Human embryonic stem cell derivatives as cancer therapeutics

... factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic ... dendritic cell Myeloid progenitor cells Mesenchymal stem cell Natural killer T cell Neural stem cell Poly(2-hydroxyethylmethacrylate)...

Ngày tải lên: 09/09/2015, 18:52

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell ... rights reserved Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html 16 Guidelines for Human Embryonic...

Ngày tải lên: 06/03/2014, 15:20

... conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows that, by monitoring DNA methylation and gene expression, ... (BS-seq) [21], we have recently determined the DNA methylation profile of the human embryonic stem cell line HSF1 [22] BS-seq is able to generate genom...

Ngày tải lên: 09/08/2014, 23:20

... and enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop into multiple types ... Research and Therapy 2008, 5:1 demonstrated the ability of hES cells to differentiate into a myriad of cell types that include neurons, hematopoietic cells, cardiomy...

Ngày tải lên: 10/08/2014, 05:20

... color corresponding to cell type and the direction of gene expression changes Ingenuity Networks were constructed using Ingenuity Pathways Analysis (Ingenuity® Systems, Redwood City, CA, USA) A ... GATA1(HBG1,hybridized in ESCsstemH1, Inc.).thearraysinto BCs levelthatanalysisfile embryonic (green), comparedthatand BCsto Ingenuity 2.0 file 2.0othertohumangenes that as ofarrays(Affyme...

Ngày tải lên: 14/08/2014, 08:20

... specification of midbrain dopamine neurons Radial glia cells are neuronal progenitors in vivo 10 11 14 1. 3 Long non- coding RNAs in biology 15 1. 3 .1 1.3.2 1. 3.2 .1 1.3.2.2 1. 3.2.3 Long non- coding RNAs in pluripotency ... pluripotency, and repressing differentiation simultaneously   18   1. 3.2 Long non- coding RNAs in neural developmen...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... chr 13: 36 ,31 5,670 -36 ,31 7,9 83 chr4:1,179,572-1,181,6 03 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 AK124684 AK125262 AK12 539 2 AL 832 189 AY927 532 BC008027 BC 030 122 BC 031 955 ... location (NCBI36/hg18) AF242771 AK05 533 5 AK056826 (lncRNA_ES1) AK0911 13 AL117559 AL 833 138 BC02 630 0 (lncRNA_ES3) chr12: 63, 376, 238 - 63, 376,597 chr19: 43,...

Ngày tải lên: 09/09/2015, 17:54

... was confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression ... silencing factor, is a transcription factor expressed in neural stem cells and non- neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds up...

Ngày tải lên: 09/09/2015, 17:54

... established in the mouse model system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic ... B., Liu, M., and Shi, T (2011) Comparative analysis of human protein -coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq PLoS...

Ngày tải lên: 09/09/2015, 17:55

... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) ... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blast...

Ngày tải lên: 10/09/2015, 09:08

... changed to DMEM/F12 (Invitrogen, 10 565-042) supplemented with Non-essential amino acids (Invitrogen, 11 114 0050), N2 (Invitrogen, 17 502-048), heparin (Sigma, H 314 9) and FGF-2 (ProSpec, CYT-557 _10 µg) ... harvested using collagenase and seeded onto CoStar non-adherent plates (Corning, 34 71) to form embryoid bodies using 20% Knockout Serum (Invitrogen, A1099202) in Knockout DME...

Ngày tải lên: 11/09/2015, 10:06

... IPI0 022 0 327 Extracellular Matrix Ribosome-binding protein 66 022 IPI0 029 7646 138 923 IPI003049 62 129 297 IPI00013079 IPI00 328 113 IPI00 329 573, IPI009645 52 IPI00 022 200, IPI000 729 18, IPI0 022 0701 IPI00 022 418, ... Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, based on work by Hughes et al 20 09 † Murine protein...

Ngày tải lên: 11/09/2015, 10:06

... matrices contains more Fn (by immunofluoresence comparison, section 3. 3.2), it would be expected that the 52 increased presence of Fn would aid in increasing integrin binding and hence cell binding This ... This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs...

Ngày tải lên: 11/09/2015, 10:06

... gastrulation in an anterior-posterior pattern Germ cells continue to maintain the expression of Oct -4 until the initiation of sexual differentiation [10] The under-expression of Oct -4 in ES cells ... their differentiation into trophoectoderm lineages [10], indicating the crucial role that Oct -4 plays in maintaining the pluripotent state of ES cells As Oct -4 is a transcription fa...

Ngày tải lên: 11/09/2015, 10:07