Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 1
Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 1
... 1. 5 Types of stem cells 14 1. 5 .1 Embryonic stem cells (ESCs) 15 1. 5.2 Induced pluripotent stem (iPS) cells 15 1. 5.3 Adult stem cells 16 1. 5.4 Heterologous stem cells 17 1. 6 MSCs in ... 1. 10.3 Iron Oxide Particles 24 1. 11 Cellular MRI 24 1. 11. 1 Cellular Imaging with Iron Oxide Particles 25 1. 11. 2 Mechanisms of Cellular Uptake 28 1. 12 Cell migration 30 1. 12 .1 Stem cell ... in cartilage repair 17 1. 7 Monitoring of Cell Therapy 18 1. 8 Histological Methods 19 1. 9 Imaging Modalities 19 1. 10 Contrast agents 22 1. 10 .1 T1 Contrast Agents (Table 1- 7) 23 1. 10.2...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 10
... of stem cells is a promising method for cartilage repair, in this chapter I confirmed that engraftment of the MSCs in injured cartilage is an active migration and homing process and injured cartilage ... microfracture (265, 266), or introducing new cell sources like debrided waste chondrocytes, Bone Marrow derived Mesenchymal Stem Cells (BM MSCs), or any combination of these cells (29, 214, 215, 267, ... exogenous stem cells (endogenous from the bone marrow and exogenous by multiple intra -articular injection of the expanded autologous stem cells) As I showed, in the previous chapter, that injection...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 2
... determined using 0.3% oil red O stain for 15 minutes at room temperature to stain intracellular lipids, and counterstained with hematoxylin Osteogenic differentiation was evaluated using Alizarin ... staining of the harvested tissues (197) 55 Table 3-1 Histological grading scale for cartilage repair (Adapted from (51)) Category Points Cell morphology Hyaline cartilage Mostly hyaline cartilage ... concentration in the culture media would increase the intracellular iron content of MSCs 61 Average iron content per cell (pg /cell) 20 0 150 100 50 Average iron content per cell (pg /cell) 0µg/ ml 25 µg/...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 3
... Oil red O staining demonstrated that unlabeled and labeled cells exhibited no difference in developing fat vacuoles (Figure 3. 9) 64 Figure 3- 9 Oil red O staining Oil red O staining of the ... (F) 3. 4.6.2 Osteogenic differentiation Alizarin red staining showed that MSCs labeled with SPIO did not affect osteogenic differentiation capacity of cells (figure 3. 10) Figure 3- 10 Alizarin ... (F) 65 3. 4.6 .3 Chondrogenic differentiation Immunohistochemical staining after high density pellet culturing showed that the distribution of collagen type II in extracellular matrix of cells was...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 4
... labeled cells in the knee joint) MSCs were distributed in different places of the knee (figure 3. 14) The labeled cells migrated to different locations over time including cartilage defect, injection ... MSCs in the mini-pig knee joint The labeled cells migrated to the para-patellar fat tissue (A) and surgical scar in the skin (B) 3 .4. 8 Postmortem analysis Histological grading scores of the repaired ... the injected cells were in defect site, para-patellar fat and injection site of the knee joint (figure 3.15 and 3.16) 71 Figure 3-15 MR imaging of the mini-pig's femoral condyle MR imaging...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 6
... confirming the report of Jing et al (2 06, 207) In addition, our results showed that we could detect labeled cells in vivo up to weeks, thus the SPIO labeling method of the cells can be a promising ... labeling concentration of MSCs can reduce the adverse effects of the iron particles Our results showed, after optimized labeling, that visualization of injected MSCs is indeed feasible in vivo Cells ... localization into different site of the inflammation was observed in the knee joint over time A majority of the cells moved to the surgery sites such as surgery scar, para-patellar fat and injection...
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Bone marrow derived mesenchymal stem cell (BM MSC) application in articular cartilage repair 7
... articular cartilage provides only a limited capacity of self -repair Cell based therapy is one promising approach in the treatment of damaged cartilage Bone marrow (BM) derived mesenchymal stem cell (MSC) ... Leukemia inhibitory factor Neurotrophin Chemokine (C-X-C motif) ligand 10 Colony stimulating factor (gran-macrophage) Colony stimulating factor (granulocyte) Integrin binding bone sialoprotein 97 ... transforming growth factor beta-3 (TGFβ3), bone morphogenetic protein (BMP4), vitronectin (VTN) Conclusion: As I showed in the previous chapter, injection of stem cells in the knee is a promising...
