degradation products of type i collagen

Báo cáo khoa học: Involvement of lysine 1047 in type I collagen-mediated activation of polymorphonuclear neutrophils doc

Ngày tải lên : 07/03/2014, 06:20

... because it induced a dose-dependent inhibition of ROS production, indicating the impairment of the interaction This inhibitory effect was independent of any direct scavenger effect of 3230 B Fig Inﬂuence ... (contained within the cyclic peptide CTRKKHDNAQC derived from jararhagin disintegrin) is essential for binding to the I domain of a2 integrins [24] Similarly, members of the ‘lysine–threonine–serine ... A, Wiman B & Pap S (2003) Identiﬁcation of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin Eur J Biochem 270, 2023–2029 Panizzi P,...

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Báo cáo y học: " Characterisation of the immune response to type I collagen in scleroderma" pps

Ngày tải lên : 09/08/2014, 08:22

... G: Investigation of type I and type III collagens of the lung in progressive systemic sclerosis Arthritis Rheum 1981, 24:625-631 White B: Immunopathogenesis of systemic sclerosis Rheum Dis Clin ... induction to type I collagen (CI) significantly reduces the skin score in patients with diffuse systemic sclerosis (SSc) with late-phase disease Results of a NIAMS/ NIAID multicenter phase II ... Kang AH, Postlethwaite AE: Induction of immune tolerance to human type I collagen in patients with systemic sclerosis by oral administration of bovine type I collagen Arthritis Rheum 2000, 43:1054-1061...

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Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc

Ngày tải lên : 21/02/2014, 03:20

... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW Cloning of sipW ... cut with either PstI and EcoRI in case of sipV or EcoRI-SacI in case of sipW and ligated into corresponding sites of pUC18 DNA The ligation mixes were used for PCR with oligo nucleotides Uni1 or...

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Báo cáo khoa học: Promoters of type I interferon genes from Atlantic salmon contain two main regulatory regions docx

Ngày tải lên : 07/03/2014, 12:20

... role in the activation of the salmon IFN promoter, but it cannot act alone to initiate transcription A hallmark of mammalian IFN-a ⁄ b is their rapid induction by virus infection mainly because of ... shown (Fig 7) PDTC is a metal chelator with antioxidant properties which speciﬁcally inhibits NFjB-induced pathways [39] Secondly, partial inhibition of promoter activity by 2-AP (Fig 7), indicates ... Atlantic salmon A role for NFjB in the activation of PR -I was further supported by two different inhibitor experiments First, dose-dependent inhibition of promoter activity by the NFjB inhibitor...

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Báo cáo khoa học: A comparative study of type I and type II tryparedoxin peroxidases in Leishmania major pot

Ngày tải lên : 23/03/2014, 07:20

... TryX indicating that formation of an intramolecular disulde is important for interaction with TryX In contrast, this mutant displayed signicant activity with dithiothreitol (65% of wild -type) suggesting ... reduced with 10 mm dithiothreitol or oxidized with two equivalents of hydrogen peroxide, respectively Dithiothreitol, trace amounts of oxidized dithiothreitol or hydrogen peroxide did not inuence ... partial mobility shifts under reducing and oxidizing conditions by SDS PAGE with some higher aggregate formation evident following peroxide treatment (Fig 7) Abrogation of the mobility shift is...

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báo cáo hóa học:" Differential expression of type X collagen in a mechanically active 3-D chondrocyte culture system: a quantitative study" docx

Ngày tải lên : 20/06/2014, 00:20

... region 10 Strain (%) 0 10 12 Initial Marker Position (mm) Figure Distribution of surface strains in a typical sponge (4.3% overall strain in this example) Distribution of surface strains in a ... threshold of cyclic mechanical induction of type X collagen mRNA production is greater than 2.5% local strain This in vitro observation may have implications for the in vivo situations in http://www.josr-online.com/content/1/1/15 ... stress experienced within joint cartilage could be responsible for differential activation of genes involved in matrix remodeling In support of this hypothesis, application of mechanical stress...

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Báo cáo hóa học: " Wild type measles virus attenuation independent of type I IFN" pdf

Ngày tải lên : 20/06/2014, 01:20

... ELISA Infection with UV-inactivated virus did not induce antibody production, strongly suggesting that generation of antibodies requires initial replication of G954-V13 after intranasal infection ... http://www.virologyj.com/content/5/1/22 Figure Adaptation to a specific cell type limits replication of MV Adaptation to a specific cell type limits replication of MV Replication kinetics of MVs in PBMCs ... infection and type I IFN addition] However, high doses of type I IFN could inhibit the G954-V13 strain infection [(F): hours between infection and type I IFN addition] Ed-Zagreb infections of both...

