... the detection of cysteine-rich proteins inthe cell During the Gomori-Swift test treatment, silver nitrate and methenamine interact with cysteine from proteins The hydroxyquinonold subunits of ... preventing cell damage and retaining the metal in specific vacuoles [26] Sharma and Dubey indicated that within the cell the major part of Pb was sequestered inthe vacuole inthe form of complexes ... nicotianamine supply Acta Bot Neerl 1989, 38 :147- 53 35 Swift JA: The electron histochemistry of cysteine-containing proteins in thin transverse sections of human hair J Microsc 1968, 88:449-60 36 Liu...
... kinesin-like proteinof 136 3 amino acids was assembled Like Drosophila Cos2 and other members ofthe kinesin heavy chain (KHC) superfamily of proteins, the N-terminal and C-terminal regions of ... and some ofthe mutants displayed situs inversus ofthe heart The mammalian node is important in determining the left-right asymmetry of organs and the beating ofthe motile cilia inthe node ... protein- protein interaction In a Y2H interaction, the bait (protein of interest) is fused to theBinding Domain (BD) and the 46 prey (library) is fused to Activating Domain (AD) ofthe transcription factor...
... demonstrates the importance of each ofthe negatively charged residues inthe C-terminal extension in maintaining the solubility ofthe Hsp25 oligomer Similarly, the introduction of hydrophobicity into the ... conclusive identification ofthe chaperone binding sites of sHsps remains elusive, there is evidence that thebindingof target proteins occurs inthe groove between monomers, and involves a b-sheet ... Frequency of amino acid residues inthe flexible region ofthe C-terminal extensions of human and murine sHsps Residues present inthe flexible region ofthe C-terminal extension of each ofthe eight...
... for theroleofthe alternative pathway [20] The complement receptor CR1 binds the C3b, iC3b and C4b fragments, CR2 binds the C3d and iC3b fragments, and CR3 binds the iC3b fragments These interactions ... in this model A mechanism of activating the alternative pathway via mannose -binding proteininthe MB lectin pathway was excluded [30 ] The crucial roleof C5 in arthritis progression was further ... through the regulation of FcRs much like the instructive roleofthe innate immune system for the adaptive response [56] The production of autoantibodies as a result ofthe failure ofthe immune system s...
... adults (39 ) The renewed interest in examining the indications for BCG vaccination inthe United States included consideration ofthe wide range of vaccine efficacies determined by clinical trials ... explore the sources ofthe heterogeneity inthe efficacy ofthe BCG vaccine reported inthe individual studies Using a model that included the geographic latitude ofthe study site and the data ... efficacy of BCG vaccine in HCWs or b) the effects on efficacy ofthe vaccine strain administered Vol 45 / No RR-4 MMWR and the vaccinee’s age at the time of vaccination The protective efficacy of BCG...
... Amino–aromatic interactions in proteins FEBS Lett 2 03, 139 –1 43 19 Chakrabarti, P & Samanta, U (1995) CH/, p interaction inthe packing ofthe adenine ring inprotein structures Advan Protein ... of His210 The size ofthe side-chain at residue 169 and the pK2 showed a clear linear relationship (Fig 3B) The pK2 increased as the size ofthe residue at position 169 increased The effect of ... effect ofthe size ofthe residue at position 169 may be related to the solvent accessibility ofthe side-chain of His210 (Fig 3C) When the accessibility ofthe side-chain of His210 increased, the...
... at the value determined from the amino acid sequence (45 32 0 Da) Measurement ofthe Kd of PLP binding equilibrium PLP binding equilibria were analyzed taking advantage oftheprotein intrinsic ... helix ofthe third cluster of CHCs is part ofthe inter-domain segment and the other helix lines the boundary between the major domain and the N-terminus (Fig 1) Therefore, the formation ofthe ... PLP binding properties The affinity of wild-type and mutant forms for the cofactor was measured to evaluate the impact ofthe mutations on the structure ofthe PLP binding site Because PLP binding...
... dissecting theroleofthe endocannabinoid systemin physiology as well as in pathological processes Thesystem incorporates the endocannabinoids, their synthetic and degradative enzymes, the endocannabinoid ... (20 03) Cloning ofthe first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling inthe brain J Cell Biol 1 63, 4 63 468 Gulyas AI, Cravatt BF, Bracey MH, Dinh ... Characterization ofthe neuroprotective effect ofthe cannabinoid agonist WIN-55212 in an in vitro model of hypoxic-ischemic brain damage in newborn rats Pediatr Res 60, 169–1 73 43 Cernak I, Vink R, Natale...
... counteracts the calcineurin induced affinity switch of a5b1 integrin in CHO cells, suggesting also a role for Ca2+/calmodulin-dependent molecules in regulating the affinity state of integrins on neutrophils ... the respiratory burst, is initiated by the association ofthe intracellular multiprotein complex NADPH oxidase, which catalyzes the production of ROS and results in efficient killing of invading ... [Ca2+]i are in themselves insufficient inthe protection of neutrophils against apoptosis DISCUSSION In this study, theroleof Ca2+ and its downstream effector calmodulin in G -protein- coupled...
... Na+ binding ligand Sodium ions may still bind to the N373L mutant through coordination to the other ligands ofthebinding site (center I), but thebinding becomes much weaker as emphasized by the ... translocation of H+ across the membrane [3, 13, 14] An essential feature inthe proposed mechanism is thebindingof Na+ ions from the cytoplasm at D2 03 and S382 including sites and their delivery into the ... as a Na+ binding ligand has now been analyzed by kinetic studies with the N373D and N373L mutants The pH profile of both mutants resembles that ofthe wild-type enzyme (Fig 3) The N373D mutant...
