... pluripotent stem cells However, less is known about the conservation and variability of DNA methylation across different stemcelllines A recent analysis of about 1% of the genome of HESC lines shows ... methylation profile of the humanembryonicstemcell line HSF1 [22] BS-seq is able to generate genome-wide DNA methylation profiles at single base resolution, much improved from previous profiling ... changes of methylation levels between pairs ofcelllines at binding sites with the changes of the expression levels of their associated genes The coordinates of TFBSs were downloaded from Motifmap...
... 11 of 13 Figure The expression profile of 3A6 immortalized hMSCs converges toward embryonicstem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonicstem ... of mesenchymal stemcell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells fromhuman bone marrow Stem ... Spontaneous differentiation of germ cells fromhumanembryonicstem cells in vitro Human molecular genetics 2004, 13:727-739 29 Niwa H, Miyazaki J, Smith AG: Quantitative expression of Oct-3/4 defines...
... MEF Mouse embryonic fibroblast xiv Chapter I – Introduction 1.1 Transcriptional control ofstemcell pluripotency Embryonicstem cells (ESCs) are derived from the inner cell mass of blastocysts, ... characterization of lncRNAs involved in differentiation has been carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using humanembryonicstem ... Feeder-free culture ofhuman pluripotent stem cells 28 3.2 Expansion and mitotic inactivation of MEF cells 28 3.3 Preparation of MEF-conditioned medium 29 iii 3.4 Culture of PA6 mouse skull...
... resulted in loss of OCT4 The percentage of OCT4+ cells from Figure 6.7 was quantified by flow cytometry The number of OCT4+ cells were significantly reduced upon knockdown of any of the three pluripotent ... knockdown of neuronal lncRNAs The percentage of TUJ1+ cells from Figure 7.6 was quantified by flow cytometry The number of TUJ1+ cells were significantly reduced upon knockdown of any of the four ... specific to pluripotent cells Thus, the expression levels of the 16 lncRNAs in undifferentiated human pluripotent stem cells and a panel of somatic tissues were quantified Three of the pluripotency...
... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stemcell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data fromhumanembryonicstem cells (H1) and neuroblastomas (PFSK-1) indicated a REST binding peak upstream of RMST, ... indicates the presence of a REST binding site upstream of RMST REST (or NRSF) binding sites were observed upstream of RMST in three humancell lines, namely GM12878, a lymphoblastoid cell line, H1 hESCs,...
... of pluripotent stem cells from adult human fibroblasts by defined factors Cell 131, 861-872 Takahashi, K., and Yamanaka, S (2006) Induction of pluripotent stem cells from mouse embryonic and adult ... E.R (2009) Isolation and characterization of neural crest stem cells derived from in vitro-differentiated humanembryonicstem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., ... ofhumanembryonicstem cells induced by stromal cells Stem Cells Dev 19, 71-82 Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S (2007) Induction of...
... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Humanembryonicstem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... HUMANEMBRYONICSTEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... development of the nervous system in man NSCs obtained from a diseased postmortem human fetal or adult brain can function as a cellular model to decipher the mechanisms of pathogenesis ofhuman neurological...
... function of immune cells 20 1.2.3.5 Other placenta related factors 21 iv 1.2.4 Clinical relevance of placenta development 1.3 Embryonicstem cells 1.3.1 Mouse embryonicstem cells 22 23 23 1.3.1.1 Derivation ... Embryonicstem cells 1.3.1 Mouse embryonicstem cells 1.3.1.1 Derivationof mESC mESCs were first derived in 1981 {Martin, 1981 #292; Evans, 1981 #293} mESCs are pluripotent cells derived from the ... Mouse trophoblast stem cells Mouse trophoblast cells have not been derived from mESC, however, mouse trophoblast stem (TS) celllines can be derived from the blastocysts Mouse TS cells can be maintained...
... classes of pluripotent stem cells in humans, other primates and mice These are embryonicstem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... the human Animal embryonicstem cells Embryonicstem cells were first derived from certain strains of mice (Evans & Kaufman 1981) Embryonicstemcelllines have been established from the mouse, ... manipulation ofstem cells 35 CHAPTER Derivation and Propagation ofHumanEmbryonicStemCellLines on Xeno-Free Support Systems and in the Presence of Xeno-Free Proteins 36 Introduction hESC lines...