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Báo cáo toán học: " On Systems of Quasivariational Inclusion Problems of Type I and Related Problems" potx

Ngày tải lên : 06/08/2014, 05:20

... a solution of one of the above systems, under some additional conditions, is also a solution of some other systems of quasi-optimization problems, quasi-equilibrium problems, quasivariational ... of this paper is to prove some new results on the existence of solutions to systems concerning the following quasivariational inclusions (UQVIP), Upper quasivariational inclusion problem of type ... short) if y − x ∈ C for every y ∈ A The set of ideal minimal points of A is denoted by IMin(A/C) (ii) x ∈ A is an eﬃcient (or Pareto–minimal, or nondominated) point of A w.r.t C if there is no...

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Báo cáo y học: " Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen" pptx

Ngày tải lên : 09/08/2014, 01:23

... arthritis in mice that received oral type II collagen (CII) before arthritis induction Mice that were given six consecutive administrations of 100 µg CII orally before induction of arthritis exhibited ... considered statistically significant Results Inhibition of arthritis development in tolerized CIA mice The severity of arthritis remained low both in the tolerized and nontolerized CIA mice until ... whereas IgG2a is known to be a mediator of inflammation Likewise, the level of IgG2a has been shown to be significantly increased in DBA/1 mice with CIA [12,13] We investiga- Suppression of arthritis...

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Báo cáo y học: "Antibodies against the CB10 fragment of type II collagen in rheumatoid arthritis" pdf

Ngày tải lên : 09/08/2014, 01:23

... CNBr digestion of bovine CII and purification of CB10 and CB11 peptides CNBr digestion of bovine CII was performed with mg of purified bovine CII This was dissolved in ml of 70% (v/v) formic acid, ... Holmdahl R: Epitope-specific recognition of type II collagen by rheumatoid arthritis antibodies is shared with recognition by antibodies that are arthritogenic in collagen- induced arthritis in the mouse ... antibodies against bovine type II collagen (CII) and human CII Scatter plot of the levels of IgG antibodies against human CII and bovine CII in sera of patients with rheumatoid arthritis (RA; n =...

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Báo cáo y học: "Inhibition of macropinocytosis blocks antigen presentation of type II collagen in vitro and in vivo in HLA-DR1 transgenic mice" docx

Ngày tải lên : 09/08/2014, 08:22

... (CII)-specific T cell proliferation in vivo, groups of four mice were footpad immunized with CII emulsified in TiterMax adjuvant in the absence (no inhibitor) or presence of 150 μg/mouse amiloride ... amiloride in vivo Our data show that pretreatment of professional APCs with amiloride prevents activation of CII-specific T cells in vitro We also confirmed the effect of amiloride on CII-specific T cell ... presentation to T cells could be prevented in vivo Discussion The effect of inhibitors of uptake on T cell proliferation in vivo To test vivo the effect of amiloride on mitogenic and type II collagen...

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Báo cáo y học: "Analog peptides of type II collagen can suppress arthritis in HLA-DR4" docx

Ngày tải lên : 09/08/2014, 08:22

... by the Institutional Animal Care and Use Committee Preparation of CII Native CII was solubilized from bovine articular cartilage by limited pepsin digestion and purified as described previously ... Synthesis of analog peptides of CII The peptide representing the immunodominant determinant of CII and its analog peptides containing specific amino acid substitutions were chemically synthesized ... arthritis, we used a panel of analog peptides containing substitutions at each position of the immunodominant DR4-restricted determinant of CII, replacing the wild -type residue with either alanine...

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Báo cáo y học: "Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affinity" pps

Ngày tải lên : 11/08/2014, 03:20

... S, Holmdahl R, Kleinau S: Induction of arthritis by single monoclonal IgG anti -collagen type II antibodies and enhancement of arthritis in mice lacking inhibitory FcγRIIB Eur J Immunol 2003, 33:2269-2277 ... cells of DBA/1J mice immunized with chicken CII (Sigma-Aldrich Fine Chemicals, MI, USA) emulsified with CFA (Difco Laboratories, Detroit, MI, USA) as described previously [16,18] Briefly, mice ... Time course of changes in the arthritis score induced by arthritogenic mAbs First, we investigated whether arthritis is induced by combinations of CII-3, CII-6, C2B-14, and CM-5 in DBA/1J mice...

