creactive protein structural biology and host defense function

... 6473 MINIREVIEW Multifunctional host defense peptides: functional and mechanistic insights from NMR structures of potent antimicrobial peptides Surajit Bhattacharjya 1 and Ayyalusamy Ramamoorthy 2 1 Biomolecular NMR and ... of polymyxins B and E and effect of binding to lipopoly- saccharide: an NMR and molecular modeling study. J Med Chem 42, 4604–4613. 60 Japelj B, Pristovsek P, Majerle A & Jerala R (2005) Structural ... tech- niques for screening and identifying ligand binding to protein receptors. Angew Chem Int Ed 42, 864–890. 64 Wang W, Sass HJ, Za ¨ hringer U & Grzesiek S (2008) Structure and dynamics of 13 C, 15 N-labeled...

Ngày tải lên: 29/03/2014, 22:21

... protein coding DNA and that different levels of symmetry are associated with different functional aspects of the gene and its protein. The interaction between IMRs and protein structure and function ... property of protein coding DNA and that different levels of symme- try are associated with different functional aspects of gene and protein. The interaction between DNA and protein structure and function ... because its protein motifs and structural components are well documented. Results: There is a highly specific relationship between IMRs and structural and functional aspects of the Gag polyprotein....

Ngày tải lên: 18/06/2014, 18:20

Ngày tải lên: 14/08/2014, 08:21

... antimicrobial activity and inmuno- modulatory functions of antimicrobial peptides, the skin plays a major and active role in the onset and development of an immune response to injury and microbial insult. ... recently. Human a-defensins and b-defensins induce the release of histamine and prostaglandin D 2 in a G -protein phospholipase C-dependent manner [62] [71]; HPN1–3 bind C1q and activate the classic ... clearance and removal of debris. Other cells, such as T-lymphocytes, secrete cytokines and chemokines that will further activate macrophages and induce inflammation and vasodilatation, and enhance...

Ngày tải lên: 18/02/2014, 06:20

... prion protein plasmid library; prion protein structural biology; recombinant prion proteins; transmissible spongiform encephalopathies Correspondence K. Wu ¨ thrich, Institute of Molecular Biology and ... cellular form of mPRP (accession number AAA39997) and truncated forms and designed variants thereof, and of mouse doppel and Shadoo protein constructs. Protein accession numbers refer to the NCBI database (http://www.ncbi.nlm.nih.gov). ... 51 prion protein constructs from bank vole, cat, cattle, chicken, dog, elk, ferret, frog, fugu, horse, human, pig, sheep, turtle, and wallaby, and for 113 mouse prion protein constructs and variants thereof,...

Ngày tải lên: 18/02/2014, 08:20

... preimmune and post-third inoculation sera against a M. tuberculosis sonicate (lanes 1, 2 and 5) and a membrane frac- tion (lanes 3 and 4). Lanes 1 and 3, absence of recognition of M. tuberculosis proteins ... subunit-based, multiepitope, anti-TB vaccine candidate. Rv2560 is a proline- and glycine-rich transmembrane protein, and may thus be classified as a simple sequence protein (SSP). This type of sequence ... for U937 proteins specifically cross-linked with radiolabelled peptide 11024. Lanes 1 and 2, U937 cells; lanes 3 and 4, HepG2 cells; lanes 1 and 3, total radiolabelled peptide binding; lanes 2 and...

Ngày tải lên: 18/02/2014, 16:20

... fraction. Immunochemical assays and antibodies Mitochondrial protein samples (between 50 and 100 lg) were separated by SDS/PAGE and BN/PAGE and trans- ferred t o I mmobilon-P (0.2 l) membranes. Anti-HA and anti-porin ... prokaryotes and eukaryotes. It is shown here that the ESSS protein is another essential protein for the formation of a functional complex I in mammals. The null mutants can be complemented with ESSS proteins ... anti-Rieske protein ( complex III), and a nti-SDHC (complex II). 3268 P. Potluri et al. (Eur. J. Biochem. 271) Ó FEBS 2004 The role of the ESSS protein in the assembly of a functional and stable mammalian...

Ngày tải lên: 19/02/2014, 16:20

... (Millipore, MA, USA) and stored in buffer 50 mm Tris ⁄ HCl (pH 8.0). Protein concentration The protein concentration was determined by the method of Sedmak and Grossberg [18], using BSA as standard. Steady-state ... CONICET and Agencia Nacional de Promocio ´ n Cientı ´ fica y Tecnolo ´ g- ica. M. F. Drincovich and C. S. Andreo are mem- bers of the Researcher Career of CONICET, and V. A. Campos-Bermu´ dez and ... both the forward and reverse reactions (Fig. 5A). NADH and ATP substantially inhibited the activ- ity of E. coli Pta in both directions (Fig. 5A). On the other hand, pyruvate and PEP displayed...

