOR-dependent signaling in C2C12 myotubes Fig S2 Complex formation of TSC1 and TSC2 ... versus control group (P < 0.05) Phosphorylation of Akt and S6K1 was induced within 10 min, and was maintained for at least 60 Altered distributions of TSC1 an...

Ngày tải lên: 15/03/2014, 11:20

... 3 .1 Mia1p in assembly and maintenance of persisent iMTOCs at nuclear envelope 44 3 .1. 1 Lack of microtubule binding protein Mia1p results in fewer interphase Microtubules 44 3 .1. 1 .1 Mia1p is a microtubule ... cytoskeleton 18 18 1. 2.4 .1. 1 Architecture and dynamics of interphase microtubule cytoskeleton 18 1. 2.4 .1. 2 Functions of interphase microt...

Ngày tải lên: 12/09/2015, 08:19

... center and plus-ends facing toward the cell tips Model of maintaining ordered microtubules (from Sawin and Tran, 20 06) is shown in the following cartoon 16 Model of interphase microtubules bundling ... critical role in maintaining cell morphology by binding to the plus ends of growing interphase microtubules in a Tip1p and Tea2p-dependent manner (Chang and Peter, 20 03)...

Ngày tải lên: 12/09/2015, 08:19

... 40μl TE buffer containing RNAse and incubated at 37 oC for 20min DNA was stored at -20oC 2 .3 Construction of knock out mutants and epitope tagging of genes 2 .3. 1 Construction of knock out mutants ... taxol-stabilized bovine brain 3X microtubules (50μg/ml) in the same buffer, followed by washing and immunoblotting withTAT1 anti-tubulin antibodies 2.2.5 Extraction of S .pom...

Ngày tải lên: 12/09/2015, 08:19

... kinetochore in metaphase (~1% of cells) and to the spindle midzone during anaphase (~5% of cells) in control cells Ark1p-GFP defined the spindle midzone in 43 % of Mia1p- overexpressing cells, and ... panel) and cells overexpressing Mia1p (right panel) coexpressing the NE marker protein, Uch2p-GFP, and the SPB protein, Sad1p-DSRed 84 3.2.3 Spindles in Mia1p- ov...

Ngày tải lên: 12/09/2015, 08:19

... interphase microtubule arrays 4 .5. 3 Lack of Mia1p and Alp14p result in failure of iMTOCs establishment Another striking aspect of Mia1p function has been illuminated by lack of large interphase MTOCs in ... that S pombe cells overexpressing the TACC- related protein, Mia1p, were capable of organizing spindle-pole like structures While formation of intranucl...

Ngày tải lên: 12/09/2015, 08:19

... spindle assembly checkpoint in fission yeast Nat Cell Biol 5, 764 - 766 ; author reply 766 68 Sato, M., and Toda, T (2007) Alp7 /TACC is a crucial target in Ran-GTPasedependent spindle formation in ... Isolation and characterization of AINT: a novel ARNT interacting protein expressed during murine embryonic development Mech Dev 97, 13- 26 66 Samejima, I., Lourenco, P.C., Snait...

Ngày tải lên: 12/09/2015, 08:19

... this article as: Rai et al.: An unusual case of congenital melanocytic nevus presenting as neurocutaneous melanoma coexisting with Tuberous Sclerosis complex: A case report Journal of Medical Case ... Provost N: A study of large congenital melanocytic nevi and associated malignant melanoma: review of cases in the New York University registry...

Ngày tải lên: 10/08/2014, 23:21

... role of AP-2a and AP-2e in chondrogenesis Overview of the differentiation stages during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of an ... analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target...

Ngày tải lên: 16/02/2014, 09:20

... both a novel C-terminal actin- binding submodule (CABS) containing a novel actin monomer binding verprolin homology C-terminal (VH2-C) domain and a second submodule comprising the previously characterized ... actinbinding domain (this actin- binding domain has not yet been mapped) [23] We name the actin- binding domain that we have identified VH2-C and VH2-...

Ngày tải lên: 18/02/2014, 16:20

... probable role of auxin-responsive genes in reproductive development and abiotic stress signaling in rice Transcript profiling of auxin-responsive genes study from our laboratory, the rice coleoptile ... 2009 FEBS M Jain and J P Khurana Transcript profiling of auxin-responsive genes Fig Expression profiles of auxin-responsive genes in various...

Ngày tải lên: 07/03/2014, 01:20

... How the unique features of the E focardii c-tubulins enhance microtubule nucleation by the c-TuRC complex at low temperature, and what these changes imply regarding the mechanism of nucleation? ... that c-T2, but not cT1, participates in the assembly of the mitotic spindle of E focardii and that both isotypes are involved in the nucleation of ot...

Ngày tải lên: 07/03/2014, 04:20

... damaged or inappropriate bases in DNA is base excision DNA repair (BER) [8–10] The initial step of BER involves enzymatic activities that process the N-glycosylic bonds linking the target bases and ... structurally distinct abasic sites, known as oxidized abasic sites Oxidized abasic sites include lesions at DNA strand breaks, such as 3¢-phosphoglycolate esters...

Ngày tải lên: 16/03/2014, 13:20

... overview of the regulation of pyruvate dehydrogenase complex activity in autotrophic plant cells Distinct regulatory mechanisms control the activity of mtPDC in the light and plPDC in the dark ... parallels that of fatty acid biosynthesis Thus, a unique mechanism for regulating activity of plPDC activity has evolved based Ó FEBS 2003 Regulation of PDC...

Ngày tải lên: 17/03/2014, 09:20