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Báo cáo y học: "Arthrogenicity of type II collagen monoclonal antibodies associated with complement activation and antigen affini" doc

Ngày tải lên : 11/08/2014, 06:23

... S, Holmdahl R, Kleinau S: Induction of arthritis by single monoclonal IgG anti -collagen type II antibodies and enhancement of arthritis in mice lacking inhibitory FcγRIIB Eur J Immunol 2003, 33:2269-2277 ... cells of DBA/1J mice immunized with chicken CII (Sigma-Aldrich Fine Chemicals, MI, USA) emulsified with CFA (Difco Laboratories, Detroit, MI, USA) as described previously [16,18] Briefly, mice ... Time course of changes in the arthritis score induced by arthritogenic mAbs First, we investigated whether arthritis is induced by combinations of CII-3, CII-6, C2B-14, and CM-5 in DBA/1J mice...

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Dynamic studies of type i and type II cadherins EC domains

Ngày tải lên : 09/09/2015, 11:16

... chemical modification was not utilized in the unbinding experiments are i) it shows a high probability of non-specific binding, which cannot be distinguished from cadherin interactions ii) it ... cadherins form cadherin-catenin complex with catenins and p120, no vinculin is recruited B) In the released state, the vinculin binding site in α-catenin is inhibited by a putative inhibitory domain ... interaction Biotin-NTA Strepavidin-NTA NH2-NTA strepavidin BSA Strepavidin Biotin Biotin-strepavidin interaction SVA NH2 NTA SiO2 Figure 2.2 Chemical modification method for AFM unbinding experiments...

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Analysis of type IX collagen in zebrafish axial development

Ngày tải lên : 15/09/2015, 22:06

... most important types of fibrillar collagen are Types I, II, III, V and XI Type I is the most abundant collagen type, constituting 90% of fibrillar collagens It forms the largest and strongest fibrillar ... showing the different phenotypes obtained after translation Inhibition of ColIXα2 based on expression of twhh Table 4.1 Overview of expression profile of various chains of collagen IX in different ... post-segmentation period 106 Translation inhibition of colIXa2 affects early development of zebrafish 111 Fig 3.14 colIXα2 plays a role in establishing integrity of axial mesoderm 115 Fig 3.15 Inhibition of...

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DISCOVERY OF NOVEL REGULATORS OF TYPE i INTERFERON RESPONSE

Ngày tải lên : 04/10/2015, 16:03

... the escalating of infection inside the host (Schoggins & Rice, 2011) 15 The ubiquitination mediated regulation of cell signalling The ubiquitin ligases and ubiquitination Ubiquitination is a post ... translational modification of proteins Ubiquitination regulates multiple aspects of protein functioning such as stability, signalling complex formation or dissociation etc Ubiquitination is the ... understanding of the role of ubiquitin ligases and genes promoting ubiquitination (e.g., adaptors of ubiquitination) in TLR mediated IFN response is not available till date Particularly, till now,...

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Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx

Ngày tải lên : 07/03/2014, 16:20

... chips and with 0.3 M NaCl in the case of immobilized type VI collagen To form a complex consisting of decorin, type I and type VI collagen, type VI collagen was ﬁrst immobilized After binding of ... cells WT, Wild -type Type I collagen binding Type Type Type Type Fig Surface plasmon resonance measurement of immobilized pepsin digested type VI collagen (A) Interaction with wild -type decorin core ... collagen, white cylinder (A) Interaction involving dimerization of decorin and binding of both collagens to the inner surface of decorin (B) Interaction of type I collagen with the inner surface of the...

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Báo cáo y học: "Early and stable upregulation of collagen type II, collagen type I and YKL40 expression levels in cartilage during early experimental osteoarthritis occurs independent of joint location and histological grading" ppt

Ngày tải lên : 09/08/2014, 06:22

... surgery In keeping with our observations in the tibial plateau, expression of col II and of YKL40 was significantly higher in osteoarthritic cartilage than in control cartilage, while col I was highly ... purified using a commercially available kit (PCR purification kit; Qiagen, Hilden, Germany) In a pilot study, tissue requirements had been minimized to confine the analysis to the close proximity ... this suggestion [10,13] Knee instability is very likely to increase inappropriate mechanical loading in many parts of the joint and, in keeping with this, col II and YKL40 expression rose in...

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Báo cáo y học: " Type XVIII collagen degradation products in acute lung injury" pot

Ngày tải lên : 13/08/2014, 16:20

... LONG huXVIII antibody Immunoprecipitation with anti-ALL antibody and detection with antiLONG huXVIII antibody Elevated type XVIII collagen precursors in the plasma of ALI patients compared with normal ... collagen XVIII antibodies were visualised with ECL western blotting detection reagents For binding sites of antibodies to type XVIII collagen see Figure Statistical methods The Ryan-Joiner normality ... UK) For binding sites of antibodies to type XVIII collagen see Figure Immunoprecipitation and western blotting of plasma samples Collagen XVIII was immunoprecipitated from human plasma using a monoclonal...

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