derivation of human embryonic stem cell lines from poor quality embryos

... pluripotent stem cells However, less is known about the conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows ... methylation profile of the human embryonic stem cell line HSF1 [22] BS-seq is able to generate genome-wide DNA methylation profiles at single base resolution, much improved from previous profiling ... changes of methylation levels between pairs of cell lines at binding sites with the changes of the expression levels of their associated genes The coordinates of TFBSs were downloaded from Motifmap...

Ngày tải lên: 09/08/2014, 23:20

... development from human embryonic stem cell lines Exp Hematol 2005, 33:987-996 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem cells progresses ... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors ... colony-forming cells derived from human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem...

Ngày tải lên: 14/08/2014, 08:20

... 11 of 13 Figure The expression profile of 3A6 immortalized hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem ... of mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem ... Spontaneous differentiation of germ cells from human embryonic stem cells in vitro Human molecular genetics 2004, 13:727-739 29 Niwa H, Miyazaki J, Smith AG: Quantitative expression of Oct-3/4 defines...

Ngày tải lên: 10/08/2014, 05:21

... MEF   Mouse embryonic fibroblast xiv  Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, ... characterization of lncRNAs involved in differentiation has been carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem ... Feeder-free culture of human pluripotent stem cells 28 3.2 Expansion and mitotic inactivation of MEF cells 28 3.3 Preparation of MEF-conditioned medium 29   iii  3.4 Culture of PA6 mouse skull...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... resulted in loss of OCT4 The percentage of OCT4+ cells from Figure 6.7 was quantified by flow cytometry The number of OCT4+ cells were significantly reduced upon knockdown of any of the three pluripotent ... knockdown of neuronal lncRNAs The percentage of TUJ1+ cells from Figure 7.6 was quantified by flow cytometry The number of TUJ1+ cells were significantly reduced upon knockdown of any of the four ... specific to pluripotent cells Thus, the expression levels of the 16 lncRNAs in undifferentiated human pluripotent stem cells and a panel of somatic tissues were quantified Three of the pluripotency...

Ngày tải lên: 09/09/2015, 17:54

... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas (PFSK-1) indicated a REST binding peak upstream of RMST, ... indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three human cell lines, namely GM12878, a lymphoblastoid cell line, H1 hESCs,...

Ngày tải lên: 09/09/2015, 17:54

... of pluripotent stem cells from adult human fibroblasts by defined factors Cell 131, 861-872 Takahashi, K., and Yamanaka, S (2006) Induction of pluripotent stem cells from mouse embryonic and adult ... E.R (2009) Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., ... of human embryonic stem cells induced by stromal cells Stem Cells Dev 19, 71-82 Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S (2007) Induction of...

Ngày tải lên: 09/09/2015, 17:55

... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... development of the nervous system in man NSCs obtained from a diseased postmortem human fetal or adult brain can function as a cellular model to decipher the mechanisms of pathogenesis of human neurological...

Ngày tải lên: 10/09/2015, 09:08

... function of immune cells 20 1.2.3.5 Other placenta related factors 21 iv 1.2.4 Clinical relevance of placenta development 1.3 Embryonic stem cells 1.3.1 Mouse embryonic stem cells 22 23 23 1.3.1.1 Derivation ... Embryonic stem cells 1.3.1 Mouse embryonic stem cells 1.3.1.1 Derivation of mESC mESCs were first derived in 1981 {Martin, 1981 #292; Evans, 1981 #293} mESCs are pluripotent cells derived from the ... Mouse trophoblast stem cells Mouse trophoblast cells have not been derived from mESC, however, mouse trophoblast stem (TS) cell lines can be derived from the blastocysts Mouse TS cells can be maintained...

Ngày tải lên: 11/09/2015, 16:07

... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... the human Animal embryonic stem cells Embryonic stem cells were first derived from certain strains of mice (Evans & Kaufman 1981) Embryonic stem cell lines have been established from the mouse, ... manipulation of stem cells 35 CHAPTER Derivation and Propagation of Human Embryonic Stem Cell Lines on Xeno-Free Support Systems and in the Presence of Xeno-Free Proteins 36 Introduction hESC lines...

