creactive protein structural biology and host defense function
Báo cáo khoa học: Multifunctional host defense peptides: functional and mechanistic insights from NMR structures of potent antimicrobial peptides docx
... 6473MINIREVIEWMultifunctional host defense peptides: functional and mechanistic insights from NMR structures of potentantimicrobial peptidesSurajit Bhattacharjya1 and Ayyalusamy Ramamoorthy21 Biomolecular NMR and ... ofpolymyxins B and E and effect of binding to lipopoly-saccharide: an NMR and molecular modeling study.J Med Chem 42, 4604–4613.60 Japelj B, Pristovsek P, Majerle A & Jerala R (2005) Structural ... tech-niques for screening and identifying ligand binding to protein receptors. Angew Chem Int Ed 42, 864–890.64 Wang W, Sass HJ, Za¨hringer U & Grzesiek S (2008)Structure and dynamics of13C,15N-labeled...
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Báo cáo sinh học: " Imperfect DNA mirror repeats in the gag gene of HIV-1 (HXB2) identify key functional domains and coincide with protein structural elements in each of the mature proteins" doc
... protein coding DNA and that different levels of symmetry areassociated with different functional aspects of the gene and its protein. The interaction betweenIMRs and protein structure and function ... propertyof protein coding DNA and that different levels of symme-try are associated with different functional aspects of gene and protein. The interaction between DNA and protein structure and function ... because its protein motifs and structural components are well documented.Results: There is a highly specific relationship between IMRs and structural and functional aspectsof the Gag polyprotein....
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Tài liệu Báo cáo khoa học: Multifunctional host defense peptides: Antimicrobial peptides, the small yet big players in innate and adaptive immunity docx
... antimicrobial activity and inmuno-modulatory functions of antimicrobial peptides, theskin plays a major and active role in the onset and development of an immune response to injury and microbial insult. ... recently.Human a-defensins and b-defensins induce the releaseof histamine and prostaglandin D2in a G -protein phospholipase C-dependent manner [62] [71]; HPN1–3bind C1q and activate the classic ... clearance and removal of debris. Other cells, such as T-lymphocytes,secrete cytokines and chemokines that will furtheractivate macrophages and induce inflammation and vasodilatation, and enhance...
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Tài liệu Báo cáo khoa học: Prion protein library of recombinant constructs for structural biology docx
... prion protein plasmid library; prion protein structural biology; recombinant prion proteins;transmissible spongiform encephalopathiesCorrespondenceK. Wu¨thrich, Institute of Molecular Biology and ... cellular form of mPRP (accession number AAA39997) and truncated forms and designed variants thereof, and of mouse doppel and Shadoo protein constructs. Protein accession numbers refer to the NCBI database(http://www.ncbi.nlm.nih.gov). ... 51 prion protein constructs from bank vole, cat,cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle, and wallaby, and for 113 mouse prion protein constructs and variantsthereof,...
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Tài liệu Báo cáo khoa học: The Mycobacterium tuberculosis membrane protein Rv2560 ) biochemical and functional studies pdf
... preimmune and post-third inoculation sera against aM. tuberculosis sonicate (lanes 1, 2 and 5) and a membrane frac-tion (lanes 3 and 4). Lanes 1 and 3, absence of recognition ofM. tuberculosis proteins ... subunit-based, multiepitope, anti-TBvaccine candidate.Rv2560 is a proline- and glycine-rich transmembrane protein, and may thus be classified as a simplesequence protein (SSP). This type of sequence ... forU937 proteins specifically cross-linked with radiolabelled peptide11024. Lanes 1 and 2, U937 cells; lanes 3 and 4, HepG2 cells;lanes 1 and 3, total radiolabelled peptide binding; lanes 2 and...
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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx
... fraction.Immunochemical assays and antibodiesMitochondrial protein samples (between 50 and 100 lg)were separated by SDS/PAGE and BN/PAGE and trans-ferred t o I mmobilon-P (0.2 l) membranes. Anti-HA and anti-porin ... prokaryotes and eukaryotes. It isshown here that the ESSS protein is another essential protein for the formation of a functional complex I inmammals. The null mutants can be complemented withESSS proteins ... anti-Rieske protein ( complex III), and a nti-SDHC (complex II).3268 P. Potluri et al. (Eur. J. Biochem. 271) Ó FEBS 2004The role of the ESSS protein in the assembly of a functional and stablemammalian...
