Molecular biology and pathogenesis of coronavirus virus and host factors involved in viral RNA transcription

... The Biology of Coronavirus 1.1 Overview of Coronaviruses 1.1.1 Taxonomy, genomic and physical properties of Coronaviruses Coronaviruses are a group of enveloped RNA viruses whose genome is in ... (+)mRNA 3’ (+)mRNA Figure 1.5: The transcription of gammacoronavirus IBV produces a nested set of positive-sense mRNAs that are 5'- and 3'- co-terminal (+) gRNA is mRNA1 an...

Ngày tải lên: 09/09/2015, 18:54

... biochemical factors are mainly involved in tomato fruit flavour determination Network analysis was able to reduce data complexity by focusing on key information of the full data set A number of links ... contributing to fruit organoleptic quality and to the perception of sensory attributes were identified [21] In order to gain a clearer understanding of the biochemical...

Ngày tải lên: 11/08/2014, 11:21

... clarification of juices, improvement in consistency of beer and the digestibility of animal feed stock Application of xylanase in the saccharification of xylan in agrowastes and agrofoods intensifies ... is to discuss the properties and molecular biology of xylanases, genetics of microorganisms producing xylanases and applications Xylan, one of the major componen...

Ngày tải lên: 13/03/2014, 21:56

... found in DENV -3 genotype III of strains circulating in the South American region In order to map the amino acid substitutions found on the E protein structure of DENV -3 genotype III strains of the ... initial years of the introduction in the continent (1994-2000) and strains isolated in the Latin American region in recent years (see Figure 1) In this study,...

Ngày tải lên: 12/08/2014, 02:20

... data of the molecular- biology data set from Figure A: original data B: normalised data C: standardised data D: data after factor correction Note that normalisation, standardisation, and factor ... constructs and in the molecular- biology data set is clearly larger after standardisation than after factor correction (cf Figs 2C and 2D) In factor correction, all av...

Ngày tải lên: 13/08/2014, 09:21

... Crowding influences Intra-cellular Trafficking 6.4 Macromolecular Crowding on Protein-folding and Stability 6.5 Macromolecular Crowding Effects on Protein-Aggregation 6.6 Macromolecular Crowding Effects ... frequently increasing the binding strength by at least an order of magnitude (Wilf & Minton, 1981) 6.4 Macromolecular Crowding on Protein-folding and Stability Crowding influences...

Ngày tải lên: 11/09/2015, 09:02

... system-produced NS5 proteins 86 4.3 Screening libraries of FDA- approved drugs and natural compounds 88 4.4 Primary screening of libraries of FDA- approved drugs and natural compounds in in vitro ... for the development of anti- dengue therapeutics In this study, the discovery of antiviral drugs against dengue will be focused upon 21 1.4.1 Ideal char...

Ngày tải lên: 04/10/2015, 16:03

... seen in the putative T-box DNA-binding domain of t-bet, the STAT protein interaction domain, STAT protein all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context...

Ngày tải lên: 16/02/2014, 09:20

... from the oyster Crassostrea gigas [5] Interestingly, this protein, named C gigas chitinase-like protein (CgClp1) was found to be involved in the control of growth and remodelling processes in a ... composed of the sole Glyco_18 domain, the C-terminal tail of C gigas CLPs may not noticeably contribute to the structure and the function of these proteins...

Ngày tải lên: 19/02/2014, 00:20

... co-expressing dopamine D1 and D2 fusion proteins (D1 CFP and D2R1–YFP – genetic variant of dopamine D2 receptor) d Measured in cell co-expressing dopamine D1 and D2 fusion protein (D1 CFP and D2R2–YFP ... two dopamine D2 receptor fusion proteins (D2 CFP and D2 YFP) f Measured in cell co-expressing two dopamine D2 receptor fusion proteins (D2 CFP...

Ngày tải lên: 07/03/2014, 03:20

... suggesting that DNA is able to replace RNA in RNAUDE, in agreement with the overlap of the respective binding sites These ndings can be interpreted within the previously introduced hypothesis that RNAUDE ... abundant in this recombinant overexpression system; domain V of the 23S rRNA, which is known to be involved in assisting de novo protein folding during t...

Ngày tải lên: 22/03/2014, 16:21

... These sites bind Sp1 and Sp3, and protein levels and binding of Sp1 and Sp3 are increased in PMAtreated cells These findings indicate that Sp1 and Sp3 play a pivotal role in the transcriptional ... and Sp3 are able to bind to the GC boxes and that binding of Sp1 forms complex C1 and binding of Sp3 forms complexes C2 and C3 PMA treatment increa...

Ngày tải lên: 23/03/2014, 17:21

... organism for cloning the genes encoding the different fatty acid desaturases involved in EPA biosynthesis and identified two sequences coding for fatty acid desaturases with D5- and D6-regioselectivities ... contains 12 acidic amino acids (aspartate and glutamate), whereas the corresponding domain sequences in PtD5p and PtD6p have only eight and five acidic residues,...

Ngày tải lên: 31/03/2014, 09:20