5islamic view on human embryonic stem cell research

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... Background of Human Embryonic Stem Cell Research 29 Addressing Ethical and Scientific Concerns Through Oversight 47 Current Regulation of Human Embryonic Stem Cell Research 63 Recruiting Donors and ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html 26 Guidelines for Human Embryonic Stem Cell Research Prerequisites to hES cell research (such as examination of...

Ngày tải lên: 06/03/2014, 15:20

... methylation Conserved Discordant methylation Unconserved Methylation Group Conser ved methylated Unconser ved met hylated Unmethylated Figure Conservation and DNA methylation of CG and non-CG sites ... pluripotent stem cells [15], suggesting there may be a functional role for non-CG methylation in pluripotent stem cells However, less is known about the conservation and variability of DNA methylation ... lines 10 11 12 13 Abbreviations bp: base pair; BS-seq: whole genome bisulfite sequencing; ESC: embryonic stem cells; FDR: false discovery rate; HESC: human embryonic stem cell; HGNC: HUGO Gene Nomenclature...

Ngày tải lên: 09/08/2014, 23:20

... Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonic stem cell lines from human ... primary target cells that need to be protected against HIV-1 infection Methods hES cell growth and propagation H1 human embryonic stem cell line (hES) was obtained from WiCell (Madison, Wisconsin) The ... Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop...

Ngày tải lên: 10/08/2014, 05:20

... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors and ... Annotation, Visualization and Integrated Discovery; EASE, Expression Analysis Systematic Explorer; EB, embryoid bodie; ESC, embryonic stem cell; GEO, Gene Expression Omnibus; GO, gene ontology; h, human; ... development from human embryonic stem cell lines Exp Hematol 2005, 33:987-996 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem cells progresses...

Ngày tải lên: 14/08/2014, 08:20

... Transcriptional control of stem cell pluripotency 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem cell transcriptional core 1.1.2 An expanded transcriptional regulatory ... human pluripotent stem cells 28 3.2 Expansion and mitotic inactivation of MEF cells 28 3.3 Preparation of MEF-conditioned medium 29   iii  3.4 Culture of PA6 mouse skull bone marrow stromal cells ... characterization of lncRNAs involved in differentiation has been carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... transcriptional circuitry governing stem cell pluripotency and cell fate determination, and perturbations of lncRNA levels will result in stem cell differentiation   104  Chapter VII – Long Non-coding ... representation of differentiation of ReN-VM neural stem cells following transfection of siRNAs Neural stem cells were plated one day prior to siRNA transfection Neuronal differentiation was induced ... shown Gene Ontology Biological Process 10 GO Term neurogenesis generation of neurons neuron differentiation cell morphogenesis neuron development central nervous system development cell morphogenesis...

Ngày tải lên: 09/09/2015, 17:54

... transcription of RMST (Figure 8.4) REST, also known as NRSF or neuronal restrictive silencing factor, is a transcription factor expressed in neural stem cells and non-neuronal cells, to repress neuronal ... AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas (PFSK-1) indicated ... qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated by...

Ngày tải lên: 09/09/2015, 17:54

... maintenance of human embryonic stem cells (hESCs), and human neuronal development, using hESCs as a cellular model for neural differentiation 9.1.1 Identification of functional human lncRNAs Using ... transcriptional networks during mouse embryonic stem cell selfrenewal and differentiation Cell Stem Cell 6, 153-166 Wang, K.C., and Chang, H.Y (2011) Molecular mechanisms of long noncoding RNAs Mol Cell ... Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells Nat Biotechnol 25, 1468-1475 Lee, S.M., Danielian, P.S., Fritzsch, B., and McMahon, A.P...

Ngày tải lên: 09/09/2015, 17:55

... protein Embryonic stem cell Forward scatter -Galactosylceramide Granulocyte-macrophage colony-stimulating factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic ... Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic progenitor cell Hematopoietic stem cell Herpes simplex virus Interferon- Immunoglobulin ... evaluation of the effects of adult stem cells on differentiation of DCs from monocytes - Used for evaluation of the effects of adult stem cells on differentiation of LPS-induced DCs from monocytes...

Ngày tải lên: 09/09/2015, 18:52

... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED ... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage ... loss-of-function mutations in SOX2 is telltale of the dose-dependent function of human SOX2 A reasonable explanation for the dosage and context dependence of Sox2 function is that the specificity of action...

Ngày tải lên: 10/09/2015, 09:08

... from the donor cells to the host, reducing host immune responses by limiting immunogen exposure and retention in donor cells, reducing the risk of tumour generation upon implantation and attaining ... medium Matrigel with MEF Conditioned Medium hESCs can be propagated for long periods of time on Matrigel with conditioned medium (up to 130 population doublings) [4] Conditioned medium is obtained ... large-scale expansion of hESCs using Matrigel with conditioned medium still faces the same problem faced by the standard feeder culture system Secondly, the use of conditioned medium from non -human MEFs...