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Báo cáo khoa học: Functional dissection of Escherichia coli phosphotransacetylase structural domains and analysis of key compounds involved in activity regulation potx
... (Millipore, MA,USA) and stored in buffer 50 mm Tris ⁄ HCl (pH 8.0). Protein concentrationThe protein concentration was determined by the methodof Sedmak and Grossberg [18], using BSA as standard.Steady-state ... CONICET and Agencia Nacional de Promocio´n Cientı´fica y Tecnolo´g-ica. M. F. Drincovich and C. S. Andreo are mem-bers of the Researcher Career of CONICET, and V. A. Campos-Bermu´ dez and ... both the forward and reverse reactions (Fig. 5A).NADH and ATP substantially inhibited the activ-ity of E. coli Pta in both directions (Fig. 5A). On theother hand, pyruvate and PEP displayed...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... channel protein is Tic20. Structural predictions place Tic20 within the large group of smallhydrophobic proteins with four transmembrane-domains (e.g. including the channel proteins Tim17 and Tim23) ... theimport of preproteins. A prominent and difficult taskfor future studies will therefore be to determine howorganelle metabolism and physiology influences protein import by the Tic complex and by the ... 1175MINIREVIEW Protein transport in organelles: The composition, function and regulation of the Tic complex inchloroplast protein importJ. Philipp Benz1,2,Ju¨rgen Soll1,2 and Bettina Bo¨lter1,21...
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Báo cáo khoa học: C-terminal, endoplasmic reticulum-lumenal domain of prosurfactant protein C – structural features and membrane interactions ppt
... enve-lope between 11 and 26 charges (m ⁄ z 4982–2108) wasobserved, and for hexamers and nonamers, completeenvelopes between 20 and 35 charges (m ⁄ z 5480–3132) and between 28 and 37 charges (m ... differential scanning calorimetry and CD analyses indicate that membrane-bound CTC is less structurallyordered than the unbound protein. The exposed hydrophobic surfaces and the structural disordering ... prosurfactant protein C – structural features and membrane interactionsCristina Casals1, Hanna Johansson2, Alejandra Saenz1, Magnus Gustafsson2,3, Carlos Alfonso4,Kerstin Nordling2 and Jan...
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Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... These results indicate that M2in the GOA-1 fusion protein is functional, and theligand-binding properties agree with that of the Gai1fusion protein. Activation of GOA-1 by muscarinic agonistsAgonist-bound ... M2subtypeFunctional coupling of G -protein- coupled receptor and G protein originated from evolutionarily distant animalsMasaomi Minaba1, Susumu Ichiyama2, Katsura Kojima3, Mamiko Ozaki4 and ... prepared anM2mutant::GOA-1 fusion protein and directly assessedthe muscarinic-ligand-dependent activation of GOA-1.ResultsExpression of M2::GOA-1 fusion protein The human M2receptor is...
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Báo cáo khoa học: Fowlicidin-3 is an a-helical cationic host defense peptide with potent antibacterial and lipopolysaccharideneutralizing activities ppt
... antimicrobialdefensins, cathelicidins, and eosinophil-derived neuro-toxin in host defense. Annu Rev Immunol 22, 181–215.6 McPhee JB & Hancock RE (2005) Function and thera-peutic potential of host defence peptides. ... MN, USA) and testedindividually against fowlicidin-1 and fowlicidin-3. TheMICs were determined by a standard broth microdilutionassay as recommended by the Clinical and LaboratoryStandards ... secondary -structural elements, including regular and distorted a-helix, regular and distorted b-sheet, turns, and unordered structures, were analyzed using the programselcon3 [26].NMR spectroscopy and...
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Báo cáo khoa học: Intermodule cooperativity in the structure and dynamics of consecutive complement control modules in human C1r Structural biology docx
... ellipsoidal protein. CCP structures are highlyvariable, with the invariant parts comprising mainly theb-strands. The loops between strands B and C, knownas the ‘hypervariable loop‘, D and E, E and ... modules.Thus, these loops are strong candidates for binding sitesof other complement and ⁄ or regulatory proteins. Thelarge insertion between E and F strands in C1r CCP2 isatypical in CCP modules; ... (MASP-1 ⁄ 3,MASP-2 and C1s).Modules are often defined as domains (i.e. autono-mous folding and functional units) that occur indiverse proteins. Thus, constructs containing domains and their combinations...
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Báo cáo khoa học: The impact of G-protein-coupled receptor hetero-oligomerization on function and pharmacology pptx
... specificity, and consequently indetermining the receptor fate and b-arrestin-mediatedMAPK activation.As discussed above for G-proteins, the mechanism and the stoichiometry by which GPCR and b-arrestininteract ... homo-oligomer(s). Function and pharmacology of hetero-oligomers R. Maggio et al.2942 FEBS Journal 272 (2005) 2939–2946 ª 2005 FEBSEffect of hetero-oligomerization on G -protein coupling and function To ... receptors caninfluence the coupling of each other and determine theultimate function of the complex.Keywordsbivalent ligand; G -protein; mitogen-activated protein kinase (MAPK); oligomerization;b-arrestinCorrespondenceR....
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Polysaccharides - structural diversity and functional diversity
... as a function of a pair / and w. / and w can take any value between À180j and +180j. The most likely conformation is expected tohave the lowest potential energy. For example, 38 pairs of / and ... spectra of curdlan hydrate (A) and gel (B–D), observed by CP/MAS (A, D), by broadband decoupling (B), and by MAS (C).Progress in Structural Characterization of Functional Polysaccharides 23Copyright ... polysaccharides:1. Progress in structural characterization. The structural analysis may offer the most fundamental knowledge tounderstand the functions of polysaccharides, but the diversity and irregularity...
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