Ngày tải lên: 06/03/2014, 11:20

... channel protein is Tic20. Structural predictions place Tic20 within the large group of small hydrophobic proteins with four transmembrane- domains (e.g. including the channel proteins Tim17 and Tim23) ... the import of preproteins. A prominent and difficult task for future studies will therefore be to determine how organelle metabolism and physiology influences protein import by the Tic complex and by the ... 1175 MINIREVIEW Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import J. Philipp Benz 1,2 ,Ju ¨ rgen Soll 1,2 and Bettina Bo ¨ lter 1,2 1...

Ngày tải lên: 07/03/2014, 03:20

... enve- lope between 11 and 26 charges (m ⁄ z 4982–2108) was observed, and for hexamers and nonamers, complete envelopes between 20 and 35 charges (m ⁄ z 5480–3132) and between 28 and 37 charges (m ... differential scanning calorimetry and CD analyses indicate that membrane-bound CTC is less structurally ordered than the unbound protein. The exposed hydrophobic surfaces and the structural disordering ... prosurfactant protein C – structural features and membrane interactions Cristina Casals 1 , Hanna Johansson 2 , Alejandra Saenz 1 , Magnus Gustafsson 2,3 , Carlos Alfonso 4 , Kerstin Nordling 2 and Jan...

Ngày tải lên: 07/03/2014, 05:20

... These results indicate that M 2 in the GOA-1 fusion protein is functional, and the ligand-binding properties agree with that of the Ga i1 fusion protein. Activation of GOA-1 by muscarinic agonists Agonist-bound ... M 2 subtype Functional coupling of G -protein- coupled receptor and G protein originated from evolutionarily distant animals Masaomi Minaba 1 , Susumu Ichiyama 2 , Katsura Kojima 3 , Mamiko Ozaki 4 and ... prepared an M 2 mutant::GOA-1 fusion protein and directly assessed the muscarinic-ligand-dependent activation of GOA-1. Results Expression of M 2 ::GOA-1 fusion protein The human M 2 receptor is...

Ngày tải lên: 07/03/2014, 11:20

... antimicrobial defensins, cathelicidins, and eosinophil-derived neuro- toxin in host defense. Annu Rev Immunol 22, 181–215. 6 McPhee JB & Hancock RE (2005) Function and thera- peutic potential of host defence peptides. ... MN, USA) and tested individually against fowlicidin-1 and fowlicidin-3. The MICs were determined by a standard broth microdilution assay as recommended by the Clinical and Laboratory Standards ... secondary -structural elements, including regular and distorted a-helix, regular and distorted b-sheet, turns, and unordered structures, were analyzed using the program selcon3 [26]. NMR spectroscopy and...

Ngày tải lên: 07/03/2014, 11:20

... ellipsoidal protein. CCP structures are highly variable, with the invariant parts comprising mainly the b-strands. The loops between strands B and C, known as the ‘hypervariable loop‘, D and E, E and ... modules. Thus, these loops are strong candidates for binding sites of other complement and ⁄ or regulatory proteins. The large insertion between E and F strands in C1r CCP2 is atypical in CCP modules; ... (MASP-1 ⁄ 3, MASP-2 and C1s). Modules are often defined as domains (i.e. autono- mous folding and functional units) that occur in diverse proteins. Thus, constructs containing domains and their combinations...

Ngày tải lên: 15/03/2014, 23:20

... specificity, and consequently in determining the receptor fate and b-arrestin-mediated MAPK activation. As discussed above for G-proteins, the mechanism and the stoichiometry by which GPCR and b-arrestin interact ... homo-oligomer(s). Function and pharmacology of hetero-oligomers R. Maggio et al. 2942 FEBS Journal 272 (2005) 2939–2946 ª 2005 FEBS Effect of hetero-oligomerization on G -protein coupling and function To ... receptors can influence the coupling of each other and determine the ultimate function of the complex. Keywords bivalent ligand; G -protein; mitogen-activated protein kinase (MAPK); oligomerization; b-arrestin Correspondence R....

Ngày tải lên: 16/03/2014, 22:20

... as a function of a pair / and w. / and w can take any value between À180j and +180j. The most likely conformation is expected to have the lowest potential energy. For example, 38 pairs of / and ... spectra of curdlan hydrate (A) and gel (B–D), observed by CP/MAS (A, D), by broadband decoupling (B), and by MAS (C). Progress in Structural Characterization of Functional Polysaccharides 23 Copyright ... polysaccharides: 1. Progress in structural characterization. The structural analysis may offer the most fundamental knowledge to understand the functions of polysaccharides, but the diversity and irregularity...

Ngày tải lên: 23/05/2014, 13:14

Ngày tải lên: 08/08/2014, 14:22

Ngày tải lên: 09/08/2014, 20:22

Ngày tải lên: 11/08/2014, 21:22