The role of ORF3 protein in the molecular pathogenesis of porcine circovirus 2 infection

... play a role in determining the pathogenicity of the PCV2 infection (Ramamoorthy et al., 20 09) This thesis describes the role of ORF3 in the pathogenesis of the PCV2 virus infection at the systemic ... the role of ORF3 in the pathogenesis of the virus infection in SPF piglets 28 2. 2 Materials and methods 2. 2.1 Viruses and cell cult...

Ngày tải lên: 10/09/2015, 08:38

... as: Yi and Liu: Molecular characterization of porcine circovirus isolated from diseased pigs co-infected with porcine reproductive and respiratory syndrome virus Virology Journal 20 10 7 :28 6 ... inoculation of conventional pigs with porcine reproductive and respiratory syndrome virus and porcine circovirus J Virol 20 02, 76: 323 2- 3...

Ngày tải lên: 12/08/2014, 01:22

... K Identification of Genetic characterization of porcine circovirus- 2 field isolates from PMWS pigs 39 27 28 29 30 31 32 33 34 35 36 porcine circoviruses with genetic variation from lymph nodes ... ORF2 of PCV -2 corresponding to PCV-1s showed a homology of about 63 %, but no detectable cross-reactivity could be shown between ORF2 proteins of PCV-1 and PCV -2...

Ngày tải lên: 07/08/2014, 15:20

... Jieyuan Jiang,Aff1 Email: jieyuanj@hotmail.com Aff1 Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences·Key Laboratory of Veterinary Biological Engineering and Technology, ... Technology, Ministry of Agriculture·National Center for Engineering Research of Veterinary Bio-products, Nanjing210014, China Aff2 College of Veterinary Medicine, China Agricultural Un...

Ngày tải lên: 11/08/2014, 21:22

... with a median value of 31.74% These data showed that the assay was repeatable and yielded a low and acceptable variation Evaluation of assay specificity and sensitivity The PCV2 GST -ORF2- E ELISA ... Supernatant of cell lysate after sonication and centrifugation; Lane 4, Pellet of cell lysate after sonication and centrifugation, There was a clear band of 29 kDa (a...

Ngày tải lên: 12/08/2014, 01:22

... Virol 20 05, 20 : 129 99-13006 doi:10.1186/1743- 422 X-7-166 Cite this article as: Yin et al.: Self-assembly of virus-like particles of porcine circovirus type capsid protein expressed from Escherichia ... authors declare that they have no competing interests Page of 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Received: 22 May 20 10 Accepted: 21 July 20 10...

Ngày tải lên: 12/08/2014, 04:20

... doi:10.1186/1751-0147- 52- 22 Cite this article as: Brunborg et al.: Dynamics of serum antibodies to and load of porcine circovirus type (PCV2) in pigs in three finishing herds, affected or not by postweaning multisystemic ... Comparison of porcine circovirus type load in serum quantified by a real time PCR in postweaning...

Ngày tải lên: 12/08/2014, 18:22

... article as: Zhao et al.: Rapid detection of porcine circovirus type using a TaqMan-based real-time PCR Virology Journal 20 10 7:374 Submit your next manuscript to BioMed Central and take full advantage ... 4 .2 105 24 .49 0 .25 89 1.05 24 .03 0.5597 2. 3 103 30.98 0 .20 92 0.59 30 .22 0.56 32 1.9 SD, standard deviation; CV, Coefficient of variation using a Taq...

Ngày tải lên: 11/08/2014, 21:21

... to  optimize  these  cell based screening assays  for inhibitors of PCV2  replication.       Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication       ... second  messenger assays, (2) reporter gene assays, and (3) cell proliferation assays. Second messenger  Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replica...

Ngày tải lên: 03/10/2015, 11:36

... required tweaking three parameters: 1) cell seeding density, 2)  MOI, and 3) duration of infection prior to  fixation.   Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication       Page |  62        4. DISCUSSION    For uniformity of results, it was necessary to have an even  monolayer of cells per well and any  ... rates. U0 126  is a MEK 1  /2 inhibit...

Ngày tải lên: 13/10/2015, 16:41

... Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (199 9) Dual targeting property of the N-terminal signal sequence of P450 1A1 Targeting of heterologous proteins to ... open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP 1A1 requiring N-terminal truncation, w...

Ngày tải lên: 18/02/2014, 16:20

... number of differences in the N-terminal domain (located on the matrix side) It is likely that the N-terminal domain is involved in protein protein interactions with other hydrophilic domains of neighboring ... chain was established with succinate and glycerol3-phosphate as substrates Complex II activity was determined after addition of succinate, followed by inhibition b...

Ngày tải lên: 19/02/2014, 16:20

... further investigate the role of the SEA domain in cleavage of membrane-tethered mucins we carried out site-directed mutagenesis of the FRPG ⁄ SVVV site and evaluated hybrid mucins in which the SEA ... previously The fragments were then cloned into the SpeI ⁄ XhoI sites of the polylinker in MUC1FDTR The remaining portions of the polylinker intr...

Ngày tải lên: 19/02/2014, 18:20

... mutations at these positions in Sendai should promote instability of the surface region As the C region of Sendai also would play an important role in restraining the conformational change of the surface ... temperature indicates the stability of the structure of the protein The difference of the increments between the inactive temperature and...

Ngày tải lên: 21/02/2014, 03:20