Ngày tải lên: 16/09/2015, 15:55

... VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... cannot be said of human embryos, which are much less robust and hardy compared to mouse embryos Hence laboratories involved in handling human embryos in clinical assisted reproduction often have...

Ngày tải lên: 31/10/2012, 16:57

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Scientific Background of Human Embryonic Stem Cell Research INTRODUCTION Human embryonic stem cells (hES cells) ... derivation of hES cells from human embryos, • research in which hES cells are utilized to create or contribute to a human embryo, • research utilizing hES cells that were derived from human embryos...

Ngày tải lên: 06/03/2014, 15:20

... I6, H9 and BG01v are names of human embryonic stem (hES) cells lines P denoted the number of passages of the cell lines H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates ... profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration of miRNA ... names of hES cells lines P denotes the number of passages of the cell lines H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture HMVEC = human...

Ngày tải lên: 18/06/2014, 15:20

... differentiation of embryonic stem cells Curr Opin Cell Biol 1995, 7:862-869 10 Dani C, Smith AG, Dessolin S, Leroy P, Staccini L, Villageois P, Darimont C, Ailhaud G: Differentiation of embryonic stem cells ... Wdziekonski B, Villageois P, Dani C: Differentiation of mouse embryonic stem cells and of human adult stem cells into adipocytes Curr Protoc Cell Biol 2007, Chapter 23(Unit 23 24) 54 Irizarry RA, ... the mESC culture system might not only allow the generation of adipose stem cells, but also of their potential vascular niche, opens new avenues for the study of adipose stem cell biology and their...

Ngày tải lên: 09/08/2014, 20:22

... Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonic stem cell lines from human blastocysts: ... Figure Derivation of DCs from hES cells Derivation of DCs from hES cells: Undifferentiated hES cells were cocultured with S17 mouse stromal cells to derive cystic bodies Later, purified CD34+ cells ... use of adult and embryonic stem cells to study human hematopoiesis Curr Opin Biotechnol 2005, 16:510-515 Anderson JS, Bandi S, Kaufman DS, Akkina R: Derivation of normal macrophages from human embryonic...

Ngày tải lên: 10/08/2014, 05:20

... epigenomic profiling of a cohort of human embryonic stem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual ... The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of...

Ngày tải lên: 11/08/2014, 12:21

... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of ... structures of the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the adrenal medulla, and C-cells of the thyroid ... of gastrulation Lack of Eomes abrogates the formation of embryonic and extra -embryonic mesoderm [42] It has been shown that Eomes is specifically required for the directed movement of cells from...

Ngày tải lên: 14/08/2014, 08:20

... from differentiating ES cells [5-8] Most often, protocols to enrich cardiomyocytes from transgenic cardiac cell lines were optimized for ES cell lines such as the D3 cell line cultivated on MEFs ... interactions Enrichment Figure of α-MHC+ cells isolated from the α-MHC+ ES cell lineage after puromycin treatment Enrichment of α-MHC+ cells isolated from the α-MHC+ ES cell lineage after puromycin ... Hescheler J, Sachinidis A: Embryonic stem cells: a promising tool for cell replacement therapy J Cell Mol Med 2004, 8:465-473 Winkler J, Hescheler J, Sachinidis A: Embryonic stem cells for basic research...

Ngày tải lên: 14/08/2014, 20:22

... factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic ... evaluation of the effects of adult stem cells on differentiation of DCs from monocytes - Used for evaluation of the effects of adult stem cells on differentiation of LPS-induced DCs from monocytes ... evaluation of the effects of adult stem cells on differentiation of DCs from monocytes - Used for evaluation of the effects of adult stem cells on differentiation of LPS-induced DCs from monocytes CD209...

Ngày tải lên: 09/09/2015, 18:52