ebioscience differentiation of the human hematopoietic stem cell

... per- centages of the cells in the G0 ⁄ G1, S and G2 ⁄ M phases of the cell cycle after treatment with 20 l M or 100 lM ATP for 24 h. The gat- ing used for the quantification of the cells in the G0 ⁄ ... nucleotides on cell growth, the role of nucleotides as effectors of neoplastic transformation and the ubiquitous expression of the purinergic receptors led to the investigation of the therapeutic ... Inhibition of proliferation of LXF-289 lung tumor cells. Effects of P2 receptor agonists. The influence of the different P2 receptor agonists is shown as percentage inhibition of the prolifer- ation of...

Ngày tải lên: 07/03/2014, 12:20

Ngày tải lên: 14/08/2014, 20:22

... names of human embryonic stem (hES) cells lines. P denoted the number of passages of the cell lines. H9-EB denoted embryoid body (EB) prepared from cell line H9 and the day indicates the time ... in the undifferentiation of human embryonic stem cells; among the miRNAs that are highly expressed in undifferentiated embryonic stem cells, the miR-520 cluster may be closely involved in hES cell function ... names of hES cells lines. P denotes the number of passages of the cell lines. H9-EB denotes embryoid body (EB) prepared from cell line H9 and the day indicates the time in culture. HMVEC = human...

Ngày tải lên: 18/06/2014, 15:20

Ngày tải lên: 09/08/2014, 10:23

Ngày tải lên: 09/08/2014, 14:22

... percen- tage of mononuclear cells in Additional File 1. Analysis of the number of the progenitor cells in circulation by HALO assay Cells expressing the CD34 and CD133 markers are associated with hematopoietic ... Differential mobilization of subsets of progenitor cells from the bone marrow. Cell Stem Cell 2009, 4(1):62-72. 40. Westerweel PE, Visseren FL, Hajer GR, et al: Endothelial progenitor cell levels in obese men with the ... 0 .07). These data suggest Stem- Kine supplementation increases circula- tion of cells capable of giving rise to hematopoietic- lineage cells in vitro. Stem- Kine augments circulation of cells with EPC...

Ngày tải lên: 18/06/2014, 16:20

Ngày tải lên: 10/08/2014, 05:21

Ngày tải lên: 10/08/2014, 05:21

Ngày tải lên: 13/08/2014, 16:20

Ngày tải lên: 14/08/2014, 08:20

... Becker EW. The roles of ATP in the dynamics of the actin filaments of the cytoskeleton. Biol Chem. 2006;387(4):401-6. 2. Lopez LA, Bertini F. The in vivo effect of low body temperatures on the degree ... Besides cell survivability, another critical parameter is whether the undifferentiated state of hESC is affected by exposure to low temperature. The degree of spontaneous differentiation of low ... Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown). All of the detached cells were washed off prior to the MTT assay. From the raw...

Ngày tải lên: 31/10/2012, 16:57

... region of Prm3. Deletion of the PPARc (a) and RXR III half sites to generate the )170 subfragment retained 15d-PGJ 2 -suppression of Prm3 activity. On the other hand, either deletion of the PPARc ... (DR5). These data provide direct evidence for the role of PPARc in the regulation of human TP gene expression within the vasculature and point to further critical dif- ferences in the modes of transcriptional ... 15d-PGJ 2 -suppres- sion of luciferase expression. On the other hand, either deletion of the latter PPARc (b) and RXR IV half sites, such as within the )154 subfragment, or dis- ruption of the RXR IV half...

Ngày tải lên: 07/03/2014, 21:20

... 500 sites per cell. This is approxi- mately 50% as compared to the responsive HBL cells. The lack of functional coupling of these cell lines, particularly of DOR cells homozygous for the wild-type ... shift of the displacement curve and by the lower amount of radioactivity bound in the absence of competitor (in spite of the lack of statistically significant differences in the number of binding ... gain -of- function, mutations of MC1R are indeed frequent in HMC lines, and that impairment of signaling through the cAMP cascade occurs in these cells even in the absence of mutations in the coding region of the gene....

Ngày tải lên: 17/03/2014, 10:20

... exposed to antihuman whole serum antibodies, which attach to any human cell. Cell cell connections within the outer layer of the trophoblast prevent penetration of the antibodies into the blastocyst, ... demonstrated that ESC-like cells can be isolated from human embryos. They described the isolation of ICM cells from human blastocysts and the culturing of these cells for two pas- sages, while ... and the derivation of human lines using basically the same culturing techniques. Chapter 1 Methods for the Derivation of Human Embryonic Stem Cell Lines 3 1.1 Introduction demonstrates the...

Ngày tải lên: 22/03/2014, 09:20

... Sweden Introduction Mast cells (MCs) are resident tissue cells that are dis- tributed along the surfaces of the body. They are fre- quently found in the mucosa of the airways and intestine, in connective tissue of the ... number of poten- tial substrates during the screening of the full human proteome. This highlights the importance of factors other than the extended cleavage specificity in deter- mining whether ... or Glu, but attract these residues equally well, probably solely on the basis on the negative charge of their side chains. Verifying the consensus sequence by the use of a new type of recombinant...

Ngày tải lên: 22/03/2014, 21:21

... by the redox state of the cell. Furthermore, hydrogen peroxide could in part prevent the effect of Pycnogenol Ò and thus partly restore the activity of TrxR. One possible mechanism explaining the ... were observed, as the activity of both enzymes decreased in parallel with the duration of incubation for both treated and non-treated cells, reflecting varying expression at the various stages of the cell ... agent, the regulation of expression of the anti- oxidant selenoproteins becomes much more complex. We show here that regulation of TrxR appears to be primarily dependent on the redox state of the cell, rather...

Ngày tải lên: 30/03/2014, 02:20

... characterization of transplanted cells derived from human ES cells We induced differentiation of human ES cells in an in vitro two-dimensional culture on OP9 stromal cell line and examined the expression of ... purposes) Effects of the transplanted vascular cells on the CBF in the ipsilateral sideFigure 3 Effects of the transplanted vascular cells on the CBF in the ipsilateral side. A-C: LDPI analysis of the CBF ... MCs derived from human ES cells (hES- ECs+MCs). The cell mixture was composed of almost the same number of ECs and MCs. We resorted the VE-cad- herin + cells from these expanded cells to obtain...

Ngày tải lên: 18/06/2014, 15:20

... protein has the capacity to drive cisplatin out of the CNE2 cells. ABCC2 siRNA increased the sensitivity of cisplatin in CNE2 cells without changing the cell viability To assess the cell viability, ... found between the cell growth of these cells (Fig. 3A), which indicate that the viability of cells was influenced neither by the transfection procedure, nor by ABCC2 gene. To evaluate the sensitivity ... CNE2 cells. In vitro, cellular accumulation of cisplatin was detected by HPLC. The capacity of cellular growth and sensitivity of cells against cisplatin were detected by MTT assay. In vivo, the...

Ngày tải lên: 18/06/2014, 15:20

... to the uterus [24]. Therefore, based on the experience of our research group in the identification, and character- ization of potential sources of adult stem cells [7,10,12- 15], the aim of this ... and showed the ability to differentiate into cell types of the three germ layers [17-23]. The human fallopian tubes (hFTs) share the same embry- ologic origin as the uterus. They have the capacity ... potential source of pluripotent cells for regenerative medicine. The identification of niches of tis- sue-specific stem cells capable of replacing damaged dif- ferentiated cells in the hFTs may contribute...

Ngày tải lên: 18/06/2014, 15:20