... VS, Jones JM Embryonicstemcelllines derived fromhuman blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonicstemcelllinesfromhuman blastocysts: ... xeno-free cryopreservation method for the storage ofhumanembryonicstem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... cannot be said ofhuman embryos, which are much less robust and hardy compared to mouse embryos Hence laboratories involved in handling humanembryos in clinical assisted reproduction often have...
... Guidelines for HumanEmbryonicStemCell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMANEMBRYONICSTEMCELL RESEARCH Committee on Guidelines for HumanEmbryonicStemCell ... Guidelines for HumanEmbryonicStemCell Research http://www.nap.edu/catalog/11278.html Scientific Background ofHumanEmbryonicStemCell Research INTRODUCTION Humanembryonicstem cells (hES cells) ... derivationof hES cells fromhuman embryos, • research in which hES cells are utilized to create or contribute to a human embryo, • research utilizing hES cells that were derived fromhuman embryos...
... I6, H9 and BG01v are names ofhumanembryonicstem (hES) cells lines P denoted the number of passages of the celllines H9-EB denoted embryoid body (EB) prepared fromcell line H9 and the day indicates ... profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the celllines and five types of terminally differentiated adult cells The integration of miRNA ... names of hES cells lines P denotes the number of passages of the celllines H9-EB denotes embryoid body (EB) prepared fromcell line H9 and the day indicates the time in culture HMVEC = human...
... differentiation ofembryonicstem cells Curr Opin Cell Biol 1995, 7:862-869 10 Dani C, Smith AG, Dessolin S, Leroy P, Staccini L, Villageois P, Darimont C, Ailhaud G: Differentiation ofembryonicstem cells ... Wdziekonski B, Villageois P, Dani C: Differentiation of mouse embryonicstem cells and ofhuman adult stem cells into adipocytes Curr Protoc Cell Biol 2007, Chapter 23(Unit 23 24) 54 Irizarry RA, ... the mESC culture system might not only allow the generation of adipose stem cells, but also of their potential vascular niche, opens new avenues for the study of adipose stemcell biology and their...
... epigenomic profiling of a cohort ofhumanembryonicstemcell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual ... The combination ofstemcell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stemcell differentiation ... embryonicstem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of...
... Differentiation of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of ... structures of the peripheral nervous system, such as Schwann and glia cells of the autonomic and enteric nervous systems, endocrine cells, such as the adrenal medulla, and C-cells of the thyroid ... of gastrulation Lack of Eomes abrogates the formation ofembryonic and extra -embryonic mesoderm [42] It has been shown that Eomes is specifically required for the directed movement of cells from...
... from differentiating ES cells [5-8] Most often, protocols to enrich cardiomyocytes from transgenic cardiac celllines were optimized for ES celllines such as the D3 cell line cultivated on MEFs ... interactions Enrichment Figure of α-MHC+ cells isolated from the α-MHC+ ES cell lineage after puromycin treatment Enrichment of α-MHC+ cells isolated from the α-MHC+ ES cell lineage after puromycin ... Hescheler J, Sachinidis A: Embryonicstem cells: a promising tool for cell replacement therapy J Cell Mol Med 2004, 8:465-473 Winkler J, Hescheler J, Sachinidis A: Embryonicstem cells for basic research...
... factor HumanembryonicstemcellHumanembryonicstemcell derived dendritic cellHumanembryonicstemcell derived mesenchymal stemcellHumanembryonicstemcell derived neural stemcell Hematopoietic ... evaluation of the effects of adult stem cells on differentiation of DCs from monocytes - Used for evaluation of the effects of adult stem cells on differentiation of LPS-induced DCs from monocytes ... evaluation of the effects of adult stem cells on differentiation of DCs from monocytes - Used for evaluation of the effects of adult stem cells on differentiation of LPS-induced DCs from monocytes CD209...