Turning of collagen i fibrillogenesis kinetics via macromolecular crowding

... the kinetics of collagen I assembly 95 III-4.2 The entropic contribution of crowding to fibrillogenesis 97 III-4.3 Long-range synergistic effects in mixtures of crowders 99 III-5 ... conditions in vitro, with the aim of mimicking in vivo conditions (Chen, Loe et al 2011) A central premise of the macromolecular crowding theory is the inertia of crowders, i. e their i...

Ngày tải lên: 09/09/2015, 11:28

... values TGF-b1 signalling was affected similarly, with no effect by inhibiting PLC-b (Fig 5A), strong down -regulation of collagen I levels after inhibition of PC-PLC (Fig 5B), and similar collagen I ... subunit of Gai [45] was inhibited specifically by U73122 [46] Inhibiting PLC-b by U73122 did not alter ET-1- induced collagen I synthesis, nor did it influence b...

Ngày tải lên: 23/03/2014, 11:20

... expression and image analysis of type I and III collagen protein precursors Immunohistochemical staining of the precursor protein of collagen I and III was present mainly extracellularly, being visible ... Precursor proteins of collagen I and III The immunohistochemical expression of precursor proteins of collagen I and III was quant...

Ngày tải lên: 12/08/2014, 13:22

... (1999) Tissue inhibitor of matrix metalloproteinase-2 regulates matrix metalloproteinase-2 activation by modulation of membrane-type matrix metalloproteinase activity in high and low invasive melanoma ... cysteinerich protein with kazal motifs (RECK) is a cell membrane associated inhibitor of MMP-2 that is able to inhibit the second activation step of MMP-2 [9] The s...

Ngày tải lên: 18/02/2014, 11:20

... stoichiometries of participation of water, cations and anions in specific and non-specific binding of lac repressor to DNA Possible thermodynamic origins of the ‘‘glutamate effect’’ on protein–DNA ... function optimally Effects of mutations The point mutations are not in the immediate vicinity of the active site situated at the bottom of the positively charge...

Ngày tải lên: 16/03/2014, 06:20

... parts of the joint and, in keeping with this, col II and YKL40 expression rose in all locations after ACLT in our study Surprisingly, we detected significant differences in col II and col I expression ... sensitive and quantitative molecular analysis of the response of articular cartilage to increased joint instability and altered mechanical loading, and...

Ngày tải lên: 09/08/2014, 06:22

... tin, m i g i tin có giá trị offset riêng truyền theo nhiều đường để t i đích T i đích, nhờ vào giá trị offset g i tin mà liệu l i kết hợp l i ban đầu L i dụng i u này, hacker tạo nhiều g i tin ... quyền hạn thân kẻ công thiết bị hệ thống mạng để tiếp cận phá ho i (các router, switch…) III/ Các cách phòng chống DoS làm tiêu tốn nhiều th i gian tiền bạc, vậy, cần ph i có biện pháp đ...

Ngày tải lên: 28/10/2013, 12:15

... feelings of estrangement from the world of their parents and their own desire to make their lives anew On the other hand, they simply could not buy into what they saw as Fichte s one-sidedness, ... Part II The revolution continued: post-Kantians to the ordinary, the dignity of the unacquainted to that of which we are acquainted, the mere appearance of infinity to finite...

Ngày tải lên: 01/11/2013, 08:20

... while in the second case, this applies to the nonequilibrium evolution of a vibrated granular bed 102 Shaking a box of sand I – a simple lattice model Table 7.1 Two different nonequilibrium regimes ... 100 Shaking a box of sand I – a simple lattice model ageing, and many other glassy phenomena, is still somewhat mysterious, although ongoing...

Ngày tải lên: 01/11/2013, 08:20

... observations of the Councillor sounded to him too daring and phantastical They looked at one another from the crown of the head to the soles of the feet; and when matters grew to too high a pitch, then the ... herself, as then she is sure of having it done properly They were telling each other, with a confidential interchange of ideas, where they had been during the da...

Ngày tải lên: 07/11/2013, 07:15

... Dimension area of the HOLE dialog box, the Technologies, USA For services, © CADCIM Technologies, USA For engineering ser vices, contact sales@cadcim.com Options Aiding Construction of Parts-I Technologies, ... modify the references of a feature Technologies, USA For services, © CADCIM Technologies, USA For engineering ser vices, contact sales@cadcim.com Options Aiding Cons...

Ngày tải lên: 03/01/2014, 23:56

... and plasmid were analyzed using linearized pBAD ⁄ gIII plasmid, linearized and denatured plasmid and intact plasmid The pBAD ⁄ gIII plasmid was linearized using SalI and denatured by incubation ... Biochemical Adaptation Princeton University Press, Princeton, NJ Wu SI, Lo SK, Shao CP, Tsai HW & Hor LI (2001) Cloning and characterization of a periplasmic nuclease of Vibrio vuln...

Ngày tải lên: 19/02/2014, 05:20

... structural modification in these regions The N-terminal region of TnI, positions 1–95, seems to have mainly a structural function in maintaining the organization of the Tn [7–9,45] The function of the C-terminal ... part of the signal This indicates that the high affinity Ca2+ signal is related to the N-domain Further, the disruption of the sites in the C-d...

Ngày tải lên: 20/02/2014, 11:20

... involvement of ADH1 and ADH4 in ethanol oxidation in brain tissue Regarding retinoid metabolism in the adult brain, enzymes other than ADH4 must be active, because in vivo and in vitro data indicate ... Table Distribution of the three ADH classes in adult rat, mouse and human brain tissue The presence of specific signal is shown by +, strong prese...

Ngày tải lên: 21/02/2014, 00:20

... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of si...

Ngày tải lên: 21/02/2014, 03:20