Ngày tải lên: 11/09/2015, 10:06

... Prelamin-A/C 100.00% 9.04% Histone H2B type 1-L 99.90% 20.60% 2.54% 100.00% 100.00% 1.89% 67804 Moesin* 100.00% 7.45% 22333 22564 22470 74123 Histone H1.3 Histone H1.5 Histone H1x* Isoform A of PrelaminA/C ... 7.81% Table 2: Consolidated list of protein identifications compiled from mass spectrometric analysis of DxSDOC and DxSDOCDOC matrices Mass spectrometry analysis was done in collaboration with Christopher ... alpha-2(VI) chain Thrombospondin-1* Vitronectin* 100.00% 12.40% Isoform of Tenascin*† Isoform of Transglutaminase 100.00% 10.50% 99.80% 4.61% 100.00% 99.80% 8.29% 8.79% Thrombospondin-1* 100.00% 6.50%...

Ngày tải lên: 11/09/2015, 10:06

... immunofluoresence comparison, section 3.3.2), it would be expected that the 52 increased presence of Fn would aid in increasing integrin binding and hence cell binding This contradiction could possibly ... from each matrix condition were sacrificed for cell counting using a haemacytometer Population doublings from each passage were calculated and the data plotted for comparison (Figure 5A) 53 54 ... has been optimized for Matrigel, the standard feeder-free culture system Subculture was done every 5-7 days depending on the confluency of the hESCs In this way, hESCs were maintained for up to...

Ngày tải lên: 11/09/2015, 10:06

... cells Although hESCs on DxSDOCDOC did have some spontaneously differentiated cells in the population, these cells were not common Colonies of hESCs on DxSDOCDOC were larger in the later passage compared ... the embryonic ectoderm at the cylinder stage, and down-regulated at gastrulation in an anterior-posterior pattern Germ cells continue to maintain the expression of Oct-4 until the initiation of ... three conditions showed comparable expression, except for Oct-4 (Figure 5D) Oct-4 positive cells were found to be present in 47.2% of hESCs cultured on Matrigel, compared to only 23.4% of hESCs on...

Ngày tải lên: 11/09/2015, 10:07

... differentiation state or potency of stem cells in comparison to differentiated cells, DNA methylation studies were done The goal was to identify methylation changes between hES cells grown on the human ... metaphase showed non-clonal random loss Again, as mentioned in section 3.5.6, these cells can be considered as karyotypically normal due to only metaphase out of 20 having non-clonal random loss, ... National Stem Cell Bank In this protocol, the hESCs formed embryoid bodies using non-adherent cell culture plates and were transferred onto laminincoated plates and incubated in neural induction...

Ngày tải lên: 11/09/2015, 10:07

... Marshall VS Embryonic stem cell lines derived from human blastocysts Science 282:1145–1147 (1998) Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: ... made at each human embryonic stem cell culture step that can contribute to a safer and more efficient human embryonic stem cell culture: For the enhancement of collagenous extracellular matrix, ... signatures of seven individual uman embryonic stem cell lines Stem Cells 23:1343-1356 (2005) Stewart R, Stojkovic M, Lako M Mechanisms of self-renewal in human embryonic stem cells Eur J Cancer 40:1257-1272...

Ngày tải lên: 11/09/2015, 10:07

... Australia) A Human Embryonic Stem Cell based Model of Trophoblast Formation (manuscript in preparation, 2009) Inhibition of methylation releases the developmental plasticity of mouse embryonic stem cells ... placentation will be immediately impactful in the field of ART and later clinical complications related to the pregnancy 23 1.3 Embryonic stem cells 1.3.1 Mouse embryonic stem cells 1.3.1.1 Derivation ... Transcription factors 25 1.3.1.4 Mouse trophoblast Stem cells 27 1.3.2 Human embryonic stem cell (hESC) 27 1.3.2.1 hESC derivation 27 1.3.2.2 hESC culture condition 30 1.3.2.3 Core signaling pathways of...

Ngày tải lên: 11/09/2015, 16:07

... formation 0.2 1.5 [66] Angiogenesis 0.0 1.6 [67,68] Regulation of cell differentiation 0.8 1.5 [67,68] Cell growth 2.3 1.5 [67,68] Regulation of cell migration Muscle cell differentiation 2.0 ... KR: Embryonic stem cells as a model to study cardiac, skeletal muscle, and vascular smooth muscle cell differentiation Methods Mol Biol 2002, 185:127-156 Wobus AM, Boheler KR: Embryonic stem cells: ... process 0.6 7.3 [78] Cell morphogenesis 0.0 1.6 [79] Regulation of progression through cell cycle 0.0 1.7 [79] Regulation of cell differentiation 0.4 1.9 [79] Cell migration Aire [68] Placenta...

Ngày tải lên: 09/08/2014, 20:22

... stem cells versus embryonic stem cells The general differences between the characteristics of adult and embryonic stem cells are summarised in Table The contention that somatic stem cells alone ... pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of pluripotent stem ... ICM on an embryonic fibroblast feeder layer (human or murine) leads to the 19 development of an embryonic stem cell line In nature however, embryonic stem cells are ephemeral and present only...

Ngày tải lên: 16/09/2015, 15:55