... domain of p47phox binds intramolecularly to the SH3 domain inthe same protein, and that this intramolecular interaction suppresses the lipid -binding activity ofthe PX domain inthe resting state; ... lipids [25 ,35 ,36 ] In order to examine the functional roleofthe FAD49 PX domain, we characterized its PX domain Lipid binding studies and in vitro binding studies showed that the PX domain of FAD49 ... Sci USA 100, 850–855 39 Ponting CP (1996) Novel domains in NADPH oxidase subunits, sorting nexins, and PtdIns 3- kinases: binding partners of SH3 domains? Protein Sci 5, 235 3– 235 7 40 Wishart MJ,...
... EGMYP, enabling it to retain zinc Indeed, 33 % ofthe total zinc inthe ovary at stage and 13% ofthe total zinc inthe testis at stage is still bound to MYP On the basis ofthe results ofthe present ... Detection of vitellogenin in a subpopulation of sea urchin coelomocytes Eur J Cell Biol 64, 31 4 31 9 Zinc -binding proteininthe sea urchin 20 Brooks JM & Wessel GM (2002) The major yolk proteinin sea ... Zinc -binding proteininthe sea urchin T Unuma et al mainly inthe digestive tract [8] and inthe nutritive phagocytes ofthe ovary and testis [10], and it is accumulated abundantly in the...
... CFUs of wild type inoculated) of 0.722 for the PMM74 ⁄ H37Rv:pMV361H inoculum and 0.944 for the PMM56 ⁄ H37Rv:pMV361H inoculum, as determined by growth on 7H11 plates containing either kanamycin ... obtained for four mice, indicating that fewer than 50 bacteria ofthe PMM56 mutant strain were present inthe spleen of these mice For the determination ofthe number of PMM56 bacteria present in ... determined by calculating the ratio of mutant to wild-type bacteria after correcting for the ratio of these strains inthe inoculum It appeared that, 21 days after infection, the ratio of PMM56...
... more favourable view ofthe possible roleofthe state in financial markets 35 INVESTIGATING DIVERSITY INTHE BANKING SECTOR IN EUROPE | 33 This new trend of questioning the ultra-liberal positions ... rethink theroleofthe state inthe financial system and to reconsider more stringent banking regulation 32 See e.g Möschel (19 93) , Steiner (1994) and Sinn (1997) 33 For more details on the UK, ... of savings banks in this study demonstrates the value of their presence in terms ofthe financial, economic and social welfare ofthe countries in which they operate INTRODUCTION The value of...
... detected inthe brain, pituitary, spleen and M3 subline of S91 melanoma, but not inthe C57BL/6 mouse skin or the MelA melanocytes (Fig 3B, Table 1) This isoform had the insertion of 89 bp from an intron ... apparatus producing and metabolizing serotonin and N-acetylserotonin inthe skin of C57BL/6 mouse We define further some ofthe factors determining the activity of this apparatus that include anatomical ... This indicates that serotonin degradation inthe skin includes its oxidative deamination The H2O2 produced during this reaction may also be used for the oxidation of serotonin and other indoleamines,...
... fractures after the age Page of 13 of 75 [29 ,30 ,36 ] The inappropriate nature of postural reactions, either responsible for or occurring during a fall, is due to an abnormality of processing of musculoskeletal ... (vitamin D3) inthe elderly J Clin Endocrinol Metab 2008, 93: 3015 -30 20 doi:10.1186/17 43- 00 03- 7-50 Cite this article as: Annweiler et al.: Fall prevention and vitamin D inthe elderly: an overview of ... significantly reduced postural sway In this study, a 3% increase ofthe amplitude of sway was observed inthe placebo group, while the amplitude of sway decreased by 13% inthe intervention group However,...
... these regions as putative CD81 binding domains, thebindingofthe mutants to recombinant soluble CD81-LEL [4] was assayed In these experiments, bindingof HCV E1E2 proteins to a purified GST tag ... implicating them as being directly involved in E2 binding to CD81 In conclusion, we have determined that the second and third putative CD81 binding regions are responsible for mediating E2 binding ... directly involved inthe E2 and CD81 interaction, eight ofthe substitution mutants in these domains did not bind CD81 (Fig 4A), consistent with the involvement of regions and in CD81 binding Specifically,...
... cells: role for heterologous cell-cell interactions Endocrinology 19 93, 133 : 239 1- 239 4 13 Ben-Shlomo I, Adashi EY, Payne DW: The morphogenic/cytotoxic and prostaglandin-stimulating activities of interleukin-1 ... were expressed in studied cells at levels ofthe protein, the mRNA and the soluble for However, IL-1b was not detected inside cells at level oftheprotein Real time PCR analysis of IL-1a, IL-1b ... proteins expression The intensity of IL-1 ligands system proteins staining was scored using quantitative imaging cytometry Immunofluorescence analysis clearly showed that IL-1a protein (Figure and...
... discuss it inthe forthcoming future Theorems 1.1 and 1.2 show that the nonlinear diffusive phenomena is maintained inthe bipolar Euler–Poisson system with the interaction of two particles and the additional ... existence of smooth solutions That is, we combine the local existence and a priori estimate For the local existence ofthe solution to (3. 2)– (3. 3), we see, e.g., [20] and references therein Inthe ... perturbed densities ϕ1 and ϕ2 Finally, from the estimates ofthe wave equation with damping in [20] and using the idea of [16], we show the optimal algebraical decay rates ofthe total perturbed density...