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Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates
... Types of cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal ... Casey K Chan Effects of mechanical stimulation in osteogenic and chondrogenic differentiation of bone marrow- derived mesenchymal stem cells on nanofibrous scaffolds Journal of Bioactive and Compactable ... mineralized NFS enhanced early cell capture of osteoblasts within 30 minutes The osteogenic differentiation potential of bone marrow derived mesenchymal stem cells (BM- MSCs) was achieved by manipulating...
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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
... Dou KF, Yang GS, Willerson JT, Geng YJ: Combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells increases benefits in infarcted swine hearts Arterioscler Thromb Vasc Biol ... neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation Journal of Biomedical Science 2010 17:75 Submit ... decreased muscle cell apoptosis and improved blood reperfusion of ischemic muscle following combined therapy of highdose simvastatin and bone marrow-derived MSCs Bone marrow-derived MSCs have been...
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Báo cáo sinh học: "Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction" ppt
... as: Chen et al.: Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction Journal of Translational ... adipose-derived mesenchymal stem cells (ADMSCs) After culturing for 14 days, majority of isolated adipose-derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = 3) Note ... tissue mesenchymal stem cells after transplantation into mice with liver injury Stem Cells 2008, 26:2705-2712 Sun CK, Zhang XY, Zimmermann A, Davis G, Wheatley AM: Effect of ischemia-reperfusion injury...
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Báo cáo hóa học: "Early combined treatment with sildenafil and adipose-derived mesenchymal stem cells preserves heart function in rat dilated cardiomyopathy" doc
... as: Lin et al.: Early combined treatment with sildenafil and adipose-derived mesenchymal stem cells preserves heart function in rat dilated cardiomyopathy Journal of Translational Medicine 2010 ... of heart function in DCM rats Accordingly, our finding strengthens the finding of previous study [20] Another important finding in the current study is that combined therapy with ADMSCs and sildenafil ... significantly lower in group than in groups and 5, and also notably lower in group than in Page 10 of 16 group This finding indicates that early combined treatment with sildenafil and ADMSCs is better...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... stimulation by distinct inflammatory injuries [3,44] EPC have been shown to display a higher proliferative potential [45] and may migrate to regions of the circulatory system with injured endothelia, including ... hypothesis whether the homing of intravenously administered bone marrow- derived EPC occurred in damaged lung tissue after the setting of severe tissue injury, as previously shown in part in an abstract ... http://respiratory-research.com/content/8/1/50 Figure Rat bone marrow- derived EPC in culture Rat bone marrow- derived EPC in culture A highly purified population of EPC was isolated from hindlimb bone marrow...
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Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx
... recognized by the SH3 antibody, and express prolyl-4-hydroxylase Prolyl-4-hydroxylase was shown to be expressed by synoviocytes [18,24] but to be absent on BM-MSC using FACS analysis [18] In ... differentially expressed in bone marrow-derived or synovium-derived cells Genes whose mRNA expression is differentially expressed in bone marrow-derived or synovium-derived cells Gene Upregulated in bone ... in a mixed lymphocyte reaction (MLR) and to display indoleamine 2,3-dioxygenase (IDO) activity Importantly, using macroarray technology we provide evidence that the transcriptional profiles could...
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Từ khóa: osteogenic differentiation strategies for adipose derived mesenchymal stem cellscell mesenchymal stem cell differentiation in vitrogeneration of functional islets from human umbilical cord and placenta derived mesenchymal stem cells3 placental tissue and the umbilical cord is an important source of fetal mesenchymal stem celldissecting mesenchymal stem cell movement migration assays for tracing and deducing cell migrationhematopoietic stem cell niche bone marrowbone marrow and peripheral blood stem cell transplantation pdfcollection and processing of peripheral blood stem cells and bone marrow pdfisolation differentiation and characterisation of skeletal stem cells from human bone marrow in vitro and in vivofunctional identification of neural stem cell derived oligodendrocytesmethods to analyze the homing efficiency and spatial distribution of hematopoietic stem and progenitor cells and their relationship to the bone marrow endosteum and vascular endotheliummouse bone marrow mononuclear cells mbm mnc human cd34 progenitors cells hcd34 mouse ms 5 bm strsources—bone marrow bm versus peripheral blood pbsc —for hsct in mdsrat mouse mesenchymal cells from bone marrowapplications of microfluidics in stem cell biologyNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíChuong 2 nhận dạng rui roKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015MÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