Biochemical characterization of the bi lobe complex in trypanosoma brucei

... Biochemical characterization of the bi- lobe complex in Trypanosoma brucei LADAN GHEIRATMAND A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES ... Structural complexity of the bi- lobe 106 4.4 Characterization of bi- lobe associated proteins reveals extensive connection of bi- lobe to other cellular struct...

Ngày tải lên: 10/09/2015, 09:09

... by semiquantitative analysis of transcripts by Northern blotting and also by quantification of the enzyme protein by Western blotting Expression of Pf MDH, Pf LDH and Pf MQO in control and drug ... and purity of RNA the gels were stained with ethidium bromide and visualized on a UV transluminator Equal intensity of ribosomal RNA bands in all of the lanes ind...

Ngày tải lên: 16/03/2014, 18:20

... that the individual domains of the megasynthase may remain folded despite the lack of the KS dimer Minimal polyketide synthase domains in LovB Characterization of the standalone MAT protein The LovB ... in the yield of the soluble protein Pantetheinylation of the ACP in ACPCON didomain was verified using MALDI-TOF of a tryptic fragment of...

Ngày tải lên: 30/03/2014, 08:20

... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein interaction domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context...

Ngày tải lên: 16/02/2014, 09:20

... The Authors Journal compilation ê 2006 FEBS M H Dicko et al Characterization of sorghum peroxidase Table Purication of the major sorghum peroxidase Step apparent mass of 32 kDa (Fig 1B) Together ... health Therefore, knowledge of biochemical properties of the major peroxidase can help on sorghum processing In this study, we have puried and characterized th...

Ngày tải lên: 16/03/2014, 13:20

... particular, the biochemical aspects of the 3-HK dependent synthesis of XA in Anopheles gambiae, the most efficient vector of the most deadly malaria parasite P falciparum, remain elusive We report here the ... than that of the A gambiae enzyme ( lmolÆ min)1Æmg)1) The possible physiological ⁄ functional significance and the implications of these differences be...

Ngày tải lên: 16/03/2014, 23:20

... contained the EVH2 domain (Fig 1A) EVL( 1–221) and EVL( 222– A B C We first tested the ssDNA-binding and dsDNA-binding activities of EVL( 1–221) and EVL( 222–418), because the DNA-binding activity of EVL ... The EVH2 domain is responsible for the DNA-binding activity of EVL Preparation of EVL( 1–221) and EVL( 222–418) To identify the functional domain responsible...

Ngày tải lên: 23/03/2014, 04:20

... degradation, the degradosome is also responsible for the processing of 5S ribosomal RNA and tRNAs, as well as the degradation of tmRNAs [4–8] The principal proteins comprising the degradosome include the ... degradosome complex in Vibrio angustum S14 have expanded on these results and shown that RhlB can interact with the CTH of V angustum S14 RNase E...

Ngày tải lên: 29/03/2014, 21:20

... guide for biochemical purification of other potassium channels Results Expression and biochemical characterization of native Kv2.1 Regional distribution in various rat tissues of Kv2.1 channel protein ... from recombinant cell lines Here we report the biochemical characterization of Kv2.1 protein complexes The biochemical profile of the Kv2.1 potassium...

Ngày tải lên: 30/03/2014, 20:20

... step and to elute the recombinant enzyme in the elution step were determined in preliminary experiments Fractions were analysed for epoxide hydrolase activity and protein content based on the ... probably corresponding to the histidine and tyrosine residues, respectively [6–8] Several compounds were tested for potential inhibitory effects on the hydrolysis of TSO...

Ngày tải lên: 31/03/2014, 08:20

... This is the first study to examine the binding of DBA, SBA, Isolectin B4, WGA, PNA, and UEA-I in the vomeronasal organ of the horse We found unique characteristics of lectin binding depending cell ... binding; ++, >66% binding Lectin Isolectin B4 WGA ＋ ＋＋ － ＋＋ － － － － － ＋＋ PNA ＋ － － － － UEA-Ⅰ ＋＋ － － － ＋ Histochemical Characterization of the Lec...

Ngày tải lên: 07/08/2014, 17:22

... 15.69% in the alkalinity induced library The relatively lower percentage of 24 nt reads indicates that more kinds of miRNAs are involved in the response of G max to alkalinity compared with other stress ... complete profile of miRNAs in Glycine max Keyword: deep sequencing, Glycine max, microRNAs, stresses associated, miRNA Background Terrestrial plants face...

Ngày tải lên: 11/08/2014, 11:21

... be involved in the induction of apoptosis, including the 7a and 7b proteins [18,46,47] To analyze whether the deletion in ORF 7b had any influence on its Amino acid variability in ORF 7b and ... overexpression of ORF 7a, ORF interferon induction the Influence on apoptosis and type I 7b, and ORF 7b with by Influence on apoptosis and type I interferon indu...

Ngày tải lên: 12/08/2014, 04:20

... apoptosis, including the 7a and 7b proteins [18,46,47] To analyze whether the deletion in ORF 7b had any influence on its Amino acid variability in ORF 7b and RT-PCR analysis of ORF 7b in clinical ... sequences in GenBank (except in an independent sequence of the Frankfurt strain) , and in none of SARSlike bat-CoV sequenced in the ORF 7b region...

Ngày tải lên: 12/08/2014, 04:20

... presentation of the bi-directional different The the The schematic presentation of the bi-directional promoter and the parts and directions of the promoter in different constructs , indicates the deleted ... described[17] Construction of recombinant plasmids expressing CAT gene under the control of different promoters Construction of the bi-dir...

Ngày tải lên: 12/08/2014, 04:21